LOCUS       NZ_CP085768             4047 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 49589CZ plasmid p49589CZ_1, complete
            sequence.
ACCESSION   NZ_CP085768
VERSION     NZ_CP085768.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377414
            Assembly: GCF_020695685.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 468x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695685.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:00:36
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,076
            CDSs (total)                      :: 4,958
            Genes (coding)                    :: 4,822
            CDSs (with protein)               :: 4,822
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 58 of 136
            Pseudo Genes (incomplete)         :: 82 of 136
            Pseudo Genes (internal stop)      :: 26 of 136
            Pseudo Genes (multiple problems)  :: 26 of 136
            Pseudo Genes (short protein)      :: 1 of 136
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085768.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="49589CZ"
                     /isolation_source="Decubitus swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p49589CZ_1"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2019-03"
                     /collected_by="Fakultni nemocnice Kralovske Vinohrady"
     gene            join(3867..4047,1..1232)
                     /locus_tag="LJU37_RS22710"
                     /old_locus_tag="LJU37_22710"
     CDS             join(3867..4047,1..1232)
                     /locus_tag="LJU37_RS22710"
                     /old_locus_tag="LJU37_22710"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_113519630.1"
                     /translation="MIRLKKVFNLTEEEIVLTNEIAEAISKEPTVETHEVNINFIDVLP
                     IIPNPHNLGLSKDIDPLSLIYSKFITQTDIPPALPVFSFLSYLSAFSVNNNIMYKHPTS
                     PADYLNTWTLILAPSGAAKTTAAKIISSSIPKDIEENPMIKPNFEGADGSAAFISELAK
                     AEKKIDNFGKPIQPIFWIEDEYSQFMKKLMPGGSMVETRKTMLKIHDNDKARRVTKNDT
                     IETESIVMSGLFLNTIDSFARNFDQESINDGLGRRHNFVYAERGEKVVPTWTVDQIIES
                     LKEGLDDFFTTVKTNVIYTYSPECRKIYDHFYMVYKEKFDHILGEETNGTFFRTYFMLS
                     WKYAAIYHILLKQEGTEIQAKSFDYGIKVSLMFLSSIKRFLDYKVDLKSGAKQVRKEYL
                     QKAAEKELNNLQKYKEFLLDNIEISLRDFNRKFNIKKIESLKILKTIRENKLIKYHPLF
                     EVLAKEEAKAKK"
                     /name="hypothetical protein"
     gene            1485..1832
                     /gene="mobC"
                     /locus_tag="LJU37_RS22715"
                     /old_locus_tag="LJU37_22715"
     CDS             1485..1832
                     /gene="mobC"
                     /locus_tag="LJU37_RS22715"
                     /old_locus_tag="LJU37_22715"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048295903.1"
                     /translation="MSYKDRYNRKHSITLCFNDEEFAQVEELASMLKKPRATAVRDLIL
                     NKDFKLKKEIAKNKTGDVYFELHKIGVNINQIARAINTDIDKFISHEFSEDFLNLFEEI
                     TNEIEEIKRRI"
                     /name="mobC"
     gene            1834..3252
                     /locus_tag="LJU37_RS22720"
                     /old_locus_tag="LJU37_22720"
     CDS             1834..3252
                     /locus_tag="LJU37_RS22720"
                     /old_locus_tag="LJU37_22720"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227788612.1"
                     /translation="MYHKIIGGKKSKGQRNIKKSIEYLLREKNPEQQKDIKILSTTTKQ
                     DLINFENYILDKNKSNPYVCGVLSFEEKSISEELKTKLITEFEDILFAGIEQENRPPVM
                     WVEHNDKGRVELNYLTFNSLQDRRSYTVYLDKRDRGLVNNYSEIINYENNLSSPFEEKE
                     NKNTLTNKPKTNIPEDKKQFIENINTEIMALIINEELENREQTIKYIENMKGVKINRIR
                     KNQISIKSDLFDDDKPIVLKGDIYEEGRDYSDYRKEFNPKPTRDPEFVREKLTELKRTF
                     AERIEKREIRNRSKFKKPSLKNIPENRQSVQREDQNIGYKYTNGDFINVSNNDGFSGMY
                     IRTDNMEIVNNESINTEYKTKTATEETAGTTRTRDIEKINQQQQFVNSRLEEAGRKQQQ
                     FRKSIDTTEGRFGVCRRSVHSIISTFGRYIRTFSAYTRKNEHRQKFEKRRKIEEEKLKI
                     KNQGSYNRRRYKPY"
                     /name="hypothetical protein"
ORIGIN
        1 cattatcttt aatatattct aaatttatta ctcaaacaga tataccgcct gctttacctg
       61 tattctcttt tttaagttat ttaagtgctt ttagtgttaa taataatata atgtataaac
      121 accctacaag cccagcggat tacttgaata cttggacttt aatacttgca ccatcaggtg
      181 cagcaaaaac cacagcagcc aaaattattt caagctctat accaaaagat atagaagaaa
      241 acccaatgat taaacccaat tttgaaggtg ctgacggttc ggcagcattc atttcagagt
      301 tggcaaaagc agaaaagaaa attgataatt tcggaaaacc aatacagccg attttctgga
      361 tagaagacga atactcccag tttatgaaaa aattaatgcc gggaggttct atggttgaaa
      421 ccagaaaaac gatgttaaaa atccatgata atgataaagc ccgtagagtt acgaaaaacg
      481 atactattga aactgaaagt atcgttatga gtggtttatt tttgaatact attgatagtt
      541 ttgcgagaaa ctttgatcag gaaagtatta atgatgggtt aggaagaaga cataattttg
      601 tttatgctga aagaggggaa aaagttgtcc caacttggac agtagatcag atcatcgaat
      661 ctctcaaaga gggattagat gatttcttca ctactgttaa aacaaacgta atctatacat
      721 attccccaga atgtagaaag atttatgatc atttttatat ggtttataag gaaaaatttg
      781 atcatatatt aggagaagaa acaaacggaa cattcttcag aacttatttt atgttgagtt
      841 ggaaatatgc agcaatatat catattttat taaaacaaga aggaaccgag attcaggcaa
      901 aatcttttga ttatgggatt aaagtttcat taatgttttt atccagtata aaaaggtttt
      961 tagattataa agtagattta aaatctggtg ctaaacaagt aaggaaagaa tatttacaaa
     1021 aagcagcaga aaaagaatta aataatttac aaaaatacaa agagtttttg cttgataata
     1081 ttgaaatttc attaagagac ttcaatagaa aatttaatat taaaaagatc gaaagtctaa
     1141 aaatactcaa aacaatcaga gaaaataaat taataaaata tcatccattg tttgaagtgt
     1201 tagcaaaaga agaagccaaa gcaaaaaaat aaaaccgtcc taccggacag gtcaacccct
     1261 cacaaaaaag ccattttaca aatggcttta tttgttcacg gttatatata aaacccaaat
     1321 ttgatttgat tattttttat tcaggtattg acttaatttt tatatgtatt aattaatata
     1381 taagtggaaa actatataac agcaagctca gacaatactt cgtattgtcg cttgtgaggg
     1441 gttccccctt caatcccctt aaaacccaac acaggaggca taggatgagc tacaaggata
     1501 gatacaacag gaagcacagc ataaccctgt gcttcaatga tgaagaattt gcacaggttg
     1561 aagaactggc ttctatgttg aaaaaaccca gagcaacagc cgttagagat cttattctca
     1621 ataaagattt taaactcaaa aaagaaatag caaaaaataa aactggtgat gtatattttg
     1681 aactccataa aataggagta aacattaacc agattgcaag agcaataaat actgatattg
     1741 ataaatttat ttcccatgag ttttcagaag attttttaaa tttgtttgag gaaataacaa
     1801 atgagataga agaaataaaa aggagaatat aatatgtacc ataaaataat aggaggtaaa
     1861 aaaagcaaag gtcaaagaaa tataaaaaaa agtattgaat atttattgag agagaaaaac
     1921 ccagaacaac agaaagacat aaaaatatta tcaacaacca caaaacaaga cctaatcaat
     1981 tttgaaaatt atattttaga taaaaacaaa tcaaacccat atgtgtgtgg tgttttatca
     2041 tttgaagaaa aatccatatc cgaagaatta aaaactaaat taattacaga gtttgaagat
     2101 attttatttg cagggattga acaagaaaac agacctccag ttatgtgggt agaacacaac
     2161 gacaagggca gggtagagtt aaattattta acatttaaca gtttacaaga cagaagatca
     2221 tatactgtat atttagataa aagagatcgt ggattggtaa ataactactc tgaaataatt
     2281 aattatgaaa ataatttatc aagtccattc gaagagaaag aaaataaaaa cacattaacc
     2341 aacaaaccaa aaaccaatat tccagaagat aaaaaacaat ttattgaaaa tattaatact
     2401 gaaataatgg cattaattat taatgaagag ttggaaaata gagaacaaac catcaaatat
     2461 attgaaaata tgaaaggtgt taaaataaac agaatacgaa aaaatcaaat atcaattaaa
     2521 tctgatttgt ttgatgacga taaacccatt gtcctgaaag gagatatata tgaagaggga
     2581 agagattaca gcgattatag aaaagaattc aacccaaaac ctacaagaga tccagagttt
     2641 gttagagaaa aactcacaga gcttaaaaga acatttgcag aaagaattga aaaacgagaa
     2701 atcagaaata gatcaaagtt taaaaaacca tcgctcaaaa atataccaga aaatagacaa
     2761 agtgttcaaa gagaagatca aaatattgga tataaatata ctaacggcga ttttatcaat
     2821 gttagtaata acgatggttt ttcaggtatg tatattcgca ctgataatat ggagattgta
     2881 aacaatgagt caattaatac agaatataaa actaaaacag caacagaaga aacagcagga
     2941 actacaagaa caagagatat tgaaaaaatt aatcagcaac aacaatttgt taattcgaga
     3001 cttgaggaag ctggaagaaa acaacagcaa ttcagaaagt cgattgatac aactgaggga
     3061 agatttggag tatgtagaag atctgtacac tcaattattt ctacttttgg aagatacata
     3121 cgaacgtttt cagcatatac aaggaagaat gaacacagac aaaaattcga aaaaagacga
     3181 aaaatcgaag aagaaaaact caaaatcaaa aatcagggaa gttacaacag aagacgatat
     3241 aaaccatatt aaaaatatga tgacaacact actagatcac tatgataaaa ctttaaccgt
     3301 gaacaagtga gagccagccg tcaggctggt tcttgcttgt gaggggttga cctgtccggt
     3361 aggacggttt tctgtcagaa atctgtcaga gtgtcagaaa aaaccaagaa aacttgtcag
     3421 atattttcca aaaaagatcc gacaccttaa aatccattga gtcaaaaata aaaacgggat
     3481 attttatgac aaaacaggtt ataaaattgc ctgttttttt aatttttgat tttgtcataa
     3541 aactaataac accgagaaag attatttttt ctttatatat caatacatta cgaaaaatgt
     3601 cctctctttt gtcaaaggtt gttagatttt ttatgacaaa tctatgacaa aatttttttt
     3661 aaacccttta aaatcagtat gttacgtgat ttgtcataat gtcatagtgt caaacagggg
     3721 gggggtggta tgtttttggc ttcgcaaaaa ccaaccaaaa aataagacat tttggaatgt
     3781 gtcatatttt ttgtaaaaat atcattgaca agttaaaacc ttatgttata actaattata
     3841 taatagatat aataggagat tttattatga taagattaaa aaaagttttt aatttaacag
     3901 aagaagaaat agttttaaca aacgaaatag cagaagcaat tagcaaagag ccaacagtag
     3961 aaacccacga agtgaatata aattttattg atgttttacc tataatacca aacccccata
     4021 atttaggttt atcaaaagac atagatc
//
