LOCUS       NZ_CP085762             9402 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 54569CZ plasmid p54569CZ_IncQ1,
            complete sequence.
ACCESSION   NZ_CP085762
VERSION     NZ_CP085762.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377422
            Assembly: GCF_020695605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695605.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:44:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,550
            Genes (coding)                    :: 4,432
            CDSs (with protein)               :: 4,432
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 43 of 118
            Pseudo Genes (incomplete)         :: 75 of 118
            Pseudo Genes (internal stop)      :: 18 of 118
            Pseudo Genes (multiple problems)  :: 17 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085762.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="54569CZ"
                     /isolation_source="wound abdomin"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p54569CZ_IncQ1"
                     /geo_loc_name="Czech Republic: Hradec Kralove Region"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-11"
                     /collected_by="Fakultni nemocnice Hradec Kralove"
     gene            complement(8..787)
                     /locus_tag="LJU41_RS22275"
                     /old_locus_tag="LJU41_22275"
     CDS             complement(8..787)
                     /locus_tag="LJU41_RS22275"
                     /old_locus_tag="LJU41_22275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="WP_058199817.1"
                     /translation="MELPNIIQQFIGNSVLEPNKIGQSPSDVYSFNRNNETFFLKRSST
                     LYTATTYSVSREAKMLSWLSEKLKVPELIMTFQDEQFEFMITKAINAKPISALFLTDQE
                     LLAIYKEVLNLLNSIAIIDCPFISNIDHRLKESKFFIDNQLLDDIDQDDFDAELWGDHK
                     TYLSLWNELNETRVEERLVFSHGDITDSNIFIDKFNEIYFLDLGRAGLADEFVDISFVE
                     RCLREDASEETAKIFLKHLKNDRPDKRNYFLKLDELN"
                     /name="APH(3')-VI family aminoglycoside
                     O-phosphotransferase"
     gene            complement(1599..1883)
                     /locus_tag="LJU41_RS22280"
                     /old_locus_tag="LJU41_22280"
     CDS             complement(1599..1883)
                     /locus_tag="LJU41_RS22280"
                     /old_locus_tag="LJU41_22280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein C"
                     /protein_id="WP_000238497.1"
                     /translation="MVKGSNKAADRLAKLEEQRARINAEIQRVRAREQQQERKNETRRK
                     VLVGAMILAKVNSSEWPEDRLMAAMDAYLERDHDRALFGLPPRQKDEPG"
                     /name="mobilization protein C"
     gene            2082..>2723
                     /locus_tag="LJU41_RS22285"
                     /old_locus_tag="LJU41_22285"
                     /pseudo=""
     CDS             2082..>2723
                     /locus_tag="LJU41_RS22285"
                     /old_locus_tag="LJU41_22285"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /name="MobA/MobL family protein"
     gene            2830..3243
                     /locus_tag="LJU41_RS22290"
                     /old_locus_tag="LJU41_22290"
     CDS             2830..3243
                     /locus_tag="LJU41_RS22290"
                     /old_locus_tag="LJU41_22290"
                     /inference="COORDINATES: protein motif:HMM:NF036929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncQ-type mobilization protein MobB"
                     /protein_id="WP_010890157.1"
                     /translation="MNAIDRVKKSRGINELAEQIEPLAQSMATLADEARQVMSQTQQAS
                     EAQAAEWLKAQRQTGAAWVELAKELREVAAEVSSAAQSARSASRGWHWKLWLTVMLASM
                     MPTVVLLIASLLLLDLTPLTTEDGSIWLRLVAR"
                     /name="IncQ-type mobilization protein MobB"
     gene            3342..4211
                     /locus_tag="LJU41_RS22295"
                     /old_locus_tag="LJU41_22295"
     CDS             3342..4211
                     /locus_tag="LJU41_RS22295"
                     /old_locus_tag="LJU41_22295"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="WP_229382459.1"
                     /translation="MNREWSAAEVLQNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDL
                     SEFDLDDMKAEGREPALVVETSPKNYQAWVKVADAAGGELRGQIARTLASEYDADPASA
                     DSRHYGRLAGFTNRKDKHTTRAGYQPWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQ
                     EKARRLASLELPERQLSRHRRTALDEYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRS
                     AEEIGKAMAEASPALAERKPGHEADYIERTVSKVMGLPSVQLARAELARAPAPRQRGMD
                     RGGPDFSM"
                     /name="DNA-primase RepB domain-containing protein"
     gene            4272..4484
                     /locus_tag="LJU41_RS22300"
                     /old_locus_tag="LJU41_22300"
     CDS             4272..4484
                     /locus_tag="LJU41_RS22300"
                     /old_locus_tag="LJU41_22300"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000455501.1"
                     /translation="MEYEKSASGSVYLIKSDKGYWLPGGFGYTSNKAEAGRFSVADMAS
                     LNLDGCTLSLFREDKPFGPGKFLGD"
                     /name="hypothetical protein"
     gene            4486..4692
                     /gene="urfF"
                     /locus_tag="LJU41_RS22305"
                     /old_locus_tag="LJU41_22305"
     CDS             4486..4692
                     /gene="urfF"
                     /locus_tag="LJU41_RS22305"
                     /old_locus_tag="LJU41_22305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid repression protein F"
                     /protein_id="WP_007857788.1"
                     /translation="MKDQKDKQTGDLLASPDAVRQARYAERMKAKGMRQRKFWLTDDEY
                     EALRECLEELRAAQGGGSDPASA"
                     /name="urfF"
     gene            4722..5561
                     /locus_tag="LJU41_RS22310"
                     /old_locus_tag="LJU41_22310"
     CDS             4722..5561
                     /locus_tag="LJU41_RS22310"
                     /old_locus_tag="LJU41_22310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="WP_000190708.1"
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
                     /name="helicase RepA family protein"
     gene            5548..6399
                     /gene="repC"
                     /locus_tag="LJU41_RS22315"
                     /old_locus_tag="LJU41_22315"
     CDS             5548..6399
                     /gene="repC"
                     /locus_tag="LJU41_RS22315"
                     /old_locus_tag="LJU41_22315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein C, IncQ-type"
                     /protein_id="WP_000240536.1"
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
                     /name="repC"
     gene            6846..7985
                     /locus_tag="LJU41_RS22320"
                     /old_locus_tag="LJU41_22320"
     CDS             6846..7985
                     /locus_tag="LJU41_RS22320"
                     /old_locus_tag="LJU41_22320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711650.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="WP_265334573.1"
                     /translation="MMLIVADAINLGLTKMAESCPGTTYAKLSWLQAWHIRDETYSTAL
                     AELVNAQFRQPFAGNWGDGTTSSSDGQNFRTGSKAESTGHINPKYGSSPGRTFYTHISD
                     QYAPFSAKVVNVGIRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFGLMHLLGFRFA
                     PRIRDLGETKLFIPKGDAAYDALKPMISSDRLNIKQIRAHWDEILRLATSIKQGTVTAS
                     LMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNALAR
                     AVFFYRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERATSALRGNGTALDDTLL
                     QYLSPLGWEHINLTGDYLWRSSAKVGAGKFRPLRPLPPA"
                     /name="Tn3 family transposase"
     gene            8064..9068
                     /locus_tag="LJU41_RS22325"
                     /old_locus_tag="LJU41_22325"
     CDS             8064..9068
                     /locus_tag="LJU41_RS22325"
                     /old_locus_tag="LJU41_22325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229637.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS5075 family transposase"
                     /protein_id="WP_000427614.1"
                     /translation="MENIALIGIDLGKNSFHIHCQDHRGKAVYRKKFTRPKLIEFLATC
                     PATTIAMEACGGSHFMARKLAELGHFPKLISPQFVRPFVKSNKNDFVDAEAICEAASRP
                     SMRFVQPRTESQQAMRALHRVRESLVQDKVKTTNQMHAFLLEFGISVPRGAAVISRLST
                     LLEDSSLPLYLSQLLLKLQQHYHYLVEQIKDLESQLKRKLDEDEVGQRLLSIPCVGTLT
                     ASTISTEIGDGKQYASSRDFAAATGLVPRQYSTGGRTTLLGISKRGNKKIRTLLVQCAR
                     VFIQKLEHQSGKLADWVRELLCRKSNFVVTCALANKLARIAWALTARQQTYEA"
                     /name="IS110-like element IS5075 family transposase"
     ARG             complement(12..787)
                     /name="aph(3')-VIa"
                     /gene="aph(3')-VIa"
                     /gene_name="aminoglycoside O-phosphotransferase
                     APH(3')-VIa"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="98.46"
                     /coverage="100.0"
     Typing          1838..1993
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.28e-76"
     Typing          4740..5561
                     /name="IncQ1"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          2083..4208
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cttggaatca attcaattca tcaagtttta aaaaataatt ccttttgtca ggtctatcat
       61 tttttaaatg ctttaaaaat attttagcag tttcctccga tgcatcctct cttaggcaac
      121 gttcaacaaa ggatatatct acaaactcat ctgctaaccc agcacggcca agatctaaaa
      181 aataaatttc attgaattta tctataaaaa tattactatc cgtgatatcg ccatgagaaa
      241 aaaccaatct ttcttcaaca cgagtctcat ttaactcatt ccatagactt aggtaagttt
      301 tatggtctcc ccataattca gcgtcaaaat catcttgatc tatatcatca aggagttggt
      361 tatcaataaa aaattttgac tcttttaacc gatgatcaat gtttgaaata aatggacaat
      421 caataatagc aattgaattt aacagattga gtacctcctt atagatagca agcaattctt
      481 ggtctgttaa aaaaagcgct gaaattggtt ttgcattgat cgctttagtg atcatgaatt
      541 caaactgctc atcctgaaaa gtcatgatga gttcaggcac ctttaacttc tcagagagcc
      601 aactcaacat tttcgcttca cgagaaacac tgtatgtggt cgctgtatat aaagtgctag
      661 atcgcttaag aaaaaaagtt tcattatttc gattaaaaga ataaacatcc gatggcgact
      721 gaccaatttt atttggctct aaaacgctgt ttccaataaa ttgttgaata atattgggca
      781 attccatcaa gctttccttt tattcagtat taaaaacccc gcaaatgcga ggcctagtaa
      841 atagatgatc ttaatttggt tcactgtagc aaaaatatgg cttattgcct agaagaactt
      901 gcaaaattat tgtcccagcc gacaaaatct agtttagttc agacttgtat taataataaa
      961 tatatgaaac attactactt tatttattat taagccagca tataccgtgg gcacaatatc
     1021 cactacgtta ctattgaccc aaggcatact ggtaagggga aaccccttaa ccccaacccc
     1081 tcacaaaaag ctgtgagggg taaaaaacaa ccgcaagata gaggttccgt tgctagacat
     1141 tgccagccag tgcccgtcac tcccgtacta actgtcacga acccctgcaa taactgtcac
     1201 gcccccctgc aataactgtc acgaacccct gcaataactg tcacgccccc aaacctgcaa
     1261 acccagcagg ggcgggggct ggcggggtgt tggaaaaatc catccatgat tatctaagaa
     1321 taatccacta ggcgcggtta tcagcgccct tgtggggcgc tgctgccctt gcccaatatg
     1381 cccggccaga ggccggatag ctggtctatt cgctgcgcta ggctacacac cgccccaccg
     1441 ctgcgcggca gggggaaagg cgggcaaagc ccgctaaacc ccacaccaaa ccccgcagaa
     1501 atacgctgga gcgcttttag ccgctttagc ggcctttccc cctacccgaa gggtgggggc
     1561 gcgtgtgcag ccccgcaggg cctgtctcgg tcgatcattc agcccggctc atccttctgg
     1621 cgtggcggca gaccgaacaa ggcgcggtcg tggtcgcgtt caaggtacgc atccattgcc
     1681 gccatgagcc gatcctccgg ccactcgctg ctgttcacct tggccaaaat catggccccc
     1741 accagcacct tgcgccttgt ttcgttcttg cgctcttgct gctgttccct tgcccgcacc
     1801 cgctgaattt cggcattgat tcgcgctcgt tgttcttcga gcttggccag ccgatccgcc
     1861 gccttgttgc tccccttaac catcttgaca ccccattgtt aatgtgctgt ctcgtaggct
     1921 atcatggagg cacagcggcg gcaatcccga ccctactttg taggggaggg cgcacttacc
     1981 ggtttctctt cgagaaactg gcctaacggc cacccttcgg gcggtgcgct ctccgagggc
     2041 cattgcatgg agccgaaaag caaaagcaac agcgaggcag catggcgatt tatcacctta
     2101 cggcgaaaac cggcagcagg tcgggcggcc aatcggccag ggccaaggcc gactacatcc
     2161 agcgcgaagg caagtatgcc cgcgacatgg atgaagtctt gcacgccgaa tccgggcaca
     2221 tgccggagtt cgtcgagcgg cccgccgact actgggatgc tgccgacctg tatgaacgcg
     2281 ccaatgggcg gctgttcaag gaggtcgaat ttgccctgcc ggtcgagctg accctcgacc
     2341 agcagaaggc gctggcgtcc gagttcgccc agcacctgac cggtgccgag cgcctgccgt
     2401 atacgctggc catccatgcc ggtggcggcg agaacccgca ctgccacctg atgatctccg
     2461 agcggatcaa tgacggcatc gagcggcccg ccgctcagtg gttcaagcgg tacaacggca
     2521 agaccccgga gaagggcggg gcacagaaga ccgaagcgct caagcccaag gcatggcttg
     2581 agcagacccg cgaggcatgg gccgaccatg ccaaccgggc attagagcgg gctggccacg
     2641 acgcccgcat tgaccacaga acacttgagg cgcagggcat cgagcgcctg cccggtgttc
     2701 acctggggcc gaacgtggtg gagatggaag gccggggcat ccgcaccgac cgggcagacg
     2761 tggccctgaa catcgacacc gccaacgccc agatcatcga cttacaggaa taccgggagg
     2821 caatagacca tgaacgcaat cgacagagtg aagaaatcca gaggcatcaa cgagttagcg
     2881 gagcagatcg aaccgctggc ccagagcatg gcgacactgg ccgacgaagc ccggcaggtc
     2941 atgagccaga cccagcaggc cagcgaggcg caggcggcgg agtggctgaa agcccagcgc
     3001 cagacagggg cggcatgggt ggagctggcc aaagagttgc gggaggtagc cgccgaggtg
     3061 agcagcgccg cgcagagcgc ccggagcgcg tcgcgggggt ggcactggaa gctatggcta
     3121 accgtgatgc tggcttccat gatgcctacg gtggtgctgc tgatcgcatc gttgctcttg
     3181 ctcgacctga cgccactgac aaccgaggac ggctcgatct ggctgcgctt ggtggcccga
     3241 tgaagaacga caggactttg caggccatag gccgacagct caaggccatg ggctgtgagc
     3301 gcttcgatat cggcgtcagg gacgccacca ccggccagat gatgaaccgg gaatggtcag
     3361 ccgccgaagt gctccagaac acgccatggc tcaagcggat gaatgcccag ggcaatgacg
     3421 tgtatatcag gcccgccgag caggagcggc atggtctggt gctggtggac gacctcagcg
     3481 agtttgacct ggatgacatg aaagccgagg gccgggagcc tgccctggta gtggaaacca
     3541 gcccgaagaa ctatcaggca tgggtcaagg tggccgacgc cgcaggcggt gaacttcggg
     3601 ggcagattgc ccggacgctg gccagcgagt acgacgccga cccggccagc gccgacagcc
     3661 gccactatgg ccgcttggcg ggcttcacca accgcaagga caagcacacc acccgcgccg
     3721 gttatcagcc gtgggtgctg ctgcgtgaat ccaagggcaa gaccgccacc gctggcccgg
     3781 cgctggtgca gcaggctggc cagcagatcg agcaggccca gcggcagcag gagaaggccc
     3841 gcaggctggc cagcctcgaa ctgcccgagc ggcagcttag ccgccaccgg cgcacggcgc
     3901 tggacgagta ccgcagcgag atggccgggc tggtcaagcg cttcggtgat gacctcagca
     3961 agtgcgactt tatcgccgcg cagaagctgg ccagccgggg ccgcagtgcc gaggaaatcg
     4021 gcaaggccat ggccgaggcc agcccagcgc tggcagagcg caagcccggc cacgaagcgg
     4081 attacatcga gcgcaccgtc agcaaggtca tgggtctgcc cagcgtccag cttgcgcggg
     4141 ccgagctggc acgggcaccg gcaccccgcc agcgaggcat ggacaggggc gggccagatt
     4201 tcagcatgta gtgctttcgt tggtactcac gcctgttata ctatgagtac tcacgcacag
     4261 aagggggttt tatggaatac gaaaaaagcg cttcagggtc ggtctacctg atcaaaagtg
     4321 acaagggcta ttggttgccc ggtggctttg gttatacgtc aaacaaggcc gaggctggcc
     4381 gcttttcagt cgctgatatg gccagcctta accttgacgg ctgcaccttg tccttgttcc
     4441 gcgaagacaa gcctttcggc cccggcaagt ttctcggtga ctgatatgaa agaccaaaag
     4501 gacaagcaga ccggcgacct gctggccagc cctgacgctg tacgccaagc gcgatatgcc
     4561 gagcgcatga aggccaaagg gatgcgtcag cgcaagttct ggctgaccga cgacgaatac
     4621 gaggcgctgc gcgagtgcct ggaagaactc agagcggcgc agggcggggg tagtgacccc
     4681 gccagcgcct aaccaccaac tgcctgcaaa ggaggcaatc aatggctacc cataagccta
     4741 tcaatattct ggaggcgttc gcagcagcgc cgccaccgct ggactacgtt ttgcccaaca
     4801 tggtggccgg tacggtcggg gcgctggtgt cgcccggtgg tgccggtaaa tccatgctgg
     4861 ccctgcaact ggccgcacag attgcaggcg ggccggatct gctggaggtg ggcgaactgc
     4921 ccaccggccc ggtgatctac ctgcccgccg aagacccgcc caccgccatt catcaccgcc
     4981 tgcacgccct tggggcgcac ctcagcgccg aggaacggca agccgtggct gacggcctgc
     5041 tgatccagcc gctgatcggc agcctgccca acatcatggc cccggagtgg ttcgacggcc
     5101 tcaagcgcgc cgccgagggc cgccgcctga tggtgctgga cacgctgcgc cggttccaca
     5161 tcgaggaaga aaacgccagc ggccccatgg cccaggtcat cggtcgcatg gaggccatcg
     5221 ccgccgatac cgggtgctct atcgtgttcc tgcaccatgc cagcaagggc gcggccatga
     5281 tgggcgcagg cgaccagcag caggccagcc ggggcagctc ggtactggtc gataacatcc
     5341 gctggcagtc ctacctgtcg agcatgacca gcgccgaggc cgaggaatgg ggtgtggacg
     5401 acgaccagcg ccggttcttc gtccgcttcg gtgtgagcaa ggccaactat ggcgcaccgt
     5461 tcgctgatcg gtggttcagg cggcatgacg gcggggtgct caagcccgcc gtgctggaga
     5521 ggcagcgcaa gagcaagggg gtgccccgtg gtgaagccta agaacaagca cagcctcagc
     5581 cacgtccggc acgacccggc gcactgtctg gcccccggcc tgttccgtgc cctcaagcgg
     5641 ggcgagcgca agcgcagcaa gctggacgtg acgtatgact acggcgacgg caagcggatc
     5701 gagttcagcg gcccggagcc gctgggcgct gatgatctgc gcatcctgca agggctggtg
     5761 gccatggctg ggcctaatgg cctagtgctt ggcccggaac ccaagaccga aggcggacgg
     5821 cagctccggc tgttcctgga acccaagtgg gaggccgtca ccgctgatgc catggtggtc
     5881 aaaggtagct atcgggcgct ggcaaaggaa atcggggcag aggtcgatag tggtggggcg
     5941 ctcaagcaca tacaggactg catcgagcgc ctttggaagg tatccatcat cgcccagaat
     6001 ggccgcaagc ggcaggggtt tcggctgctg tcggagtacg ccagcgacga ggcggacggg
     6061 cgcctgtacg tggccctgaa ccccttgatc gcgcaggccg tcatgggtgg cggccagcat
     6121 gtgcgcatca gcatggacga ggtgcgggcg ctggacagcg aaaccgcccg cctgctgcac
     6181 cagcggctgt gtggctggat cgaccccggc aaaaccggca aggcttccat agataccttg
     6241 tgcggctatg tctggccgtc agaggccagt ggttcgacca tgcgcaagcg ccgccagcgg
     6301 gtgcgcgagg cgttgccgga gctggtcgcg ctgggctgga cggtaaccga gttcgcggcg
     6361 ggcaagtacg acatcacccg gcccaaggcg gcaggctgac ccccctgttt aacaatcacg
     6421 cccccccggt aactgtcacg ccccccccgg taactgtcac gaaccccccc ggtaactgtc
     6481 acgcccccaa acctgcaaac ccaggaaggg tgggagttgg cgtggtgttg gaaaaatcca
     6541 tccatgatta tccaagaata atccactagg cgcggttaac agcgccctag tggggcactg
     6601 tttgcccttg cctaaaatgc cggccagagg ccggataatt gctctatccg ctgcgctagg
     6661 ctgcacacgc ccccccaccg ctgcgcggca ggggggaaag gcgggcaaag cccgctaaac
     6721 cccacaccaa attctgcggg aatacgctgg agcgatttta gccgctttag cggccttttc
     6781 cctacccgaa gggtgggggc ggtgtgcagc cccgaagggg ccatttagcg gccatttcag
     6841 cggctgtgat gctgatagtg gccgacgcga tcaacctggg tctgaccaaa atggccgagt
     6901 cctgccctgg caccacctac gccaagctgt cttggctgca agcctggcac atccgcgatg
     6961 aaacctattc gacggcgctg gccgagctgg tgaatgcgca gtttcggcaa cccttcgccg
     7021 gcaactgggg tgacggcacc acgtcatcgt cggacggcca gaacttcaga accggcagca
     7081 aagcagaaag cactggtcat atcaacccga agtatggaag cagtccagga cggactttct
     7141 acacccatat ctccgaccag tacgcgccct tcagtgccaa ggtggtcaac gtgggcattc
     7201 gtgattcaac ttacgtgctt gatggcctgc tgtaccacga gtcggacttg cgcatcgagg
     7261 aacactacac cgacacggca ggcttcaccg atcacgtgtt tggcttgatg catttgctgg
     7321 gatttcgctt cgcgccgcgt atccgtgact tgggcgaaac caagctattc atccccaagg
     7381 gcgatgccgc ctatgacgcg ctcaagccga tgattagcag cgacaggctg aacatcaagc
     7441 aaatacgcgc ccattgggat gaaattctgc ggctggccac ctccatcaag caaggcacgg
     7501 taacggcttc gctgatgctg cgcaaactcg gcagctaccc gcgccagaac ggcttggccg
     7561 tggcgttgcg cgagctgggg cgcatcgagc gcacgctgtt cattttggat tggctgcaaa
     7621 gcgtggagct gcgccgccgc gtccatgcgg ggctgaataa gggcgaggcg cgcaacgcgc
     7681 tggccagggc ggtcttcttc taccgattgg gtgaaatccg cgaccgcagt tttgagcagc
     7741 agcgctaccg ggccagcggc ctcaatctgg tgacggcggc catcgtgttg tggaacacgg
     7801 tatatctgga gcgtgccacc agtgctttgc gtggcaacgg cacggcgctg gacgacacat
     7861 tgttgcaata tctgtcgccg ctggggtggg agcacatcaa cctgaccggc gattacctat
     7921 ggcgcagcag cgccaaggtc ggtgcgggga agtttaggcc attgcgaccg ctgccaccgg
     7981 cttagcgtgc tttatttaat gagatggtca ctccctcctt cccagtacta tgctgaggac
     8041 aggctttcat tcggagaacc atcatggaaa acattgcgct tattggtatc gatctgggta
     8101 agaactcttt ccatattcat tgtcaggatc atcgtgggaa ggccgtttac cgtaaaaaat
     8161 tcacccgacc aaagctaatc gaatttctgg cgacatgccc agcaacaacc atcgcgatgg
     8221 aagcctgtgg cggttctcac tttatggcac gcaagctggc agagttaggg cattttccaa
     8281 agctgatatc accgcaattt gtccgcccat tcgttaaaag caacaaaaat gacttcgttg
     8341 atgctgaagc tatctgtgaa gcagcatcac gtccatctat gcgtttcgtg cagcccagaa
     8401 ccgaatctca gcaggcaatg cgagctctgc atcgtgtccg tgaatccctg gttcaggata
     8461 aggtgaaaac aactaatcag atgcatgctt ttctgctgga atttggtatc agcgttccgc
     8521 gaggtgctgc cgttattagt cgactgagta cccttcttga ggacagtagt ttgcctcttt
     8581 atctcagcca gttactgctg aaattacaac agcattatca ctatcttgtt gagcagatta
     8641 aagatctgga atctcagttg aaacgaaagt tggacgaaga tgaggttgga cagcgcttgc
     8701 tgagtattcc ctgcgttgga acgctgactg ccagtactat ttcaactgag attggcgacg
     8761 ggaagcagta cgccagcagc cgtgactttg cggcggcaac agggctggta ccccgacagt
     8821 acagcacggg aggtcggacg acattgttag ggattagcaa gcggggcaac aaaaagatcc
     8881 gaactttgtt ggttcagtgt gccagggtat tcatacaaaa actggaacac cagtctggca
     8941 agttggccga ctgggtcagg gagttgttgt gtcggaaaag caactttgtc gtcacctgtg
     9001 ctctggcaaa caagctggcc agaatagcct gggcactgac ggcgcgacag caaacttacg
     9061 aagcataaag gcagaaatac accagtttaa acaatcattc atctggtttt gcgaatactg
     9121 atattgatga tacaaacggc ccaccggcct gttgaggaac ctgtaaaacg gaaaggctcg
     9181 ctgaagccgt atattttctg gaggttcatc aggcgcggaa ctcatcgagg cgcgggaata
     9241 aaatcccatt cagacgccgg atagattcaa gcaagccaac ttatcgtcaa aatcggtgtt
     9301 gcaaaaacgg gagtgaccat agattccgtt ttctgagacg acccctgcat ctacggtcca
     9361 aagaatcgac gtaaaatgac aattaagtat ttttagaaaa tg
//
