LOCUS       NZ_CP085761             3530 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 54569CZ plasmid p54569CZ_ColpVC,
            complete sequence.
ACCESSION   NZ_CP085761
VERSION     NZ_CP085761.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377422
            Assembly: GCF_020695605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695605.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:44:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,550
            Genes (coding)                    :: 4,432
            CDSs (with protein)               :: 4,432
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 43 of 118
            Pseudo Genes (incomplete)         :: 75 of 118
            Pseudo Genes (internal stop)      :: 18 of 118
            Pseudo Genes (multiple problems)  :: 17 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085761.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="54569CZ"
                     /isolation_source="wound abdomin"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p54569CZ_ColpVC"
                     /geo_loc_name="Czech Republic: Hradec Kralove Region"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-11"
                     /collected_by="Fakultni nemocnice Hradec Kralove"
     gene            join(3462..3530,1..891)
                     /locus_tag="LJU41_RS22265"
                     /old_locus_tag="LJU41_22265"
                     /pseudo=""
     CDS             join(3462..3530,1..891)
                     /locus_tag="LJU41_RS22265"
                     /old_locus_tag="LJU41_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245711.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /name="protein rep"
     gene            1682..2671
                     /locus_tag="LJU41_RS22270"
                     /old_locus_tag="LJU41_22270"
     CDS             1682..2671
                     /locus_tag="LJU41_RS22270"
                     /old_locus_tag="LJU41_22270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_032219167.1"
                     /translation="MQNPKLQNLTDYSPADAPWDAHRSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNAGFQRLKDRKEFSPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMLSGRDYIKHERWVDLWRGCLRVDYDPNVDVRAVKPRKPKDG
                     ESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGALKDVLK
                     LDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESE"
                     /name="protein rep"
     Typing          complement(618..809)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
     Typing          complement(2398..2589)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="97.409"
                     /coverage="100.0"
                     /evalue="6.18e-88"
ORIGIN
        1 cctgcttgcg gctgaatatg agcgctacgg ggcgcgtatg gcctcctgtg gcggtttgct
       61 gcggttcggg tggtctaccc tcaaagaaac ggagaaacgc gcctgaggct gcgtgaggcg
      121 catttctgcc gtgtccggca ctgtcctgtc tgccagtggg gcgctccctg atgtggcaag
      181 cccgtttcta ccagtccctg ccgcgtatcg tggcggatta tcccgatgcc cgctggatgt
      241 tcctgaccct gactgtgcgt aactgtgcga tcggggagct gggcgagacg ctgaaccgga
      301 tgaacgcagg ttttccagcg actgaaggat cgcaaggagt tcagtccagt gcagggctgg
      361 atacgtacca cggaagtcac ccgcgggagc gacggcagcg cacacccgca ctttcacacc
      421 ctgatgatgg tgccgccatc gatgttgtct gggcgtgatt acatcaagca tgagcgctgg
      481 gttgatctgt ggcgtgggtg tcttcgggtg gattacgatc cgaatgtcga tgtccgggca
      541 gtaaaacccc ggaagccgaa ggacggcgaa tcgctggcct gcgcgaccgc cgaactggtg
      601 cgcggcgcag tggccgaaac cctgaaatat tccacaaagc cggctgacat ggtggccgat
      661 cctgaatggt tcctcgaact gacccgccag acgcacaagc gccgatttgt ggcgaccggc
      721 ggcgcactca aagacgtact gaagctcgat caggaaaccg acgcagacat ggtgatcggg
      781 gatgatattt ccgagggcga cgatgacggt tcccgcatcg cgtttgagtg gaaaactgag
      841 gtcagaaagt accgtcgctc accgacgaaa gataaaaccg aatcagagta acggggtgtc
      901 ggggctgcgc cctgaccatg aggactttgg gcatccgtca ccttggtggc ggtgtaccaa
      961 agtcacatct tgtccgatag attttactcc gatgcaggat agggctaccg ccgcaaagtg
     1021 acgccaatcg cttactgagt tcggaatccg aacttagggt tttgcgatcc gtaatacgga
     1081 tttttttaac gggacaagat gtgtttttgg agtaccgcca tcacgatgcc ggtaccaaaa
     1141 acgatccgtc agggctgcgc cccgacaccc tgccaacacc tcacgccgcc agattgaaat
     1201 ctgtcagcgt tcgccgttac tggaatcgtc aaacgctaaa tcgtggggtc ccgctggctt
     1261 gccagtgggt tccgatttag tgattgacga ctgcacccat aaaacactgc cggcaggtgg
     1321 ttgtgatcac gcagtgagcg caaccacctg ataagcgttt ttgggttttc tggcggggcg
     1381 gatgtacggg gaaattaggt gggaaattcg cgcgcgttcc ttgtatttat acttaaggga
     1441 taactctggg attggaattc ggcctcagcc cttgtgcggc aaggcctgag aggtgatttg
     1501 cttggaaatg ggtttggaag tactctgttt ggagtggtcg cgttaaaagc gacccccgca
     1561 acgagggcaa tcgttcgggg gcgtgaccac ctgtaggaat tctattttgc gacagacaac
     1621 acaggtgata cggcaatgat aacacgtcaa atgagccatc gcccagtatg gggcggtgtg
     1681 catgcaaaat cctaaactcc agaacctcac ggattattcc cccgcagatg cgccctggga
     1741 cgctcacaga agcgtttctg acgacgttgg agggatttac ctgcttgcgg ctgaatatga
     1801 gcgctacggg gcgcgtatgg cctcctgtgg cggtttgctg cggttcgggt ggtctaccct
     1861 caaagaaacc ggagaaacgc gcctgaggct gcgtgaggcg catttctgcc gtgtccggca
     1921 ctgtcctgtc tgccagtgga ggcgctccct gatgtggcaa gcccgtttct accagtccct
     1981 gccgcgtatc gtggcggatt atcccgatgc ccgctggatg ttcctgaccc tgactgtgcg
     2041 taactgtgcg atcggggagc tgggcgagac gctgaaccgg atgaacgcag gtttccagcg
     2101 actgaaggat cgcaaggagt tcagtccagt gcagggctgg atacgtacca cggaagtcac
     2161 ccgcgggagc gacggcagcg cacacccgca ctttcacacc ctgatgatgg tgccgccatc
     2221 gatgttgtct gggcgtgatt acatcaagca tgagcgctgg gttgatctgt ggcgtgggtg
     2281 tcttcgggtg gattacgatc cgaatgtcga tgtccgggca gtaaaacccc ggaagccgaa
     2341 ggacggcgaa tcgctggcct gcgcgaccgc cgaactggtg cgcggcgcag tggccgaaac
     2401 cctgaaatat tccacaaagc cggctgacat ggtggccgat cctgaatggt tcctcgaact
     2461 gacccgccag acgcacaagc gccgatttgt ggcgaccggc ggcgcactca aagacgtact
     2521 gaagctcgat caggaaaccg acgcagacat ggtgatcggg gatgatattt ccgagggcga
     2581 cgatgacggt tcccgcatcg cgtttgagtg gaaaactgag gtcagaaagt accgtcgctc
     2641 accgacgaaa gataaaaccg aatcagagta acggggtgtc ggggctgcgc cctgaccatg
     2701 aggactttgg gcatccgtca ccttggtggc ggtgtaccaa agtcacatct tgtccgatag
     2761 attttactcc gatgcaggat agggctaccg ccgcaaagtg acgccaatcg cttactgagt
     2821 tcggaatccg aacttagggt tttgcgatcc gtaatacgga tttttttaac gggacaagat
     2881 gtgtttttgg agtaccgcca tcacgatgcc ggtaccaaaa acgatccgtc agggctgcgc
     2941 cccgacaccc tgccaacacc tcacgccgcc agattgaaat ctgtcagcgt tcgccgttac
     3001 tggaatcgtc aaacgctaaa tcgtggggtc ccgctggctt gccagtgggt tccgatttag
     3061 tgattgacga ctgcacccat aaaacactgc cggcaggtgg ttgtgatcac gcagtgagcg
     3121 caaccacctg ataagcgttt ttgggttttc tggcggggcg gatgtacggg gaaattaggt
     3181 gggaaattcg cgcgcgttcc ttgtatttat acttaaggga taactctggg attggaattc
     3241 ggcctcagcc cttgtgcggc aaggcctgag aggtgatttg cttggaaatg ggtttggaag
     3301 tactctgttt ggagtggtcg cgttaaaagc gacccccgca acgagggcaa tcgttcgggg
     3361 gcgtgaccac ctgtaggaat tctattttgc gacagacaac acaggtgata cggcaatgat
     3421 aacacgtcaa atgagccatc gcccagtatg gggcggtgtg catgcaaaat cctaactcca
     3481 gaacctcacg gattattccc ccgcagatgc gccctgggga cgctcacaga
//
