LOCUS       NZ_CP085760             2317 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 54569CZ plasmid p54569CZ_Col4401,
            complete sequence.
ACCESSION   NZ_CP085760
VERSION     NZ_CP085760.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377422
            Assembly: GCF_020695605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695605.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:44:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,550
            Genes (coding)                    :: 4,432
            CDSs (with protein)               :: 4,432
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 43 of 118
            Pseudo Genes (incomplete)         :: 75 of 118
            Pseudo Genes (internal stop)      :: 18 of 118
            Pseudo Genes (multiple problems)  :: 17 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085760.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="54569CZ"
                     /isolation_source="wound abdomin"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p54569CZ_Col4401"
                     /geo_loc_name="Czech Republic: Hradec Kralove Region"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-11"
                     /collected_by="Fakultni nemocnice Hradec Kralove"
     gene            join(2178..2317,1..397)
                     /locus_tag="LJU41_RS22255"
                     /old_locus_tag="LJU41_22255"
     CDS             join(2178..2317,1..397)
                     /locus_tag="LJU41_RS22255"
                     /old_locus_tag="LJU41_22255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(778..891)
                     /locus_tag="LJU41_RS23405"
     CDS             complement(778..891)
                     /locus_tag="LJU41_RS23405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     gene            1653..1889
                     /locus_tag="LJU41_RS22260"
                     /old_locus_tag="LJU41_22260"
     CDS             1653..1889
                     /locus_tag="LJU41_RS22260"
                     /old_locus_tag="LJU41_22260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     Typing          1245..1420
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.358"
                     /coverage="82.0"
                     /evalue="3.53e-41"
     Typing          complement(779..1262)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.213"
                     /coverage="93.0"
                     /evalue="0.0"
ORIGIN
        1 ttattataag tggtttgtaa actcttccga gattaattat gaccttcgtt ctgtttctgc
       61 agaaaatagt tcactggatg ttagtagggt gatgctgcaa tttgctgaaa aagtgaaaga
      121 ttatgatttt agtaaggttt atcttagtta cagagggaaa gataagtttt acttaaaagg
      181 agagtatttt aaaactcttg ggcaggaata tggtattcaa aaccccgtat atacattaag
      241 aacaattccg gaaaacgtgt atatgttaaa tggagagaga gcttatagtg tttgggaggg
      301 tgggcttctg ggtgtaatgg gaaaacagat ggaagatctt agtgatttct ctaaggcttg
      361 gtacttagat gatttcatta aaagcatgag cgattaattt gaagcgatca gtatacattg
      421 acgattagct cacgcaaatc gctcattggt cactggggtg tttttactcg tagatgcttt
      481 agatgtgtga cctgctcccc gtggattaca aggccgtcat ctgataatgc ccctaatggg
      541 tcagatctct aggatagtag caatacagca aggaacttct tgagatcctt tctgttgcgt
      601 cgtaatctct tgctctgtaa acgaaaaaac cgcccagcaa ggcggttttc tcgaaggttc
      661 tcagagcagc aactctttga accgaggtaa ctggctagtt agaggagcgc agtcaccaaa
      721 actgttcgtt tagtttagcc ttaactggcg catgacttca agactatctc ctctaaatca
      781 gttaccagtg gctgctgcca gtggcgcttt tgcatgtctt accgggttgg actcaagacg
      841 atagttaccg gataaggcgc agcagtcgga ctgaacgggg ggttcgtgca tacagtccag
      901 cttggagcga actgcctacc cggaactgag tgtcaggcgt ggaatgagat aaacgcggcc
      961 ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc acgagggagc
     1021 caccaggggg aaacgcctgg gatctttaag tcctgtcggg tttcgccgcc actgatttga
     1081 gcgtcagatt ttttgatgct tgtcaggggg gcggagccta tggaaaaacg gcagcgccgc
     1141 atcctttttc gggcccttct tgacctcagc ttcatttcgt tacctcgtca ttcgtgagca
     1201 ggtaacgccc gccgcagtct cacggcagga gcgtagcgac cgagtgagcg aggaggcgtc
     1261 atttcaccat catcagcatt ttgcacttac gcaccgcagc cgcatttttc ccctgccaca
     1321 tgaagcactt ccctgagcca ccacgcccaa ctaacatatt aagccagtat acactccgct
     1381 agcgctacgt gactggtccg gcgctgcgcc cggaacccgc aaattcagaa actgaaagcg
     1441 ggacaagatg tgtttttgga gtaccgccat catgatgccg gcaccaaaac gatatctcag
     1501 ggctgcgccc cgataccccg ccaacgcttc acggcaccgg attaaaatcc gttcccgttc
     1561 accgtttctc agggccgttc ccggctgcag gttcttcatg aggttcgccg tgtctggcag
     1621 actgtgcaga gttattctga ccggagaacg ccatgaaacg actgataaac ctgacgcctg
     1681 ccgagaaaag atttctcgac gatgctgttg ccgccgctga gagagcgtca ggcaagaagc
     1741 tgaatcagcc caacaggcac attgtcctta acagggctcg tgcacagatc gaatcgcagc
     1801 gccaggcaga acggcagcga tctgcgcgtg aggaggagcg gcagcaggcg gaatttacct
     1861 ggtcgcgtcc gcgggcgccg cgtcgctgat tgccactttc gtcggttttg tggggagttc
     1921 agggcgaaac agcgtggtat tcactccggt gcatagtcat tcgccaccgt aaaactcacc
     1981 agagcgattc cgggcggttt tctgggggta agacgtgctc ttttgtggct gaaggcgcag
     2041 aaacgccctg aggccgtttt cgcggtgcgc gtaagaagac gttatggtaa atcgatgctt
     2101 aattccttgc taacttatgg caccttagct taaaaatctc agggtaaact ttagagcagt
     2161 tgagatggat tatagaaatg aaaaaagtat atacagcaat catattaata gtactcttat
     2221 gtggtggtgt tttatctgcc aactacattt ttcttcaaag gcacatgaat gaggttctga
     2281 aagaagatcc aaggaatgat ggtattagcg tttgggt
//
