LOCUS       NZ_CP085758             1716 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 54569CZ plasmid p54569CZ_1, complete
            sequence.
ACCESSION   NZ_CP085758
VERSION     NZ_CP085758.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377422
            Assembly: GCF_020695605.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 247x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695605.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:44:18
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,667
            CDSs (total)                      :: 4,550
            Genes (coding)                    :: 4,432
            CDSs (with protein)               :: 4,432
            Genes (RNA)                       :: 117
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 118
            CDSs (without protein)            :: 118
            Pseudo Genes (ambiguous residues) :: 0 of 118
            Pseudo Genes (frameshifted)       :: 43 of 118
            Pseudo Genes (incomplete)         :: 75 of 118
            Pseudo Genes (internal stop)      :: 18 of 118
            Pseudo Genes (multiple problems)  :: 17 of 118
            Pseudo Genes (short protein)      :: 1 of 118
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085758.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="54569CZ"
                     /isolation_source="wound abdomin"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p54569CZ_1"
                     /geo_loc_name="Czech Republic: Hradec Kralove Region"
                     /lat_lon="50.21 N 15.82 E"
                     /collection_date="2019-11"
                     /collected_by="Fakultni nemocnice Hradec Kralove"
     gene            join(1568..1716,1..154)
                     /locus_tag="LJU41_RS21990"
                     /old_locus_tag="LJU41_21990"
     CDS             join(1568..1716,1..154)
                     /locus_tag="LJU41_RS21990"
                     /old_locus_tag="LJU41_21990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150476.1"
                     /translation="MAGSIRARSLYLYLRDNSGDKKWHQALRTKASGLICVVEGFGSIL
                     LGVVALKATPATTAIVRGCDHLQKFDSKRQPTGDTAMITRQMSHRPAWDGVHAKP"
                     /name="hypothetical protein"
     gene            141..1127
                     /locus_tag="LJU41_RS21995"
                     /old_locus_tag="LJU41_21995"
     CDS             141..1127
                     /locus_tag="LJU41_RS21995"
                     /old_locus_tag="LJU41_21995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_016245711.1"
                     /translation="MQNPKLQNLTDYSPSDAPWDAHKSVSDDVGGIYLLAAEYERYGAR
                     MASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPRIV
                     ADYPDARWMFLTLTVRNCAIGELGETLNRMNMAFQRLKDRKEFRPVQGWIRTTEVTRGS
                     DGSAHPHFHTLMMVPPSMFTRDYVKHDRWVELWRECLRVNYDPNVDVRAVKPRKPKDGE
                     SLASATAELVRGAVAETLKYSTKPADMVADPEWFLELTKQTHKRRFVATGGALKDILKL
                     DQETDADMVIGDDIPEGDDDGSRIAFEWKTESKKYRRSPSKDKAESD"
                     /name="protein rep"
     Typing          complement(854..1042)
                     /name="ColpVC"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.947"
                     /coverage="98.0"
                     /evalue="1.39e-59"
ORIGIN
        1 cttaaaagcg acccccgcaa cgacggccat cgttcggggg tgtgaccacc tgcagaaatt
       61 cgattcgaag cgacaaccaa caggtgatac ggcaatgata acacgtcaaa tgagccatcg
      121 cccagcatgg gacggtgtgc atgcaaaacc ctaaactcca gaacctcacg gattactccc
      181 cctcagacgc gccctgggac gctcacaaaa gcgtttctga cgacgtaggt gggatttacc
      241 ttctcgcagc cgaatatgag cgctacgggg cgcgtatggc ttcctgtggc ggtttactga
      301 ggtttggttg gtctaccctc aaagaaactg gagaaactcg ccttaggcta cgtgaggcgc
      361 atttctgtcg tgtccggcac tgtcctgtct gccagtggag gcgatccctg atgtggcaag
      421 cccgttttta tcagtccctg ccgcgtattg ttgcggatta tccagatgca cgctggatgt
      481 tcctgaccct cacagtgcga aactgcgcga tcggggaatt gggtgagaca cttaaccgaa
      541 tgaacatggc cttccagcga cttaaggatc gcaaagagtt tcgacctgtt cagggctgga
      601 tccgcaccac ggaagttaca cgtggaagtg acggtagtgc gcacccgcac tttcacactc
      661 tgatgatggt tcctccctca atgttcacgc gagattacgt taaacatgac cgctgggtag
      721 agctttggcg agagtgtttg cgtgtgaatt acgatccgaa tgttgatgtt cgagcagtaa
      781 aaccccgcaa gcctaaggac ggagaatcat tggcttctgc aactgcggaa cttgtacggg
      841 gcgcagtggc tgaaaccctg aaatattcca cgaagccggc tgacatggtt gctgatcctg
      901 aatggttcct cgaattgact aagcagacac ataagcgccg atttgtggct accggtggcg
      961 cactcaaaga catactgaaa ctcgatcagg aaactgatgc cgatatggta atcggggatg
     1021 atattcccga aggcgatgat gatggttcac gcattgcttt tgagtggaaa accgaatcca
     1081 aaaagtaccg tcgctcaccg tcaaaagata aagctgaatc agactgacag ggtgtcaggg
     1141 ctgcgccccg acaccctgcc aacacctcac gcagtcagat taaaatctgc cagcgctcgc
     1201 tgtttccgaa atcgtcaaac tctaaatcgt ggggtcccgc tggcttgcca gtgggttccg
     1261 atttagtgat tgacgacatt gctgattgaa aaattggctg aggtggttgg attcacgaag
     1321 tgaggccaac cacctggaag acggttttta attgtctggt accggataca accagaacga
     1381 atggagttgg ttctgtcgtg atgcgaaaaa gtatgggttc gcaaatcaca gactaaatag
     1441 atttacccaa ttccattcga gtgaggttgt tatgtttttt gaggttctta atcatgttga
     1501 tagtgcgttg agcgttgctg tttcgctcct ggcgttgtgg cagtttttgg tggcgggtgg
     1561 gcgttgaatg gctggttcga tccgcgcgcg atccttgtat ttatacttaa gggataactc
     1621 tggcgataaa aaatggcatc aagccttgcg taccaaggct tcggggctta tttgcgttgt
     1681 agaaggtttt ggaagtattc tgttgggagt ggtcgc
//
