LOCUS       NZ_CP085754             6499 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 57816CZ plasmid p57816CZ_Col,
            complete sequence.
ACCESSION   NZ_CP085754
VERSION     NZ_CP085754.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377429
            Assembly: GCF_020695585.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 334x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695585.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:14:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,253
            CDSs (total)                      :: 5,137
            Genes (coding)                    :: 5,017
            CDSs (with protein)               :: 5,017
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 120
            CDSs (without protein)            :: 120
            Pseudo Genes (ambiguous residues) :: 0 of 120
            Pseudo Genes (frameshifted)       :: 52 of 120
            Pseudo Genes (incomplete)         :: 72 of 120
            Pseudo Genes (internal stop)      :: 12 of 120
            Pseudo Genes (multiple problems)  :: 16 of 120
            Pseudo Genes (short protein)      :: 1 of 120
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085754.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="57816CZ"
                     /isolation_source="urine - permanent urinary catheter"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p57816CZ_Col"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2020-06"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            join(6382..6499,1..260)
                     /locus_tag="LJU43_RS24330"
                     /old_locus_tag="LJU43_24330"
     CDS             join(6382..6499,1..260)
                     /locus_tag="LJU43_RS24330"
                     /old_locus_tag="LJU43_24330"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_227789313.1"
                     /translation="MMLDAYRQILSETEPAVRTVMLEFLESVRTSLHFRPEHFQNLFQS
                     QLFYPYQFWSTNHLTIGHFLCEKRLDGGLYIELGARCYGQSVVSGGEEAYMRCNPADTL
                     YQDDLSIPLCEVGCLIIKGSS"
                     /name="hypothetical protein"
     gene            complement(422..582)
                     /locus_tag="LJU43_RS26370"
                     /pseudo=""
     CDS             complement(422..582)
                     /locus_tag="LJU43_RS26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(1778..1999)
                     /locus_tag="LJU43_RS24335"
                     /old_locus_tag="LJU43_24335"
     CDS             complement(1778..1999)
                     /locus_tag="LJU43_RS24335"
                     /old_locus_tag="LJU43_24335"
                     /inference="COORDINATES: protein motif:HMM:NF013937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_265335257.1"
                     /translation="MHQNDAMLFSRKNHHQTGKITSDQTGIRRPQYGQIYPRTDTSPLE
                     RLEHMDLDEAAGCCEHLHDQAEAALRHC"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(2301..2528)
                     /locus_tag="LJU43_RS24340"
                     /old_locus_tag="LJU43_24340"
     CDS             complement(2301..2528)
                     /locus_tag="LJU43_RS24340"
                     /old_locus_tag="LJU43_24340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            3735..3953
                     /locus_tag="LJU43_RS24345"
                     /old_locus_tag="LJU43_24345"
     CDS             3735..3953
                     /locus_tag="LJU43_RS24345"
                     /old_locus_tag="LJU43_24345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(4245..4430)
                     /locus_tag="LJU43_RS24350"
                     /old_locus_tag="LJU43_24350"
     CDS             complement(4245..4430)
                     /locus_tag="LJU43_RS24350"
                     /old_locus_tag="LJU43_24350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(4805..5023)
                     /locus_tag="LJU43_RS24355"
                     /old_locus_tag="LJU43_24355"
                     /pseudo=""
     CDS             complement(4805..5023)
                     /locus_tag="LJU43_RS24355"
                     /old_locus_tag="LJU43_24355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003848985.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          1612..1699
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="96.667"
                     /coverage="100.0"
                     /evalue="6.91e-34"
     Typing          4111..4198
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(434..966)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="87.593"
                     /coverage="88.0"
                     /evalue="5.34e-173"
     Typing          complement(2944..3472)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
     Typing          complement(5460..6004)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="85.353"
                     /coverage="88.0"
                     /evalue="5.5e-153"
ORIGIN
        1 aaaatctttt tcaaagccag ctcttttatc catatcagtt ttggtccact aaccatctaa
       61 ctataggtca ttttttatgt gaaaagaggc ttgatggtgg gctgtatatc gagttaggcg
      121 cgaggtgcta tggtcaaagt gtggtgtcag gcggggagga ggcttatatg agatgtaatc
      181 cggccgatac attataccaa gatgatttaa gtattcctct ttgtgaggtt ggatgtctaa
      241 ttattaaagg atcttcttga gatccttttt ttccgcgcgt aatctcttgc cctgtaaacg
      301 aaaaaaccac cctggcaggt ggtttttcgg aaggttaggt aatcctggca gattccccta
      361 accgttggta acagtcttgt gcgagacatg tcaccaaatt tgtcctttca gtgtagcctc
      421 actaaggccg ccacttcaag aactcttgag acatctctcg cacatcctgt ttgccaatgg
      481 ccgttgccaa tggcgattta gtcgtgtctt tccgaggttg gactcaagtt gatagttacc
      541 ggataaggcg cagcggtcgg actgaacggg gggttcgtgc atacagtcca tcctggagcg
      601 aactgccttc ccggaactga gtgtcaggcg tggaatgaga aaccgcggcc ataacagcgg
      661 agtgacaccg gtaaaccgaa aggcaggaat gcgggggagc acgagggagc caccaggggg
      721 aaacgcctgg tatctttcaa gccgcatcgg gtttcggcca cccatgattt gagcgtcaga
      781 ttctgtgatg cttgtcaggg gggcggagcc tatggaaaaa acggctttgg cgcggcctta
      841 tgctttcttc gttaagtatc ttcctggcat ccccaggaaa tttctgatcc atccgtaagc
      901 ccgtcccgct cgccgcagcc gaacgaccga gcggagcgag tcagtgagcg aggaagcgga
      961 atatattctg tacacatttt ctcctgacag cgttttcttt cactttctgc gcctgtctta
     1021 tgtggcatta atgctatgtg ttactgccat gctacatctt aagccagtat acactccgct
     1081 agtgctccgt gactggtccg gcgctgcgcc cggaaccgcc tgttaccggt tcagcagccg
     1141 tttccggcct gactgcaatt tttttttttc atccctgccc gctaccctgt aaaccttctt
     1201 ctgcgttgcc gttaacagta ggtaagccat gtttggtttt tcttccggag ccctgatgac
     1261 tcttagtgat gatgagcgac acctgcttgt gtccgttgtt tcggtctggc tacaggcgtg
     1321 ccggagggga tgccggaagc catgatgctg gatgcatacc ggcagatttt gtctgaaacc
     1381 gaacaggccg taaggactgg tcatgctcga atttcttgaa tccgttcgca ttcattacct
     1441 ttcgtcctga gcattttctg tatttctgtc catacttaaa acgaggcggc cgtcacggac
     1501 ggtggtcgtc gttccatgat gcttgtatgg acgagtgaga gtaatctaat ctaacttgat
     1561 tgagttccgg gcatgtgccg cccggttttt ttttgctgtt ttcccggtgt ccgtttcggg
     1621 acaggatgtg catttgtaat accgcgcacg cgccggcatc acaaataccc tggtcagggc
     1681 tgcgcccgac aaccccgcca tcgcctcacg gccgcgccac tgaattgcgc acccgttcca
     1741 ccgattttca cggcattttc ctcgcctatc tgtgcgttca gcaatggcgt aaagctgctt
     1801 cggcctgatc gtgcaggtgc tcacagcagc cggcagcctc atccagatcc atatgctcga
     1861 gccgctcaag aggagaagtg tctgtgcgcg gataaatttg gccatattga gggcggcgga
     1921 ttcctgtctg gtcactggtg attttccccg tctggtgatg atttttgcgt gaaaagagca
     1981 tggcgtcatt ctggtgcata gtcatgcatg ccgttaaaat ttaacaggcg cgtttctgac
     2041 gggttccggg gtggtttgtt gtggtttttg tcatggtttc gtcaggaacg cgctgaacgc
     2101 gtctcaggcg gtgcgggcaa cggatgttat ggtaaattct tgggggcttg attataaaat
     2161 accgcacttc ctcttatgtc ttgtgctttt tagacaaccc tgaaagcttt tagctttggt
     2221 gatgcaattt gctgggattt tggcggagaa ccacaggtaa agaaaaaggc cacattagcg
     2281 gcctttttcg gagaagatgc tcagtgcgga atttcggaga cttctttaac gtcggtttta
     2341 ttgatctgct gtttgacacc attcgcgtct gtatagccaa gcaggcctga gtctgattct
     2401 ttaggtttgc cgtcgctgac aatagtgcga ccgtcattgg tgtgtatggc atagcttgtg
     2461 cgcgtgcagc cggtgagggc tgaaagcgca actgccgctg cgaacactga aacaaaaatc
     2521 tttttcaaag ccagctcctt ttatccatat cagttttggt cactaaccat ctaactatag
     2581 gtcatttttt atgtgaaaag aggcttgatg gtgggctgta tatcgagtta ggcgcgaggt
     2641 gctatggtca aagtgtggtg tcaggcgggg aggaggctta tatgagatgt aatccggccg
     2701 atacatatac caagatgatt taagtattcc tctttgtgag gttggatgtc taatttataa
     2761 aggatcttct tgagatcctt tttttccgcg cgtaatctct tgccctgtaa acgaaaaaac
     2821 caccctggca ggtggttttt cgaaggttag gtaatcctgg cagatcccct aaccgtggta
     2881 acagtcttgt gcgagacatg tcaccaaatt tgtcctttca gtgtagcctc actaaggccg
     2941 ccacttcaag aactcttgag acatctctcg cacatcctgt ttgccaatgg ccgttgccaa
     3001 tggcgattag tcgtgtcttt ccgggttgga ctcaagttga tagttaccgg ataaggcgca
     3061 gcggtcggac tgaacggggg gttcgtgcat acagtccatc ctggagcgaa ctgccttccc
     3121 ggaactgagt gtcaggcgtg gaatgagaaa ccgcggccat aacagcggag tgacaccggt
     3181 aaaccgaaag gcaggaatgc gggggagcac gagggagcca ccagggggaa acgcctggta
     3241 tctttaagcc gcatcgggtt tcgccaccac tgatttgagc gtcagattct gtgatgcttg
     3301 tcaggggggc ggagcctatg gaaaaacggc tttggcgcgg ccttatgctt tcttcgttaa
     3361 gtatcttcct ggcatccccc aggaaatttc tgatccatcc gtaagcccgt cccgctcgcc
     3421 gcagccgaac gaccgagcgg agcgagtcag tgagcgagga agcggaatat attctgtatc
     3481 acattttctc ctgacgcgtt ttctttcact ttctgcgcct gtcttatgtg gcattaatgc
     3541 tatgtgttac tgccatgcta catcttaagc cagtatacac tccgctagtg ctccgtgact
     3601 ggtccggcgc tgcgcccgga acccgcctgt accggttcag cagccgttcc ggcctgactg
     3661 caattttttt ttttcatccc tgcccgctac cctgtaaacc tttcttctgc gttgccgtta
     3721 acagtaggta agccatgttt ggtttttctt ccggagccct gatgactctt agtgatgatg
     3781 agcgacacct gcttgtgtcc gttgtttcgg tctggctacg gcgtgccgga ggggatgccg
     3841 gagccatgat gctggatgca taccggcaga ttttgtctga aaccgaaccg gccgtaagga
     3901 ctgtcatgct cgaatttctt gaatccgttc gcattcatta catttcgtcc tgagcatttt
     3961 ctgtatttct gtccatactt aaacgaggcg gccgtcacgg acggtggtcg tcgttccatg
     4021 atgctgttat ggacgagtga gagtaatcta atctaacttg attgagttcc gggcgtgccg
     4081 cccggttttt ttttgctgtt tcccggtgtc cgttccggga caggatgtgc atttgtaata
     4141 ccgcgcacgc gccggcatca caaataccct ggtcagggct gcgccccgac accccgccat
     4201 cgcctcacgg ccgcgcactg aattgcgcac ccgttcaccg attttcacgc attttcctcg
     4261 cctatctgtg cgttcagcat ggcgtaaagc gcttcggcct gatcgtgcag gtgctcacag
     4321 cagccggcag cctcatccag atccatctgc tcgagccgct caaggaggag aagtgtctgt
     4381 gcgcggataa atttggccat attgagggcg gcggattcct gtctggtcac tggtgatttt
     4441 ccccgtctgg tgatgatttt tgcgtgaaaa gagcatggcg tcattctggt gcatagtcat
     4501 gcatgccgtt aaaatttaac aggcgcgttt ctgacgggtt ccggggtggt ttgttgtggt
     4561 ttttgttcat aggtttcgtc aggaacagcg ctgaacgcgt ctcaggcggt gcgggcaacg
     4621 gatgttatgg taaattcttg ggggcttgat tataaaaata cctgcacttc ctcttatgtc
     4681 ttgtgctttt tagacaaccc tgaaagcttt ttagctttgg tgatgcaatt tgctgggatt
     4741 ttggcggaga accacaggta aagaaaaagg ccacattagc ggcctttttt cggagaagga
     4801 ctgctcagtg cggaatttcg gagacttctt taacggtcgg ttttattgat tctgctgttt
     4861 gacaccattc gcgtctgtat agccaagcag gcctgagtct gattctttag gtttgccgtc
     4921 gctgacaaat agtgcgaccg tcattggtgt gtatggcata gcttgtgcgc gtgcagccgg
     4981 tgagggctga aagcgcaact gccgcctgcg aacactgaaa caaaacatct ttttcaaagc
     5041 cagctccttt tatccatatc agttttggtc actaaccatc taactatagg tcattttttt
     5101 atgtgaaaag agggcttgat gggtgggctg tatatcgagt taggcgcgag gtgctatggt
     5161 caaagtgtgg tgtcaggcgg ggaggaggct tatctgagat gtaatccggc cgatacatat
     5221 accaagatga tcttaagtat tcctctttgt gaggttggat gtctaattta taaaggatct
     5281 tcttgagatt cctttttttt cctgcgcgta atctcttgcc ctgtaaacga aaaaaccccc
     5341 ctggcaggtg ttttttcgaa ggttaggtaa tcctggcaga tccctaacct gtggctaaca
     5401 gtctgtgcga gacatgtcac caaatttgtc ctttcagtgt agcctcacta aggccgccac
     5461 ttcaagaact cttgagacat ctctcgcaca tcctgtttgc caatggccgt tgccaatggc
     5521 gattatgatc gtgtctttct tccgggttgg actcaagttg atagttaccg gataaggcgc
     5581 agcggtcgga ctgaacgggg gggttcggtg catacagtcc atcctggagc gaactgcctt
     5641 ccccggaacg gagtgtcagg cgtggaatga gaaaaccgcg gccaaaacag ctggagtgaa
     5701 accgtaaacc gaaaggcagg aatgcggggg agcacgaggg agtccaccag ggggaaactg
     5761 cctggtatct ttaagccgca tcgggtttcg ccaccactga tttgagcgtc agacttctgt
     5821 gatgcttgtc agggggggcg gagcctatga aaaacggctt tggcgcggcc ttatgctttc
     5881 ttcgttaagt atcttcctgg catcccccag gaaattactg atctcattcc cgtaagcccg
     5941 tcccgctcgc ctgcagccga acgaccgagc ggagcgagtc agtgagcgag gaagcggaat
     6001 atattctgta tcacattttc tcctgacgcg ttttctttca ctttctgcgc tgtcttatgt
     6061 ggcattaatg ctatgtgtta ctgccatgct acatcttaag ccagtataca ctccgctagt
     6121 gctccgtgac tggtccggcg ctgcgcccgg aacccgcctg taccggttca gcagccgttc
     6181 cggcctgact gcaatttttt cttttttcat ccctgtcccg ctacccctgt aaacctttct
     6241 tctgcgttgc cgttaacagt aggtaagcca tgtttggttt ttcttccgga gccctgatga
     6301 ctcttagtag atgatgagcg acacctgctg tgtcgtgttt cggtctgggc tacggcgtgc
     6361 cggaggggat ggaccggagc catgatgctg gatgcatacc ggcagatttt gtctgaaacc
     6421 gaaccggccg taaggactgt catgctcgaa tttcttgaat ccgttcgcac ttcattacat
     6481 tttcgtcctg agcattttc
//
