LOCUS       NZ_CP085750             2494 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 59732CZ plasmid p59732CZ_Col,
            complete sequence.
ACCESSION   NZ_CP085750
VERSION     NZ_CP085750.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377432
            Assembly: GCF_020695485.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 216x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695485.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:54:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,913
            CDSs (total)                      :: 4,797
            Genes (coding)                    :: 4,711
            CDSs (with protein)               :: 4,711
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 34 of 86
            Pseudo Genes (incomplete)         :: 51 of 86
            Pseudo Genes (internal stop)      :: 11 of 86
            Pseudo Genes (multiple problems)  :: 11 of 86
            Pseudo Genes (short protein)      :: 1 of 86
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085750.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="59732CZ"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p59732CZ_Col"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2020-09"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            46..264
                     /locus_tag="LJU46_RS23995"
                     /old_locus_tag="LJU46_23995"
     CDS             46..264
                     /locus_tag="LJU46_RS23995"
                     /old_locus_tag="LJU46_23995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     gene            complement(555..740)
                     /locus_tag="LJU46_RS24000"
                     /old_locus_tag="LJU46_24000"
     CDS             complement(555..740)
                     /locus_tag="LJU46_RS24000"
                     /old_locus_tag="LJU46_24000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1106..1333)
                     /locus_tag="LJU46_RS24005"
                     /old_locus_tag="LJU46_24005"
     CDS             complement(1106..1333)
                     /locus_tag="LJU46_RS24005"
                     /old_locus_tag="LJU46_24005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     Typing          421..508
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          complement(1749..2277)
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="88.598"
                     /coverage="88.0"
                     /evalue="0.0"
ORIGIN
        1 ccctgtaaac ctttcttctg cgttgccgtt aacagtaggt aagccatgtt tggtttttct
       61 tccggagccc tgatgactct tagtgatgat gagcgacacc tgcttgtgtc cgttgtttcg
      121 gtctggctac ggcgtgccgg aggggatgcc ggagccatga tgctggatgc ataccggcag
      181 attttgtctg aaaccgaacc ggccgtaagg actgtcatgc tcgaatttct tgaatccgtt
      241 cgcattcatt acatttcgtc ctgagcattt tctgtatttc tgtccatact taaacgaggc
      301 ggccgtcacg gacggtggtc gtcgttccat gatgctgtta tggacgagtg agagtaatct
      361 aatctaactt gattgagttc cgggcgtgcc gcccggtttt ttttgctgtt tcccggtgtc
      421 cgttccggga caggatgtgc atttgtaata ccgcgcacgc gccggcatca caaataccct
      481 ggtcagggct gcgccccgac accccgccat cgcctcacgg ccgcgcactg aattgcgcac
      541 ccgttcaccg attttcacgc attttcctcg cctatctgtg cgttcagcat ggcgtaaagc
      601 gcttcggcct gatcgtgcag gtgctcacag cagccggcag cctcatccag atccatctgc
      661 tcgagccgct caaggaggag aagtgtctgt gcgcggataa atttggccat attgagggcg
      721 gcggattcct gtctggtcac tggtgatttt ccccgtctgg tgatgatttt tgcgtgaaaa
      781 gagcatggcg tcattctggt gcatagtcat gcatgccgtt aaaatttaac aggcgcgttt
      841 ctgacgggtt ccggggtggt ttgttgtggt ttttgtcatg gtttcgtcag gaacgcgctg
      901 aacgcgtctc aggcggtgcg ggcaacggat gttatggtaa attcttgggg gcttgattat
      961 aaaataccgc acttcctctt atgtcttgtg ctttttagac aaccctgaaa gcttttagct
     1021 ttggtgatgc aatttgctgg gattttggcg gagaaccaca ggtaaagaaa aaggccacat
     1081 tagcggcctt tttcggagaa gatgctcagt gcggaatttc ggagacttct ttaacgtcgg
     1141 ttttattgat ctgctgtttg acaccattcg cgtctgtata gccaagcagg cctgagtctg
     1201 attctttagg tttgccgtcg ctgacaatag tgcgaccgtc attggtgtgt atggcatagc
     1261 ttgtgcgcgt gcagccggtg agggctgaaa gcgcaactgc cgctgcgaac actgaaacaa
     1321 aaatcttttt caaagccagc tccttttatc catatcagtt ttggtcacta accatctaac
     1381 tataggtcat tttttatgtg aaaagaggct tgatggtggg ctgtatatcg agttaggcgc
     1441 gaggtgctat ggtcaaagtg tggtgtcagg cggggaggag gcttatatga gatgtaatcc
     1501 ggccgataca tataccaaga tgatttaagt attcctcttt gtgaggttgg atgtctaatt
     1561 tataaaggat cttcttgaga tccttttttt ccgcgcgtaa tctcttgccc tgtaaacgaa
     1621 aaaaccaccc tggcaggtgg tttttcgaag gttaggtaat cctggcagat cccctaaccg
     1681 tggtaacagt cttgtgcgag acatgtcacc aaatttgtcc tttcagtgta gcctcactaa
     1741 ggccgccact tcaagaactc ttgagacatc tctcgcacat cctgtttgcc aatggccgtt
     1801 gccaatggcg attagtcgtg tctttccggg ttggactcaa gttgatagtt accggataag
     1861 gcgcagcggt cggactgaac ggggggttcg tgcatacagt ccatcctgga gcgaactgcc
     1921 ttcccggaac tgagtgtcag gcgtggaatg agaaaccgcg gccataacag cggagtgaca
     1981 ccggtaaacc gaaaggcagg aatgcggggg agcacgaggg agccaccagg gggaaacgcc
     2041 tggtatcttt aagccgcatc gggtttcgcc accactgatt tgagcgtcag attctgtgat
     2101 gcttgtcagg ggggcggagc ctatggaaaa acggctttgg cgcggcctta tgctttcttc
     2161 gttaagtatc ttcctggcat cccccaggaa atttctgatc catccgtaag cccgtcccgc
     2221 tcgccgcagc cgaacgaccg agcggagcga gtcagtgagc gaggaagcgg aatatattct
     2281 gtatcacatt ttctcctgac gcgttttctt tcactttctg cgcctgtctt atgtggcatt
     2341 aatgctatgt gttactgcca tgctacatct taagccagta tacactccgc tagtgctccg
     2401 tgactggtcc ggcgctgcgc ccggaacccg cctgtaccgg ttcagcagcc gttccggcct
     2461 gactgcaatt tttttttttc atccctgccc gcta
//
