LOCUS       NZ_CP085749             2811 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 59732CZ plasmid p59732CZ_1, complete
            sequence.
ACCESSION   NZ_CP085749
VERSION     NZ_CP085749.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377432
            Assembly: GCF_020695485.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 216x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695485.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 04:54:00
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,913
            CDSs (total)                      :: 4,797
            Genes (coding)                    :: 4,711
            CDSs (with protein)               :: 4,711
            Genes (RNA)                       :: 116
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 5
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 34 of 86
            Pseudo Genes (incomplete)         :: 51 of 86
            Pseudo Genes (internal stop)      :: 11 of 86
            Pseudo Genes (multiple problems)  :: 11 of 86
            Pseudo Genes (short protein)      :: 1 of 86
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085749.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="59732CZ"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p59732CZ_1"
                     /geo_loc_name="Czech Republic: South Bohemian Region"
                     /lat_lon="48.97 N 14.48 E"
                     /collection_date="2020-09"
                     /collected_by="Nemocnice Ceske Budejovice"
     gene            121..1404
                     /locus_tag="LJU46_RS23985"
                     /old_locus_tag="LJU46_23985"
     CDS             121..1404
                     /locus_tag="LJU46_RS23985"
                     /old_locus_tag="LJU46_23985"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="WP_227524674.1"
                     /translation="MKSQFMHVELYSREEPAKKTINKSKNINHKSDVRTTTVNGVLSEM
                     KREEGFTSHLETIAAPEVLYGSIDALERSIERYEAEFKTTDKNGKEKKLRKDACILLAG
                     VVSLDRADEEIWDEYKTKAIEYLKNKYGENLKCVVEHTDETNPHFHFYVVGNKDQDLNL
                     LHDGKLAVSKLAKEDKKKLHQTAYTEAMIKFQDDFYVQVSNKFGLSRTGEEPRERYKSR
                     PDYLRFKKRQETALNILANLEEFEEKELKKAKQAGFNVGIKNFDETNFFRKLDWRVKRI
                     KKEKDNEIKKLSDENDNLNIDKSNLQHELDMSKIELSDAYSQRNEASRKNSVYIDKVNA
                     LKKENKLLLPYKDQFDLKLKQEKEPLQAEVKELTSENTNLKNENTKLKKENLDFKEFIL
                     GVKNYYGSKFQEWYEEVFNPKTKTKPKF"
                     /name="plasmid recombination protein"
     gene            2089..2688
                     /locus_tag="LJU46_RS23990"
                     /old_locus_tag="LJU46_23990"
     CDS             2089..2688
                     /locus_tag="LJU46_RS23990"
                     /old_locus_tag="LJU46_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="WP_001029633.1"
                     /translation="MAKAKVLINDTDEAVIKNALDVEDMNEVNINVTRKKKTGFLGTYS
                     IVFHDSVKTLSKMNLKPNAYKIVMYLFGIIEMGNILVNFSQKKIADDLGLQPSNVSRAF
                     KELFDKGVLIKDSEDGHVYLNSNLCTMGVPKNFDQKRMKSLQKSNIETEDFKNQINLFT
                     PTNTAKIKTNDKKVIKKKEKENVLEFPMQNDWVDIE"
                     /name="Crp/Fnr family transcriptional regulator"
ORIGIN
        1 tggagcagca tatgaaatat gctagcgtaa tagatatagt cggttcctat agctattgac
       61 agaaaaacaa taaatgttaa ttacatatat aaggaaaatt aaaatatagg agaatttaaa
      121 atgaaatcac aatttatgca tgtagaactc tacagcagag aagaaccagc taaaaagaca
      181 ataaataaaa gtaaaaatat aaatcataaa tctgatgtta gaacaacaac cgtaaacggt
      241 gtgttgtcag aaatgaaaag ggaggagggt tttacatccc atttagaaac catagctgca
      301 ccggaggtgc tctatgggtc tatagatgcc ttagaacgct ctatagagcg ttacgaggcc
      361 gaatttaaga ctacagacaa gaacgggaag gagaagaagc tacgcaaaga tgcatgcatc
      421 ttgctagctg gtgtggtttc tctagatcga gctgatgaag aaatttggga tgaatataag
      481 accaaagcaa ttgaatattt aaaaaataaa tatggtgaaa atttaaaatg tgttgttgag
      541 catacagacg aaaccaaccc acattttcat ttttacgtcg ttggaaacaa ggatcaagat
      601 ttaaatttgc ttcatgatgg aaagctcgca gtatctaaat tagcaaaaga agataagaaa
      661 aaattacatc aaacagctta cacagaagct atgatcaaat ttcaagatga tttttatgta
      721 caggtatcaa ataaatttgg attgagcaga acgggcgagg agccgcgaga acgctataaa
      781 tcacgccctg attacttgag atttaaaaaa agacaagaaa cagcattaaa tatattagcc
      841 aatttagaag agtttgaaga aaaagaatta aaaaaagcaa aacaagctgg ctttaatgtt
      901 ggtattaaaa atttcgatga aactaatttt tttaggaaac tcgattggag agtaaaaaga
      961 ataaaaaaag aaaaagacaa tgagattaaa aaattatcgg atgaaaacga caatttaaat
     1021 atagataagt caaatttaca gcatgaatta gatatgagta aaattgagct ttcagatgca
     1081 tatagtcaaa ggaatgaagc atccagaaaa aacagcgttt atattgataa agtaaacgct
     1141 ctaaaaaaag aaaataaatt attacttcca tacaaagatc aatttgattt gaaattaaag
     1201 caggaaaaag agccattgca agcagaagtc aaagaattga cttctgaaaa taccaatttg
     1261 aaaaatgaga acacaaaatt aaaaaaagaa aatttagatt tcaaagaatt tattttgggt
     1321 gttaaaaatt actatggatc taaatttcag gaatggtacg aggaagtatt caacccaaaa
     1381 acaaaaacaa aacccaaatt ttaattgaat attttcatat ttgccgccta tgtggtgtat
     1441 gagacaccac cagaagcggc ctgacagcgt ttagctgtca tagctgggat ttatcccaaa
     1501 aaaagtggtt ttgcacgcag ttagttacct ttaaatatca gtcacttaaa tacaaaaaat
     1561 gcaggttggg aaaatgtggt cagggagctg gccgcattgg ggtgggggta ccttcataac
     1621 gccttggtgc gttgtgaaga gggggaacgg ggcggggttc cccctttggt atgtaactac
     1681 atataaaaca aagaaatact gttaaacttt gaaccgtgag aactgcagaa ccacttgtgg
     1741 ttctgtggtt cgaggggttg gcctgcccgg cgagggcggt tttgcagttg attttacggt
     1801 ttcttctttc ttattttctt acttcttaag caccctttaa agccttatat atcaacactt
     1861 taccaaaaaa aaaattatga aaatcataat tttcgattat gaaaatcata atgcaaaatt
     1921 atgaaaatca taatcagact tatgaaaatc ataattttgc atacaggtgc attgatatga
     1981 tttgatgttg aaacgataac tttcttatcg ttttgatatt gacattgcat ttagatcggt
     2041 tataaatgca acataggtta ttatttttat tagaaaaagg aatataaaat ggcaaaagca
     2101 aaagttttaa taaacgatac agatgaagca gtaataaaaa acgcattaga tgttgaagat
     2161 atgaacgaag taaatattaa cgtcacgaga aaaaagaaaa ccggattttt agggacttat
     2221 tcaatagtat ttcatgactc tgtgaaaaca ctatctaaaa tgaacttaaa gccaaatgct
     2281 tataagattg taatgtactt attcggcatt atagagatgg gtaatatttt agttaacttt
     2341 tcacaaaaga agatagctga tgatttaggc ttgcagccgt ctaatgtatc aagagctttc
     2401 aaagagcttt ttgataaagg ggttttaatc aaagatagcg aagacggaca cgtttactta
     2461 aattcaaatc tatgcactat gggtgttccg aaaaattttg atcaaaagag aatgaaaagc
     2521 ttacagaaat caaatattga aactgaagat tttaaaaatc agattaattt atttactcca
     2581 acaaacaccg caaaaataaa aactaatgat aaaaaagtta tcaagaaaaa agaaaaagaa
     2641 aacgttttag agtttccaat gcaaaatgat tgggttgata ttgaatagta aaactaacac
     2701 taccgcactt cgtttgctga atccctaaac ctcgcaaata taaaatttgc aagctttagg
     2761 gatttgcttt tttgttttga agttgacttt aaccccgtag ggccgcatct a
//
