LOCUS       NZ_CP085745             2495 bp    DNA     circular CON 29-APR-2024
DEFINITION  Enterobacter hormaechei strain 60214CZ plasmid p60214CZ_Col,
            complete sequence.
ACCESSION   NZ_CP085745
VERSION     NZ_CP085745.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22377434
            Assembly: GCF_020695365.1
KEYWORDS    RefSeq.
SOURCE      Enterobacter hormaechei
  ORGANISM  Enterobacter hormaechei
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Enterobacter; Enterobacter cloacae complex.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Bitar,I., Papagiannitsis,C.C., Kraftova,L., Marchetti,V.M.,
            Petinaki,E., Finianos,M., Chudejova,K., Zemlickova,H. and Hrabak,J.
  TITLE     Implication of different replicons in the spread of the
            VIM-1-encoding integron, In110, in Enterobacterales from Czech
            hospitals
  JOURNAL   Front Microbiol 13, 993240 (2023)
   PUBMED   36687644
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Bitar,i.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-OCT-2021) Microbiology, Charles University in Prague,
            Alej Svobody 1655/76, Plzen 1 32300, Czech Republic
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Microbial assembly v. v.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 281x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_020695365.1-RS_2024_04_29
            Annotation Date                   :: 04/29/2024 05:31:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,010
            CDSs (total)                      :: 4,892
            Genes (coding)                    :: 4,761
            CDSs (with protein)               :: 4,761
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 131
            CDSs (without protein)            :: 131
            Pseudo Genes (ambiguous residues) :: 0 of 131
            Pseudo Genes (frameshifted)       :: 50 of 131
            Pseudo Genes (incomplete)         :: 92 of 131
            Pseudo Genes (internal stop)      :: 20 of 131
            Pseudo Genes (multiple problems)  :: 30 of 131
            Pseudo Genes (short protein)      :: 1 of 131
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085745.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Enterobacter hormaechei"
                     /mol_type="genomic DNA"
                     /strain="60214CZ"
                     /isolation_source="tracheal aspirate"
                     /host="Homo sapiens"
                     /db_xref="taxon:158836"
                     /plasmid="p60214CZ_Col"
                     /geo_loc_name="Czech Republic: Prague"
                     /lat_lon="50.07 N 14.43 E"
                     /collection_date="2020-10"
                     /collected_by="Nemocnice Na Homolce"
     gene            593..820
                     /locus_tag="LJU47_RS22445"
                     /old_locus_tag="LJU47_22445"
     CDS             593..820
                     /locus_tag="LJU47_RS22445"
                     /old_locus_tag="LJU47_22445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615788.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="WP_021243043.1"
                     /translation="MKKIFVSVFAAAVALSALTGCTRTSYAIHTNDGRTIVSDGKPKES
                     DSGLLGYTDANGVKQQINKTDVKEVSEIPH"
                     /name="YgdI/YgdR family lipoprotein"
     gene            1186..1371
                     /locus_tag="LJU47_RS22450"
                     /old_locus_tag="LJU47_22450"
     CDS             1186..1371
                     /locus_tag="LJU47_RS22450"
                     /old_locus_tag="LJU47_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_013772937.1"
                     /translation="MTRQESAALNMAKFIRAQTLLLLERLEQMDLDEAAGCCEHLHDQA
                     EALYAMLNAQIGEENA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1662..1880)
                     /locus_tag="LJU47_RS22455"
                     /old_locus_tag="LJU47_22455"
     CDS             complement(1662..1880)
                     /locus_tag="LJU47_RS22455"
                     /old_locus_tag="LJU47_22455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_198129632.1"
                     /translation="MFGFSSGALMTLSDDERHLLVSVVSVWLRRAGGDAGAMMLDAYRQ
                     ILSETEPAVRTVMLEFLESVRIHYISS"
                     /name="hypothetical protein"
     Typing          complement(1419..1506)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="6.86e-39"
     Typing          2..358
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="80.952"
                     /coverage="99.0"
                     /evalue="2.48e-71"
     Typing          2397..2495
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.089"
                     /coverage="98.0"
                     /evalue="2.0900000000000002e-29"
ORIGIN
        1 gggaaggcag ttcgctccag gatggactgt atgcacgaac cccccgttca gtccgaccgc
       61 tgcgccttat ccggtaacta tcaacttgag tccaacccgg aaagacacga ctaatcgcca
      121 ttggcaacgg ccattggcaa acaggatgtg cgagagatgt ctcaagagtt cttgaagtgg
      181 cggccttagt gaggctacac tgaaaggaca aatttggtga catgtctcgc acaagactgt
      241 taccacggtt aggggatctg ccaggattac ctaaccttcg aaaaaccacc tgccagggtg
      301 gttttttcgt ttacagggca agagattacg cgcggaaaaa aaggatctca agaagatcct
      361 ttataaatta gacatccaac ctcacaaaga ggaatactta aatcatcttg gtatatgtat
      421 cggccggatt acatctcata taagcctcct ccccgcctga caccacactt tgaccatagc
      481 acctcgcgcc taactcgata tacagcccac catcaagcct cttttcacat aaaaaatgac
      541 ctatagttag atggttagtg accaaaactg atatggataa aaggagctgg ctttgaaaaa
      601 gatttttgtt tcagtgttcg cagcggcagt tgcgctttca gccctcaccg gctgcacgcg
      661 cacaagctat gccatacaca ccaatgacgg tcgcactatt gtcagcgacg gcaaacctaa
      721 agaatcagac tcaggcctgc ttggctatac agacgcgaat ggtgtcaaac agcagatcaa
      781 taaaaccgac gttaaagaag tctccgaaat tccgcactga gcatcttctc cgaaaaaggc
      841 cgctaatgtg gcctttttct ttacctgtgg ttctccgcca aaatcccagc aaattgcatc
      901 accaaagcta aaagctttca gggttgtcta aaaagcacaa gacataagag gaagtgcggt
      961 attttataat caagccccca agaatttacc ataacatccg ttgcccgcac cgcctgagac
     1021 gcgttcagcg cgttcctgac gaaaccatga caaaaaccac aacaaaccac cccggaaccc
     1081 gtcagaaacg cgcctgttaa attttaacgg catgcatgac tatgcaccag aatgacgcca
     1141 tgctcttttc acgcaaaaat catcaccaga cggggaaaat caccagtgac cagacaggaa
     1201 tccgccgccc tcaatatggc caaatttatc cgcgcacaga cacttctcct ccttgagcgg
     1261 ctcgagcaga tggatctgga tgaggctgcc ggctgctgtg agcacctgca cgatcaggcc
     1321 gaagcgcttt acgccatgct gaacgcacag ataggcgagg aaaatgcgtg aaaatcggtg
     1381 aacgggtgcg caattcagtg cgcggccgtg aggcgatggc ggggtgtcgg ggcgcagccc
     1441 tgaccagggt atttgtgatg ccggcgcgtg cgcggtatta caaatgcaca tcctgtcccg
     1501 gaacggacac cgggaaacag caaaaaaaac cgggcggcac gcccggaact caatcaagtt
     1561 agattagatt actctcactc gtccataaca gcatcatgga acgacgacca ccgtccgtga
     1621 cggccgcctc gtttaagtat ggacagaaat acagaaaatg ctcaggacga aatgtaatga
     1681 atgcgaacgg attcaagaaa ttcgagcatg acagtcctta cggccggttc ggtttcagac
     1741 aaaatctgcc ggtatgcatc cagcatcatg gctccggcat cccctccggc acgccgtagc
     1801 cagaccgaaa caacggacac aagcaggtgt cgctcatcat cactaagagt catcagggct
     1861 ccggaagaaa aaccaaacat ggcttaccta ctgttaacgg caacgcagaa gaaaggttta
     1921 cagggtagcg ggcagggatg aaaaaaaaaa aattgcagtc aggccggaac ggctgctgaa
     1981 ccggtacagg cgggttccgg gcgcagcgcc ggaccagtca cggagcacta gcggagtgta
     2041 tactggctta agatgtagca tggcagtaac acatagcatt aatgccacat aagacaggcg
     2101 cagaaagtga aagaaaacgc gtcaggagaa aatgtgatac agaatatatt ccgcttcctc
     2161 gctcactgac tcgctccgct cggtcgttcg gctgcggcga gcgggacggg cttacggatg
     2221 gatcagaaat ttcctggggg atgccaggaa gatacttaac gaagaaagca taaggccgcg
     2281 ccaaagccgt ttttccatag gctccgcccc cctgacaagc atcacagaat ctgacgctca
     2341 aatcagtggt ggcgaaaccc gatgcggctt aaagatacca ggcgtttccc cctggtggct
     2401 ccctcgtgct cccccgcatt cctgcctttc ggtttaccgg tgtcactccg ctgttatggc
     2461 cgcggtttct cattccacgc ctgacactca gttcc
//
