LOCUS       NZ_CP085626            12913 bp    DNA     linear   CON 04-DEC-2023
DEFINITION  Escherichia coli strain EC71 plasmid unnamed3.
ACCESSION   NZ_CP085626
VERSION     NZ_CP085626.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22488136
            Assembly: GCF_020683085.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12913)
  AUTHORS   Shihan,Z., Xiaoyan,L., Jiajun,L., Aiwu,W. and Chao,Z.
  TITLE     Molecular Epidemiology and Characteristics of CTX-M-55
            Extended-Spectrum Beta-Lactamase -producing Escherichia coli from
            Guangzhou, China
  JOURNAL   Front Microbiol (2021) In press
REFERENCE   2  (bases 1 to 12913)
  AUTHORS   Shihan,Z., Xiaoyan,L., Jiajun,L., Aiwu,W. and Chao,Z.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-OCT-2021) KingMed School of Laboratory Medicine,
            Guangzhou Medical University, 195 Dongfeng West Road, Guangzhou,
            Guangdong 510000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method :: SPAdes v. 3.13.1; unicycler v. 0.4.8
            Assembly Name :: Escherichia coli EC71 carrying CTX-M-55 in
            Guangzhou
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 110.0x
            Sequencing Technology :: Illumina NovaSeq; Oxford Nanopore
            PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 12/04/2023 04:52:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,143
            CDSs (total)                      :: 5,023
            Genes (coding)                    :: 4,782
            CDSs (with protein)               :: 4,782
            Genes (RNA)                       :: 120
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 241
            CDSs (without protein)            :: 241
            Pseudo Genes (ambiguous residues) :: 0 of 241
            Pseudo Genes (frameshifted)       :: 83 of 241
            Pseudo Genes (incomplete)         :: 166 of 241
            Pseudo Genes (internal stop)      :: 47 of 241
            Pseudo Genes (multiple problems)  :: 50 of 241
            Pseudo Genes (short protein)      :: 3 of 241
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085626.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..12913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC71"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="China: Guangzhou, Guangdong"
                     /lat_lon="22.26 N 112.57 E"
                     /collection_date="2020-06-30"
                     /collected_by="a patient in a tertiary hospital"
     gene            <1..396
                     /locus_tag="LJN53_RS25560"
                     /old_locus_tag="LJN53_25560"
                     /pseudo=""
     CDS             <1..396
                     /locus_tag="LJN53_RS25560"
                     /old_locus_tag="LJN53_25560"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="incomplete; too short partial abutting assembly gap;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            393..1034
                     /locus_tag="LJN53_RS25565"
                     /old_locus_tag="LJN53_25565"
     CDS             393..1034
                     /locus_tag="LJN53_RS25565"
                     /old_locus_tag="LJN53_25565"
                     /inference="COORDINATES: protein motif:HMM:NF016255.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="WP_217823325.1"
                     /translation="MTEPTRPILTIKRRNRTVYVPPEKAAKSEPQPAERTTSKAKVQPK
                     AKAPQKPAQKPTAAKPKKTPEQIAEGIRRQEENDRRQQDIAIANKSKKALANRVTKAFL
                     KEHWPDLLTRGVDRRPMAKGIIEQILTRGAELDMPPEADRKAVRRILGIVTGTSSYQQS
                     LIDQCMRYDLDGNPTEPATPEEAENAKARLTEIKAFRRKARQAKGEKKGA"
                     /name="ProQ/FINO family protein"
     gene            complement(1232..1594)
                     /locus_tag="LJN53_RS25570"
                     /old_locus_tag="LJN53_25570"
     CDS             complement(1232..1594)
                     /locus_tag="LJN53_RS25570"
                     /old_locus_tag="LJN53_25570"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186829.1"
                     /translation="MRKLPRHLQGEVLMPLSSRITRSQRLELIWLLPVLRLHPGDRSVT
                     ERRALGRFRGSLAAGGQTDAFVRSDASLYALGLRFQRKRTRELVASDPDVAAAGEALEQ
                     RANGVNLSETFGGGDE"
                     /name="hypothetical protein"
     gene            complement(1587..2159)
                     /locus_tag="LJN53_RS25575"
                     /old_locus_tag="LJN53_25575"
     CDS             complement(1587..2159)
                     /locus_tag="LJN53_RS25575"
                     /old_locus_tag="LJN53_25575"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301021.1"
                     /translation="MSAFKLLCDRLRNEATLAKSDGAGAFKKQSLDPDLKGGKKGKKPA
                     GELEEDEAIASLHRKRMNDVKGCKTLTKSTGADDFGLPPLSSVVETVVETGGAAQTFAP
                     IGAPVASPTEAVHRARAQSIQDAAFLNEMKAAGVAPADLVLLDGVLTSSRGMPDAARAP
                     AFAEVRRQVQAGGMTLSPAARGIDWNA"
                     /name="hypothetical protein"
     gene            complement(2159..2818)
                     /locus_tag="LJN53_RS25580"
                     /old_locus_tag="LJN53_25580"
     CDS             complement(2159..2818)
                     /locus_tag="LJN53_RS25580"
                     /old_locus_tag="LJN53_25580"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217823326.1"
                     /translation="MTEIKKESADDAWLRSTLAELAGDAFPMYDLPSLQVDTNSSIQLS
                     ALADRQAAREMRQAASDVEARRLDAARVVEGLKTEAERLRGLIADGKAALRAGEPVSPD
                     AGVASFLLPDIEAELVVAEAAEADVARERDTLLQDADRRDAAAALALFNWAHSVRVQRI
                     ELLLKLAMDEATTLAEADGGRGLYRTVIAPDRRLNQIFATQGAIEILRRNRGMEGV"
                     /name="hypothetical protein"
     gene            3078..3665
                     /locus_tag="LJN53_RS25585"
                     /old_locus_tag="LJN53_25585"
     CDS             3078..3665
                     /locus_tag="LJN53_RS25585"
                     /old_locus_tag="LJN53_25585"
                     /inference="COORDINATES: protein motif:HMM:NF012464.3"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_064186842.1"
                     /translation="MNKTYGYVRVSTDEQTSNSQRDAIIARFPTASILADDGISGTVPP
                     RERPALGILLNQLLQEGDTLVIYAIDRIGRNARDTLELVEELEKRKITLVSIREGVDLS
                     SAVGQAVLGVMASFAQMERKLILERQAAGIEAAKSRGQKFGRPTKIGAKEAGEIVMRRA
                     MGVPIATIAKQLNLSRSAIYRHLEKVDAVAPE"
                     /name="recombinase family protein"
     gene            complement(3746..4000)
                     /locus_tag="LJN53_RS25590"
                     /old_locus_tag="LJN53_25590"
     CDS             complement(3746..4000)
                     /locus_tag="LJN53_RS25590"
                     /old_locus_tag="LJN53_25590"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162734320.1"
                     /translation="MLLTPVRLSLWSCLPCVKADDADGAVSPCLVAEGSLVLASAVGGA
                     MTRLAKLADLAVFDAQARVAGRGGLLYALHVIKPLTGGW"
                     /name="hypothetical protein"
     gene            4218..4685
                     /locus_tag="LJN53_RS25595"
                     /old_locus_tag="LJN53_25595"
     CDS             4218..4685
                     /locus_tag="LJN53_RS25595"
                     /old_locus_tag="LJN53_25595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008324036.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_064186840.1"
                     /translation="MKKYTTEQKAQALRLLEQDGATSATVARTMGIPPRTVRRWASEKA
                     AAPSNVLSIEEMRKRAAAAVEATPQAAIRRLKNHFVQQQFDLLQRHAKDLQALRSASLQ
                     AMLEKDATMVKAISGLMTALLKAQERERLIYEIKPGTEADIMREGMNRKQQ"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5360..6931)
                     /locus_tag="LJN53_RS25600"
                     /old_locus_tag="LJN53_25600"
     CDS             complement(5360..6931)
                     /locus_tag="LJN53_RS25600"
                     /old_locus_tag="LJN53_25600"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217823327.1"
                     /translation="MSNHEENSGAAADNVTPITAAPSPLSLKLGDALFSVLSVSADWSG
                     DYRAQFELYGLNVKAIKSAVGTAVWHAGKGRFLSVLNGSLTEFDKGDGMKLLEDSCGKF
                     WHRTDAFIARLTDLKIKTDDKVTKACIDMARAVRQAVAEFIMLRRQVAVVRLDVDMFAT
                     APRVELVGETVTFVRPHAPYPVANADSDVVADWLVHFPQCHEFLDALVAARFASSRKNA
                     YLFFRAQSDWGKGLLFGAGGVLSRLGATVELSEGELLNILSGANSGVTASHFMGALALI
                     VNECTRVTKKHFRLEESLALTPKYLTTQCVNLYMKIFTSADPIPGLSDSDGIDPQVANR
                     FSMLDLQGDIKTRPLFLSDKGRYVDSLTSFFAAELNARVASYQAKGFETARRDADHVLA
                     AFTTAHGLANAAGLITDAYDEIRAEWVAFVHGRVADGHPDFLTFESKAGLVLRSPVGCW
                     GKFLDWYVDKSEPLRRARLMHDRDLIIGCAKKYRDVGGAAEGKGVLIPFVSGSMPDNVH
                     RFRYKN"
                     /name="hypothetical protein"
     gene            7456..7944
                     /locus_tag="LJN53_RS25605"
                     /old_locus_tag="LJN53_25605"
     CDS             7456..7944
                     /locus_tag="LJN53_RS25605"
                     /old_locus_tag="LJN53_25605"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_061301014.1"
                     /translation="MREKTRTLTVRLPVSLIERIEQRAEKNKVTRTDAVIEALVAGLGT
                     KPEGMDTKLLTAIEALRSEVRALAGGGKDARQSAPQTARQSAPQNARQIVPRGADPRFQ
                     AVSDEADQLIVSMTGENATLPAIAQALNAAGHTTATGQEWNTSRVRDYRHKLRERGLI"
                     /name="hypothetical protein"
     gene            complement(7962..8420)
                     /locus_tag="LJN53_RS25610"
                     /old_locus_tag="LJN53_25610"
     CDS             complement(7962..8420)
                     /locus_tag="LJN53_RS25610"
                     /old_locus_tag="LJN53_25610"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217823328.1"
                     /translation="MKSKTELRAAATARALEVIASEMGGWSLDGFAHGSLPNFSPLPRQ
                     QTQEGSVVLERPPFDYTWAGTAAFTDRANRALQVKLPASRERNYIWLCAVEREAVATAL
                     MVESFNVTGCAAFAGLPPVDGMVLLTMDEADVELIRAAMLQWLDAAAA"
                     /name="hypothetical protein"
     gene            complement(8651..9823)
                     /locus_tag="LJN53_RS25615"
                     /old_locus_tag="LJN53_25615"
     CDS             complement(8651..9823)
                     /locus_tag="LJN53_RS25615"
                     /old_locus_tag="LJN53_25615"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_145062813.1"
                     /translation="MAFVDFDTTHLKEDPGLFWRAVSAAGAPADRDEWVRLCLALGCWA
                     SLCGEDGLAAIEAWSTRHGRGENRHRQQYRSFLKGDLDTFAAQAFLNFSGVEWRNGRAS
                     VQSDVDRAERLRLSREATAKAKAERDKKGDAAARAAVKLLAKGGDPASTPYWRKKTALA
                     AAGGVQLPALSTDLGVGGMFRSFNPRAYWSDCLLIPVYKFDAARQLYVDGLEAICGRCS
                     AEGFQLTGSKFSPAGTRKQGGFYPMPGFMDVLDGSPHAPYSPLFVCEGFATAFALHHLS
                     RGKVAVCAAFSIAGFGSCVASLRRFWPDVDVMLVPDHGEAYRLAHKAAYGDEREPVPTI
                     PGAAVVSFDGLDRFTPRDNLDWFDVLVEEGEDRARELLRYAVRHARLERR"
                     /name="hypothetical protein"
     gene            complement(9810..10019)
                     /locus_tag="LJN53_RS25620"
                     /old_locus_tag="LJN53_25620"
     CDS             complement(9810..10019)
                     /locus_tag="LJN53_RS25620"
                     /old_locus_tag="LJN53_25620"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064186834.1"
                     /translation="MKKRIYTADLAALLNVSLRTLSRLSSRGIVPTPAGRSLRGGHYWL
                     AGNPEIKRFVADQRRPGRAADGVR"
                     /name="hypothetical protein"
     gene            10375..11295
                     /locus_tag="LJN53_RS25625"
                     /old_locus_tag="LJN53_25625"
     CDS             10375..11295
                     /locus_tag="LJN53_RS25625"
                     /old_locus_tag="LJN53_25625"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_217823329.1"
                     /translation="MNDDNDTNESTQPNPAALERVSATEKFPEQLPGVLFVTCPGGCSL
                     RVEACRQIYEQSHLDRTIENPHLECHRCPTGLALWVGTEQPPDAPLTTPADTRKLCPRC
                     SRKDNRLIAPNKPGSSLCVSCWNREREATIGANARGRVNRTPPFVSPFLIGIGDGWQVW
                     YGIHQGEALVRAMRANPGARFHDRRPGPVGQTRRDGFVYYCHEHREPLRWNGGDGHAGE
                     VELACPRCQPWAAPLSVAPTFQTVSFQTPHDAAHQYRNATLPDNAVWTASVCSDCKAAP
                     LVISKAPVKGKPRREIVVSWALLQS"
                     /name="hypothetical protein"
     gene            complement(11329..12033)
                     /locus_tag="LJN53_RS25630"
                     /old_locus_tag="LJN53_25630"
     CDS             complement(11329..12033)
                     /locus_tag="LJN53_RS25630"
                     /old_locus_tag="LJN53_25630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067855.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPL
                     GEMRLVSRVFEM"
                     /name="IS6-like element IS26 family transposase"
     gene            12223..12684
                     /locus_tag="LJN53_RS25635"
                     /old_locus_tag="LJN53_25635"
     CDS             12223..12684
                     /locus_tag="LJN53_RS25635"
                     /old_locus_tag="LJN53_25635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014198269.1"
                     /GO_component="GO:0005886 - plasma membrane [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="WP_196451626.1"
                     /translation="MQNILMNLAFYLLVVAAGASFSLQQAANNHLRAELLSPWWAGFIS
                     YVGGSLAMLVMALVCRGPGLSWDMLSRTSPFSWTGGILGAIYIATAIFMIPKLGATTVL
                     ALMVVGQMLLSLMLDHYGLLGIPVYPVTGIRLLGVVLLVLGVVLIRWQA"
                     /name="DMT family transporter"
     gene            <12686..12859
                     /locus_tag="LJN53_RS25640"
                     /old_locus_tag="LJN53_25640"
                     /pseudo=""
     CDS             <12686..12859
                     /locus_tag="LJN53_RS25640"
                     /old_locus_tag="LJN53_25640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /name="IS6 family transposase"
ORIGIN
        1 gtctcctgcc cgtcctgcga ctccagcgca tcagccaggc ccggaggcag gacatggaca
       61 gacgagcaca tcgcggtgcc cgagttcacg cagaacgaga cgcgcagcct ggcgaagatc
      121 cccgttattt tgccgcagag ggagcagccg cagctgctca aaagaccagg gacagggcgc
      181 ggtccaggac gcccccgcaa gcgcatcccc ctgaccctcg accgggtacc agagagtcgg
      241 ttcaaccgcc tcaccctttc cgtccttgcc gccgtcgggc gctgggacta catcaacgac
      301 atcatccggc gcatcgatcg ggaccgcggc gacgccgcca ccctgatcgc ccttgccgac
      361 cacatccaga ccaacagacc tgagagagat ttatgaccga acccacccgc ccgatcctga
      421 ccatcaagcg acgcaaccgc acggtctacg tcccgccgga aaaggccgcg aaatctgagc
      481 cacagccagc cgaacggact accagcaagg cgaaggtaca gccaaaagcg aaagccccgc
      541 agaagcccgc acagaagccc acagcggcaa agccgaaaaa gacgcccgag cagatcgccg
      601 aggggatccg cagacaggag gagaacgacc gccgccagca ggacatcgcg atcgccaaca
      661 agtccaaaaa ggcgctggcc aaccgggtga ccaaagcatt cctgaaagag cactggcctg
      721 acttactgac ccggggagtt gaccgtcgcc ccatggcaaa gggcatcatc gagcagatcc
      781 tgacccgagg cgcagagctg gacatgccgc cagaggcaga ccgcaaagcc gtccgtcgca
      841 tcctgggcat tgtcacgggt accagctcgt accagcagag cctcatcgac cagtgcatgc
      901 gctacgacct ggacggcaac cccaccgaac cagcaacccc ggaggaggcc gaaaacgcca
      961 aagcccgcct gacagagatc aaggcattcc ggcgcaaggc acgccaggcg aagggcgaaa
     1021 aaaagggcgc ataacgcgcc ccgtctcacc gtttcaccgc cctgtcgacc ggggctgttc
     1081 ccgtctcacc cagcgccacg taggcgcagc acgacgctct gcagcacggc gagcagctgc
     1141 gacacgttga gcctgtcccc ttgcccgtcg ggcagcaatg accccgtcgg ggatcaccac
     1201 ctcaggaatg accaggcgcg agacagcaaa ttcactcatc gccacccccg aacgtctcgg
     1261 acaggttcac gccgtttgca cgctgctcca gcgcctcgcc agccgcagca acatcgggat
     1321 ccgaagcaac cagctcccgg gtgcgcttgc gctggaaccg cagtcccagc gcgtacagag
     1381 acgcatccga acggacgaaa gcatccgtct ggccacccgc agcgagcgat ccccgaaacc
     1441 tgccaagcgc ccgccgctca gtgaccgatc gatcgcccgg gtggagccgc agaacgggca
     1501 acagccagat cagctccagc cgttgcgagc gcgtgatcct gctggacagc ggcatcaaca
     1561 cctcgccctg caggtgacgc ggcagcttac gcattccagt caataccacg cgcagccggt
     1621 gacagcgtca tgccacccgc ctgaacctga cgacgcacct cggcaaacgc cggagcacgc
     1681 gccgcgtctg gcatcccacg agaggacgtc aacacgccgt caagtaacac caggtcagcc
     1741 ggtgccacac ccgccgcttt catctcgttc aggaacgccg cgtcctggat ggactgcgca
     1801 cgcgcacggt gaaccgcttc ggtcggagac gccaccggag ccccgatcgg cgcgaacgtc
     1861 tgcgctgcgc cgcctgtctc caccactgtc tcgaccaccg acgacagcgg agggaggcca
     1921 aagtcatcag cgccggtgga tttggtcagc gtcttgcaac ctttaacgtc gttcatgcgc
     1981 ttacggtgga gagacgcgat cgcctcgtcc tcctccagct ccccagcggg ctttttgccc
     2041 ttcttgccgc ccttcaggtc gggatccaga gattgctttt tgaaagcgcc ggccccgtca
     2101 gattttgcca gcgtcgcctc attgcgaagc cggtcacaca ggagtttgaa agcagacatt
     2161 acacaccctc catgccacgg ttacggcgca ggatttcaat agcgccctgc gtggcgaaaa
     2221 tttggttaag acggcggtca ggcgcgatca cggtacggta caggccgcgc ccgccgtcgg
     2281 cttcagcgag cgtcgtcgcc tcgtccatcg ccaacttgag aagcagctcg atacgctgca
     2341 cgcgaacgga gtgcgcccag ttgaacagcg ccagcgcagc cgccgcgtca cggcgatcgg
     2401 catcctgcag cagggtgtca cgctcccggg ccacgtcggc ctcagcagcc tcagcaacaa
     2461 ccagctcggc ctcgatgtcc ggcagcagga acgacgcaac acccgcgtcg gggctcaccg
     2521 gctcgccagc acgcagcgca gccttgccgt cggcgatcaa gccccgcagc cgttcggcct
     2581 cggtcttgag cccttccacc actcgagcgg cgtccagccg tcgcgcttcc acgtcggaag
     2641 cggcctgtcg catttcacgc gcagcctgac gatcggcgag cgccgagagc tgaatggagg
     2701 agttcgtgtc tacctgcagg gacgggaggt catacatagg gaacgcatca cccgccaatt
     2761 cggcaagggt agagcgtagc caagcgtcgt cggcgctctc tttcttaatt tcggtcatgg
     2821 tttggttctc agcaagttat cagcaaagcg gcaagaaacg ccgctcagac agcatgccgt
     2881 cacgacgcag cgcagacatc aacgcagagg gcgcagggct ctcgcccagc gcggggagcc
     2941 gcctggaggc cgtcgctaat cccctacatt tcaggacacc caacagaacg acgaaaaaga
     3001 cactttagcg atagactttc gatacagttg tggcatgttg tgtcgaaaga gacgtatcgg
     3061 tcagaggaga gaaaaaagtg aacaaaacat acggttacgt tcgcgtatca accgacgaac
     3121 aaaccagcaa cagccagcga gacgccatca tcgcccggtt cccaaccgcc tccatccttg
     3181 ccgacgacgg gatcagcggg acggtaccac cacgcgagcg ccctgccctc ggcatcctct
     3241 tgaaccagtt gctgcaggag ggcgacaccc tggtcattta cgcgatcgat cgcatcggca
     3301 ggaacgccag ggacaccctc gagctggtcg aggagctgga gaagcggaag atcaccctgg
     3361 tcagcatccg cgagggcgtc gacctgagca gcgccgtcgg acaggcggta ctcggcgtaa
     3421 tggccagctt cgcccagatg gaacgcaagc tcatcctcga acgccaggca gcaggtatcg
     3481 aggcagcaaa gagccgaggc cagaagttcg gacgcccgac caagatcgga gcgaaggaag
     3541 ccggggagat tgtcatgcgc cgcgccatgg gagtgccgat cgccaccata gccaaacagc
     3601 taaacctaag ccggagcgcg atttaccgcc atcttgagaa ggtcgacgcg gtcgcgccgg
     3661 agtaagagag agatcgcggc ggtggtactc gctaaagtca ccgccgcaac ccaggcccgg
     3721 ggaagtgtga gagccaaccc gggcatcacc aaccacccgt tagaggttta atgacatgca
     3781 gagcatacaa cagaccaccc ctgcccgcta cccgggcctg agcatcgaac acggcgagat
     3841 ccgccagttt ggcaagccgc gtcattgcgc cgccaaccgc cgaggccagt accagactac
     3901 cctccgcgac caggcagggc gataccgcac cgtcagcatc atcagccttt acgcatggga
     3961 ggcatgacca taacgaaagc cttacaggtg taagcagcaa gcaataacaa aagccttacg
     4021 ccattacgaa ccacttacag gggcgcggaa gcgccccctt caccagaacg caccagagcc
     4081 attctaagcg cctcagccac aaaaacaccc aaccgcacga ccacgcacgc acaaacgccg
     4141 tgtacccccc aaaaaccccc gccattttca gcgcgtgacg tttcaatacg ggagagaaac
     4201 caaccggagc aggaaccatg aagaaataca ccaccgaaca gaaagcccag gcgctcaggc
     4261 tactggagca ggacggcgca accagtgcca ccgtcgcacg caccatgggg atcccacccc
     4321 gcaccgttcg ccgatgggcg agcgagaagg ccgcagcacc cagcaacgtc ctcagcatcg
     4381 aggagatgcg gaaacgcgcc gccgccgccg tcgaggccac accacaggcc gcgatccgac
     4441 gccttaaaaa ccactttgta cagcagcaat ttgacctgct acagcgccac gcaaaggact
     4501 tgcaagccct ccgcagcgca tcgttacagg ccatgctgga gaaggacgcc accatggtca
     4561 aagcgatcag cggcctcatg accgccctgc tcaaagcgca ggaacgcgaa cgcctcatct
     4621 acgagatcaa gccgggcacc gaggccgaca tcatgcgcga gggcatgaac cgcaaacaac
     4681 aatgaccgcc atgaccgaaa tgaccgccat gtgaccgttt ttgtgaccgc caaaacccgg
     4741 gagcccacgg aatacgcgga tccgcacgtt acgcaattgt taccgctcgc gtgcgcgaga
     4801 agccccgttt tgatgcccgg acgaaccaaa cgcgcagctg cagctacgca ttccacccat
     4861 aacgcagagg gcgcgagggc aagccctcac gcagaaccaa cagacgacca cccagcgcga
     4921 gcgacagctc gtgccctcag cgccacccca accacccgat ccacccccta accaccacca
     4981 cccaccaccc cggcacgacg aacccatcca ccgatcgacc aagacaagca aagtgcatcc
     5041 cgcagggtgc agctgcggcg cgtcaactgc gcaggaattg agctggaacc gccttggaac
     5101 cttgtggaac cgccttggaa ccgcattgga accttaaaaa atcttttaaa atcaacgatg
     5161 gaaccttgga acctattttt taaaaagagt aatcagggaa attgagagag ctttcgttat
     5221 tggagagaga aggttagaaa atcaggttcc aaaggttcca accagcaatg gcgcgggttt
     5281 aagaaggttc cagtgaggtt ccagcgaggt tccagcgagg ttccaagcca taaaaaaacc
     5341 cgccgaagcg ggttattgtt caatttttgt accggaaccg gtggacgttg tcgggcatcg
     5401 agccggacac gaacgggatg agcaccccct tgccttcagc agcgccacca acatcgcggt
     5461 attttttcgc gcagccgatg atcagatcgc ggtcatgcat caggcgagca cggcgcagcg
     5521 gttcgctttt gtccacgtac cagtcgagga acttacccca gcatccgaca ggagagcgga
     5581 gaaccaaacc agcctttgat tcgaaggtga ggaagtccgg gtgcccatca gcaacacgcc
     5641 catggacgaa cgccacccac tcagcccgaa tttcgtcata cgcgtcggtg atcagcccgg
     5701 cagcattcgc caacccatgc gcagtggtga aagcagccag aacatggtcg gcatcgcggc
     5761 gagcggtctc aaagccctta gcctgatagg acgccacccg ggcattcagc tcggcggcaa
     5821 agaacgacgt caggctgtcg acatagcgtc ccttgtcgga caggaacagc gggcgcgtct
     5881 tgatgtcgcc ctgcagatcg agcatagaaa agcggttcgc cacctgcggg tcaattccgt
     5941 cggagtcgga cagtccgggg atcgggtcgg cactggtgaa aattttcatg tacaggttca
     6001 cgcactgcgt cgtgaggtac ttcggcgtca gagccagcga ctcctcaaga cgaaagtgct
     6061 ttttcgtcac cctggtgcat tcgttgacga tcagcgccag cgcccccatg aagtgactag
     6121 ccgttacacc ggagttggca ccgctcagga tgttgagcag ctccccctcg gacagctcca
     6181 ccgtcgctcc caggcgtgac agaacgccgc cagcaccaaa cagcagcccc ttaccccaat
     6241 cggactgcgc acggaaaaac aggtaggcat tcttacggct tgaggcaaag cgggctgcaa
     6301 ccagcgcgtc gaggaactca tggcattgag ggaaatgaac cagccaatca gccaccacgt
     6361 cactgtcggc attagccacc ggatagggag catgaggacg gacgaacgtc acagtttcac
     6421 ccaccagttc gacgcgagga gccgtggcga acatgtccac gtcgaggcga acgactgcca
     6481 cctgacggcg cagcatgatg aactcagcca ccgcctgacg aacagcccga gccatatcga
     6541 tacacgcctt ggttaccttg tcgtcggtct tgattttgag gtcagtcagg cgggcgataa
     6601 acgcatcggt gcggtgccag aacttgccgc aggagtcctc cagcagcttc attccgtcgc
     6661 ccttatcgaa ctcggtcagg gagccattta ggacagagag gaaccgcccc ttgccagcgt
     6721 gccagacggc agtgccgacg gcagatttga tagcttttac attcaggcca tacagctcga
     6781 attgagcgcg gtagtcacca gaccagtccg cagacaccga caagacagaa aacagcgcat
     6841 cgcccaattt tagcgacagc ggtgacggtg cagcggtgat cggcgttacg ttgtcggcag
     6901 cagcgccgga attttcttca tggtttgaca attttacgtc ctcaaaattg aacagccaat
     6961 ctcaaggagc cttacaacgg cgggacttta cgaggaaaac accctttgca cgcgtcatta
     7021 tgatcgcgta taattcacgc tggcttcaca cccaacatga gttattatgt gttttccacg
     7081 gtgaccgccg tgaaagctcc aaactcaaaa cagctttgaa gaaaacccgt ctccccaagg
     7141 cgggtttttc tttttcagcc gcgccatcat accgttacac ccaccccctg cgccagtaca
     7201 tagcgatccc gcagcgataa acgcgatccc gcgccgatct tagagccgcg cagcctccgc
     7261 gacttatcca cgggccagcc ccgccgcaaa atacttgtcc acagaaactg tccacaggca
     7321 cccaaggcga cgccacgccg tcgcccctca gcgcacgaca attgcactac gtcaaattcg
     7381 acgcgcacga caaaacgcat tacactccgc aagacaaaca gcacgacaaa acgcacgaca
     7441 cggagaggcc agcccatgcg agagaaaacc agaaccctaa ccgttcgact gcccgtcagc
     7501 ctgatcgagc gcatcgagca gcgagcggag aaaaacaaag taacccgcac agatgccgtc
     7561 atagaggcgc ttgtcgccgg acttggcacg aagccggaag ggatggacac caaacttttg
     7621 accgccatcg aggcgctgcg gtcagaggtc agggcgctgg ccggaggcgg gaaagatgca
     7681 cgacagtctg caccacaaac agcacgacaa agcgcaccac aaaacgcacg acagatagtc
     7741 ccccggggag ccgatccccg cttccaggcc gtcagcgatg aggctgacca gctcatcgtc
     7801 agcatgaccg gggagaacgc caccctgccc gcgatcgccc aggcgctgaa cgcagccggg
     7861 cacaccaccg ccaccgggca ggagtggaat accagccgcg tccgggacta ccgccacaag
     7921 ctgcgcgagc gcggcctgat ttgacgcaga ggggcgacgg ctcacgccgc cgccgcatcc
     7981 agccactgca gcatcgccgc cctgatcagc tccacgtcgg cctcgtccat ggtcagcagc
     8041 accatgccat ccacaggagg caacccggcg aacgccgcgc agccggtcac gttgaacgac
     8101 tccaccatca gcgcggtggc cacagcctcc ctttcgaccg cgcacagcca gatgtagtta
     8161 cgctcacgcg acgccggtag cttcacctgc agcgcacggt ttgcacgatc ggtaaacgca
     8221 gcggtacccg cccaggtgta gtcgaacggc gggcgttcga gcaccacaga gccctcctgc
     8281 gtctgctggc gcggcagcgg ggagaagttc ggcagcgagc cgtgagcaaa cccgtcgagc
     8341 gaccaacccc ccatctcaga agcgatcacc tcaagggcgc gagccgtcgc ggcagcgcgt
     8401 aattcagtct ttgatttcat gtgccctcct tacgggcgaa aaaaagcccg acaccaaaac
     8461 ggtgccgggc ttgagtgtgc tgctggaaac caaaccagct gcaggatagc gtcacgaccg
     8521 cagcggcggt gcgtcgcttt aatccgtcta tgcaaaaaag cgtcgatagt gtctcgaaag
     8581 tcggtcgcaa aatccggggc agcggcagca ggaggtgtgt cccgaaaggc gcaccattcg
     8641 gacacgtcaa tcaacgccgc tcaagccggg catgacgaac cgcataccgg agcagctcac
     8701 gtgcccgatc ttccccttcc tcaaccagca cgtcgaacca gtcgaggtta tcccggggag
     8761 tgaaccgatc gaggccgtcg aacgacacaa ccgccgcgcc tggaattgtc gggaccggct
     8821 ctcgctcatc gccataggcc gctttatgcg ccaggcggta cgcttcgccg tgatccggta
     8881 ccagcatcac atcaacatct ggccagaacc gacgcagcga cgccacgcag ctgccgaacc
     8941 cggcgatcga gaacgcagcg cagaccgcca ccttgccacg gctcaggtgg tgcagggcga
     9001 acgccgtagc gaacccctca cagacgaaca gaggcgagta gggcgcgtgc ggggagccgt
     9061 cgagcacgtc catgaacccc ggcatcgggt agaagccgcc ctgcttgcgg gtacccgccg
     9121 gagagaactt cgagccagtg agctggaaac cctcagccga gcaacgcccg cagatggcct
     9181 ccaggccgtc aacatacagc tgacgggcgg cgtcgaactt gtaaaccgga atcaggaggc
     9241 aatcagacca gtaagcacgc ggattgaacg atcgaaacat cccgccaaca ccaagatctg
     9301 tgctgagggc gggcagctgg acgccacccg cagctgccag agcggtcttt ttgcgccagt
     9361 atggggtaga cgcaggatcc cctcccttcg ccagcagctt gaccgcagca cgcgcagcag
     9421 catcaccctt cttgtcgcgt tcagcctttg ccttcgctgt ggcctcacgg gacaaccgca
     9481 gccgttcagc gcgatcgacg tcggactgga cagaagcgcg accgttgcgc cattcgacgc
     9541 cggagaaatt gaggaaagcc tgagcggcga atgtatccag gtcgcctttt aaaaacgacc
     9601 tgtactgctg gcgatggcga ttctcgcctc gcccatgtcg agtggaccag gcctcgatag
     9661 ctgccagccc gtcctcgccg cagagagacg cccagcaacc aagagccagg cagagacgca
     9721 cccattcgtc tcgatcagcc ggagcacccg cagccgatac ggcacgccag aacaggcccg
     9781 gatcctcttt cagatgggta gtgtcgaaat caacgaacgc catcagcagc cctccccgga
     9841 cgacgctggt cggcaacgaa gcgtttgatc tcgggattgc cagccagcca gtaatgcccg
     9901 ccacggaggg agcgccctgc cggagtcggc acgatccccc gcgaacttag ccgcgagagc
     9961 gtgcgcagcg acacgttcag cagcgcagcg aggtcagcgg tatagattcg ttttttcatt
    10021 gggagtacca gataggtgcc cccctcagct taacgggcat cggggggcgg gagtgacgat
    10081 tatcgataaa gccgcccttg aggacttacc gcatagcggc aggacagcag cccttctcgc
    10141 ttactcgcac ctgccaagga cgaagcgcgc atcctcgttt acaaccccgc ctcgttgggg
    10201 aggagggaaa gcggcaccgg gcgatccggt ttcatgtttc ccaactgctc gtcaggaatc
    10261 agtaagacaa gcgtaacaca attaacgcca aagtggcgtt ttttgtgaga tactaaaaaa
    10321 gacgcacagc cagtgctggc gctgtctggc gaccatttcg acgcaggggg agcagtgaac
    10381 gacgataacg acacgaacga atcgacgcag ccgaacccgg cagcacttga acgcgtaagc
    10441 gcgactgaaa aattccccga gcagctcccc ggcgttttgt tcgtcacctg ccccggcggg
    10501 tgctccctcc gggtcgaggc atgccgacag atttatgagc agagccacct cgatcgcacg
    10561 attgaaaacc cccacctcga gtgccaccgg tgccccactg gcctcgcgct ttgggtcggt
    10621 accgaacagc cccctgacgc accactgacc accccggcag acacccgtaa gctctgcccc
    10681 cgttgcagcc ggaaagacaa ccgcctgatc gcccccaata agcccggttc gagcctgtgc
    10741 gtctcctgct ggaaccgcga acgcgaagcc accatcggag ccaacgcacg cgggagagtc
    10801 aaccgcaccc caccgttcgt ctcgccgttc ctgatcggca tcggcgatgg ctggcaggtc
    10861 tggtacggca tccaccaggg cgaggcgctc gtccgcgcca tgcgagccaa cccgggcgca
    10921 cggttccacg atcgccgacc aggcccggtc gggcaaacac gccgcgacgg tttcgtgtac
    10981 tactgccacg aacaccgcga gcccctgcgc tggaacggtg gcgacgggca cgccggagag
    11041 gtggagcttg cttgtcccag gtgccagcca tgggcggcac ccctcagcgt cgcccccacg
    11101 tttcagacgg tcagcttcca gacaccgcat gacgccgcgc accagtaccg gaacgccacc
    11161 ctgccagaca acgcggtctg gacagcgtcg gtctgctccg attgcaaagc cgcgcccctg
    11221 gtgatcagca aggcaccggt caagggcaag ccgcgacggg agatcgtcgt ctcctgggca
    11281 ctgttgcaaa gttagcgatg aggcagcctt ttgtcttatt caaaggcctt acatttcaaa
    11341 aactctgctt accaggcgca tttcgcccag gggatcacca taataaaatg ctgaggcctg
    11401 gcctttgcgt agtgcacgca tcacctcaat acctttgatg gtggcgtaag ccgtcttcat
    11461 ggatttaaat cccagcgtgg cgccgattat ccgtttcagt ttgccatgat cgcattcaat
    11521 cacgttgttc cggtacttaa tctgtcggtg ttcaacgtca gacgggcacc ggccttcgcg
    11581 tttgagcaga gcaagcgcgc gaccataggc gggcgcttta tccgtgttga tgaatcgcgg
    11641 gatctgccac ttcttcacgt tgttgaggat tttacccaga aaccggtatg cagctttgct
    11701 gttacgacgg gaggagagat aaaaatcgac agtgcggccc cggctgtcga cggcccggta
    11761 cagatacgcc cagcggccat tgaccttcac gtaggtttca tccatgtgcc acgggcaaag
    11821 atcggaaggg ttacgccagt accagcgcag ccgtttttcc atttcaggcg cataacgctg
    11881 aacccagcgg taaatcgtgg agtgatcgac attcactccg cgttcagcca gcatctcctg
    11941 cagctcacgg taactgatgc cgtatttgca gtaccagcgt acggcccaca gaatgatgtc
    12001 acgctgaaaa tgccggcctt tgaatgggtt catgtgcagc tccatcagca aaaggggatg
    12061 ataagtttat caccaccgac tatttgcaac agtgccagtc ggcgaatatc ttgaacagga
    12121 ctactatcgg caacttgacg aactatccgt caacacgcct taacttaaac aaaataccct
    12181 ccgccaaacc cggggcggtt cacttcttcg acgagaaaaa ccatgcaaaa cattctgatg
    12241 aatctggcgt tttatctcct ggttgttgct gccggcgcca gcttttccct gcaacaggcg
    12301 gccaacaacc atcttcgcgc agaactgctt tcaccatggt gggcaggttt catcagttat
    12361 gtcggcggtt ccctggccat gctggtcatg gcgctggttt gtcgcggccc tggcctgtca
    12421 tgggatatgc tttcgcgtac cagcccgttt tcatggacag gcggtatttt aggggcgatt
    12481 tacattgcca cggccatttt catgatcccg aaactgggag caacaacggt tctggcattg
    12541 atggtggtcg ggcaaatgct gctttccctg atgctcgatc actatggcct tcttggtatc
    12601 ccggtttatc ctgtcaccgg tatccgcctg ctgggcgttg tcctgcttgt tcttggggta
    12661 gtgctgatca gatggcaggc ataagatcaa gggattcgaa gtcatgcgag ccctgcgcaa
    12721 aggacaggct cgcccctggt gcctgcagcc cggcatcagg ggcgaggtgc gccttgtgga
    12781 gagagctttt ggcattgggc cctcggcgct gacggaggcc atgggcatgc tcaaccacca
    12841 tttcgcagca gccgcctgat cggcgcagag cgacagccta cctctgactg ccgccaatct
    12901 ttgcaacaga gcc
//
