LOCUS       NZ_CP085299             5120 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Enterococcus faecalis strain EFS108 plasmid pEFS108_5, complete
            sequence.
ACCESSION   NZ_CP085299
VERSION     NZ_CP085299.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22349651
            Assembly: GCF_020616935.1
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecalis
  ORGANISM  Enterococcus faecalis
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Kim,H.-Y. and Kim,E.
  TITLE     Complete genome sequence of Enterococcus faecalis EFS108
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Kim,H.-Y. and Kim,E.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2021) Department of Food Science and
            Biotechnology, Kyung Hee University, 1732, Deogyeong-daero,
            Yongin-si 17104, South Korea
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: SMRTlink v. 10.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 31318.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:02:48
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,008
            CDSs (total)                      :: 2,931
            Genes (coding)                    :: 2,896
            CDSs (with protein)               :: 2,896
            Genes (RNA)                       :: 77
            rRNAs                             :: 4, 4, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 4, 4, 4 (5S, 16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 35
            CDSs (without protein)            :: 35
            Pseudo Genes (ambiguous residues) :: 0 of 35
            Pseudo Genes (frameshifted)       :: 11 of 35
            Pseudo Genes (incomplete)         :: 19 of 35
            Pseudo Genes (internal stop)      :: 5 of 35
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085299.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Enterococcus faecalis"
                     /mol_type="genomic DNA"
                     /strain="EFS108"
                     /isolation_source="pig"
                     /db_xref="taxon:1351"
                     /plasmid="pEFS108_5"
                     /geo_loc_name="South Korea"
                     /lat_lon="37 N 127 E"
                     /collection_date="2018"
                     /collected_by="Kyung Hee University"
     gene            complement(join(4597..5120,1..1156))
                     /gene="mobV"
                     /locus_tag="LIT97_RS14950"
                     /old_locus_tag="LIT97_14950"
     CDS             complement(join(4597..5120,1..1156))
                     /gene="mobV"
                     /locus_tag="LIT97_RS14950"
                     /old_locus_tag="LIT97_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401964.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_010717414.1"
                     /translation="MSKIVARMEKMKDGNLSGIQRHNQRETNNHSNPDIDIEKSHLNYD
                     LVNPGSINYREKIKQIIESQRISKRAVRKDAVLVNEWIITSDTAFFQENTDTQAFFTDV
                     VAYFSDRCGRQNVAYATVHLDETTPHMHLGIVPMYEGRLSSKQVFSRQNLLEIQEELPT
                     YLQERGYAIERGLRGSPQKHLSVKEYKDVQKELQVSKQELANQEQILAEKERELHSYEQ
                     AIFRIDRLLEEKNEEVKGKVQAMNQLETKRNEIINSLQTTVLPDLANVKEKKFSLNGLK
                     YVLSASDFSAIKSITKDFETLRTQNKHLKIENNNLAQKNKELTQVNHEQELQITELAYE
                     LTLKKEQLTAFEEKQLNYDQSEQIKQELDTTTQTVSVLETENKRLKDKITDLTYDFSEL
                     NEKYQEIKLKFDGLTTYLHERLGHAKETILFTIQQGVKYLQNAPKLKKSLERYSKDRTY
                     LIDEKGTLFLPPDTGSKGVTMSLNPHFYPLKAPFYNRETGCYEFVGEYMANGRSRTNTL
                     QLSEEQVLEKKAFTFEQSTDKKAWQQSKKYSRDMSKGRGLSR"
                     /name="mobV"
     gene            complement(1933..2229)
                     /locus_tag="LIT97_RS14955"
                     /old_locus_tag="LIT97_14955"
     CDS             complement(1933..2229)
                     /locus_tag="LIT97_RS14955"
                     /old_locus_tag="LIT97_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002404941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002360723.1"
                     /translation="MGLMTGYNMTKEEYEVTQAVLERLDKFESELEALLLDLEPLDLSR
                     LDTVVRDETLRMFNYLSRSYILLIDTVAYKVTLEEDISCGIVKIDGQSLDEEE"
                     /name="hypothetical protein"
     gene            complement(2356..3078)
                     /locus_tag="LIT97_RS14960"
                     /old_locus_tag="LIT97_14960"
     CDS             complement(2356..3078)
                     /locus_tag="LIT97_RS14960"
                     /old_locus_tag="LIT97_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360724.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002360724.1"
                     /translation="MSDKSELAVKYQNELNLVPLKNFNAKQMDLFFALCARMKDKGVEN
                     IRFSFEELKELSDYKMTATKAFVADLEKLYKDMLNLSYRTENDDEIEYFVLFNGFKIDK
                     KQKFVEVRVNQDLDYIINGLTTEFSRFELSAFTSIRSTYAKTLFRLLMQYRSTGYYVVS
                     IEDFRELLDIPEYYQMGNIDQKVLKPAMKELHNYFENLEVTKIKAKKGNKIAKLEFTFT
                     GLKTNKPSVTMHDWVNGE"
                     /name="replication initiation protein"
     gene            3703..3789
                     /locus_tag="LIT97_RS14965"
                     /old_locus_tag="LIT97_14965"
     CDS             3703..3789
                     /locus_tag="LIT97_RS14965"
                     /old_locus_tag="LIT97_14965"
                     /inference="COORDINATES: protein motif:HMM:NF033608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="WP_107196852.1"
                     /translation="MIESLISLIIAPLFVGLVLLLVDRWLDD"
                     /name="type I toxin-antitoxin system Fst family toxin"
     gene            complement(4308..4508)
                     /locus_tag="LIT97_RS14970"
                     /old_locus_tag="LIT97_14970"
     CDS             complement(4308..4508)
                     /locus_tag="LIT97_RS14970"
                     /old_locus_tag="LIT97_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002364281.1"
                     /translation="MKKLLVLILGVSFISVALSSTTEASAREWDSIECQPGFYWKKGGW
                     TAGGYHKAGGFCIPMHTRWTK"
                     /name="hypothetical protein"
     Typing          1442..1701
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="80.0"
                     /coverage="81.0"
                     /evalue="1.95e-45"
     Typing          complement(2357..3078)
                     /name="rep_cluster_2301"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3..1177)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="0.0"
     Typing          complement(4601..5118)
                     /name="MOBV"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="96.0"
                     /evalue="5.6400000000000025e-109"
ORIGIN
        1 ttaaccgctt attctctgtt tctaacacag aaactgtttg tgttgttgta tcaagttctt
       61 gtttgatttg ttcgctctgg tcatagttta gttgtttttc ttcaaacgct gttaattgtt
      121 cttttttcag ggttaattca taggctaatt ccgttatttg taattcttgt tcatgattta
      181 cttgtgtcaa ttctttattt ttctgtgcca agttattgtt ttcgatttta aggtgcttat
      241 tttgcgttct aagcgtctcg aaatctttcg taatactttt tatcgccgaa aagtcagaag
      301 cgcttaaaac gtattttaaa ccgtttaaac taaatttctt ttctttgaca tttgctaaat
      361 caggtaaaac ggttgtttgt agagagttaa tgatttcgtt tcgcttcgtt tccagttggt
      421 tcattgcttg gactttgccc tttacttctt cgttcttctc ttcaagaagg cggtcaatac
      481 ggaagattgc ctgttcataa gaatggagtt ctctctcttt ttctgccaga atttgttcct
      541 gattagcgag ttcctgctta gaaacttgta gctccttttg aacgtctttg tattctttga
      601 cagataaatg cttttgcgga ctcccacgga gtccacgctc aatagcatag cctcgttctt
      661 gcaaataggt tggcaattct tcttgaatct ctaacaaatt ttgccgacta aacacctgtt
      721 tactgctcaa tcgcccttcg tacataggca caattcctaa gtgcatatgg ggcgtggttt
      781 cgtctaaatg taccgtggca taggcgacat tttgtcgacc gcagcgatca gagaaatatg
      841 cgacaacatc ggtaaaaaat gcttgtgtgt ctgtattctc ttgaaaaaag gcggtatcac
      901 tagtgattat ccattcgttc acaaggactg cgtcttttct aaccgctcgt ttactgattc
      961 gttggctctc aatgatttgt ttgattttct cccgataatt gattgaacca ggattgacta
     1021 agtcataatt caagtgagat ttctcaatat caatatcagg attggaatga ttattggttt
     1081 ctcgttgatt atgtcgttgg ataccactta aatttccgtc tttcattttt tccatacgtg
     1141 caacaatttt tgacatgagc gctcctcccc ctttttttct tgtgtcgttc ggtgtctttg
     1201 gatttcggca gtacttccaa gtaaagtata acgcactata ctttacttcg taaaggtgcg
     1261 ctctgccgag ggctgtcggc tagtactcga aaggtcagcc tttaggcttc ccaatctcgc
     1321 acttagccga ttgcctacgg tcaaactttc catttctgct aaggcagaaa ctccaaattt
     1381 ccctccgtca gaaaaccgtt taaataacga ctgcctgaaa ttagtgaacg tgctagcttt
     1441 ctgctagccg gtgctagtaa tgtgctaatt tggtgctaaa aacctgctag tcactgctag
     1501 gaatctgcta gtttgggtgc tagaaacctg ctattgagtg ctaaaaatgt gctagtttac
     1561 ctgaaagtac ctgctagtaa agtgctagcg aagaacgaaa gtgtacccca cgggaaagcc
     1621 ttgcactgca aggcggggta ccttgtgtgc cccccagttc cccttatgct cttccgaaac
     1681 acaggaagaa attaagcgaa tttcaacagt aaaagcaaaa aataacagca gtgggattgg
     1741 gggaacccca aaaatttgaa cgactcctac cagtattttt ggtaggagtc gttcaaaata
     1801 tcgcaacatg gtaccatgtt gctccgcttg caaaaagaaa gcctacccca aaatggaagt
     1861 aggctaatct tgatattttt cgagtgttat cccaaatagt caacattttt ttataacaag
     1921 caagggtagt ttttattctt cttcgtctag agattgaccg tcaatcttca caatcccaca
     1981 ggaaatatct tcttctagcg taactttgta ggctaccgta tcaataagta gaatgtatga
     2041 ccgacttaga taattaaaca ttcttagcgt ttcgtctcga actacagtat ctaatcgtga
     2101 taagtcaagt ggctccaaat caagcaatag cgcttctagt tcactctcaa acttatctag
     2161 tcgctccagt acagcctgtg tgacttcgta ctcctctttt gtcatgttgt atcctgtcat
     2221 taatcccata agtaggacct ccaaaataat ttattttaga agtcctcacg caaatatgct
     2281 aaaataatct agcagtccgg aagattgcgt gaggaacttg ctaacttgct taatgtgagg
     2341 gggcaagttc tttttttatt ctccgtttac ccaatcgtgc atggttacgc ttggtttgtt
     2401 ggttttcaga ccagtaaacg taaattctag tttggcaatc ttattgcctt ttttggcttt
     2461 gatttttgtt acttctaaat tctcaaaata gttatggagt tctttcattg ctggttttaa
     2521 aaccttttgg tcaatgttac ccatttgata atattcgggt atgtctaaca attctctgaa
     2581 gtcttcaata ctgactacat aataccctgt tgaacgatat tgcattaaca aacgaaacaa
     2641 tgttttagca tatgttgagc gtattgaagt aaaagcggac agctcaaacc tactaaactc
     2701 tgttgttaaa ccatttatta tatagtctaa atcttgatta actcgaactt caacaaactt
     2761 ttgttttttg tcgattttaa atccattgaa taaaacaaaa tactctattt cgtcatcgtt
     2821 ttctgtacga taagaaaggt tcagcatatc tttatacaac ttttctaagt ctgctacaaa
     2881 ggcttttgta gcagtcattt tgtagtcact caattccttt aattcctcga aggagaaacg
     2941 aatattctct actcctttat ctttcattcg tgcgcataac gcaaagaaca gatccatttg
     3001 ttttgcatta aagtttttaa gtggcacaag atttaactca ttttgatatt taacagctaa
     3061 ttccgattta tcactcattc ctttcgcccc ctcacaattc gaatattaaa acaaacaacc
     3121 aactttgtca acagttggtt gtttgtttta atattatgaa aataaaacag ataaaaagtt
     3181 gttggtagtc agataaaaag ttgttggtag tcagataaaa agttgttggt agtcagataa
     3241 aaagttgttg gtaccatctg ctcttcctta gagccacgtg gcacacagcc cgcctaaaaa
     3301 ggttttaaaa atatatataa aatatatata aaagaaacgc gtgcgcgcgc ggaaatttcc
     3361 cgcccaaaat taacctcttt tttctacaaa caggtccgcg atatttacaa aactggcact
     3421 aaaaaatcaa aaaaggagtg agcgaaaatc ccgctcacat tttgcttgca gcgttcactt
     3481 cgttcactcg ctaaaatcaa aatcaatacc gttgacaagg taaatcattt ggttaggctc
     3541 tatttgcttg tctgaagatc tttaagacat gtttagttgt tttagggtat cttggttagg
     3601 attgcgcctt gcaggcggtc agggcaagcc ctaacaaccc ccacaagaaa ttcttcttgt
     3661 gatatacttg ttttggcaac tgccccgaaa gggggtgtaa acatgattga aagcttaatt
     3721 tcgttgatta tcgccccact ttttgtgggt ttagtacttt tgctagttga tcgttggcta
     3781 gacgattagc agataatcta tgcagttgct tttctagctc caacgctgaa atcgcaacag
     3841 tgtaaaaaag ccctcctact gagaatagga gggctttttg gtgtaaacat gattttgctt
     3901 aatttcgctt cgcacttaca caataacatt ttttttctgt attttcaaac atcagattag
     3961 attacgcaaa acgtcaatta gagaaatacc atatcgaaat agttagtatt aaacatagta
     4021 atactatgag gatagacaat attttacttt ttggactatc ttcaaagctt ttattttctg
     4081 gtctgtaagt gggattcact actttcgcgt tgtaatccat tgtacttccc aaatatactt
     4141 ctgaaggaat taaaaaaaat aataaaattc cgtaaaaaat aattatcaat atgggcaaat
     4201 caatattaac taaaaaagaa gttatatagc ttaaaactaa aggaatagta atataaaata
     4261 tattttttat tagatatttt ttcatgaaat aacctcaaat ttagcaacta ttttgtccat
     4321 cttgtatgca ttgggataca aaatccaccg gctttatgat atcctcctgc agtccacccg
     4381 ccttttttcc aataaaatcc tggctgacac tctatagaat cccactctct agcacttgct
     4441 tctgtagttg aacttaaagc aacagagata aaagacaccc ctaaaattag aactaacaac
     4501 tttttcataa tgcttacctc actttttttt aatttttatt aatatatttt aatttaactg
     4561 tttttatgtg tcaataattt atcaattaaa ttatttttaa cgagataaac ctcgtccttt
     4621 tgacatatct cttgaatatt ttttactctg ttgccaagcc tttttatctg tcgactgttc
     4681 aaacgtaaaa gctttcttct ctagcacttg ttcttcagac agttgtaaag tattcgttcg
     4741 agaacgtcca ttagccatat attcgccgac aaactcataa caccctgttt ctctgttgta
     4801 aaacggggct tttagggggt agaaatgtgg gtttaaggac attgttaccc cttttgaccc
     4861 cgtatctggt ggtaaaaaca atgtcccttt ttcgtcaatg aggtaagttc tatccttgga
     4921 atatcgctct agggattttt ttaatttagg cgcgttctga aggtacttca ctccttgctg
     4981 aatcgtaaat aaaatggttt ctttagcatg acctagtctt tcgtgaaggt aagtagttaa
     5041 cccgtcaaac tttaatttta tttcttgata tttttcattt agttcagaaa agtcgtaggt
     5101 taagtcagta attttatctt
//
