LOCUS       NZ_CP085191             2088 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Escherichia coli strain EC195 plasmid pEC195-2k, complete sequence.
ACCESSION   NZ_CP085191
VERSION     NZ_CP085191.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22242657
            Assembly: GCF_020616495.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Lin,Q.
  TITLE     Characterization of mcr-1-positive Escherichia coli from Clinical
            Samples in Zhejiang
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Qian,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:07:20
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,154
            CDSs (total)                      :: 5,038
            Genes (coding)                    :: 4,754
            CDSs (with protein)               :: 4,754
            Genes (RNA)                       :: 116
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 284
            CDSs (without protein)            :: 284
            Pseudo Genes (ambiguous residues) :: 0 of 284
            Pseudo Genes (frameshifted)       :: 99 of 284
            Pseudo Genes (incomplete)         :: 179 of 284
            Pseudo Genes (internal stop)      :: 59 of 284
            Pseudo Genes (multiple problems)  :: 46 of 284
            Pseudo Genes (short protein)      :: 4 of 284
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085191.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC195"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC195-2k"
                     /geo_loc_name="China: Wenzhou"
                     /collection_date="2019"
     gene            224..1189
                     /locus_tag="LIN52_RS25665"
                     /old_locus_tag="LIN52_25675"
     CDS             224..1189
                     /locus_tag="LIN52_RS25665"
                     /old_locus_tag="LIN52_25675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_039064478.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLAIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     gene            1193..1459
                     /locus_tag="LIN52_RS25670"
                     /old_locus_tag="LIN52_25680"
     CDS             1193..1459
                     /locus_tag="LIN52_RS25670"
                     /old_locus_tag="LIN52_25680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     Typing          618..849
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 gcccagtaat gacgaggctt tgagtgggtt ttgacaggtc aaagaaaatg gagcagaatt
       61 gaggcgtttt taatcggcgt tggggagtgc gtcaacactc cccaacattt cgaatgtgtc
      121 acctcagcgg caaactctgg tgacatgtac tggctcgcaa tgcacaggta cgtgatgaat
      181 ataccacatc aaatcacagc ctgcccagat cggagcaggc ttaatgtcag aagataaatt
      241 cctttcggac tacagccccc gtgatgcagt ttgggatacc cagcgcacgc ttaccgattc
      301 tgtcgggggt atctaccaga ctgctgctga attcgagcgc tatgcactcc gtatggcctc
      361 ctgtagcggt ttgttacgtt ttggttggtc taccatcatg gaaaccggag aaacgcgcct
      421 acggcttcgt agtgcgcaat tttgccgtgt ccgtcattgc cctgtctgcc agtggagaag
      481 aaccctcatg tggcaagccc gtttttatca ggctctaccg aaaatcgttg tggattaccc
      541 gtcttcccga tggttgtttc tgacgttaac tgtcaggaac tgcgagatag gtgaacttgg
      601 aacagtcctt acagcaatga atgcggcgtt taagcgaatg gaaaagcgaa aggagctatc
      661 acctgttcag gggtggatca gggctacgga ggtgacgcga ggtaaggatg gcagcgcaca
      721 tccgcatttt cactgtctgc tgatggtgca accttcttgg tttaaaggga agaactacgt
      781 taagcacgaa cgttgggtag aactctggcg cgattgcttg cgggtgaact atgagccgaa
      841 tatcgatatt cgggcagtaa aaactaagac aggtgaggtt gtggccaacg ttgccgagca
      901 actgcaaagc gcggttgctg aaacgctgaa atactccgtt aaaccggaag atatggcaaa
      961 cgatcctgag tggtttcttg agctgacgcg gcagcttcac aagcgccgtt ttatctcgac
     1021 cggtggagcg ctaaaaaacg tcctccagtt ggatcgagaa accaatgagg atcttgccat
     1081 tgccgacgat gtaggggatg gcactgatga cgggaagcgg acggcgtttg tctgggattc
     1141 aggtaaacgg cgttacaaac gcgcccctga gaaggataaa tcggattaac gtatgaatat
     1201 taatattgaa tacctgaatg gaaataagac tattggttta ttttttttaa gaagtgaagc
     1261 ggtgattcct gacaggttta aaaaccttat tttgcttatt gatggattaa gttttggcac
     1321 atttggtttt catccgcacg aaggttttga ggatgaatta attttatata ttcagaaaac
     1381 aaacgagagg gtaaaaactc tttttgtgaa aatagattta aacggtatca agtttggatt
     1441 tttaagaacg cattcttagt tctggaaaag agccagcggc aggctgaggt gataggtacg
     1501 agattgcatg caatctctag tgctctgtct atcctgcatt atcctcagca ttatcctcag
     1561 ccttgccaac tcgacaccaa tgcaggatag acaatccgat gtcaaatgtt aacactctgc
     1621 gagtggtaca ttttccccgg attatcgtcc tgagcctgcc gctggctctc tttctaccgc
     1681 ctcgctttgc tcgttgctca acgcctcaca gacacggatt aaaatccgca tccgttcacc
     1741 gttttttaaa gtccgttaaa agcatgatgc catctccgag agttaatctc gtcaaatgct
     1801 aaatcgtggg ggtccccttt ggggttccga tttagtgatt gacgacacca ccgattaaaa
     1861 aacttatgcg gggtggatgg tttcacgaag tgaggccatc cacctgtaag acagggtttt
     1921 gtttttatcc cctgttttgg tgatcgggtg tgtggaaaag gttggggtaa gccgttcggg
     1981 gtgcttgttt tggggggtta aaattgtggt tattttttgc gcaattctcg cgcgtgatcc
     2041 ttgtatttat acttaaggga taaatggcgg gtatgaaata gtggttta
//
