LOCUS       NZ_CP085187             2621 bp    DNA     circular CON 23-NOV-2023
DEFINITION  Escherichia coli strain EC7518 plasmid pEC7518-2k, complete
            sequence.
ACCESSION   NZ_CP085187
VERSION     NZ_CP085187.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22242652
            Assembly: GCF_020616475.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Lin,Q.
  TITLE     Characterization of mcr-1-positive Escherichia coli from Clinical
            Samples in Zhejiang
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Qian,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) Laboratory Medicine, College of Life
            Sciences, Wenzhou Medical University, Wenzhou Medical University,
            Wenzhou, Zhejiang 325000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100x
            Sequencing Technology             :: Oxford Nanopore
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/23/2023 04:12:01
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,082
            CDSs (total)                      :: 4,960
            Genes (coding)                    :: 4,715
            CDSs (with protein)               :: 4,715
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 245
            CDSs (without protein)            :: 245
            Pseudo Genes (ambiguous residues) :: 0 of 245
            Pseudo Genes (frameshifted)       :: 82 of 245
            Pseudo Genes (incomplete)         :: 161 of 245
            Pseudo Genes (internal stop)      :: 48 of 245
            Pseudo Genes (multiple problems)  :: 44 of 245
            Pseudo Genes (short protein)      :: 2 of 245
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085187.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC7518"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC7518-2k"
                     /geo_loc_name="China: Wenzhou"
                     /collection_date="2015"
     gene            complement(274..474)
                     /locus_tag="LIN51_RS25305"
                     /old_locus_tag="LIN51_25305"
     CDS             complement(274..474)
                     /locus_tag="LIN51_RS25305"
                     /old_locus_tag="LIN51_25305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin VbhA family protein"
                     /protein_id="WP_057527577.1"
                     /translation="MLDDRTRQLRVDGFRKAEASLRLEGMDPSGTPLYESVKARILSGE
                     ITYGEGLAEILAHYQKRADSN"
                     /name="antitoxin VbhA family protein"
     gene            complement(1383..2141)
                     /locus_tag="LIN51_RS25310"
                     /old_locus_tag="LIN51_25310"
     CDS             complement(1383..2141)
                     /locus_tag="LIN51_RS25310"
                     /old_locus_tag="LIN51_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_025263533.1"
                     /translation="MTPFWKPMPYYALREKEVLKLFKRNNLSSSIAAIKIYILICLTSN
                     LDENGNYCTSLTYDQLTKYASLSRKLVSNGLKKLYELELLSFEGDKKKKYYLMGVKRTG
                     KVFSETRFKSSQGYWAKLPFAGFVDGSGRITAFESMSNRSPVELNSLKLFIYLLSIRSK
                     DQVSISVTLSKIRSKLDISFQDIMIAIGFMQSIGMLYKVNLGVNTISNYDAAFSINFLV
                     CGWESLEWKPTYLSHDEWKNKMLAKMFPEK"
                     /name="hypothetical protein"
     Typing          526..1146
                     /name="rep_cluster_2324"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.605"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 ggctatgcac acgagtgaat ggtcgggggt ttcgcgcgga agctgtcgcc aaagggggat
       61 ttccggtgat taactcaccc gataacggtg aacgggtgca cagtttactg tgcggtcgtg
      121 aggcgttggc caagccgcca ggcgcggcag gaatatcgcc aaaggggtcg gatttaacga
      181 ggaggaaccg gagtacctgg cgggtgtcgg gcaggagcct gaccaggagg atttggcgtg
      241 ccggcggtac tccaaagaca catcttgtcc gcgtcagttg ctgtcagcgc gcttctggta
      301 atgagccagt atttctgcga ggccttctcc ataggtaatc tcaccggaaa gaatacgtgc
      361 cttaaccgat tcatagagcg gcgttccaga ggggtccatg ccttcaagcc ggagagaagc
      421 ctcggcctta cggaatccgt ctacacgaag ttgtcgtgta cgatcatcga gcatgggttt
      481 tcctttgagc agtcatgcga ataaggcagg cgtaaagtta gctaacttta catgtcctgc
      541 ccggatattg ccgttgtacc gttgtgcagg gaaaagtgga aaacgacaag aaaccgttgc
      601 agccgagtgt gtaatcaggg tggaaacggg tgtaccgagc gatccgagcc ctgttcagca
      661 ttacagacat tacgcttcct cggtcattcg ctcacttcgt tcggtcatga cgctgcggcg
      721 agcgttactt gctcaggcag aaggccatga cggagagtga aagagagctg agacgttgcc
      781 ggttttccat aggcttcgcc ctcctgacga gactcacaga aatcaggcga tttctggtgc
      841 tcaaatcaga agtggcgaaa cccgacagga cttaaagatc cccaccgttt ccgatgggtc
      901 gctccctcgt gcgctctcct gttcccaccc tgaggtttac ccgttgcaga ccgccgtaag
      961 gtggcgtttt ctcattccta cccgtgcaac tgggttcctt gtaggtggtt cgctccaagc
     1021 tgggctgtat gcacgaactc cccgttcaac ccgactaccg cgcctattca ggtaactgta
     1081 ctcttgaatc caaccctgac agacacgaca aagcgccact ggcggtagcc actggtaaca
     1141 ggtttagagg aggagagtct tgaagtttcg ggtgcgataa ggctacactg aaaggacagt
     1201 tttggtgact gtgctcctcc aagccagtta ccgcggttca aaagagcgtc aactcttgcg
     1261 aaccttcgaa aaaaccaccc ggcaaggtgg ttttttcgtt tcaaggctaa gatacgcttt
     1321 aaaatggtta gcggtcaaga agtcaggctt ccttctaagc tcaagattag tcaaaaaaaa
     1381 catcattttt caggaaacat ttttgctaac atcttatttt tccattcatc atgagaaaga
     1441 taagtgggct tccactccag actctcccat ccgcaaacaa gaaaatttat ggaaaaggca
     1501 gcatcgtagt tagatatagt gttaacgccg agattaacct tataaagcat gcctattgac
     1561 tgcatgaaac ctatcgcaat cattatatcc tggaatgaaa tatctaactt tgatcttatc
     1621 ttacttaacg tcacactaat agaaacctga tcttttgatc tgatagataa caaataaata
     1681 aaaagcttta gtgaatttag ctcgactgga cttcgattgc tcattgactc aaatgctgta
     1741 attctaccag aaccatctac aaacccagca aacggcagtt ttgcccagta tccttgtgat
     1801 gacttgaatc tggtttctga aaaaaccttc ccagttctct tcacacccat caaataatat
     1861 tttttctttt tgtcaccctc aaaactcagc aactccaatt catatagttt tttcaaacca
     1921 ttgctgacca acttacggct aagcgaggcg tacttggtca attggtcata agttaaagag
     1981 gtacaatagt tcccattttc atctaagttg ctagttaagc aaattaaaat atatatttta
     2041 atggcagcaa tgcttgaaga taaattattc ctcttgaaaa gttttaaaac ttctttttct
     2101 cgaagagcgt aataaggcat gggcttccaa aagggcgtca tttatcctcc aaaatacatg
     2161 tgtagacaaa tcagcagcat gaccactgac agaatcagcc gcgataaatc catcgagata
     2221 cgagcaatgc gctcaagtaa agctacccag ttttcaggtg acataatcaa tctcccagtg
     2281 gtcatgaatg cgttttttaa aattatgacc gagaacccta aagacttata gtctttaggg
     2341 ttaaatcttt ctagaataag cgcacactaa aaaatcatta ttatcagtac attgtataaa
     2401 aataatagtt catgttaata ccagaatagt gcacgtaatc cattttatag cagcatcatg
     2461 cctgagtgat cttcatatca gagtcctatt taccataaca tcccttgccc gcacccccag
     2521 aaaggccctc agcgcgtttc tgccgcatcc gggccgtttt gggcaccttt ccctgcctgt
     2581 atcgtgaaaa aacgcccctg atgattttgg cgcggctgca t
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