LOCUS       NZ_CP085073             1506 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain P27_L1_05.20 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP085073
VERSION     NZ_CP085073.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22251400
            Assembly: GCF_020541005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Wide distribution of IncF plasmids carrying blaNDM-5 and rmtB in
            Escherichia coli from hospitals in England similar to those found in
            other countries and continents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1506)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: RR20_BC10_Ecol_P27_L1_05.20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 412.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:15:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,929
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 85 of 217
            Pseudo Genes (incomplete)         :: 144 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 54 of 217
            Pseudo Genes (short protein)      :: 2 of 217
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085073.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P27_L1_05.20"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2020-05"
                     /genotype="ST8346"
     gene            1..441
                     /locus_tag="LH371_RS25140"
                     /old_locus_tag="LH371_25140"
     CDS             1..441
                     /locus_tag="LH371_RS25140"
                     /old_locus_tag="LH371_25140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000031238.1"
                     /translation="MSKDVERITQINDQGEIIGGFVAVIRPKQKSAFQRHFTMNQDALR
                     ILAKELTGEQFKVLMLMLADLDYENFIQIAQADIAETLGMQKTNVSRAVRALLDVGVIF
                     EGPKVGRSKTYRLNEQFGWKGTVTNHKKALKNGFSVIQGGRI"
                     /name="hypothetical protein"
     gene            complement(499..1158)
                     /locus_tag="LH371_RS25145"
                     /old_locus_tag="LH371_25145"
     CDS             complement(499..1158)
                     /locus_tag="LH371_RS25145"
                     /old_locus_tag="LH371_25145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rep protein"
                     /protein_id="WP_226908833.1"
                     /translation="MCKCNTVTRDDIPFAIWSGLHPDTPHQCVRAQCGFRFSSASSPTR
                     SLTFGRWGCGERCHLSHVIGDSAVVLSGMSRQIALQTLSEALRRTKRVLNARQGMEGAK
                     NAYRGFQEVRRADTPNQGLSGRQEPRQPPAKPICTALAAIQLVGGRRPSMLQRWGAGVL
                     PRRFPVALKALPEPWQLRRFGDYRAKVANDVNDVILRRLPYPASHRTDMRQKSNAG"
                     /name="rep protein"
     Typing          complement(727..983)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.186"
                     /coverage="98.0"
                     /evalue="3.06e-107"
ORIGIN
        1 atgagtaagg atgtcgagag aatcactcag ataaatgacc agggtgagat tatcggcggc
       61 ttcgttgccg ttatccgccc taaacaaaaa tcagcatttc agaggcattt caccatgaat
      121 caagacgcat tacggatctt ggctaaggaa ttaaccgggg agcaattcaa ggtcttaatg
      181 ctgatgcttg cagatctgga ttacgaaaac ttcattcaga tcgcccaagc agatattgct
      241 gaaactttag ggatgcagaa aacgaatgtt agtcgcgccg ttcgcgcgct actggatgtt
      301 ggggtgatct ttgagggacc taaagttggc agatcaaaaa cataccggct gaatgaacaa
      361 tttggttgga aaggcaccgt aaccaaccac aaaaaggcac tcaaaaacgg ctttagtgtc
      421 attcagggcg gccggattta aagagccagc ggcaggcgga caatcagggc tacgtgttaa
      481 cgttttgacc atgattgtct atcctgcatt gctcttttgc cgcatgtcag ttcgatgcga
      541 tgcaggatag ggcaaacgcc gcaaaatgac gtcattgacg tcattcgcaa cctttgcccg
      601 ataatctcca aaacgtctga gctgccacgg ttccggcagc gccttgagcg caaccggaaa
      661 ccgtctgggg agaaccccag ccccccatcg ctgtaacatc gacggtctac gaccaccaac
      721 cagctgaata gctgccagtg ccgtgcaaat gggctttgca gggggctgac gcggttcctg
      781 gcgacctgaa agcccctggt taggggtatc ggcgcgcctg acctcctgaa accctctgta
      841 agcgtttttc gcgccttcca tgccctgacg ggcatttaaa acccgtttcg tgcgcctgag
      901 agcttctgag agcgtttgaa gagctatctg gcgactcatc cccgaaagaa caacagcgga
      961 atcaccgata acatgcgaga ggtggcaccg ctcgccgcag ccccagcgac cgaacgttag
     1021 tgagcgagtg ggcgaggaag cggaagagaa ccggaaacca cattgagcac gtacgcactg
     1081 atgcggggtg tcggggtgaa gccctgacca gatggcaaac ggaatatcgt cgcgggtgac
     1141 ggtattacat ttgcacatcc tgtcccgatt tctgcggagt ttaacggtga acggacggca
     1201 aaaaacgtgc aggagctgtt gaacgtgaaa acctcgaaat ctggcaaaaa tcacggctga
     1261 cgaggcttgc agaggattaa gccagtatac actccgctat cgttacgtga ctgggtcagg
     1321 gcttcgcccc gacaccccaa agagtagcta ggataaatat ctaatggaat caatgcgtta
     1381 gacgcctaat ataaagatac agaaaggaga ataaaaacat taacaaaatc taagggttac
     1441 agttgacata gttatcaata atgataacac tgttatcatt attgataact caaatagggt
     1501 tcaaag
//
