LOCUS       NZ_CP085072             4073 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain P27_L1_05.20 plasmid unnamed2, complete
            sequence.
ACCESSION   NZ_CP085072
VERSION     NZ_CP085072.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22251400
            Assembly: GCF_020541005.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Wide distribution of IncF plasmids carrying blaNDM-5 and rmtB in
            Escherichia coli from hospitals in England similar to those found in
            other countries and continents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: RR20_BC10_Ecol_P27_L1_05.20
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 412.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:15:17
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,051
            CDSs (total)                      :: 4,929
            Genes (coding)                    :: 4,712
            CDSs (with protein)               :: 4,712
            Genes (RNA)                       :: 122
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 91
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 217
            CDSs (without protein)            :: 217
            Pseudo Genes (ambiguous residues) :: 0 of 217
            Pseudo Genes (frameshifted)       :: 85 of 217
            Pseudo Genes (incomplete)         :: 144 of 217
            Pseudo Genes (internal stop)      :: 46 of 217
            Pseudo Genes (multiple problems)  :: 54 of 217
            Pseudo Genes (short protein)      :: 2 of 217
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085072.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P27_L1_05.20"
                     /isolation_source="rectal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed2"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2020-05"
                     /genotype="ST8346"
     gene            237..938
                     /locus_tag="LH371_RS25125"
                     /old_locus_tag="LH371_25125"
     CDS             237..938
                     /locus_tag="LH371_RS25125"
                     /old_locus_tag="LH371_25125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001281399.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001281395.1"
                     /translation="MSDLIAYKSNALIEASYKLTLQEQRFLLVCISRLKSGEDSPSPDE
                     QKTMTITAAEFHDAFPDMGRQHAEARLKEAIDRLWDRSVIIKNEEKREEFRWVQYRAQY
                     FKGEAKVDITFSDAIMPYLTQLKGQFTRVVVKNVSSLSSTYSIRIYELLQQFRSTGDRT
                     IAIDDFRTMLAVEDKYQQFKDLNKMIIKPAIAELNEKSDLVVTVETIKKGRTVVALHFR
                     FKEDKQIKMTI"
                     /name="replication initiation protein"
     gene            complement(1196..3343)
                     /gene="mobQ"
                     /locus_tag="LH371_RS25130"
                     /old_locus_tag="LH371_25130"
     CDS             complement(1196..3343)
                     /gene="mobQ"
                     /locus_tag="LH371_RS25130"
                     /old_locus_tag="LH371_25130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_001029354.1"
                     /translation="MAIYHLSMKIISRSSGYSAVASAAYRSGSLMLDERTGLTHDYTRK
                     SGVAEAVILTPATAPAWCTNRAELWNAVEKAERRKNSQLAREIELAIPHELPQDAARET
                     VLAFVRENFVSQGMIADVAFHHMDKTNPHAHIMLTTRAIGPAGFSGKVRDWNDRTHAET
                     WRASWADHANRALANAGYQEEIDHRSYERQGLEKTPGIHLGKSACAMETRGIETERGEQ
                     NRLINRLNLEIQISRTELRNGGLTEPARRVADLLNIGLPDNATSYTLRDIIEALPKDSN
                     SAWKLTSNAMSMMADMQATRRRWEELNTERKEAMSHAASLKKSSPFSSGFSRIPLMQWA
                     APEYRSEQGKIQSLSQQMEKLRQHYRKVEKQDIPASQMAFEKQWQQWGESGLAALRAQL
                     LKREEELRRKEQEDTERSRAEQLRKNDNAVMDSGVLRAVVTGYGEAPMPGNGEMTCYLL
                     LHNRNGEYTLWGNELEKYRTRIFESVDLMRDRSGYICDRSEIGQRPPLQRVYSSATFEQ
                     LLTQVCQTWPQHTRNLRQPKTWPESFCLGEDRQPAMPSPAARKVDFMQGRLPPTLMPVM
                     SSVDRETRQLQLLLVMGVDDSLGGVIRLNGTLYPAFAVPSADNRHLVISALTDKGLRYA
                     GYGVAVNHDADSHISPAPELMQFHLKTREAPLFAAVNTPEKQPDHLFRSLGFNRTWEEW
                     QREEDARTHTTERRHDRGWSQ"
                     /name="mobQ"
     gene            3522..3776
                     /gene="mobC"
                     /locus_tag="LH371_RS25135"
                     /old_locus_tag="LH371_25135"
     CDS             3522..3776
                     /gene="mobC"
                     /locus_tag="LH371_RS25135"
                     /old_locus_tag="LH371_25135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase accessory protein MobC"
                     /protein_id="WP_000091746.1"
                     /translation="MSRTLEQKIADAEARLQRLKAKSRSLDTAQKVVVGAALLAKVRKP
                     EEVQLRAWLLQFLKAEVTRQADVTRILPLINELEALPEQ"
                     /name="mobC"
     Typing          complement(2986..3344)
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3345..3521
                     /name="MOBQ"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="5.61e-88"
     Typing          238..938
                     /name="rep_cluster_1778"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1200..3430)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.989"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 aaaccttttt aggtcttgta acaccatgat tttaaagatg ttctggcaag ataagtggag
       61 cttttggggg tgaaaggtgg agcttttggg ggtgaaaggt ggagcttttg ggggtgaaag
      121 gtggagcttt tgggggtgta atccacagaa aaagggggtt gcaaaaaaaa aacttccacc
      181 catatgataa gtggcgtaca gtaaaaaaac gccaccaata gcaacgagca agcgaaatgt
      241 cagatctgat agcttacaaa tcaaacgccc ttatcgaggc aagctacaag ctcactttgc
      301 aggaacagcg cttcctgctg gtctgtatca gccgcctaaa atccggagaa gattcccctt
      361 cgccggatga acaaaaaacc atgaccatca cggctgcaga gtttcatgat gcctttcctg
      421 acatgggacg gcagcacgct gaagctaggc tcaaggaagc gatagatcgc ctctgggaca
      481 ggtcagtaat catcaagaac gaggaaaaac gagaagagtt ccgctgggtg cagtaccggg
      541 cgcagtattt caaaggtgag gcgaaagtag atatcacctt ctccgatgcc atcatgccgt
      601 atctgacaca gctcaagggg cagtttacgc gggtagtcgt gaagaacgtc tccagccttt
      661 ccagcaccta cagcatccgc atttacgagc tgctccagca gttccgcagc accggtgaca
      721 gaacgatagc tatcgatgat ttccgaacca tgcttgccgt tgaagataaa tatcaacaat
      781 tcaaagactt gaataagatg ataatcaagc cagccatcgc cgaactgaac gaaaaaagcg
      841 atctggttgt aactgttgag acgatcaaaa aggggcgcac ggttgtagcc ctgcattttc
      901 gcttcaagga agacaaacag atcaagatga cgatctaaaa tcgattttaa ggcacctaaa
      961 acgctccctg ctgcgttttt tttgtgttag tagtgcgaag gtactattga agtgaaaaat
     1021 tgcgtcaaaa acgctctgat gcagcgaaaa aagacccccc aatttttggg ggcggaaaaa
     1081 caaaactatg cttgcgttat gtgagtgtgc ttcttgcatt catccctgtc gatgaacatc
     1141 gaagaacatc gacgaacata acacgatgtt aaacaaaaca aaatattgtt cacattcact
     1201 gtgaccaacc gcgatcatgt ctcctctcgg tggtgtgtgt tcgtgcatct tcctcgcgct
     1261 gccattcctc ccaggtacga ttaaaaccca gcgagcggaa cagatgatcc ggctgttttt
     1321 ctggtgtgtt gacggcagca aaaagcggtg cctcacgggt tttcagatga aactgcatca
     1381 gttctggtgc aggtgatatg tggctgtctg catcgtgatt tacggcaaca ccataccctg
     1441 cataccgtaa ccccttatcg gtcagcgcac tgatgaccag atgcctgtta tcggctgagg
     1501 gaacggcaaa cgcgggatac agcgtaccat taagccggat aacaccgccc agagagtcat
     1561 ccacgcccat gaccagcaaa agctgaagct ggcgggtttc ccggtcgaca gaactcatga
     1621 ccggcatcag cgtcggtggc agacgtccct gcataaaatc caccttcctt gccgctggtg
     1681 atggcatggc tggctggcgg tcttctccaa gacagaaaga ctccggccat gtcttcggct
     1741 ggcggagatt gcgggtatgt tgcggccatg tctggcaaac ctgcgtcagc agttgctcaa
     1801 aggttgcaga ggaataaaca cgttgcagtg gcggacgttg gccgatttcg cttcggtcac
     1861 agatataacc actcctgtca cgcattaaat caacactctc aaagattctg gtgcggtatt
     1921 tctccagctc gttcccccag agcgtatatt ctccgttccg gttgtgaagc agaaggtaac
     1981 aggtcatttc cccgttacca ggcatgggag cctcgccata ccccgtaacg actgctctca
     2041 gcacccctga atccataacg gcattgtcat ttttcctgag ctgctccgcg cgactacgtt
     2101 cagtatcttc ctgttccttc cggcgcaatt cctcttcacg tttaagcaac tgggctctga
     2161 gagctgcaag gccggattcc ccccactgct gccactgttt ttcaaatgcc atctgactcg
     2221 cgggaatgtc ctgtttctcc accttccggt aatgctgtcg taatttttcc atttgctggc
     2281 tcagagactg gatttttccc tgctcactac ggtattccgg tgccgcccat tgcatcaggg
     2341 gaatacggga aaaaccggac gaaaacggcg atgatttttt caggctggca gcatggctca
     2401 tcgcttcctt tcgttcggtg ttcagctctt cccagcgtct gcgcgtcgcc tgcatatctg
     2461 ccatcatcga catggcatta ctggtgagtt tccatgccga gttactgtcc ttcggcaacg
     2521 cttcgataat gtcacgtagt gtgtacgaag ttgcgttgtc aggaagcccg atattcagaa
     2581 ggtcagccac acggcgggca ggctctgtca gccctccgtt cctgagttcg gtgcgggaaa
     2641 tctgtatttc caggttaagc ctgttaatca gacgattctg ttcgccgcgt tctgtttcga
     2701 taccgcgtgt ttccatcgca caggcagact ttccgaggtg aataccaggc gttttctcaa
     2761 gcccctgtcg ctcgtatgaa cgatggtcta tctcttcctg ataaccggcg ttcgccagtg
     2821 ccctgtttgc atggtcagcc catgatgccc gccacgtctc ggcatgtgtc cggtcgttcc
     2881 agtccctgac ctttccgctg aacccagcag gtccgatagc cctcgtggtc agcatgatgt
     2941 gcgcatgggg atttgtttta tccatgtgat ggaacgccac atcagcaatc atgccctgac
     3001 tgacaaagtt ttctctgaca aaggcgagaa cggtttcccg tgcggcatcc tgcggcagtt
     3061 cgtgggggat ggcgagttca atttcccgcg caagttgtga gttcttccgg cgttcggcct
     3121 tctcaacggc attccagagt tcggcacggt tcgtacacca ggcaggggcg gtggcggggg
     3181 tcagaatcac agcctcagcc acaccgcttt tgcgggtgta gtcgtgagtc agtccggtac
     3241 gctcatcaag catcagtgaa ccggaacgat aggcagcgga agctacggcg ctgtagccgc
     3301 tgcttctgga gataattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     3361 tttgtagttt tctttcagcc ccgcagggcg cacacaatcg aaacggagtg gagattgtat
     3421 aagtgcgcac ttcgtgattt gccgtgcatt atgccagctc agtttttaaa ctaccagtga
     3481 cgcgctaaaa tgcgattttt catcgcgcac ggagttacat catgagcaga acgctcgaac
     3541 agaagattgc tgatgctgag gcgagattac agcgcctgaa ggcgaagagc cggagcctgg
     3601 acacagcgca gaaggttgtt gtgggtgcag cattgctggc aaaggtcaga aagccggagg
     3661 aggtgcagtt gcgtgcctgg ttactccagt tcctgaaggc agaggtgaca cgacaggctg
     3721 atgtgacgcg catactgccg ctgattaacg agctggaggc gctgccagaa cagtgattgt
     3781 atcgtccgct cgccgcagcc ccagcgaccg agcgttagcg agcgagtggg cgaggaagcg
     3841 gaagtgaatc agtcgccagg ttgagcacat acgcacggtt tttcggcagg tgatatgcgc
     3901 tgaacccgca tgaatactgg gtttccacag ggttggaggg ggtgtattcg aaacttgcaa
     3961 ttctgcttta ttgcaacgca ttgtttttat tggttttttg ttttcagcac cccaagttat
     4021 ccacaggctc gccgttaagt ttttaagatc ttatttttag atcttttttt tta
//
