LOCUS       NZ_CP085068             1553 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain P31_YH1_02.21 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP085068
VERSION     NZ_CP085068.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22251350
            Assembly: GCF_020540985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Wide distribution of IncF plasmids carrying blaNDM-5 and rmtB in
            Escherichia coli from hospitals in England similar to those found in
            other countries and continents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: RR20_BC09_Ecol_P31_YH1_02.21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 419.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:15:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,353
            CDSs (total)                      :: 5,226
            Genes (coding)                    :: 5,004
            CDSs (with protein)               :: 5,004
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 81 of 222
            Pseudo Genes (incomplete)         :: 146 of 222
            Pseudo Genes (internal stop)      :: 41 of 222
            Pseudo Genes (multiple problems)  :: 44 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085068.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P31_YH1_02.21"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed4"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2021-02"
                     /genotype="ST405"
     gene            1..477
                     /locus_tag="LH370_RS26680"
                     /old_locus_tag="LH370_26680"
     CDS             1..477
                     /locus_tag="LH370_RS26680"
                     /old_locus_tag="LH370_26680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000469298.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVRE
                     LLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKNAL
                     VRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
                     /name="helix-turn-helix domain-containing protein"
     Typing          1072..1157
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="91.954"
                     /coverage="95.0"
                     /evalue="4.39e-26"
     Typing          complement(1367..1475)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="1.95e-50"
     Typing          complement(709..969)
                     /name="Col(MG828)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.13"
                     /coverage="100.0"
                     /evalue="1.84e-104"
ORIGIN
        1 atgtttattg attcagaaaa acgactgaaa caactttcag atgaggcaaa gaaaaacacc
       61 gaggatctcg aagaagcaaa gaagaattca aggtttacac aggtatcccc aaaaggttgg
      121 gaacgcgttc gagagctgct gaaggatagc caaggcatat cagcactgaa gctgtactca
      181 tttttagcgg agcatatcga tcctacgtgt ggcgctgtcg ttgcggatca gcaattccta
      241 gctgaaaaac ttggagttag cagaagcaca attattcgtt ggctcaatta cttagaatca
      301 aaaaatgcat tagttagaat ccccgttgct ggtaaggttt gtgcgtatgc cctcgatcca
      361 catgaagtct ggaagggata caacactacg aaaaaccatg cagcatttgt cactaaaaca
      421 ctggtcaaca aagacggtga tattcagcgc cgaatcatgg ccatgttttc aaattgagcc
      481 agcggcaggc ggacaatcag gggttacgtg ttaacgttct gaccatgatt gtctatcctg
      541 cattgctctt ttgccgcctc aaaatccctt gcgtgttttt gctccccgat ctccagaacg
      601 accgagccgc cacggttccg gcagcgcctt gagcgcaacc ggaaaccgtc tggggagacc
      661 cccagacccc ccatcgctgt aacaccgacg gtctacgacc acctaccaac cgagtggctg
      721 cttgtaccgt gcaaatgggc tttgcagggg gctggcgcgg ttcctggcga cctgaaagcc
      781 cctggctagg ggtatcggcg cgcctgacct cctgaaacgc tctgtaagcg tttttcgcgc
      841 cttccatgcc ctgacgggca tttaaacccc gcctcgtgcg tctgagagct tctgagcgcg
      901 tttgaagggc tatctgacga ctcatccccg aaagaacaac agcggaatca ccgacagcat
      961 gcgagaggtg gcaccgctcg ccgcagcccc agcgaccgaa cgttagtgag cgagtgggcg
     1021 aggaagcgga agagaactgg aaaccacatt gagcacgtac gcactgatac ggggtgtcgg
     1081 ggcgaagccc tgaccagatg gcaaacggaa tatcgtcgcg ggtgacggta ttacatttgc
     1141 acatcctgtc ccgatttctg cgggggttaa cggtgaacgg acggcaaaaa acgtgcagga
     1201 gctgttgaac gtgaaaacca cgaaatctgg caaaaatcac ggctggcgag gcttgcagag
     1261 gagtaagcca gtatacactc cgctatcgct acgtgactgg gtcagggctt cgccccgaca
     1321 cccccaaagg gcgttgcgtt gcgcgcaaca cccttgccta gaatagatct tttacgccga
     1381 tttgtaagtt gttgtttttt tagtgtgttt tttgtttacc tgtcgcatcc agggtgacac
     1441 aacgttgtcg catcctggat gcgacaacac gcaacaaggt gttgcacaga tgtgtcatga
     1501 tggcttagat ttgaaccaac gttagtgcat gggattttta gagggaaaaa atc
//
