LOCUS       NZ_CP085067             2101 bp    DNA     circular CON 22-NOV-2023
DEFINITION  Escherichia coli strain P31_YH1_02.21 plasmid unnamed3, complete
            sequence.
ACCESSION   NZ_CP085067
VERSION     NZ_CP085067.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22251350
            Assembly: GCF_020540985.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Wide distribution of IncF plasmids carrying blaNDM-5 and rmtB in
            Escherichia coli from hospitals in England similar to those found in
            other countries and continents
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Turton,J.F. and Turton,J.A.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) HCAI, Fungal, AMR, AMU & Sepsis Division,
            United Kingdom Health Security Agency, 61 Colindale Avenue, London,
            London NW9 5EQ, United Kingdom
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 05-OCT-2021
            Assembly Method                   :: Unicycler v. 0.4.9
            Assembly Name                     :: RR20_BC09_Ecol_P31_YH1_02.21
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 419.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/22/2023 14:15:32
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,353
            CDSs (total)                      :: 5,226
            Genes (coding)                    :: 5,004
            CDSs (with protein)               :: 5,004
            Genes (RNA)                       :: 127
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 92
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 222
            CDSs (without protein)            :: 222
            Pseudo Genes (ambiguous residues) :: 0 of 222
            Pseudo Genes (frameshifted)       :: 81 of 222
            Pseudo Genes (incomplete)         :: 146 of 222
            Pseudo Genes (internal stop)      :: 41 of 222
            Pseudo Genes (multiple problems)  :: 44 of 222
            Pseudo Genes (short protein)      :: 4 of 222
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085067.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="P31_YH1_02.21"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="unnamed3"
                     /geo_loc_name="United Kingdom"
                     /collection_date="2021-02"
                     /genotype="ST405"
     gene            complement(441..707)
                     /locus_tag="LH370_RS26670"
                     /old_locus_tag="LH370_26670"
     CDS             complement(441..707)
                     /locus_tag="LH370_RS26670"
                     /old_locus_tag="LH370_26670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001024536.1"
                     /translation="MNINIEYLNGNKTIGLFFLRSEAVIPDRFKNLILLIDGLSFGTFG
                     FHPHEGFEDELILYIQKTNERVKTLFVKIDLNGIKFGFLRTHS"
                     /name="hypothetical protein"
     gene            complement(711..1676)
                     /locus_tag="LH370_RS26675"
                     /old_locus_tag="LH370_26675"
     CDS             complement(711..1676)
                     /locus_tag="LH370_RS26675"
                     /old_locus_tag="LH370_26675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="WP_001287824.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRMA
                     SCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIVVD
                     YPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRGKDG
                     SAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGEVVAN
                     VAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQLDRETN
                     EDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
                     /name="protein rep"
     Typing          complement(1052..1283)
                     /name="Col(BS512)"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="2.05e-118"
ORIGIN
        1 cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat cactaaatcg
       61 gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc ggagatggca
      121 tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat ccgtgtctgt
      181 gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc aggctcagga
      241 cgataatccg gggaaaatgt accactcgca gagtgttaac atttgacatc ggattgtcta
      301 tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat gctgaggata
      361 atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca cctcagcctg
      421 ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt gataccgttt
      481 aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat atataaaatt
      541 aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact taatccatca
      601 ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct taaaaaaaat
      661 aaaccaatag tcttatttcc attcaggtat tcaatattaa tattcatacg ttaatccgat
      721 ttatccttct caggggcgcg tttgtaacgc cgtttacctg aatcccagac aaacgccgtc
      781 cgcttcccgt catcagtgcc atcccctaca tcgtcggcaa tgacaagatc ctcattggtt
      841 tctcgatcca actggaggac gttttttagc gccccaccgg tcgagataaa acggcgcttg
      901 tgaagctgcc gcgtcagctc aagaaaccac tcaggatcgt ttgccatatc ttccggttta
      961 acggagtatt tcagcgtttc agcaaccgcg ctttgcagtt gctcggcaac gttggccaca
     1021 acctcacctg tcttagtttt tactgcccga atatcgatat tcggctcata gttcacccgc
     1081 aagcaatcgc gccagagttc tacccaacgt tcgtgcttaa cgtagttctt ccctttaaac
     1141 caagaaggtt gcaccatcag cagacagtga aaatgcggat gtgcgctgcc atccttacct
     1201 cgcgtcacct ccgtagccct gatccacccc tgaacaggtg atagctcctt tcgcttttcc
     1261 attcgcttaa acgccgcatt cattgctgta aggactgttc caagttcacc tatctcgcag
     1321 ttcctgacag ttaacgtcag aaacaaccat cgggaagacg ggtaatccac aacgattttc
     1381 ggtagagcct gataaaaacg ggcttgccac atgagggttc ttctccactg gcagacaggg
     1441 caatgacgga cacggcaaaa ttgcgcacta cgaagccgta ggcgcgtttc tccggtttcc
     1501 atgatggtag accaaccaaa acgtaacaaa ccgctacagg aggccatacg gagtgcatag
     1561 cgctcgaatt cagcagcagt ctggtagata cccccgacag aatcggtaag cgtgcgctgg
     1621 gtatcccaaa ctgcatcacg ggggctgtag tccgaaagga atttatcttc tgacattaag
     1681 cctgctccga tctgggcagg ctgtgatttg atgtggtata ttcatcacgt acctgtgcat
     1741 tgcgagccag tacatgtcac cagagtttgc cgctgaggtg acacattcga aatgttgggg
     1801 agtgttgacg cactccccaa cgccgattaa aaacgcctca attctgctcc attttctttg
     1861 acctgtcaaa acccactcaa agcctcgtca ttactgggct aaaccactat ttcatatccg
     1921 ccatttatcc cttaagtata aatacaagga tcacgcgcga gaattgcgca aaaaataacc
     1981 acaattttaa ccccccaaaa caagcacccc cgaacggctt accccaacct tttccacaca
     2041 cccgatcacc aaaacaggga ataaaaacaa aaccctgtct tacaggtgga tggcctcact
     2101 t
//
