LOCUS       NZ_CP085035             2667 bp    DNA     circular CON 08-AUG-2023
DEFINITION  Klebsiella pneumoniae strain Kp_2016-1200 plasmid pKp1200_2,
            complete sequence.
ACCESSION   NZ_CP085035
VERSION     NZ_CP085035.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22106519
            Assembly: GCF_030644605.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Haakonsholm,F., Hetland,M.A.K., Loehr,I.H., Lunestad,B.T. and
            Marathe,N.P.
  TITLE     Colocalization of clinically relevant antibiotic and heavy metal
            resistance genes on plasmids in Klebsiella pneumoniae from marine
            bivalves
  JOURNAL   Microbiologyopen 12 (4) (2023)
  REMARK    DOI: 10.1002/mbo3.1368
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Hakonsholm,F.
  TITLE     Direct Submission
  JOURNAL   Submitted (14-OCT-2021) FRES, IMR, P.O. Box 1870 Nordnes, Bergen
            5089, Norway
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. 0.4.8
            Assembly Name                     :: Kp-ST25-2016-1200
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 342.0x
            Sequencing Technology             :: Oxford Nanopore GridION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/05/2023 07:47:53
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,337
            CDSs (total)                      :: 5,215
            Genes (coding)                    :: 5,106
            CDSs (with protein)               :: 5,106
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 53 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 18 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP085035.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="Kp_2016-1200"
                     /isolation_source="Bivalve mollusc"
                     /db_xref="taxon:573"
                     /plasmid="pKp1200_2"
                     /geo_loc_name="Norway"
                     /collection_date="2016"
                     /collected_by="Fredrik Haakonsholm"
     gene            complement(join(2581..2667,1..309))
                     /locus_tag="LGQ91_RS26675"
                     /old_locus_tag="LGQ91_26690"
     CDS             complement(join(2581..2667,1..309))
                     /locus_tag="LGQ91_RS26675"
                     /old_locus_tag="LGQ91_26690"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071067467.1"
                     /translation="MAFVSQVDKSRAASSRIGDSSIVVTQSYKASTKAQELSIRLTSLV
                     LSKGGMKIGDHVDVLYDKDSDRWMLRKMDGGLKITGKDDAPTGLIRYTLKDTHARFTEE
                     KEHLPIRKSSIEDSLEIEDGQIIFKLD"
                     /name="hypothetical protein"
     gene            544..819
                     /locus_tag="LGQ91_RS26680"
                     /old_locus_tag="LGQ91_26695"
     CDS             544..819
                     /locus_tag="LGQ91_RS26680"
                     /old_locus_tag="LGQ91_26695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="WP_224935441.1"
                     /translation="MQFRIESETKKMAKQALEKKGISLSDALRAFLDKLAATEKVMTKE
                     ETWLKEQIEETFSRVDKGEIRYYSEDEADERMNSFISKIENKHETA"
                     /name="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
     gene            complement(2306..2569)
                     /locus_tag="LGQ91_RS26685"
                     /old_locus_tag="LGQ91_26700"
     CDS             complement(2306..2569)
                     /locus_tag="LGQ91_RS26685"
                     /old_locus_tag="LGQ91_26700"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_004099073.1"
                     /translation="MTSKVLWADKALSDRETIFESNDKVSGLSYAKSEDAKIKRAVSTI
                     KRNNKVGRNDLDVRGYVYVLPKRYKILYRLTDEHIFIERVFY"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
ORIGIN
        1 ttcagtaaat ctcgcatgcg tatccttcag ggtatatcta atcaaaccgg tcggggcatc
       61 atccttacca gtaatcttta accctccatc catctttctt aacatccagc gatcactatc
      121 tttgtcatag agaacatcaa cgtgatcgcc aattttcatc ccacctttag acaacactaa
      181 tgatgtcaac ctaatggata gctcttgagc cttagtcgac gctttgtatg actgcgttac
      241 cactatagac gaatcaccaa tccgactcga agcagctcta gacttatcaa cctgactaac
      301 gaacgccata aaacctcctg gtagtttata ttcatgttta taatacgatc ggataaaaca
      361 cttagcaaca cagatctatg tttttagctg atctctaaac tctctcaaaa tccgaagaaa
      421 aaagttactt ttttccaagc ataaatctaa gaaaggaagc attgactgta gggatcccag
      481 agtctattgt gagcacaaac gtactcacaa tagactctgg gatctatatc tatggaaaca
      541 agggtgcaat tcagaattga atctgaaaca aagaaaatgg caaagcaagc gcttgaaaag
      601 aaagggattt cacttagcga cgcgctaagg gcgtttctcg ataagttagc agcgacggag
      661 aaggttatga caaaagaaga gacatggctg aaagagcaga tagaagagac attctcaagg
      721 gtagataaag gagagatccg ctattactca gaagatgaag ctgatgaacg catgaactcc
      781 ttcatctcaa aaatcgagaa caaacacgaa actgcataaa aaaagccgcc aatggcggct
      841 ttttttatgc ccttatttac cataatccct taattgtacg caccgcccaa gcggtctcag
      901 ggcgtttccg acgggaaacc ggtaaaacag gcaacaaaca acccgaaacc ccgccagaaa
      961 gcgcaggctc tgtttttacg gcatgcatga gtatgcgagc aagtgcataa cccggaaatt
     1021 cacaccattc agccagggta agtcctgttt ccggcgttct tctcctccga aaacggtgaa
     1081 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgaagccgga aaaccggttt
     1141 tcagcacgtt caggaatcgg gttcgtcgac gacctgcaat cacgttatgg caagtcggcg
     1201 gacgactcac cacacgattc accggcgatt cgactcgtga cttgactcta gagtcgccaa
     1261 ggtcacaaaa acaccaaaat gacgattcga ctctagagtc aagtcgtcat aagttactga
     1321 tagtcttaag tgagtcgtcg atgactctat gacaagtcgt cggcaagtcg tacacgaatc
     1381 gccgacgact tggattcacc ttacagaaag taatgctgcc taatttaata actcacaact
     1441 aactgatatt aatgagttat cttcaaaatt ttaattttgc gtggggtgtg ggtattttta
     1501 gtggtgagga acattcagag agttacctgt tttcgggaag gtttaagaac atacccgaaa
     1561 ggaaaatgag aaaccggagc gtcaggcagc tccgggtaac acggtgactc ttccgcttcc
     1621 tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggtggg gcttttacac
     1681 aaaggagtcc atgaaagtga gaaaaaaaag atgagcactg gccgtttttc cataggcttc
     1741 gcccccctga cgagcatgac aagatcttac gctcaaatca gtggtggcga aacccgacag
     1801 gactataaag atcccaggcg tttccccctg gtggctcccc cgtgcgctct cctgttcctg
     1861 ccgttcggtt taccggtgtc actccgctgt tatggccgcg tttatctcat tccacgcctg
     1921 acactcagtt ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt
     1981 cagtccgacc cccacgacaa acaagcaact cgactcttga atccaatctt gtttgactca
     2041 aattatgaca atggtggaag cccgtaataa ctgatttgcg gagcgcagaa agaaggtaag
     2101 caaggatatc ttgaagtgag cagtgaacta cggctacact gaaagaacag ttttggtgac
     2161 tgcgctccgc taaagccagt taccaccagt ccaatgagtt ggtagctcaa aggactacga
     2221 agaaaccacc gttggtagcg gtggttttct tcatcccaag attaatagct aaagagacgc
     2281 gcagtcaaga aaggtcggca aaccatcagt agaaaacgcg ctcaataaaa atatgctcat
     2341 ccgtcagtcg gtacaaaatc ttgtatcttt taggtagaac atagacatag cctcgcacat
     2401 ctaggtcatt tctgcctact ttgttgtttc gttttatcgt ggaaacagca cgtttaattt
     2461 tggcatcttc tgattttgca tacgacaaac cagagacctt atcgttagac tcaaagatag
     2521 tctctcgatc agataatgcc ttatctgccc ataaaacttt agaagtcatc aaacctcccg
     2581 ctaatcaagc ttaaaaataa tctgcccatc ctcaatctcc agagaatcct caatagagga
     2641 tttacgaata gggaggtgct ctttctc
//
