LOCUS       NZ_CP084989             3989 bp    DNA     circular CON 11-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP18-2110-2 plasmid pKP18-2110-2-3,
            complete sequence.
ACCESSION   NZ_CP084989
VERSION     NZ_CP084989.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21156845
            Assembly: GCF_020535145.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Qin,S.
  TITLE     Complete sequence of Klebsiella pneumoniae strain KP18-2110-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Qin,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-2021) Institute of Drug Discovery & Development,
            Zhengzhou University, 100 Science Avenue, Zhengzhou City, Zhengzhou,
            Henan 450001, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: unicycler v. v.0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 12.0x
            Sequencing Technology             :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/11/2024 06:23:11
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,768
            CDSs (total)                      :: 5,647
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 121
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 10
            Pseudo Genes (total)              :: 100
            CDSs (without protein)            :: 100
            Pseudo Genes (ambiguous residues) :: 0 of 100
            Pseudo Genes (frameshifted)       :: 35 of 100
            Pseudo Genes (incomplete)         :: 64 of 100
            Pseudo Genes (internal stop)      :: 13 of 100
            Pseudo Genes (multiple problems)  :: 11 of 100
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084989.1.
            Bacteria and source DNA available from Patient.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP18-2110-2"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKP18-2110-2-3"
                     /geo_loc_name="China"
                     /lat_lon="34.73 N 113.70 E"
                     /collection_date="2018-11-30"
                     /collected_by="Zhengzhou University"
     gene            join(3338..3989,1..323)
                     /locus_tag="LH404_RS28805"
     CDS             join(3338..3989,1..323)
                     /locus_tag="LH404_RS28805"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_114269875.1"
                     /translation="MAFFNLLFVASLICFGGVMGSYSRGCRDSQNKFSKDKDENNKRAS
                     VYRFGIASAFICVPFISSFLHVDYSSIIFPVADGGGTKFIEQILLLISVSGISAYLGYA
                     LLDGLANKVLKEQVDGIDKKQQDLEAEQDEFKDELDRSKELIEQLEMDKKTTKFELGYF
                     KAISAVDKAESMMSIPDEALSVKKKLTEALDAVTESLSLVKREDVAKDDYDKLLVLKAY
                     ILKRLDRIDDALSITDELLMSNEDNPILIYNKACYQYILRRCQADNSDIKDMIRRALTI
                     KVTDPEFIRRQEKIRTKVIGNKDNDLEGLFTDAELEELKVAIK"
                     /name="hypothetical protein"
     gene            complement(550..662)
                     /locus_tag="LH404_RS28810"
                     /pseudo=""
     CDS             complement(550..662)
                     /locus_tag="LH404_RS28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            1070..1261
                     /locus_tag="LH404_RS28815"
     CDS             1070..1261
                     /locus_tag="LH404_RS28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_040198051.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLDKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            1261..1683
                     /locus_tag="LH404_RS28820"
     CDS             1261..1683
                     /locus_tag="LH404_RS28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_040214596.1"
                     /translation="MAIVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKAIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            2487..2759
                     /locus_tag="LH404_RS28825"
     CDS             2487..2759
                     /locus_tag="LH404_RS28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004897311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_019405140.1"
                     /translation="MATDAQVKEINALIKKYPDSCSICHEVYDEDDVTYTVFGYDRKGK
                     IQVTTGCCAGMLTEPVLLGVCGCFDPEERDEIMQNHPMAKQFFTE"
                     /name="hypothetical protein"
     Typing          complement(551..1022)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.777"
                     /coverage="92.0"
                     /evalue="7.8799999999999974e-146"
ORIGIN
        1 tgcttaaagc ttatattttg aaaagacttg atcgtattga tgatgctctg tcaattacgg
       61 atgagttact tatgagtaat gaggataacc ctatacttat ttacaataag gcttgctatc
      121 aatatatttt gcgtcgttgc caagctgata actccgatat taaggatatg attcgcaggg
      181 ctctgactat taaagttaca gatcctgaat tcattcgcag gcaagaaaaa ataagaacca
      241 aggtcatagg aaataaagat aatgatcttg aagggttgtt tactgacgct gagctggagg
      301 aattaaaggt ggctatcaaa taggcagtgg ctagctctat gaattccaaa aagatccttg
      361 acgatccttt gaggtgcagc gtactctacc cctcaaacga aaaaaccacc cggcaaggtg
      421 gtttttcgaa ggttcagtaa ccggcaagtt tctgaaccga ggtaactggc ttagaggagc
      481 gcagtcacca aaacagttcg tttagtttag ccttaactgg cgcataactt caagactaac
      541 tcctctaaat caattaccag tggctgctgc caatggcgat aagtcgtgtc tttccgggtt
      601 ggactcaaga gatagttact ggaacgggcg cagcagtcgg actgaacggg gggttcgtgc
      661 atacagtcca gcttggagcg aactgccttc ccggagccga gtatcaggca tggaatgaga
      721 aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa aggcaggaac aggagagcgc
      781 acgagggagc caccaggggg aaacgcctgg tatctttaag tcctgtcggg tttcgccacc
      841 actgatttga gcgtccgttt ccgcgatgct tgtcaggggg gcggagccta tggaaaaaca
      901 gccgttcggc ggcctttgct tttctgcctc atatcttggt tgcctacgtt tttatgtaca
      961 cgctcctgcc gctcgccgca gccgaacggc tggagcgtag cgactgagtg agcgaggaag
     1021 cggaaaagag actagtttga cattgagcac tgacgctctg aggcttctta tgacaaaaca
     1081 acagaaaaca gcgctgaaca tggctaaatt catccagaat cagtctctgc tcctgctgga
     1141 taagcttaac gagcttgatc tcgatgtcga ggccgacctt tgtgagaaac tccatgatga
     1201 tgcggagcat cttttccgta cactgtcctc gagactggat gaattacagg atggtaactg
     1261 atggccatag tgtctattag tgaagcggca cggcttgtta aggttagtcg ccccacaatt
     1321 tacaagatga ttaacagtgg gaagttaagt tacacctctg tggtaaagca cgggaaagct
     1381 attaaagtta ttgatacttc agagctaatt agagtgtttg gttctcttga tggtgtaatc
     1441 gatactgtaa aatatgatgt aaaaagtgac gcagaatcta caggtattaa cagcgttggt
     1501 ttacatgatt tacaacatag aatcgcttta cttgaagctg aaaacgatgg tcttaaaggg
     1561 gctgttaagg ctcgagatga gcatattgat tcccttcgtc aggctatgca gcttctggag
     1621 cacaagcacg aaccttcttc accaccccat tccccgtggt ggaaattctg gaaaaaatcc
     1681 tgatcccgcc atgcttccat tttgctctcc ggccattccg gggagttcct gaaccttccc
     1741 aaaaatcgtc agatccctga acgctcctca ccactaaaaa tacccacaca ctacgcaaaa
     1801 ttaaaatttt gcagatagac ctttattttc aatcagttat ctattttaaa ttacgcaatt
     1861 ttattttttg taaggtgaat ccgagtcgtg gacgactcta tggcgaatcg ccgatgactt
     1921 gcccacgatt catatgtgaa tcacgccata taatcagtac gttatgatga cttgactcta
     1981 gagtcgactt gccgggttgg tggctttttg accatggcga ctctagagtc aagtcacgag
     2041 tcgaatcgcc ggtgaatcgt gtggtgagtc gtccggcgac ttgccataat gtgattgctg
     2101 gtctcgacaa acccgattcc tgaacgtgct gaaaaccggt tttccggctg cgccggcaac
     2161 gcttcacggc cacgcaataa attgcgcacc cgttcaccgt tttcagtctg gtttggtgcg
     2221 ggttttgagt tgcctggcct gaattcaggc tgaaatcccc agacagtcag tcaagtgcat
     2281 aacgattcat gcccgtaaaa cgccctggag cgattttgag gcgttttcag ggggtaagac
     2341 gtaggattta atgggttacg ccccagaatc gttctgaggc cgttttagcg gtgcgtgtaa
     2401 tgggacgtta tggtaaatca acggcggatg cctgagtctg tgttagagtc agcggattgt
     2461 gataatcagt gactcaggga gatgttttgg ctactgacgc tcaggtaaaa gaaattaatg
     2521 cactcatcaa aaaatacccg gactcatgct ccatctgtca tgaagtctat gatgaggatg
     2581 atgtgaccta caccgttttt gggtatgacc ggaaaggtaa aatacaggtc accacgggat
     2641 gctgtgccgg aatgctgaca gagccggttc tgctgggtgt ctgtgggtgc tttgacccgg
     2701 aagaacgtga cgagatcatg cagaatcacc ccatggcaaa gcagtttttc acagagtgat
     2761 tccgtatctg ttgagtctga agcccgcctt gtgcgggctt tttcatatct gcgtgaaatg
     2821 aggcgttatg gtaaatgcac gcattcattc ttttgaaaaa tccagaatga cgaatacatg
     2881 gggcatgttt atatgactat tgagaggaag gatgttattg taaaaaaatc ctttaaattc
     2941 aatcatttaa ttatggtagc caaaggggcg gggcttcgct tattcctaag gaataagcga
     3001 agccccgccc ctggctacca gtgcattata cttttttttt cacccaatgt caatacatgg
     3061 gttgatttta tcattttaag catttttgtt gttgtgagtg ctgggtttat gccctatatt
     3121 cagcgctaag caagcatggg cttgcaaagg aacaattaat gagaggtgct ctatgaaaaa
     3181 aatcaaactt cacgatgagc gtccagactt gtaagtgtca agtcagcaga aaaaggcagc
     3241 caaggctgcc ttttttgtta ttggttagta tatgattgag cttatcttaa cttacctgaa
     3301 caaagtttta ttgtttgctt tgcgcaagga ttctctcatg gcttttttta acctattgtt
     3361 tgttgcatct ctaatttgtt ttggtggtgt tatggggagc tattccagag gttgccgaga
     3421 ctcccaaaat aaattttcaa aagataagga tgagaataat aaaagagcta gtgtttatag
     3481 atttggtata gcatccgcat ttatttgtgt tcctttcata tcatcatttc ttcatgttga
     3541 ttactcaagt ataatatttc ctgtggctga tggtggtgga actaagttca tagaacaaat
     3601 attgcttctt atttctgttt cggggatctc tgcgtatctt gggtacgcat tactggatgg
     3661 tcttgccaac aaagtattaa aagagcaagt agacggtata gataaaaagc aacaagattt
     3721 ggaggctgaa caagatgagt ttaaagatga gcttgatagg agcaaggagc tgatagaaca
     3781 gcttgaaatg gataagaaaa caacaaaatt cgagctagga tactttaaag ccatttcagc
     3841 tgtggataaa gcagaatcaa tgatgtccat tccggatgag gctctttctg ttaagaaaaa
     3901 actaactgag gcattagatg ctgtaacgga atcattgtct cttgttaaaa gagaggatgt
     3961 agctaaggat gactacgaca agctcttag
//
