LOCUS       NZ_CP084907              614 bp    DNA     linear   CON 17-NOV-2023
DEFINITION  Escherichia coli strain EC20 plasmid pEC22-7.
ACCESSION   NZ_CP084907
VERSION     NZ_CP084907.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21906625
            Assembly: GCF_020535205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Huang,J.
  TITLE     Whole genome sequence of EC22, an NDM-5-, CTX-M-15-, and
            blaOXA-1-co-producing Escherichia coli ST361 clinical isolate from
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 614)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/17/2023 09:33:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 86 of 277
            Pseudo Genes (incomplete)         :: 178 of 277
            Pseudo Genes (internal stop)      :: 56 of 277
            Pseudo Genes (multiple problems)  :: 40 of 277
            Pseudo Genes (short protein)      :: 4 of 277
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084907.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-7"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-10-18"
                     /genotype="ST11"
     gene            35..274
                     /locus_tag="LHL15_RS25845"
     CDS             35..274
                     /locus_tag="LHL15_RS25845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001549892.1"
                     /translation="MPRHYEIDSAWRASIKREPNGRQTVTTEAFVSQLALINFHWSCRQ
                     ANQWIETYVTVFKDISTQEGENRTFMLFNPNGGR"
                     /name="hypothetical protein"
     gene            277..>614
                     /locus_tag="LHL15_RS25850"
     CDS             277..>614
                     /locus_tag="LHL15_RS25850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004199403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="WP_032428144.1"
                     /translation="MGFPSPATDYQENRLSLDQHCNTGAPGVFFFRSDTYSFREGIKPG
                     ALLIVDFGGTPVDGSLVLCVLEQEFRIMRLRLHPKRCLQELDKLDNFRAIPDDDEDGLE
                     VRGVITHIV"
                     /name="S24 family peptidase"
ORIGIN
        1 atatgcatac agctatattg atcggaggtt tttcatgcct agacactatg aaattgactc
       61 agcctggcgt gcgagcatta agcgtgagcc gaacggtcgt cagacagtta cgacagaagc
      121 ctttgtcagc cagcttgcgc tgattaattt tcactggagt tgtcgccaag caaatcagtg
      181 gattgaaacc tacgtgaccg tttttaaaga catttccact caggaaggag agaaccggac
      241 attcatgctt tttaacccca acggaggccg ttaaaaatgg gttttccatc gccagcaacc
      301 gactaccagg aaaacaggct gtcactcgat cagcactgta atacaggagc gccgggagta
      361 ttcttttttc gaagtgatac ctactctttc cgggaaggga taaagcctgg tgcgctgctc
      421 atagtagatt ttgggggcac gcccgttgac gggtctttgg tgctctgtgt cctggaacaa
      481 gaattcagga tcatgcgatt gcgactacac cccaagcggt gtctccagga actcgataag
      541 ctggataact tcagggctat acctgatgac gatgaggacg ggttggaggt caggggggtc
      601 attacgcaca ttgt
//
