LOCUS       NZ_CP084906              670 bp    DNA     linear   CON 17-NOV-2023
DEFINITION  Escherichia coli strain EC20 plasmid pEC22-6.
ACCESSION   NZ_CP084906
VERSION     NZ_CP084906.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21906625
            Assembly: GCF_020535205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Huang,J.
  TITLE     Whole genome sequence of EC22, an NDM-5-, CTX-M-15-, and
            blaOXA-1-co-producing Escherichia coli ST361 clinical isolate from
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 670)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/17/2023 09:33:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 86 of 277
            Pseudo Genes (incomplete)         :: 178 of 277
            Pseudo Genes (internal stop)      :: 56 of 277
            Pseudo Genes (multiple problems)  :: 40 of 277
            Pseudo Genes (short protein)      :: 4 of 277
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084906.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-6"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-10-18"
                     /genotype="ST11"
     gene            63..>670
                     /locus_tag="LHL15_RS25840"
     CDS             63..>670
                     /locus_tag="LHL15_RS25840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="WP_001067849.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDHS
                     TIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTVDF
                     YLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDVEHR
                     QIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGI"
                     /name="IS6-like element IS26 family transposase"
ORIGIN
        1 gcactgttgc aaatagtcgg tggtgataaa cttatcatcc ccttttgctg atggagctgc
       61 acatgaaccc attcaaaggc cggcattttc agcgtgacat cattctgtgg gccgtacgct
      121 ggtactgcaa atacggcatc agttaccgtg agctgcagga gatgctggct gaacgcggag
      181 tgaatgtcga tcactccacg atttaccgct gggttcagcg ttatgcgcct gaaatggaaa
      241 aacggctgcg ctggtactgg cgtaaccctt ccgatctttg cccgtggcac atggatgaaa
      301 cctacgtgaa ggtcaatggc cgctgggcgt atctgtaccg ggccgtcgac agccggggcc
      361 gcactgtcga tttttatctc tcctcccgtc gtaacagcaa agctgcatac cggtttctgg
      421 gtaaaatcct caacaacgtg aagaagtggc agatcccgcg attcatcaac acggataaag
      481 cgcccgccta tggtcgcgcg cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg
      541 ttgaacaccg acagattaag taccggaaca acgtgattga atgcgatcat ggcaaactga
      601 aacggataat cggcgccacg ctgggattta aatccatgaa gacggcttac gccaccatca
      661 aaggtattga
//
