LOCUS       NZ_CP084905             3174 bp    DNA     circular CON 17-NOV-2023
DEFINITION  Escherichia coli strain EC20 plasmid pEC22-5, complete sequence.
ACCESSION   NZ_CP084905
VERSION     NZ_CP084905.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21906625
            Assembly: GCF_020535205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Huang,J.
  TITLE     Whole genome sequence of EC22, an NDM-5-, CTX-M-15-, and
            blaOXA-1-co-producing Escherichia coli ST361 clinical isolate from
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/17/2023 09:33:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 86 of 277
            Pseudo Genes (incomplete)         :: 178 of 277
            Pseudo Genes (internal stop)      :: 56 of 277
            Pseudo Genes (multiple problems)  :: 40 of 277
            Pseudo Genes (short protein)      :: 4 of 277
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084905.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-5"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-10-18"
                     /genotype="ST11"
     gene            complement(210..404)
                     /locus_tag="LHL15_RS25815"
     CDS             complement(210..404)
                     /locus_tag="LHL15_RS25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_001485273.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHELA
                     EELQNSIQTRFEAENLTGI"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            688..1011
                     /gene="mobA"
                     /locus_tag="LHL15_RS25820"
     CDS             688..1011
                     /gene="mobA"
                     /locus_tag="LHL15_RS25820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="WP_052584083.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQVIRKRAAECGKTVSGFLRA
                     AALGKKVNSLTDDRVLKEVMRLGALQKKLFIDGKRVGDREYAEVLIAITEYHRALLSRL
                     MAD"
                     /name="mobA"
     gene            complement(1400..1828)
                     /locus_tag="LHL15_RS25825"
     CDS             complement(1400..1828)
                     /locus_tag="LHL15_RS25825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222531.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_000222531.1"
                     /translation="MTYSVKNKWKAGGLSLGWTAVPTVLFFIQNEKKLTSVAFNTLLNL
                     IVHWWSLQEWPHPSMESLAIRMGVSVRTVQRAINDLEKANLLDKKPTSKSDRRYGGRNI
                     YDLTKLVDYLDTMGPSVAEQVKKPRHKKPVYTVRKTTA"
                     /name="helix-turn-helix domain-containing protein"
     gene            1986..2156
                     /locus_tag="LHL15_RS25830"
     CDS             1986..2156
                     /locus_tag="LHL15_RS25830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001531327.1"
                     /translation="MKTEQRHDLVKDIEVFGVSLSLLISRANEKSVTMPSGLSREQRRA
                     WAAEQARKIHN"
                     /name="hypothetical protein"
     gene            2120..2548
                     /locus_tag="LHL15_RS25835"
     CDS             2120..2548
                     /locus_tag="LHL15_RS25835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000534558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000534558.1"
                     /translation="MGSGAGAQNPQLNIVSFSETFNLYYTSRYSAKTLDKIDDFIEHFE
                     KYNLFGDPLKGYPAWTGKVSPSWKVPDHYENKEAIEKYARANKLWHAHLGDPVFKDTFH
                     GKYKVSDWVIHFQRLTPNHIKLLELGYHDPMKLPQNFE"
                     /name="hypothetical protein"
     Typing          440..679
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="7.6e-123"
     Typing          complement(5..175)
                     /name="rep_cluster_2350"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.767"
                     /coverage="91.0"
                     /evalue="4.34e-69"
     Typing          complement(2769..3173)
                     /name="rep_cluster_2370"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="95.567"
                     /coverage="94.0"
                     /evalue="0.0"
     Typing          689..1008
                     /name="MOBF"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="98.131"
                     /coverage="100.0"
                     /evalue="2.92e-61"
ORIGIN
        1 tggcttcggc tttttgctca catgttcttt ccggcgttat cccctgattc tgtggataac
       61 cgtattaccg cctttgagtg agctgacacc gctcgccgca gtcgaacgac cgagcgtagc
      121 gagtcagtga gcgaggaagc ggaagagcgc cttatgtgac attttctcct tacgctcctc
      181 tatgccgttc tgcatcctgt ccggatgcgt tatatcccgg taagattttc cgcttcaaag
      241 cgtgtctgta tgctgttctg aagttcttcc gcgagttcgt gcagcttctc acacatggcg
      301 gcctgttcgt cagcatcaag tgcgtccagt ttttcgagca gcgtcaggct ctggcttttt
      361 atgaatcctg ccatgttgag tacggtttgc tgctgcttgt tcatctttct gttttctccg
      421 ttctttctgt catctgcgtt gtgtgattat atcgcgtacc acttttcgac tgttttaccg
      481 tcggtattct gctgtctgac tctttgacgg gcatttctgt cagacaacac tgtcactgcc
      541 aaaaaactgc cgtgcctttg tcggtaattc gaacttgctg acaggacagg atgtgtaatt
      601 gttataccgc gcatacatgc acgctattac aattaccctg gtcagggctt tgccccgaca
      661 cccatgtcag atacggagcc atgttttatg acaaaacgaa gtggaagtaa tacgcgcagg
      721 cgggctatca gtcgccctgt tcgtctgacg gcagaagaag accaggtaat cagaaaaagg
      781 gctgctgaat gcggcaagac cgtttccggt tttttacggg cggcagctct cggtaagaaa
      841 gttaactcac tgactgatga tcgggtactg aaagaagtta tgagactggg ggcgttacag
      901 aaaaaactct ttatcgacgg caagcgtgtc ggggacaggg agtatgcgga ggtgctgatc
      961 gctattacgg agtatcaccg tgccctgtta tccaggctta tggcagatta gcttcccgga
     1021 gagaaaactg tcgaaaactg acggtatgaa caccgtaagc tcccaaagtg atcaccattc
     1081 gctttcatgc atagctatgc agcgagctga aaacgatcct gacgcatcct tcctgttttt
     1141 ccggggtaaa acatctcttt ttgcggtgtc tgcgtcagaa tcgcgttcag cgcgtttcag
     1201 tggtgcgtac aattaaggga ttatggtaaa tatatgagct atgcgataac tttaactgtg
     1261 aagcgatgaa cccattacag gcaaagccaa ttactcctga cagtggttta gccagaagca
     1321 gggctaccaa gaccaatgca ataagtaata tatcgttttg ctatcgtgca tcgctcgcgc
     1381 tcagttccat tgtgcttttt taagctgtcg tttttcttac ggtatatacc ggttttttat
     1441 ggcgtggttt cttaacttgt tcagctactg atggacccat tgtatctagg tagtcaacta
     1501 gctttgttag atcataaata ttgcgaccac catatcggcg atcactcttc gatgttggtt
     1561 tcttgtccaa gagattagct ttttcaagat cattgatagc tctctgaaca gtccgtacag
     1621 aaacccccat acgtatggct agactttcca ttgacggatg cggccactct tgcaaactcc
     1681 accagtgaac gatcaggtta agtagtgtgt taaaggccac tgaagttagc tttttctcgt
     1741 tttgtataaa aaacaatacg gtaggcactg ctgtccagcc aagagacaaa ccgccagctt
     1801 tccatttatt cttaacggag taagtcattg attttcctaa gccccaaaat atttaaagta
     1861 tatattatat ggtatattca tatgaatagg gtgacactag cgccattatt gtgcaaccaa
     1921 aaaagactac tctgaaaacg aggaaaagat tttttcctgc ctgaattaga tacggagtta
     1981 gcgatatgaa aaccgaacaa cgtcatgatc ttgttaaaga tattgaggtt tttggcgtat
     2041 ccttgtctct gttgatttcc agagcgaatg agaagtctgt tacaatgcca tctggtctaa
     2101 gtcgggagca gagaagagca tgggcagcgg agcaggcgcg caaaatccac aattgaatat
     2161 tgtctcattc tctgagacct tcaaccttta ttacacatcc agatattctg caaaaacact
     2221 cgataaaatc gatgatttca ttgagcattt tgaaaaatac aatctctttg gcgatccttt
     2281 aaaaggatat ccagcttgga ctggcaaagt atcgccatcg tggaaagtgc ctgatcatta
     2341 cgaaaacaaa gaagctattg agaagtatgc tagagctaac aaattatggc atgctcattt
     2401 aggcgatccg gtttttaaag atacgtttca tgggaaatac aaggtttctg actgggttat
     2461 tcatttccag cggctgacac cgaaccatat aaagctttta gagcttggat accatgaccc
     2521 aatgaagcta cctcaaaact ttgaatgatc gagcggcagg ctaaatgaaa tcttgagatt
     2581 cattcagtct cgtcgtaatc tcactattgt aaaaacgaaa aaaccaccct ggcaggtggt
     2641 ttttcgaagg ttagttaatc ctggcagatt ctctaaccgt ggtaacagtc ttgtgcgaga
     2701 catgtcacca aatactgtcc tttcagtgta gcctcagtta ggccgccact tcaagaactc
     2761 tcgttacatc tctcgcacat cctgcttacc agtggccgtt gccagtggcg ttaagtcgtg
     2821 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     2881 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac cgagatacca
     2941 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     3001 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     3061 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcggt ttttgtgatg
     3121 ctcgtcaggg gggcggagcc tatggaaaaa cgccaacggc gcggcctttg tccc
//
