LOCUS       NZ_CP084903            10227 bp    DNA     circular CON 17-NOV-2023
DEFINITION  Escherichia coli strain EC20 plasmid pEC22-3, complete sequence.
ACCESSION   NZ_CP084903
VERSION     NZ_CP084903.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21906625
            Assembly: GCF_020535205.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10227)
  AUTHORS   Huang,J.
  TITLE     Whole genome sequence of EC22, an NDM-5-, CTX-M-15-, and
            blaOXA-1-co-producing Escherichia coli ST361 clinical isolate from
            China
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10227)
  AUTHORS   Huang,J.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-SEP-2021) Department of Clinical Laboratory, Jinhua
            Central Hospital, Mingyue street 351, Jinhua, Zhejiang 321000, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: Oxford Nanopore PromethION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 11/17/2023 09:33:46
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,202
            CDSs (total)                      :: 5,084
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 118
            rRNAs                             :: 8, 7, 7 (5S, 16S, 23S)
            complete rRNAs                    :: 8, 7, 7 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 277
            CDSs (without protein)            :: 277
            Pseudo Genes (ambiguous residues) :: 0 of 277
            Pseudo Genes (frameshifted)       :: 86 of 277
            Pseudo Genes (incomplete)         :: 178 of 277
            Pseudo Genes (internal stop)      :: 56 of 277
            Pseudo Genes (multiple problems)  :: 40 of 277
            Pseudo Genes (short protein)      :: 4 of 277
            CRISPR Arrays                     :: 3
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084903.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /strain="EC20"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /plasmid="pEC22-3"
                     /geo_loc_name="China: Jinhua"
                     /collection_date="2019-10-18"
                     /genotype="ST11"
     gene            68..1288
                     /locus_tag="LHL15_RS25695"
     CDS             68..1288
                     /locus_tag="LHL15_RS25695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISKox3 family transposase"
                     /protein_id="WP_000343760.1"
                     /translation="MDEKSLYAHILNLSDPWQVKSLSLDENAGSVTVTIEIAENTRLAC
                     PTCGKSCSVHDHRHRKWRHLDTCQFTTIVEADVPRIMCPEHGCLTLPVPWAGPGSRYTL
                     LFESFVLSWLKISTVDAVRKQLKLSWNAVDGIMTRAVKRGLARIKKPLSARHMNVDEVA
                     FKKGHRYITVISDRDGRALALTDDRGTESLAGYLRTLTDGQLLAIKTLSMDMNAGYIRA
                     ARIHLPSAVEKIAFDRFHVAKQLGEVVDKTRQNEHPHLPVESRHQAKGTRFLWQYSDKW
                     MTESRQEKLMWLRAQMKLTSQCWALKELAKDIWNRPWSEERRSDWQRWLALAANSDVPM
                     MKNAAKTIGKRLYGILNAMRHSVSNGNAEALNSKIRLLRIKARGYRNRERFKLGVMFHY
                     GKLNMAF"
                     /name="ISL3-like element ISKox3 family transposase"
     gene            complement(1377..2039)
                     /locus_tag="LHL15_RS25700"
     CDS             complement(1377..2039)
                     /locus_tag="LHL15_RS25700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549893.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001549893.1"
                     /translation="MTQTVPKGFVRAYLRASTTEQDATRALDTINTFAIERGLSICNYY
                     IENESGSKLERPELFRLLKDCQQNDILLVEDVDRLSRLVGEDWNTLKKMIRQKDIRVVA
                     VNVPTTWLASGHNDFDSRMFSAINDMLLDMLAAVARRDYEQRRERQQQGIARAKREGKY
                     KGRQVNQSRYDAINRLIASGSSWSQIQKVLGCSRATISKAVKQQTVNKMNHEPEGRE"
                     /name="recombinase family protein"
     gene            2420..3082
                     /locus_tag="LHL15_RS25705"
     CDS             2420..3082
                     /locus_tag="LHL15_RS25705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="WP_000516402.1"
                     /translation="MGKILLVVSDKGGVGKSTYVANTGSMLVNKGKSVIILKTDKNHDL
                     LSWNEKRTDNGLLTIPVHAAYGNVSNEIKRLSKLCEVLIVDCPGHDSQEFRSALTVSDI
                     VVTLVKPSSDFESETLTSVTEKIRTAQKANAALEPWVLFTRINSAKPRHRKAAIDLDKL
                     LRSDNIWIQPLKTRISELDVYESACNEGAGVHDVSRASSLSTAKAQIELVAQEIGIL"
                     /name="division plane positioning ATPase MipZ"
     gene            3182..3466
                     /locus_tag="LHL15_RS25710"
     CDS             3182..3466
                     /locus_tag="LHL15_RS25710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001185481.1"
                     /translation="MALKLNRPNIDESQQPATNAETARFISGATRAPVAGKPKLMNFRL
                     PAYFEEILEAEAQRTGQSKTTVLKAALAAFDNLDENLKNHWLLESAKLG"
                     /name="hypothetical protein"
     gene            3672..3983
                     /locus_tag="LHL15_RS25715"
     CDS             3672..3983
                     /locus_tag="LHL15_RS25715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000469598.1"
                     /translation="MFIFRYIKTLINIYLLYIQPILGMFLIGIGLWGFYAPDEGTCTNL
                     NIELCAVSAIDVLKPAIFVFLGIITVAGWYFTELQPKLRGEGKYMKVKTGVKPEDFVK"
                     /name="hypothetical protein"
     gene            4019..4303
                     /locus_tag="LHL15_RS25720"
     CDS             4019..4303
                     /locus_tag="LHL15_RS25720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001043731.1"
                     /translation="MNKSWLEQQVQNEKLKKVQEEKKDGTIQKGLYRVRIKDKYRWDLW
                     ENDFRVVIAYSKDDAELKFTEMTGFNEHYYIESIEKIVLSDIEKILLEN"
                     /name="hypothetical protein"
     gene            complement(4324..4677)
                     /gene="ddp1"
                     /locus_tag="LHL15_RS25725"
     CDS             complement(4324..4677)
                     /gene="ddp1"
                     /locus_tag="LHL15_RS25725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA distortion polypeptide 3"
                     /protein_id="WP_000877224.1"
                     /translation="MKYQVKEFINEKYSKAVNILKDNLKEHYHIFYGLRLSEILFPASE
                     YGSEMFFQEFEAINSVILPLVIFDLIDRKPIMVIGFGEVCGVDSLVDSGIEVVSLDGLS
                     DLLLVEKLTPLFN"
                     /name="ddp1"
     gene            complement(4865..5308)
                     /locus_tag="LHL15_RS25730"
     CDS             complement(4865..5308)
                     /locus_tag="LHL15_RS25730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001074369.1"
                     /translation="MAKIYEFPQGAERSKLKKEIIRERKKRLRETNGNPVIRHAKWFWF
                     YLRLATAGALHLVSVISLAVLGAFSKAIFWIGGMLCVVTWFHLERQFWTPQNFTIPVIV
                     TLWGLSLFATPLMELLNKKMPWYRLLVPDAKHTSTEATNDDQP"
                     /name="hypothetical protein"
     gene            complement(5317..6330)
                     /locus_tag="LHL15_RS25735"
     CDS             complement(5317..6330)
                     /locus_tag="LHL15_RS25735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348704.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_001348704.1"
                     /translation="MRLLLSLDMKILRSSYLKVRGFLIKGCIVVLNYNLCGVMICLHRK
                     GYMRLIVKTVTGLTKVRHRNEVGVTLASLSLSAKRVLFLALCQIDTKEMLDDDILEVDA
                     DFFSKATSLDKYASYAALKEGAKVLSSTTLVLNRDDLKNLADELGILSSKNKIPDRLDL
                     NLTEFCAYYDHLATVRIKFTNTAKRYFSKLIGSENRYTTQVLKSVVLLNSVNSTNLYQV
                     IRKYYSQNSSKKSFDISVDDLKEEMGLYTIEEGEKKYKYPKYSFFVRDVINKSINEIIE
                     KTEINQLSFSVVGKKGRMAHMLRFEFSINEKSSSFSEDDMAFLEEFDKVVPPKKNK"
                     /name="replication initiation protein"
     gene            <6941..7093
                     /locus_tag="LHL15_RS25740"
                     /pseudo=""
     CDS             <6941..7093
                     /locus_tag="LHL15_RS25740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /name="DNA-binding protein"
     gene            complement(7271..7429)
                     /locus_tag="LHL15_RS25745"
     CDS             complement(7271..7429)
                     /locus_tag="LHL15_RS25745"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_152913118.1"
                     /translation="MRAITPLTPARPISDYQTMFSLSQLARELANGTPGQCETLARGAV
                     SARYEMR"
                     /name="hypothetical protein"
     gene            7683..8198
                     /locus_tag="LHL15_RS25750"
     CDS             7683..8198
                     /locus_tag="LHL15_RS25750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="WP_001025390.1"
                     /translation="MNIQEALNVFGLSGDLTEKDIKAAYKKAALKYHPDRNPLGAELMK
                     AVNAAFDFLMANIDKINQFQSTDENARYNYGEDLEKVLNTLSGLTGIVYEVIGNWVWIS
                     GETKEHKDILKEMGCKWASKKKQWFYRPEEHKSRWNRKEHSIEEIREMYGTAGKRKASG
                     WTRVEASA"
                     /name="DnaJ domain-containing protein"
     gene            8257..8493
                     /locus_tag="LHL15_RS25755"
     CDS             8257..8493
                     /locus_tag="LHL15_RS25755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006122499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000792381.1"
                     /translation="MKNKTITEAELINIFESYGAYICPDEIEVTAKECNENGSVLHRGL
                     NAEGWAHLFAKEEAYQQECEAQEAASDDGHFDE"
                     /name="hypothetical protein"
     gene            8553..9104
                     /locus_tag="LHL15_RS25760"
     CDS             8553..9104
                     /locus_tag="LHL15_RS25760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000662998.1"
                     /translation="MKEQKNFFERYKPVFEIVCRILGNGWRVNLLDDCQYRIKLTSPDF
                     KNYSIHIRMEKGRLVIIGSVDSRSWRSPYHTCTVSPERNPVEIAADIEKKILSDALDNV
                     DMAREYEQQLQQKREKKLILKGMLSRLVHLESWHGTLTGFKVENGLDGNVSERGDGYEM
                     VIRGLSVDQLIKVAGFIKQL"
                     /name="hypothetical protein"
     gene            9113..9382
                     /locus_tag="LHL15_RS25765"
     CDS             9113..9382
                     /locus_tag="LHL15_RS25765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001006717.1"
                     /translation="MNEVLSDRKNKGNVTYRIVVSEDATRLFIELEKLMKVRSKEADKK
                     TTKKIVFQKSFYYEEFSNVKDEIDDPILLKFYTDTIVKVQNHEF"
                     /name="hypothetical protein"
     gene            9372..9614
                     /locus_tag="LHL15_RS25770"
     CDS             9372..9614
                     /locus_tag="LHL15_RS25770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000008707.1"
                     /translation="MSFKPTPEEVKQARIKAGFTQQEAAERFGFTLSAWQAKETSGKTS
                     RGLAAGTYELLLLLADEHPDYQLVKREKNQDKVTK"
                     /name="hypothetical protein"
     gene            9708..10046
                     /locus_tag="LHL15_RS25775"
     CDS             9708..10046
                     /locus_tag="LHL15_RS25775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001099725.1"
                     /translation="MNTYVITFQALNTKKEKITCSAFVHSETLFLAVHSFEDKNRGRGY
                     VITSVSELLSEELTAKNSLKKNINFWFNECGLSKSEVINEVINWKNFAYTLKELEEAKN
                     EAIRELRS"
                     /name="hypothetical protein"
     Typing          complement(5318..6189)
                     /name="IncX3"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 gggtcttccc ttgttgtggt ggctgaaggc atgataatgg tgtatttaat cgccagaggt
       61 caccgccatg gacgaaaagt ccctctacgc tcatattctc aacctgtccg atccgtggca
      121 ggtaaagtcc ctttctctcg atgaaaatgc cggttctgtt actgtcacta ttgagatcgc
      181 tgaaaacacc cggctagcct gtccgacctg cggtaaatcc tgttctgttc acgatcaccg
      241 tcatcgtaaa tggcgccatc ttgatacctg ccagttcacc actattgttg aagccgatgt
      301 tccacgaatt atgtgtccgg agcatggctg cctgacgttg cctgttccgt gggctggccc
      361 cggaagccgg tatacgttgc tattcgaatc gttcgttctc tcatggctga aaatcagcac
      421 cgttgatgct gtcaggaagc aacttaagct cagttggaat gcggttgacg gcattatgac
      481 ccgggcagtt aagcgaggtc ttgcccggat aaaaaagcca ttatccgccc gtcatatgaa
      541 tgtggatgag gtcgccttta aaaaaggaca tcgttacata acggtgatct ccgatcgcga
      601 tggtcgggcg ctggccttaa cggatgatcg cggcacagag agtcttgccg gctatcttcg
      661 cacgctcact gatgggcagt tgctggctat caaaacgctc tcaatggaca tgaacgcggg
      721 ctatataaga gcagcgcgta tccacttacc cagtgcggtt gagaaaatcg cctttgaccg
      781 cttccatgtg gcgaagcaac tgggcgaggt agttgataaa acccgtcaga atgaacatcc
      841 gcacctccct gttgaaagcc gacaccaggc aaaaggaacc cgcttcctgt ggcagtacag
      901 cgataagtgg atgaccgaat cccggcagga aaagctgatg tggctgcgtg cacagatgaa
      961 gctgacgagc cagtgctggg cgctgaaaga gctggcaaag gatatctgga acaggccatg
     1021 gagcgaggaa agacggagtg actggcagag atggttggcg ctggcggcta acagtgacgt
     1081 tcccatgatg aaaaatgccg cgaaaacgat aggaaaaagg ctgtacggga tcctgaatgc
     1141 gatgcgacac agtgtctcaa acggaaatgc ggaggcactt aacagcaaga tcaggctgct
     1201 gaggataaaa gccaggggat accgaaaccg ggagcgcttt aaactggggg tgatgttcca
     1261 ctacggaaag ctgaatatgg cgttctgagc cttcccacca tgatcgggga agacccatgc
     1321 atatacagta taaagaatca gttttattct tcaacacact gtttattatg ttgtaactac
     1381 tccctacctt caggttcatg gttcattttg ttaacggtct gttgcttcac agctttgctg
     1441 atagtcgccc ggctacaacc cagcactttt tgaatctggc tccaggagct gccgctcgca
     1501 atcagcctgt ttatggcgtc atagcgagac tgattaacct ggcggccttt atacttccct
     1561 tcccttttcg ccctggcgat cccctgctgc tgccgttccc ggcgctgttc atagtctcgc
     1621 ctggcgacgg cggccagcat atccagcaac atatcgttta tggccgaaaa cattcggctg
     1681 tcaaaatcat tgtgacccga tgcgagccag gttgtcggaa cgttgacggc cacaacccgg
     1741 atatctttct gccggatcat tttcttcagt gtgttccagt cctcccctac caggcgtgaa
     1801 agcctgtcca catcctctac cagcaagata tcgttttgct gacaatcttt caggagccgg
     1861 aacaactcag ggcgttcaag cttagaacct gattcatttt caatgtagta gttacagatg
     1921 ctcaggccgc gctcgatggc gaaggtattt atcgtgtcca gggcgcgtgt tgcatcctgt
     1981 tcagtcgtgg atgcgcgtaa gtaagctctg acaaagcctt tcggtactgt ttgagtcatt
     2041 atagggaacc agttcatttt gagttaacca gacacaccca tacagtaccc atttcagttg
     2101 gttcagggaa ggcacactca aaaagaacca gcaaacagac cccagcccta tagtgacaac
     2161 gtcacactaa aaatttacaa cataaagatg atgtaaagac acaccaagat ttacaccata
     2221 aagcgcacat aaagacacag caacatttac catgtaaaga caccataaag ataaatatat
     2281 ctttatttgt gctttacatg gtaaagccat tgcattataa agacactgta aagacaaaag
     2341 tatctttacg aaaaacacac catgtaaaga cattcatatc tttacatgag ctttacaaca
     2401 taaagagagg gattctgaaa tgggtaaaat cttgcttgtc gtatcagata aaggtggggt
     2461 aggcaaaagc acctacgtgg ctaacacagg ctcaatgctg gtcaacaaag gtaagtcggt
     2521 aattatcctg aagacagata aaaaccacga tctgttgagc tggaacgaaa agcggacaga
     2581 taacggctta ctcactatcc cggttcacgc agcttacgga aacgtcagca acgaaattaa
     2641 gcgccttagt aagctgtgtg aagttctcat tgtcgattgc cccggccacg acagccagga
     2701 gttccgtagc gccctgacag tatcagatat cgtggtgaca ctggttaagc cgtcctctga
     2761 tttcgaaagc gaaacactga ccagtgttac agagaaaatc cgcaccgctc agaaagctaa
     2821 cgcagctctg gaaccgtggg tgctgttcac cagaatcaac tcagcgaaac cccgccacag
     2881 aaaagcggcc attgatctgg ataaattact gcgttcggac aacatctgga ttcaacctct
     2941 gaaaacccgg atatccgagc tggatgtgta tgaaagcgcc tgtaatgaag gggcaggggt
     3001 acatgacgtt agccgggcat cgagcctttc aacagcgaaa gcacagattg aacttgtggc
     3061 acaggaaatt ggcattttat agaaatatca acaatattca tcgaaatctt tacattgtaa
     3121 atacaccata aagatactat gatatttaca attaatgcat accgtacaag gggaaatttt
     3181 catggcttta aaactaaacc gcccgaacat cgatgaatct cagcaaccag caacaaacgc
     3241 agaaaccgcg cgttttatct ccggcgctac cagagcgcct gtagcgggta aaccaaagtt
     3301 gatgaacttc agactgccag cgtactttga ggagatcctg gaagcggagg cgcagagaac
     3361 aggccagagc aagacaaccg ttctaaaggc agcgctggcc gcattcgata acctggacga
     3421 gaacctgaaa aaccactggc tgcttgaatc tgcaaaatta gggtagcgtt atggctaaga
     3481 aaaagagata tgttgtgatg gttagagata aaccaaatac agcggcaatc agcgattatt
     3541 agcctggtta gtatggttcg ctaacaggca taataaggcc gttgcctatg actgcggaga
     3601 atggattgtc gaaccatcac attggttaag aattggaaat ccaaaataga aagaaaccta
     3661 aggaaagaaa aatgttcata ttcagatata ttaaaactct tattaatatc tacttgttat
     3721 acatccaacc gatattgggg atgttcctta taggtatagg cttatggggg ttctatgcac
     3781 ctgatgaggg aacttgtact aatctcaata ttgagttatg cgctgtatca gctattgatg
     3841 ttctgaagcc tgctattttt gtatttttag gcattattac cgttgccgga tggtacttta
     3901 ccgaacttca gccgaagcta aggggggagg gcaagtatat gaaggtaaaa acaggtgtaa
     3961 aaccggaaga tttcgttaag taaaaaccga gcctaaagac gttaaccgtg ggggatttat
     4021 gaataaatca tggcttgaac aacaggtgca aaatgaaaaa ttaaaaaaag tacaggaaga
     4081 gaaaaaagat ggcactatcc aaaaaggatt gtacagggtt cgtataaaag acaaatacag
     4141 gtgggatttg tgggaaaatg atttcagagt tgttattgct tattcgaaag atgatgcaga
     4201 gctaaaattt acagaaatga caggttttaa tgagcattac tatatagaaa gcattgaaaa
     4261 aatagtgctt tcagatatag aaaaaatctt actggaaaac tgaaaagata gttatccaga
     4321 ggtttagtta aataatggcg tcagtttttc aaccagtagc agatcgctta aaccgtctaa
     4381 agaaacaact tctataccag aatcaacaag tgaatcaact ccacatactt caccgaagcc
     4441 gataaccata atcggctttc ggtctatcaa atcaaaaatc accaaaggca atattacact
     4501 gttaatcgct tcaaactcct gaaaaaacat ttcagagccg tattcgctgg caggaaaaag
     4561 aatctcactt aatctcaaac cataaaaaat atggtagtgt tctttaaggt tatcctttaa
     4621 aatattaaca gccttagaat atttttcgtt aataaactct ttaacctgat atttcataac
     4681 tctctccaac aagaaccgac tgtaggtcac cgggcaaacg ttgcggaatg gcgtcagaga
     4741 cgtcattttg cggcgtttgc cctatcctgc atcgcagtga aaagaactcg cctcgtgcgc
     4801 tatgattaac cgatgataaa aggctgaaag aagcactgca agccgactgg ataacattgt
     4861 tgcttcacgg ctgatcatca ttggttgcct cagtgctagt gtgctttgca tcaggaacca
     4921 gaagacgata ccacggcatt tttttgttaa gcagctccat caggggagtg gcaaacaaac
     4981 tcaatcccca cagcgttacg attactggaa tcgtgaaatt ttgcggcgtc cagaactggc
     5041 gctcaaggtg gaaccatgta acaacacaga gcattccacc aatccagaaa atagctttac
     5101 tgaaagcccc caggactgcc agagaaataa cagagactaa atgcagtgcg ccagccgtag
     5161 caagacgtag ataaaaccag aaccatttag cgtgccggat aaccgggtta ccgttcgttt
     5221 ctcgcaatcg ttttttacgt tcccggatga tttctttttt cagcttactt cgttcggctc
     5281 cctgcggaaa ctcatatatt ttagccatgt tttgcctcac ttattctttt ttggtggaac
     5341 gactttgtca aactcctcta aaaatgccat atcatcttcc gaaaaggatg aggatttttc
     5401 atttatacta aactcaaatc taagcatgtg agccattctc ccttttttcc caacaacaga
     5461 gaaagataac tgatttattt ccgttttctc aattatctca ttaatgcttt tattgataac
     5521 atcacgaaca aaaaatgagt atttaggata cttatatttc ttctcccctt cctcaatagt
     5581 ataaagtccc atttcctctt ttaaatcatc cacggatata tcaaaagatt tttttgaact
     5641 attctgactg taatatttcc taatgacttg ataaaggttt gttgagttta cactgttcaa
     5701 caacacaact gatttcaata cctgagtggt atatctattt tcagaaccaa tcaacttaga
     5761 aaaatatcgc ttagctgtat ttgtgaattt tatccgtaca gttgcaagat gatcgtaata
     5821 ggcacagaat tctgttaagt ttaaatccaa gcgatcaggt attttattct tagatgatag
     5881 tatcccaagt tcatcagcca aattttttaa atcatctctg tttaaaacta acgtagttga
     5941 agaaagaact ttagctccct ctttcagagc tgcataagag gcatatttat ctaaagaagt
     6001 agcttttgaa aaaaagtcag catcaacctc caaaatatca tcatctaaca tttcctttgt
     6061 atcaatctgg caaagagcca gaaaaagcac tctttttgct gaaagggaaa gggatgcaag
     6121 agttacccca acttcatttc tatgtctaac tttcgttaat cccgttactg tcttcacgat
     6181 aagcctcata taacctttcc tatgtaggca aatcataacc ccacaaaggt tgtaattcaa
     6241 gacaacaata caacctttaa ttaaaaaacc tctcaccttt agatatgaag atctgaggat
     6301 cttcatatct aaagatagca acaaccgcat gtttcacttg atgttcagtc aaactgtgga
     6361 aaccgttaaa aatcttctca ccttacgtga aaaatcctct catctttagt gaaaaatcta
     6421 actaccttaa cttaaaaatc ttctcacctt acgtgaaaaa tcctctcacc ttacgtgaaa
     6481 aatcctctca cctttagcaa tttttttcca taattttcag gtaattgaag cgagctaagt
     6541 ttttaagttt ttaagatcaa gaagaaaata taaggctttt aatgcacagc gtctgtggat
     6601 aactcagcat aaatccagag gatagatccg tctggcctcc ctgagcactg caccaccaaa
     6661 aattgagcaa atattaacct tagcgcgtaa gtgctctatt gaaaccccgt ggaaattccg
     6721 cttcctcgct cactcgcggg cttcgccctg tcgttcggct gcggcgagcg gtgtaatctc
     6781 tctaaaagat ccagacggga tccttttccg atccaattct taaatgctct caaaagccct
     6841 cagaggcgtt ttaaccgctt taacccggtc atggttcaac ggagcgggta aaatgcgctt
     6901 agaacgtcat acagctcgtt tttaacctga tccctaatca gggggtataa cagaggcggc
     6961 caaaaaatcc ggccttgctc gtcagagtat ctaccgcgca ttatccccga acggaaaccc
     7021 gaccatcaca acacttgcac agcttgcagc agtagccggg ttacagttca ctctcagtaa
     7081 gcccaaccac taacctttca gacaagcccc tcataccggg gggattattt cttacgccag
     7141 caaaatttca cgaccggcga ggatggccaa gtgttttttg ctggcaattt tcgtgcagca
     7201 gctggcgccc ggattcattt tttgctgtca ggcatcaatc ggtttcagca acccgccccg
     7261 taattcgctg ctaacgcatc tcataacgcg cagatacagc gccccgcgcc agtgtttcac
     7321 actgacccgg cgtaccgttc gcaagctcac gcgctaactg cgacaggcta aacatcgtct
     7381 gatagtcaga aattggcctg gcaggggtga ggggggttat cgctctcatt cgggcttgtt
     7441 ggttcgccag tcctggcgaa cgtctccggc gcgatgttac gacagcgcat tatgtaacta
     7501 cagttcgcgc ctctttcgga aatcccttca tgcgcggtaa agcaatatac atctttatgt
     7561 aatctttaca atgtaaagca ttaaaaatga ttccataacc atcatttgat ggtataatga
     7621 atttgttggt tgggaatggt tccctgccag attccagaag cccacacggg cgggagcgaa
     7681 aaatgaatat tcaggaagca ttaaacgttt ttggtttatc cggtgatctg actgaaaagg
     7741 acatcaaggc ggcatacaaa aaagcggctt taaaatatca tccagaccgc aatccattgg
     7801 gtgccgagct gatgaaagca gtaaatgctg cgtttgattt tctcatggct aacattgata
     7861 aaatcaatca gtttcaaagc actgatgaaa acgcacgtta caactacggt gaagatctgg
     7921 aaaaagttct gaatacgctt tccggcctta cagggattgt ctatgaagtt attggtaatt
     7981 gggtctggat tagcggagaa actaaagaac acaaagacat tttaaaggaa atgggctgta
     8041 agtgggcatc taagaaaaaa caatggtttt accgtcctga agaacacaaa agccgctgga
     8101 accgtaaaga acacagcatt gaagaaattc gtgaaatgta tggtacagcg ggtaaacgta
     8161 aggcgtcagg ctggacacgt gtagaagcaa gcgcataacc ggaatggggg cgaaagcccc
     8221 ctagaacatg gcaaagattg attcggagac acgttgatga aaaacaagac cataacggaa
     8281 gcggagctga taaacatttt tgaaagttac ggagcctaca tttgcccgga tgagatcgaa
     8341 gttacagcca aagaatgcaa tgaaaatggt tcagtcttgc accgtggctt aaacgcagaa
     8401 ggatgggcgc atctttttgc gaaggaagaa gcatatcagc aggaatgcga agcccaggaa
     8461 gctgcaagtg atgatggaca ctttgatgaa tagtcttaaa actgccgtaa ttcgatttac
     8521 ggctttttta ttgagttcaa aaggctgaat cgatgaaaga acagaaaaat ttctttgaac
     8581 ggtataaacc ggtgtttgaa atcgtttgtc gaatcctggg gaacggctgg cgtgtaaatc
     8641 tgctcgatga ttgccagtac aggattaagc taacctcacc tgattttaaa aattactcta
     8701 tacatattcg aatggagaaa ggaaggttag ttatcattgg aagcgtagat agtcgcagct
     8761 ggagaagtcc ttaccatact tgcacagtat cgccagagcg taaccctgta gaaatcgccg
     8821 ccgatattga aaagaaaatc ctgtcggatg ctttagataa tgtagatatg gcgcgagagt
     8881 atgaacaaca gttacagcaa aaacgcgaga aaaaactgat cctgaaagga atgctatctc
     8941 gtttagtgca tcttgaaagc tggcatggga cattgaccgg gtttaaagtt gagaatgggt
     9001 tagatggtaa cgtatcagaa cggggcgacg gatatgaaat ggtaattcgt ggcctgagtg
     9061 tagatcagct cataaaggta gcggggttta ttaaacagct atgaggcaaa aaatgaatga
     9121 agtcctgagc gacaggaaaa acaaaggtaa tgtaacatac aggattgttg tgtctgaaga
     9181 tgcaacacgt ttgtttattg aacttgaaaa actgatgaag gtacgctcta aagaagcgga
     9241 taaaaaaaca actaaaaaga ttgttttcca aaaatcattt tattacgaag aattctcgaa
     9301 cgttaaagat gaaattgatg atccaatttt attgaaattc tatacagata ctattgtgaa
     9361 ggtacaaaat catgagtttt aagccaacac ctgaagaggt gaaacaagca cgtattaaag
     9421 ctggctttac gcagcaggaa gccgctgaac ggtttggttt tacgttatca gcctggcaag
     9481 ccaaagaaac atccggtaaa acgtctcgtg gtttagccgc tggtacgtat gaattacttt
     9541 tgctcctggc tgatgaacat ccagattacc agctcgttaa gagagaaaaa aatcaggata
     9601 aagtgactaa ataaattgca ttaccatcat atgatggtat aatttatttg taggtcggga
     9661 atagttcctt accatgatcc agtagcccaa acgggcggga gactaaaatg aacacgtatg
     9721 ttatcacttt ccaggcttta aacactaaga aagaaaaaat cacctgctct gcttttgtcc
     9781 attcagaaac tctcttcctt gccgttcatt ctttcgaaga taaaaatcgc ggtcgtggtt
     9841 atgttatcac gtctgttagt gaattgttgt ccgaggaact gacggcgaaa aattccctca
     9901 agaaaaatat taacttttgg tttaatgagt gtggtttaag taaatctgag gttataaacg
     9961 aagtaattaa ctggaagaat ttcgcatata cccttaaaga actggaggaa gccaaaaatg
    10021 aagcgataag agaactgcgg agttaatcaa ttaggggtgg tatcaggttg tggaggcaac
    10081 ctgataccgt gacactcaat tccctcgcga ggaatataaa atgtcggaac gcatgttatc
    10141 agcaatacag actgttgaaa agggtgggcg tccggttttt cccttgatgc cattctctgc
    10201 ttttcctgag tacatggcat tactcag
//
