LOCUS       NZ_CP084865             2317 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB1067 plasmid pKp_SB61067_5, complete
            sequence.
ACCESSION   NZ_CP084865
VERSION     NZ_CP084865.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024791
            Assembly: GCF_020525845.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB1067
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:21:35
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,441
            CDSs (total)                      :: 5,314
            Genes (coding)                    :: 5,177
            CDSs (with protein)               :: 5,177
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 15
            Pseudo Genes (total)              :: 137
            CDSs (without protein)            :: 137
            Pseudo Genes (ambiguous residues) :: 0 of 137
            Pseudo Genes (frameshifted)       :: 54 of 137
            Pseudo Genes (incomplete)         :: 90 of 137
            Pseudo Genes (internal stop)      :: 18 of 137
            Pseudo Genes (multiple problems)  :: 22 of 137
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084865.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB1067"
                     /isolation_source="feces"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB61067_5"
                     /geo_loc_name="Netherlands"
                     /collection_date="2002"
     gene            complement(join(2225..2317,1..444))
                     /locus_tag="LGM10_RS27145"
                     /old_locus_tag="LGM10_27145"
     CDS             complement(join(2225..2317,1..444))
                     /locus_tag="LGM10_RS27145"
                     /old_locus_tag="LGM10_27145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002069891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023150440.1"
                     /translation="MKKVYTAIILIVLLCGGVLSANYIFLQRHMNEVLKEDPRNDGISV
                     WVYYKWFVNSSEINYDLRSVSAENSSLDVSRVMLQFAEKVKDYDFSKVYLSYRGKDKFY
                     LKGEYFKTLGQEYGIQNPVYTLRTIPENVYMLNGERAYSVWEGGLLGVMGKQMEDLSDF
                     SKAWYLDDFIKSMSD"
                     /name="hypothetical protein"
     gene            complement(733..969)
                     /locus_tag="LGM10_RS27150"
                     /old_locus_tag="LGM10_27150"
     CDS             complement(733..969)
                     /locus_tag="LGM10_RS27150"
                     /old_locus_tag="LGM10_27150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947370.1"
                     /translation="MKRLINLTPAEKRFLDDAVAAAERASGKKLNQPNRHIVLNRARAQ
                     IESQRQAERQRSAREEERQQAEFTWSRPRAPRR"
                     /name="hypothetical protein"
     gene            1731..1844
                     /locus_tag="LGM10_RS27305"
     CDS             1731..1844
                     /locus_tag="LGM10_RS27305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071789001.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGN"
                     /name="replication initiation protein"
     Typing          complement(1200..1378)
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="84.066"
                     /coverage="83.0"
                     /evalue="3.53e-41"
     Typing          1355..1844
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="94.0"
                     /evalue="0.0"
ORIGIN
        1 cccaccctcc caaacactat aagctctctc tccatttaac atatacacgt tttccggaat
       61 tgttcttaat gtatatacgg ggttttgaat accatattcc tgcccaagag ttttaaaata
      121 ctctcctttt aagtaaaact tatctttccc tctgtaacta agataaacct tactaaaatc
      181 ataatctttc actttttcag caaattgcag catcacccta ctaacatcca gtgaactatt
      241 ttctgcagaa acagaacgaa ggtcataatt aatctcggaa gagtttacaa accacttata
      301 ataaacccaa acgctaatac catcattcct tggatcttct ttcagaacct cattcatgtg
      361 cctttgaaga aaaatgtagt tggcagataa aacaccacca cataagagta ctattaatat
      421 gattgctgta tatacttttt tcatttctat aatccatctc aactgctcta aagtttaccc
      481 tgagattttt aagctaaggt gccataagtt agcaaggaat taagcatcga tttaccataa
      541 cgtcttctta cgcgcaccgc gaaaacggcc tcagggcgtt tctgcgcctt cagccacaaa
      601 agagcacgtc ttacccccag aaaaccgccc ggaatcgctc tggtgagttt tacggtggcg
      661 aatgactatg caccggagtg aataccacgc tgtttcgccc tgaactcccc acaaaaccga
      721 cgaaagtggc aatcagcgac gcggcgcccg cggacgcgac caggtaaatt ccgcctgctg
      781 ccgctcctcc tcacgcgcag atcgctgccg ttctgcctgg cgctgcgatt cgatctgtgc
      841 acgagccctg ttaaggacaa tgtgcctgtt gggctgattc agcttcttgc ctgacgctct
      901 ctcagcggcg gcaacagcat cgtcgagaaa tcttttctcg gcaggcgtca ggtttatcag
      961 tcgtttcatg gcgttctccg gtcagaataa ctctgcacag tctgccagac acggcgaacc
     1021 tcatgaagaa cctgcagccg ggaacggccc tgagaaacgg tgaacgggaa cggattttaa
     1081 tccggtgccg tgaagcgttg gcggggtatc ggggcgcagc cctgagatat cgttttggtg
     1141 ccggcatcat gatggcggta ctccaaaaac acatcttgtc ccgctttcag tttctgaatt
     1201 tgcgggttcc gggcgcagcg ccggaccagt cacgtagcgc tagcggagtg tatactggct
     1261 taatatgtta gttgggcgtg gtggctcagg gaagtgcttc atgtggcagg ggaaaaatgc
     1321 ggctgcggtg cgtaagtgca aaatgctgat gatggtgaaa tgacgcctcc tcgctcactc
     1381 ggtcgctacg ctcctgccgt gagactgcgg cgggcgttac ctgctcacga atgacgaggt
     1441 aacgaaatga agctgaggtc aagaagggcc cgaaaaagga tgcggcgctg ccgtttttcc
     1501 ataggctccg cccccctgac aagcatcaca aaatctgacg ctcaaatcag tggcggcgaa
     1561 acccgacagg acttaaagat cccaggcgtt tccccctggt ggctccctcg tgcgctctcc
     1621 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tatctcattc
     1681 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     1741 cccccgttca gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1801 taagacatgc aaaagcgcca ctggcagcag ccactggtaa ctgatttaga ggagatagtc
     1861 ttgaagtcat gcgccagtta aggctaaact aaacgaacag ttttggtgac tgcgctcctc
     1921 taactagcca gttacctcgg ttcaaagagt tgctgctctg agaaccttcg agaaaaccgc
     1981 cttgctgggc ggttttttcg tttacagagc aagagattac gacgcaacag aaaggatctc
     2041 aagaagttcc ttgctgtatt gctactatcc tagagatctg acccattagg ggcattatca
     2101 gatgacggcc ttgtaatcca cggggagcag gtcacacatc taaagcatct acgagtaaaa
     2161 acaccccagt gaccaatgag cgatttgcgt gagctaatcg tcaatgtata ctgatcgctt
     2221 caaattaatc gctcatgctt ttaatgaaat catctaagta ccaagcctta gagaaatcac
     2281 taagatcttc catctgtttt cccattacac ccagaag
//
