LOCUS       NZ_CP084850             4018 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB611 plasmid pKp_SB611_7, complete
            sequence.
ACCESSION   NZ_CP084850
VERSION     NZ_CP084850.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024795
            Assembly: GCF_020525985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4018)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4018)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB611
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:20:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,656
            Genes (coding)                    :: 5,441
            CDSs (with protein)               :: 5,441
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 61 of 215
            Pseudo Genes (incomplete)         :: 163 of 215
            Pseudo Genes (internal stop)      :: 19 of 215
            Pseudo Genes (multiple problems)  :: 25 of 215
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084850.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4018
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB611"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB611_7"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            join(3915..4018,1..424)
                     /locus_tag="LGM15_RS28820"
                     /old_locus_tag="LGM15_28820"
     CDS             join(3915..4018,1..424)
                     /locus_tag="LGM15_RS28820"
                     /old_locus_tag="LGM15_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004883515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016246513.1"
                     /translation="MKCFWACSVSVVLAMSFTVAAAENNDNVTYTKPSAGRDLYLTCKG
                     YQVKGASVINVEKPSYLEGTGEQVEFAIITPVGASDGLKVFFDSWGFSSFGLIESHVMH
                     EHWSLVHVGGRASVKGEKIDYSFVDNQPQEGGSYKPKTGVFLYGCMNSDKRPSWVEKIY
                     TRTGVFRGGIED"
                     /name="hypothetical protein"
     gene            679..891
                     /locus_tag="LGM15_RS28825"
                     /old_locus_tag="LGM15_28825"
     CDS             679..891
                     /locus_tag="LGM15_RS28825"
                     /old_locus_tag="LGM15_28825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_034169353.1"
                     /translation="MGDFERTFGAGADFDRIIDENNSEYFREDRRKTKNSKKKFSSFQD
                     ALVWAKANPGKTIIRSPDGVGFIEK"
                     /name="hypothetical protein"
     gene            complement(1320..1433)
                     /locus_tag="LGM15_RS29030"
     CDS             complement(1320..1433)
                     /locus_tag="LGM15_RS29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007772075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_072035093.1"
                     /translation="MHEPPVQSDRCALSGNYRLESNPERHDKTPLAAATGN"
                     /name="replication initiation protein"
     gene            2081..2404
                     /locus_tag="LGM15_RS28830"
                     /old_locus_tag="LGM15_28830"
     CDS             2081..2404
                     /locus_tag="LGM15_RS28830"
                     /old_locus_tag="LGM15_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_024191642.1"
                     /translation="MLTMWVTEDEHRRLLERCEGKQLAAWMRQICLDEKPSRAGKLPSI
                     SPALLRQLAGMGNNLNQIARQVNAGGGTGHDRVQVVAVLMAIDAGLERLRHAVLEKGAD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            2613..>3047
                     /locus_tag="LGM15_RS28925"
                     /pseudo=""
     CDS             2613..>3047
                     /locus_tag="LGM15_RS28925"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            3082..3567
                     /locus_tag="LGM15_RS28840"
                     /old_locus_tag="LGM15_28840"
     CDS             3082..3567
                     /locus_tag="LGM15_RS28840"
                     /old_locus_tag="LGM15_28840"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_061390563.1"
                     /translation="MNSLLTLAKDLEQKSKAQQQTTGEMLKAAFSEHEKSVRAELSESE
                     KRISAAILDHDRKLSSAMSQRTKGMLRMISQTWLTIVLVSALLIASSAGILWWQGQQIL
                     DNYTTIQEQKSTQAMLSEKNNGVQLTTCGEERRRCVRVNPEAGRFGEDSSWMILAGK"
                     /name="MbeB family mobilization protein"
     gene            3571..3786
                     /locus_tag="LGM15_RS28845"
                     /old_locus_tag="LGM15_28845"
     CDS             3571..3786
                     /locus_tag="LGM15_RS28845"
                     /old_locus_tag="LGM15_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_072048928.1"
                     /translation="MTELEKQLLSALEQLQQDYSKRLDEWENAFAEWRTMSGLMQRENA
                     ALSERVTGLSRQVQSLSDQLRRLSQG"
                     /name="MbeD family mobilization/exclusion protein"
     Typing          1791..1968
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="87.222"
                     /coverage="83.0"
                     /evalue="4.5e-50"
     Typing          complement(1321..1841)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.564"
                     /coverage="99.0"
                     /evalue="0.0"
     Typing          2395..3878
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="91.717"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 cgggagggac ttatatctta cctgtaaagg ttatcaggtg aagggggcat ctgtaattaa
       61 cgtcgagaaa ccttcttacc ttgaaggtac tggtgagcag gttgaatttg caatcattac
      121 tcctgttggt gcgtctgacg gactcaaggt cttttttgat tcatggggtt tttcttcatt
      181 tggattgatt gaaagccatg tgatgcatga gcactggtct ttagttcatg ttggggggcg
      241 tgcttcagtc aaaggtgaaa agattgatta ttcatttgta gataaccagc cacaggaggg
      301 ggggagctat aaacccaaaa caggtgtgtt cctgtacggc tgcatgaact cggataaaag
      361 accatcttgg gtcgaaaaga tctacacaag aacaggtgtg tttcgagggg gcatagaaga
      421 ctaaaatact gcttacagca tgaaaatcca ttgaacagag tgtcagttca tttccgtgca
      481 taactatgca taccgtaaga acttacgggg ggcatttatc ggcgttttta cgtggtaaga
      541 ggtgctcttt tgcggttcat ggcgcagaac ggtgctgagg ctgtctgcgg cggtgcgaac
      601 aagggatgtt atggtaaata agagtgtctt ttggatattt taaatctgtt tttttattaa
      661 atttaatggg ggcagaaaat gggggatttt gagcgtacct ttggagctgg tgctgatttt
      721 gatcggataa ttgatgagaa taacagcgaa tattttcgag aagatcgccg aaaaactaaa
      781 aattcaaaaa aaaaattctc ttcttttcaa gatgctttgg tttgggcgaa agctaatcct
      841 ggcaaaacga taataagatc tccagacggt gtggggttta ttgaaaaatg aaagagtgga
      901 ttcactgtaa aacaacagtc attttttgga caacactgtc tatatacgta ctcatatgtt
      961 tcacagtgtc gtatgttgga gtctatgtta cgtacgttgc agggcctatt ttgttagtta
     1021 caggcattct tgcatggctt acgaaaccta gctgtaatcc aaagtgactg ggctagacga
     1081 actttatccc cccataaaac ccgctttttt aaacggatct tcttgagatc attcatggag
     1141 aatcgtaatc tcttagcact gtaaacgaaa aaaccgccca gcaaggcggt tttttcgaag
     1201 gttctcagag cagcaactct ttgaaccgag gtaactggct tggaggagca cagtcaccaa
     1261 aacttgtcct ttcagtttag ccttaaccgg cgcataactt caagactaac tcctctaaat
     1321 cagttaccag tggctgctgc cagtggcgtt ttgtcgtgtc tttccgggtt ggactcaagt
     1381 cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg catacagtcc
     1441 agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaattag ataaacgcgg
     1501 ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc gcacgaggga
     1561 gctaccaggg ggaaacgcct gggatcttta tagtcctgtc gggtttcgcc accactgatt
     1621 tgagcgtcag atttcgtgat gcttgtcagg ggggcggagc ctatggaaaa acggcaacgc
     1681 cgcatccttt ttccgccctt tctgattgat ttccgcttag tctctttatc cagccattcg
     1741 taagctggca acgcccgccg cagtctcacg gcaggagcgc agcgaccgag tgagcgagga
     1801 ggcgtcattt caccgggatc tgcatttcgc acttacgccc cacagcagca ttttctccct
     1861 gccacatgaa gcactttcct gaatccccac tccgatttac catgttaagc cagtatacac
     1921 tccgctagcg ctacgtgact ggttcagggc tgcgccccga aacccgctga aaagtactgc
     1981 cgcgccctgc gggcttgccc aacaccgctc cggacaggaa aaaatttccc gcccgaaccg
     2041 gtgttatccg gaggatggtt tggcagaaaa gcgcaacaaa atgctcacta tgtgggtgac
     2101 agaagacgag caccgccgac ttctggagcg ctgtgagggt aaacaactcg cggcctggat
     2161 gcgccagata tgcctggacg aaaagccctc gcgtgctggc aaacttccat ccatctcgcc
     2221 ggcgctgctt cgtcagcttg ccggcatggg taacaacctc aaccagattg cccggcaggt
     2281 taacgccggt ggcggtactg ggcacgatcg cgtgcaggtt gtcgccgtgc tgatggccat
     2341 tgatgccgga ctcgagcggc tgcggcacgc cgtgctggaa aagggggcag acgatgatcg
     2401 ttaagtttca tcccagaggc cgcggcggtg gcggcggtcc ggtggattat ctgctgggga
     2461 aagaccggca gcgcgacggc gccagcgttc tgcagggtaa gccggaggaa gtgcgggagc
     2521 ttattgacgc ctcgccttac gccaagaaat acacctccgg cgtgctgtct tttgccgaac
     2581 aggatttgcc gcccggccag cgtgaaaagc tgatggcgag ctttgagcgg gttctgatgc
     2641 ccggactcga taaagaccag tacagcgtac tgtgggttga gcaccgggac aaagggcggc
     2701 tggagctgaa cttcctgatc ccgaacacgg agctgctgac cggcagacgt ctccagccgt
     2761 attacgaccg cgccgaccgt ccgcgcattg atgcctggca gaccatagtg aacggcagac
     2821 tggggctgca cgacccgaac gcgccggaaa accggcgggc gctggttaca ccgtccgcgc
     2881 tgccggaagc gaagcaggaa gccgcccagg cgattacgcg cggcctactt tcccttgcct
     2941 catccgggga gctgaaaacc cgtcaggacg tcactgaggc gctggaaagt gcgggttttg
     3001 aggtggtgcg caccacgaaa agcagcatca gcattgccga ccctgacggg gggcgaaaca
     3061 tccgactgaa gggagccatc tatgaacagt cttttaacgc tggcgaagga cttagagcag
     3121 aaatcgaaag cgcagcagca gactaccggc gagatgctga aagccgcatt cagcgagcac
     3181 gagaagtctg tcagagcgga actgagcgaa agcgagaaga gaatcagcgc cgccatcctc
     3241 gaccacgacc ggaagctgtc ctcagccatg agccaacgca cgaaagggat gctgcgtatg
     3301 atcagccaga cgtggctgac catagtcctg gtctccgcgc tactgatcgc ctcgagcgcg
     3361 ggcattctgt ggtggcaggg gcaacagata ctcgacaact acacgaccat ccaggaacag
     3421 aagagcacgc aggccatgct ttcagagaag aacaacggcg tccagctcac gacctgcggg
     3481 gaggagagac gccgctgcgt gagggtgaac ccggaagcgg gaagattcgg agaggattcg
     3541 agctggatga tactggcggg gaaatagcac atgacggaac tggaaaaaca gttgctgagc
     3601 gcattagagc agctacagca ggactactcg aaaaggctgg acgagtggga gaacgccttc
     3661 gcggaatggc ggacgatgtc tgggcttatg caacgggaga acgcggcgct gagcgagcgc
     3721 gtcacgggct tgagcaggca ggtgcagagt ttgagcgatc agctgcgccg attgtcgcaa
     3781 ggctgaatgg catcgaggcg caccgggagc aggagcgcgc ggcgcagcac cagaaagcgc
     3841 tggagctgga acgatcgcag cggcagaaag gttttgagct tggaatgtag ttaatcaatg
     3901 aaaagggatg cgttatgaaa tgtttctggg cttgctcagt ctcggtggtt ttggctatgt
     3961 catttacagt cgctgctgcg gagaataacg acaacgttac atatacaaaa ccgtcagc
//
