LOCUS       NZ_CP084849            12069 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB611 plasmid pKp_SB611_6, complete
            sequence.
ACCESSION   NZ_CP084849
VERSION     NZ_CP084849.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024795
            Assembly: GCF_020525985.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB611
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:20:14
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,779
            CDSs (total)                      :: 5,656
            Genes (coding)                    :: 5,441
            CDSs (with protein)               :: 5,441
            Genes (RNA)                       :: 123
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 215
            CDSs (without protein)            :: 215
            Pseudo Genes (ambiguous residues) :: 0 of 215
            Pseudo Genes (frameshifted)       :: 61 of 215
            Pseudo Genes (incomplete)         :: 163 of 215
            Pseudo Genes (internal stop)      :: 19 of 215
            Pseudo Genes (multiple problems)  :: 25 of 215
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084849.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12069
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB611"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB611_6"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            complement(join(<11982..12069,1..>503))
                     /locus_tag="LGM15_RS28765"
                     /old_locus_tag="LGM15_28765"
                     /pseudo=""
     CDS             complement(join(<11982..12069,1..>503))
                     /locus_tag="LGM15_RS28765"
                     /old_locus_tag="LGM15_28765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /name="IS5 family transposase"
     gene            1778..1927
                     /locus_tag="LGM15_RS28770"
                     /old_locus_tag="LGM15_28770"
     CDS             1778..1927
                     /locus_tag="LGM15_RS28770"
                     /old_locus_tag="LGM15_28770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_164880199.1"
                     /translation="MRSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDSGPPVG
                     REAL"
                     /name="hypothetical protein"
     gene            2408..2599
                     /locus_tag="LGM15_RS28775"
                     /old_locus_tag="LGM15_28775"
     CDS             2408..2599
                     /locus_tag="LGM15_RS28775"
                     /old_locus_tag="LGM15_28775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000166328.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2599..3021
                     /locus_tag="LGM15_RS28780"
                     /old_locus_tag="LGM15_28780"
     CDS             2599..3021
                     /locus_tag="LGM15_RS28780"
                     /old_locus_tag="LGM15_28780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_021314599.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKDIKVID
                     TSELIRVFGSLDGVIDAVKHDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            <4141..>4212
                     /locus_tag="LGM15_RS28785"
                     /old_locus_tag="LGM15_28785"
                     /pseudo=""
     CDS             <4141..>4212
                     /locus_tag="LGM15_RS28785"
                     /old_locus_tag="LGM15_28785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743396.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element transposase"
                     /name="IS1-like element transposase"
     gene            complement(4305..5759)
                     /locus_tag="LGM15_RS28790"
                     /old_locus_tag="LGM15_28790"
     CDS             complement(4305..5759)
                     /locus_tag="LGM15_RS28790"
                     /old_locus_tag="LGM15_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="WP_226651249.1"
                     /translation="MTALVSSFKWVGIGQLVKIATQLVSLFYLTHLIPPSSYGLLAMSS
                     VVMNLASIFNDMGTGAAVIQRQEASIGFYNYIYRINIFTGLFVMLLVIITSPLIVVYFN
                     TPELYPVLFLLAFSFPISSLTITHKAKLEKEMNFKVIIAIEIFSSLTGMVLAIVLANLG
                     FGVYSLIFQTLTTISISTICFLKVSGLSLSLRIKNISHSDKSGVIGFSGYLLSFNLINY
                     FARNLDSILIGRFFSSAILGVYSIAYRIMLFPLQSLTFVFSRVFLPHLSKNINNTNNTN
                     KQDYLNALKLILSLSAPLMLGLSATSFSFTRVFFDPKWHLLGDLLHWLAPTAIIQSALS
                     TTGSVFMAYGKTRWLFFLGCIGAFLMGLSFIIGVHFDIKTLVYLYFFANLINSLPAFIS
                     LKNILKFSYIELVSVFFRTISPAFLMFLLIKVWQHFSFFKNASIDFSFNVALGIFSYIV
                     FYTLFNFNEVVRTGVIHKLRCMTKLK"
                     /name="lipopolysaccharide biosynthesis protein"
     gene            complement(6019..6987)
                     /locus_tag="LGM15_RS28795"
                     /old_locus_tag="LGM15_28795"
     CDS             complement(6019..6987)
                     /locus_tag="LGM15_RS28795"
                     /old_locus_tag="LGM15_28795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS903B family transposase"
                     /protein_id="WP_226651250.1"
                     /translation="MKDQITHLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLDD
                     ETIQAWYESATPSSRGRPQRYSELAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVPLR
                     CPDYSCVSRRAKSVNVSFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRIWRK
                     LHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHDELRR
                     KKISALIPPRKGAGYWPGEYADRNRAVANQRMTGSNARWKWTTDYNRRSIAETAMYRVK
                     QLFRGSLTLRDYDGQVAEAMTLVRALNKMTKAGMPESVRIA"
                     /name="IS5-like element IS903B family transposase"
     gene            complement(7118..8101)
                     /locus_tag="LGM15_RS28800"
                     /old_locus_tag="LGM15_28800"
     CDS             complement(7118..8101)
                     /locus_tag="LGM15_RS28800"
                     /old_locus_tag="LGM15_28800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_226651248.1"
                     /translation="MKKKFSIIVPVYNVEKYLRECIQSVLDQDFNDYEIICVNDGSTDG
                     SLDIILSFDDQRIKVINQENQGQSVARNVAIESSTGEYIWFLDGDDKISHGSLKLIDKI
                     TNEKNYGIIFFNAHVFFDTPDLKKKGWHDYKRSTFGEIDALTFFEKEMANKKLIVQPCC
                     YVFKRELLNGNAFIPHICHEDNHFIATLIFDNYSTICLSLDEELFLRRVRNDSVMTSKK
                     SMRHIIGYSTCIDELTKYKKTKGIQNKSFDRFVNGMVLSLFSISETIKTKEAMDYVARY
                     KKHPDLSFKDSVLINYPLIKKAYLKLKNGKVFNYLRNSIINKKSNP"
                     /name="glycosyltransferase family A protein"
     gene            complement(8274..>8405)
                     /locus_tag="LGM15_RS28805"
                     /old_locus_tag="LGM15_28805"
                     /pseudo=""
     CDS             complement(8274..>8405)
                     /locus_tag="LGM15_RS28805"
                     /old_locus_tag="LGM15_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
     gene            complement(8438..11335)
                     /locus_tag="LGM15_RS28810"
                     /old_locus_tag="LGM15_28810"
     CDS             complement(8438..11335)
                     /locus_tag="LGM15_RS28810"
                     /old_locus_tag="LGM15_28810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_049106386.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPSSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEVMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTLRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            11430..12035
                     /locus_tag="LGM15_RS28815"
                     /old_locus_tag="LGM15_28815"
     CDS             11430..12035
                     /locus_tag="LGM15_RS28815"
                     /old_locus_tag="LGM15_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_080901903.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATLLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          1569..1785
                     /name="MOB_unknown"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="86.301"
                     /coverage="100.0"
                     /evalue="4.44e-60"
     Typing          complement(1112..1623)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.204"
                     /coverage="99.0"
                     /evalue="0.0"
ORIGIN
        1 tttacccggt acatcgccgt ttccgctatc gagcgacggt tgtaatctgt tgtccatttc
       61 caccgcgcat tactcccggt cattcgctga ttagccactg cacggttacg gtctgcatat
      121 tcaccgggcc agtaacccgc accttttcgg ggagggataa gcgcgctgat tttcttacgc
      181 cgcagttcat cgtgacagag tcgggtatcg taagcgccat cggcggcggc tgacctgatt
      241 ttccggtggg tttgccggat taacccgggg aaggcctctg agtccgtaac gttgttcagc
      301 gacaggtcag cgcagatgat ttcatgtgtt ttactgtcaa cggcgagatg caactttcgc
      361 cagatacgac ggcgttcttt gccgtgtttt ttgactttcc attcgccttc accaaagacc
      421 ttcagcccgg tggaatcaat caccctgtgt gcgatttcac cccgggggaa cgttttgaaa
      481 ctgacattaa ccgactttgc ccggattaca gcagtgtcag caagcgttac gcgcatcgtg
      541 agactaaaca agccatatca gataacagct cttctttatg acccttttct tttttcatca
      601 cgcctatgaa tgtatgacat aggggtcggt ttgggaaacg taaattattg tacgttaaga
      661 acataattta accgtttctg attggtaact gattgattta ttgtcactga ctatcgcatt
      721 taagttattg cgctcacgct gtcgcattta ggttgtctca ttacccaaac atacaggcta
      781 aatgccgtat agtgcaggag tacaccctat tgcgatagcg cgatctgttc aaaaaacagg
      841 cttaacgtac aataatttcc gatctccaaa ctcaccccac atatgtgcat cggctttaat
      901 taatttgacg gcgttgctac accggtgcat tatccatgaa acgaaaaaac cacccggcaa
      961 ggtggttttt cgaaggttca gcaaaccggc aggtttctga accgtggtaa cagtcttgtg
     1021 cgagacgtgt caccaaatac tgtccttcca gtgtagcctc actaaggcct ccacttcaag
     1081 aactctcgat acatctctcg cacatcctgc ttaccagtgg ccgttgccaa tggcgcattt
     1141 gcacgtctat ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcggac
     1201 tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc ggaactgagt
     1261 gtcaggcgtg gaatgagaaa ccgcggccat aacagcggag tgacaccggt aaaccgaaag
     1321 gcaggaacag gagagcgcac gagggagcta ccagggggaa acgcctggga tctttatagt
     1381 cctgtcgggt ttcgccgcca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg
     1441 cggagcctat ggaaaaacgg tagcgtctca tcctttttct gctctgtttt aatgccgtat
     1501 gcctttgctc ttatccgtgt gcaggtaacg cccgccgcag ccgaacagca ggagcggaag
     1561 cgactgagtg agcgaggagg cgtcatatca ccgtaacctc cattttctcc tgtacgccct
     1621 gcagctgcat ttttcccctg ccacatgaag cactttcatt acgtgttact catccgtaac
     1681 atattaagcc agtctacact ccgctagcgc tacgtgactg gttcagggct gcgccccgaa
     1741 acccactaac aacctctcag ctactggata tcaccccatg cgatctgaat atgatgaaca
     1801 actggattcg actgatacgc agcttcccgg cgaatatgac ctttttgatt taatggcgca
     1861 ggttactgaa gagaacctgc atgcccctgt ggattctggg ccgcctgtcg gccgggaagc
     1921 gttatagctt tttcgcttgc ccgcgtacgt ggtaatacgc cggtattaca ttttatttct
     1981 ttattttcat gtaattacag gtgattgtaa tacgttgcgt attacataaa taaacggaga
     2041 tttcagtttt tactttttaa ttcatgcagt tgtgatgaaa atcgcagctg cgttaggtgt
     2101 atgaccattt tgaggttgaa aaataatcaa ccatggcggg tgatttacgc aggttaaggg
     2161 gcgtcggtga caaaaaaacg tcattaacga cctcgttcat gctcgcatga acggattatt
     2221 tttccggact acaggtaaac tacagaaaga ctacaaaagc tgatgactta atcctgattt
     2281 ttagtgcgat gactacagat aaactacaaa ataactacag gattacctca gatccgttct
     2341 ctgaaccatt cgtacatccg acccccttac cggttttaca ctgagcactg atgctctgag
     2401 gtcccttatg acgaaacaac agaaaacagc gctgaacatg gctaaattca tccagaatca
     2461 gtctctgctc ctgctggaga agcttaacga gcttgatctc gatgctgagg ccgacctttg
     2521 tgagaaactc cacgatgatg cggagcatct tttccgtaca ctgtcctcga gactggatgc
     2581 attacaggat ggtaactgat ggccagagtg tctattagtg aagcagcacg gcttgttaag
     2641 gttagtcgcc ctactattta caagatgatt aacagtggta agttaagtta cacctctgtt
     2701 gtaaagcacg ggaaggatat taaagttatt gatacatcag agcttattag agtgtttggt
     2761 tctcttgatg gtgtaatcga tgctgtaaaa catgatgtaa aaagtgacgc agaatctaca
     2821 ggtattaaca gcgttggttt gcatgattta caacatagaa tcgctttact tgaagctgaa
     2881 aacgatggtc ttaaaggggc tgttaaggct cgagatgagc atattgattc ccttcgtcag
     2941 gctatgcagc ttctggagca caagcacgaa ccttcttcac caccccattc cccgtggtgg
     3001 aaattctgga aaaaatcctg atcccgccat gcttgagttt tcctctccgg ccatgccggg
     3061 gcgttcgtga accttcccaa aaatcgccag atctctgaac gctcctcaca ctaaaaatat
     3121 ccacacccca cgcaaaatta aatttttgca gataactcat taatatcagt cagttggttg
     3181 ttattaaatt acgcagtatt accttctgta aggtcaatcc aagtcgtcgg tgattcgtgt
     3241 acgacttgcc gacgactcgt catagagtca tcgacgactc actccacact atcagtaact
     3301 tatgacgact tgactctaga gtcgaatcgt cgctttggcg tttttatgac cgtggtgact
     3361 ctagagtcaa gtcacgagtc gaatcgccgg tgaatcgtgt ggtgagtcgt ccggcgactt
     3421 gccataacgt gattgccggc cgtcgacgaa cccgattcct gaacgtgctg acagcgggtt
     3481 ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc gttcaccgtt
     3541 ttcgggtaag aagaacatcg gaaacaggac ttaccccggc tgaatggcgt gaattttcgc
     3601 gctatgcact tgcgtgcata ctcatgcatg ccgtaaaaac agagtctgcg cgtttctggc
     3661 gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg ccctgaaagc
     3721 ctctcaggcg gtgctgataa cagatgttat ggtaaatgca agaatcttgg ctgtgatcca
     3781 aatagttgcg gccccccgaa gggggccgct tttggtggag ctaatgggac tcgaacccat
     3841 gaccaatgaa ttagaagttc actgctcttc caactgagct acaaggggtg agtttggaga
     3901 tcggaaatta ttgtacgtta agcctgtttt ttgaacagat cgcgctatcg caatagggtg
     3961 tactcctgca ctatacggca tttagcctgt atgtttgggt aatgagacaa cctaaatgcg
     4021 acagcgtgag cgcaataact taaatgcgat agtcagtgac aataaatcaa tcagttacca
     4081 atcagaaacg gttaaattat gttcttaacg tacaataatt tacgtttccc aaaccgaccc
     4141 cttggatgcc gggccacatc ccgcattatg ggcactgtcc tcaacacgat tttacgacac
     4201 ttaaaaaaat taggccgcag tcggtatcgg gcattatctg aacataaaac acaagcagta
     4261 agttggagtc attaccaatt catttaatga atttaataaa tgaatcactt taacttggtc
     4321 atgcacctta acttatgaat tactcctgtc ctcacaacct cattgaaatt aaacaaggta
     4381 taaaaaacaa tataactaaa aatacccaat gcgacattaa aagaaaaatc aatgctggca
     4441 tttttgaaaa aggaaaaatg ctgccatact ttaataagta agaacatcaa aaaagctgga
     4501 gagatagttc taaaaaacac gcttaccaat tcaatgtaac taaacttcaa gatgttctta
     4561 agtgaaataa atgccgggag tgaattaatt aagtttgcaa aaaaataaag gtagaccaat
     4621 gtttttatat cgaaatgaac acctataatg aaagacaacc ccatcaaaaa agcgccaatg
     4681 caacctaaga aaaacaacca ccttgttttt ccgtaggcca taaaaacaga acctgttgta
     4741 ctcaaggctg attggataat cgctgtaggt gccaaccaat gcaataagtc tccaagtagg
     4801 tgccacttag gatcaaagaa aaccctcgta aaagaaaaag atgtagcaga taaacctagc
     4861 atcagtggcg cgcttaatga tagtataagt tttaatgcat ttaagtaatc ttgtttatta
     4921 gtgttattag tgttatttat gtttttgcta aggtgtggta agaaaactct agaaaaaaca
     4981 aacgtcaggc tctgcaacgg aaataacata atgcggtagg caatcgaata gaccccaagt
     5041 attgcggatg agaaaaaacg gcctattaaa atagaatcaa gattcctggc aaaataattt
     5101 attaaattaa aagacaataa ataaccacta aaccctataa ctcctgactt gtcactatgg
     5161 ctgatatttt taattctcaa tgataaactc aacccggaaa ccttcaaaaa acatattgtg
     5221 gatattgata ttgttgtcag tgtttgaaaa atcaaactat aaacaccaaa gcctaaatta
     5281 gctaaaacta ttgccagcac cattcctgta aggctagaga aaatttcaat tgcaatgatg
     5341 actttaaagt tcatttcttt ttctaatttt gctttgtgag ttattgttaa gctggaaatt
     5401 ggaaagctaa aagccaatag aaatagaacc gggtatagct cgggtgtgtt aaagtacacg
     5461 acaatcaatg gggatgttat tattacaagc agcataacaa acaacccagt aaaaatgttt
     5521 atcctgtata tgtaattata aaaaccgatc gacgcctctt gtcgctgaat tactgccgcc
     5581 ccagttccca tgtcattgaa tatgcttgct aaattcataa ccacagatga cattgctaat
     5641 agtccatagc tcgatggggg gattaagtgc gtcaagtaga ataacgaaac caattgtgtt
     5701 gctattttta caagttgtcc aatgccaacc catttaaaac tactgactag tgcagtcata
     5761 ttgtttttaa tatccttttg aattttcaat aatttataat tcgtgatggt tttaaagata
     5821 ccaccataaa agacatcata attcttcaag ctatcatcta cgttgtagat tccgatttag
     5881 ctaaacttta ggactttata agttcgtatc ccgtatgatt ataaggcatt atgatcatct
     5941 ccaccaatag tgtactcgca acggctttgt tgaataaatc agatttcggg taagtctccc
     6001 ccgtagcggg ttgtgttttc aggcaatacg cacgctttca ggcatacctg ctttcgtcat
     6061 tttgttcagc gctcgtacca gggtcatagc ctccgcaacc tgaccatcgt agtcacgcag
     6121 cgtcagtgaa cccctgaaca gctgttttac ccggtacatc gccgtttccg ctatcgagcg
     6181 acggttgtaa tctgttgtcc atttccaccg cgcattactc ccggtcattc gctgattagc
     6241 cactgcacgg ttacggtctg catattcacc gggccagtaa cccgcacctt ttcggggagg
     6301 gataagcgcg ctgattttct tacgccgcag ttcatcgtga cagagtcggg tatcgtaagc
     6361 gccatcggcg gcggctgacc tgattttccg gtgggtttgc cggattaacc cggggaaggc
     6421 ctctgagtcc gtaacgttgt tcagcgacag gtcagcgcag atgatttcat gtgttttact
     6481 gtcaacggcg agatgcagct tacgccagat acggcggcgt tcctggccat gctttttgac
     6541 tttccactcg ccttcaccga agaccttcag cccggtggaa tcaattacca ggtgtgcgat
     6601 ttcaccccgg gtgggcgttt tgaaactgac attaaccgac tttgcccgcc tgctgacaca
     6661 gctgtaatcc gggcagcgta gcggaacgtt catcagagaa aaaatggaat caataaagcc
     6721 ctgcgcagcc cgcagggtca gcctgaatac gcgtttaatg accagcacgg tggtgatggc
     6781 gagctcagaa tagcgctgag gtcttccccg tgatgaaggc gttgccgact cataccaggc
     6841 ctggatagtt tcatcgtcca gccagaaagt tatggagcca cggttgatga gggctttgtt
     6901 gtaggtgggc cagttggtga ttttgaactt ttgctttgcc acggaacggt ctgcgttgtc
     6961 gggaagatgc gtgatctgat ccttcaactc agcaaaagtt cgatttattc aacaaagccg
     7021 cgtatcgcct gaaaacccaa cccgtcacgg ggatacctat ccgaaatctt atttattcaa
     7081 caaagaaaaa actttattaa ttatcgcttt gcttttatta agggttgctt tttttattaa
     7141 taatgctatt ccttaggtaa ttaaatacct tcccgttttt tagttttaaa taagcctttt
     7201 taattaatgg gtaattaatt aatacgctat ctttaaatga taggtcgggg tgttttttat
     7261 atctcgccac ataatccatt gcttcctttg tttttattgt ttccgatata gagaataacg
     7321 acaaaaccat tccattcaca aatctatcaa agctcttgtt ttgtatgcct tttgtttttt
     7381 tatacttcgt aagctcatcg atgcatgttg aatatccaat gatgtgcctc attgattttt
     7441 tagatgtcat tacggaatca tttcttaccc tcctaagaaa taactcctca tccaacgaga
     7501 ggcatatagt tgaataattg tcaaaaatca gggttgctat aaaatgatta tcctcatgac
     7561 atatatgcgg aatgaatgca ttgccattta ataattccct tttaaaaaca tagcaacatg
     7621 gttgcactat tagttttttg ttcgccattt ctttttcaaa gaaagttaat gcatctattt
     7681 cgccaaatgt tgagcgctta taatcatgcc agcctttttt tttcagatct ggagtgtcaa
     7741 aaaaaacatg tgcattaaag aaaataatcc cataattttt ttcattggtt atcttgtcta
     7801 ttaactttag acttccatgt gatattttat catcaccatc taaaaaccat atatattcac
     7861 cagttgatga ttcaatggcg acatttcttg ccaccgattg gccttgattt tcctgattta
     7921 ttactttgat tctttggtca tcaaatgaca gtatgatatc tagactgcca tctgttgaac
     7981 catcatttac acagattatc tcatagtcgt taaaatcctg atctaatact gattgaatac
     8041 attcccttaa gtatttttca acgttatata caggtactat tatcgaaaat ttttttttca
     8101 cagtaatctc caaataatat ttagttatac taacatcaag ttgttattta tggaatgcga
     8161 tttatcaacc tgacagagct aaggtgatga tgccaatttg ctcatttagt gtctaatgga
     8221 ctttgttgaa taaatcagat ttcgggtaag tctcccccta gcgggttgtg ttttcaggca
     8281 atacgcacgc tttcaggcat acctgctttc gtcattttgt tcagcgctcg taccagggtc
     8341 atagcctccg caacctgacc atcgtagtca cgcagcgtca gtgaacccct gaacagctgt
     8401 tttaggggtc ggttccggct gagggcgaaa tgacacccta agctttcggt tccttgggcc
     8461 aaagatattc gccagtcagt agaatgtgcg cccagcccaa tggggatatg tggggaagaa
     8521 attcaggggg aacatccaac ccttcgttcc gccgctccgt gacggcatga ccaagatgga
     8581 cggtattcca gtaaatgatc accgcagtca ataaattgag cccagcgatt cggtagtgct
     8641 gcccctctgt cgtgcgatcg cgaatttccc cctgcctccc gatacggagc gcatttttga
     8701 gcgcatggtg ggcctctccc ttgttaagac cgatctgagc acgccgctgc atgtccgtat
     8761 ccaggatcca ctcaataatg aaaagggtcc gttcaatacg accaacttca cgaagcgcaa
     8821 ctgcaaggtt gttttgtcgt gggtaagaag cgagcttgcg caggagttgg ctgggcctga
     8881 ttttgccagc ggtcatcgtc gcggcacaac ggaaaatatc aggccagttc gcaacgataa
     8941 gatcctcccg ggcttttcca cctaccaact tgcgtaactc cctgagggtc gtatcgggat
     9001 taaatacgta caaccgcttc gatggcagat ccctgattcg cagaacgaga ttgtagccga
     9061 gcaggctact ggctccgaac aaatggtcgg tgaatcctgc tgtatcggca tactgttcgc
     9121 gaacatggcg accgacctcg ttcatcagta gtccatcgag aatatacggt gcctcgctca
     9181 cggtcgccgg gatcgactga caagcgaatg gcgcgaactg gtcgcttacg tgagtatacg
     9241 ctttgaggcc gggaacagaa ccatatttgg cattgaccat gttcatgact tcgccatgcc
     9301 gcgctgtcgg gaaaaactga ccatcgctcg atgctgacgt gcccatcccc cagacgcgtg
     9361 acatcggcag tttaccctgc gcggccacca caattgccaa tgcctggttc atggcttcgc
     9421 tttcaacatg ccagcgggca aggcgtgaga gctgccagta atcatgcgtg tttgtagctt
     9481 ccgccatctt acgcaggccc agattgagcc cttcagcgag caggacgttg agcagaccga
     9541 tccggtcgcg acatggagcc ccggttctca gatgggtaaa cgcatctgtg aaaccaaggg
     9601 ctgcatcaac ttcaagcagc atgtcggtaa tccgaacgga cggcattcgg cgatacagat
     9661 ccagtatgag tgcctcggca ccatccggca cgtctgctgt caacctgtcg atccgcaacg
     9721 ttccatcttc tatgctaccg tgcggaatag tgccgttacg ggcagcccgg gccagccgct
     9781 taagagcgat cgtgagtcgc gcctttctgt ctgccagcca atcctgtggg ttggaaggca
     9841 cggccagttt tgcattttcc tgcgccgcga tcatcggcac cagtacctgc ttgaggtcac
     9901 catagcggcg cgaatgagcg agccagacat ctccggaacg aaaagcatcc cggaggtgaa
     9961 agagtaccgc cacttcccaa agacgggtat ctcctttttc ctgagctcgt aaatgacggt
    10021 tccatttgga gctgggccgc aggaaacgcc tttctggcga tgcaacacct ttcatctctc
    10081 cgatcgacaa agctgctgct accaatggtc cggcgaccgg cgcggcttcg agcttcagac
    10141 agcgcaacat gcggggcgca taacgacgaa agcgatggta tccctgcccg acatatgcaa
    10201 gaggctcatc ggctagcgtg ttgctgagtt gagtccctgt cgctaccagt tgagcgagcc
    10261 ggtcccatgc aaccgaactg gcgacagcca tctccagcgg ggttccgtca ctgcgggcct
    10321 caagcaacga agctcccagc gcggtgaagg tacggatcgt atccgtgagt gtggctttag
    10381 agccggaaat tgtttcgtca tgctggcgct tcgcttcccg ccaggttttt cctacgatcc
    10441 tgtcatgggt ttcgactatg gcatcagcaa tcgccgcttc ccactccaca acacagacgg
    10501 caaggatcgc ccagcggcgg tccgaagtga tgtcacgcaa accgtcggtg aagtagcgtt
    10561 caccctgccg acgcagccgg gcaatgcgat gggcaggtat gctggccaaa gcactatgat
    10621 tgatattcag ggtacgcaga aattcgagcc tgtcgagcaa acggttagca gcagccgagt
    10681 tgttaccaac ctcgaagttg cgaagccaga tgaaacgact gatattgccg gcgagcattt
    10741 cactcagaag tttgtccagg tgatcgcgaa catccgctgt taaattttcc gcaatccgcg
    10801 tttcaatccg ccgctcagcg gcgaccagag catccgcgca caagcgctcg attgtcgata
    10861 ctgcgggcag aatggtggaa gtttcccgac accgcacaat aaaacgatga gcaagatcct
    10921 cgtttgatct ggcatcttcg gcctggccga aagtccactc ccgcagatca cgggcaccac
    10981 ggcccgtgaa ggtcttgtag ccgtaaattt cgcgcagcgt gtccatgtgc tgctgacggg
    11041 tttggcgccg tgtggcataa gtgagaagcg catcagccgg aactccaagc tgagcaccga
    11101 cgaaggaaag gacttcacgc gggatcatct caccaggagc cagtgcacgg cccggatatc
    11161 gtaaggcaca aagttgcagg gcaaagccaa tcctgttttc cggtctgcgg cgctgcctaa
    11221 tgttttccag gtcatcatcg cccagcgtgt agaacttcag tagcgacagt tcgtccgtgg
    11281 gcagatcgaa cagcgctgct cgctgccgtt cggtgaaaat atggcgtcgt gacatacaaa
    11341 ttcgtccctt ttgaagtata gtctgttttg gacaacagcc agcccatata aatcagggcg
    11401 ttccgataca aaaatccagg agggttcaat tgggacatcg tgccgccatt tactgccggg
    11461 tttcaacagc ggatcagtct tgtgaacgcc aggaatttga tctgcgagcc ttcgccggcc
    11521 gtgccggcta cgacgtggtg ggaatattta aggaaacagg ttcaggaact aaactcgacc
    11581 gggccgagcg aaagaaagtc ctggcgcttg cccagtccag acaaattgat gcaatcctgg
    11641 tcactgagct ttcccggtgg gggcgctcga cgctcgatct gctcaatacg ctacgtgaac
    11701 tggagaactg gaaggtttcc gtgatagcca tgaatggaat ggcgttcgat ctttcgtcgc
    11761 cgtatggacg aatgctggcg acgcttcttt ccggcattgc ggagtttgag cgggatctca
    11821 tcagcgagcg ggtcaagtca ggccttgctg ttgcgaaggc acgtggtaag aggcttggtc
    11881 gtcaggccgg agtgcgacca aaatcagacc gacttttgcc taaggtggtt gcgatgaggg
    11941 ccgagggacg cagctatcgc tggatcgcac gcgagctcgg tatcagcaag aataccgtcg
    12001 ctgacatcgt gcaacgacac agagctaacg cttagggtgt catttcgccc tcagccggaa
    12061 ccgacccct
//
