LOCUS       NZ_CP084839             7566 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB612 plasmid pKp_SB612_9, complete
            sequence.
ACCESSION   NZ_CP084839
VERSION     NZ_CP084839.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024796
            Assembly: GCF_020525965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7566)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7566)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB612
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:31:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 60 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 26 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084839.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7566
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB612"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB612_9"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            join(7443..7566,1..68)
                     /locus_tag="LGM16_RS29065"
                     /old_locus_tag="LGM16_29065"
     CDS             join(7443..7566,1..68)
                     /locus_tag="LGM16_RS29065"
                     /old_locus_tag="LGM16_29065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            68..469
                     /locus_tag="LGM16_RS29070"
                     /old_locus_tag="LGM16_29070"
     CDS             68..469
                     /locus_tag="LGM16_RS29070"
                     /old_locus_tag="LGM16_29070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_060588945.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQSKSEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPAPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1191..4088)
                     /locus_tag="LGM16_RS29075"
                     /old_locus_tag="LGM16_29075"
     CDS             complement(1191..4088)
                     /locus_tag="LGM16_RS29075"
                     /old_locus_tag="LGM16_29075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_000091108.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4183..4788
                     /locus_tag="LGM16_RS29080"
                     /old_locus_tag="LGM16_29080"
     CDS             4183..4788
                     /locus_tag="LGM16_RS29080"
                     /old_locus_tag="LGM16_29080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            5184..5936
                     /locus_tag="LGM16_RS29085"
                     /old_locus_tag="LGM16_29085"
     CDS             5184..5936
                     /locus_tag="LGM16_RS29085"
                     /old_locus_tag="LGM16_29085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001711308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023307481.1"
                     /translation="MGIENSNQGPGWHDHDTPYGTIHSYDGDTGTYGGGGTGGNAGGGH
                     TGLTANTWVDIATTKDYNGRYMSMCLNNLKDHEGSINTMYRDSKGNITVGIGHYLGSAE
                     MAQALPFNRTKVMHVHGDDLESEVAVSKSDIASAFNAYKSNPRDVPSMHLSNADVIGQC
                     IKDVQTTENGLRGLYPGYDRFSDPRKTALVDMGFNLGIPTLKSKFPKFNDAVNRGDWNV
                     AADESHRDLNQRGDKRNKDTADQLRYNR"
                     /name="hypothetical protein"
     gene            6038..6538
                     /locus_tag="LGM16_RS29090"
                     /old_locus_tag="LGM16_29090"
     CDS             6038..6538
                     /locus_tag="LGM16_RS29090"
                     /old_locus_tag="LGM16_29090"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_123829470.1"
                     /translation="MIRFVIAAFISILTIKGAFADINVFIAPNDLNYETNSTSEINHYL
                     DELKKNNTKVDVDKDPKISNELKKYIGIVNDRVSDVFSESEFKKYRGDVCKISITTGNT
                     APSDPVLTQVFTYDELADNKKQSCQKLNNELIKKVAKDKGLSYPKVVKDYGSLSIDMIF
                     LFK"
                     /name="hypothetical protein"
     gene            6586..6720
                     /locus_tag="LGM16_RS29095"
                     /old_locus_tag="LGM16_29095"
     CDS             6586..6720
                     /locus_tag="LGM16_RS29095"
                     /old_locus_tag="LGM16_29095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724569.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_110216848.1"
                     /translation="MSICVIILLAACQANYIRDVKGGTVAPSASSDIRTSSSQPIGSS"
                     /name="colicin release lysis protein"
     gene            complement(6885..7027)
                     /locus_tag="LGM16_RS29295"
                     /pseudo=""
     CDS             complement(6885..7027)
                     /locus_tag="LGM16_RS29295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     Typing          complement(6708..7209)
                     /name="rep_cluster_2358"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="86.415"
                     /coverage="99.0"
                     /evalue="1.02e-154"
ORIGIN
        1 acgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgca ttacaggatg
       61 gtaactgatg gccagagtgt ctattagtga ggctgcccga ctgacgggta agagtagaac
      121 aacactgcac aggttaatta aagcaggtga cctgtccacc tgtagcggtg aacgcaatgc
      181 aaaaatggta gatacttcag agcttttacg tgtctttggg ccctttgaac aatcgaagag
      241 tgaacaccat tctggacagg tctctgaaca gcgtgataca gcctctgctg accagagtga
      301 acaggtgatt cgtcatctga aacaggagat tgaacacctg aacaccttac ttttgtccaa
      361 ggattcgcac attgacagcc tgaaacaggc catgctcctt cttgaaaatc accaggcaaa
      421 attaactgaa tccccggcgc catggtggaa attctggaaa aaatcctgac cccgtcgtgc
      481 ttcagttttc ctctccggcc atcccgggga gttcctgaac cttcccaaaa atcgccagat
      541 ctctgaacgc tcttcaccac taaaaatacc cacacactac gcaaaattaa aattttgcag
      601 atttgtattt aatatcagta gtttactttc atgaaatttc gttttttaaa ttttatttac
      661 atagatccga ctctatgacg actcgtcata gagtccccgg cgagtcgtca tagagtcggt
      721 aacgactcag gactataaaa acaatgagtt aataacgacc cgactctaga cccgactccc
      781 cgtttggttg ttttttagac gcggcaactc tgggtacgac tctagtgagg agtcgccggt
      841 gaatcgtgtg gtgagtcgtc cggcgacttg ccataacgtg attgccggcc gtcgacgaac
      901 ccgattcctg aacgtgctga aaactggttt tccggctgcg ccggcaacgc ttcacggcca
      961 cgcaataaat tgcgtccccg ttcaccgttt tcggatggga agaacgccgg aaacaggact
     1021 taccctggct gaatggcgtg aattttctcg ctatgcactt gcgtgcatac tcatgcatgc
     1081 cttaaaaaca gagcctgcgc gtttctggcg ggttttcggg tggtttgttg cctgttttac
     1141 cggtttcccg tcagaaaggg gtcggttccg gctgagggcg aaatgacacc ctaagctttc
     1201 ggttccttgg gccaaagata ttcgccagtc agtagaatgt gcgcccagcc caatggggat
     1261 atgtggggaa gaaattcagg gggaacatcc aacccttcgt tccgccgctc cgtgacggca
     1321 tgaccaagat ggacggtatt ccagtaaatg atcaccgcag tcaataaatt gagcccagcg
     1381 attcggtagt gctgcccctc tgtcgtgcga tcgcgaattt ccccctgcct cccgatacgg
     1441 agcgcatttt tgagcgcatg gtgggcctct cccttgttaa gaccgatctg agcacgccgc
     1501 tgcatgtctg tatccaggat ccactcaata atgaaaaggg tccgttcaat acgaccaact
     1561 tcacgaagcg caactgcaag gttgttttgt cgtgggtaag aagcgagctt gcgcaggagt
     1621 tggctgggcc tgattttgcc agcggtcatc gtcgcggcac aacggaaaat atcaggccag
     1681 ttcgcaacga taagatcctc ccgggctttt ccacctacca acttgcgtaa ctccctgggg
     1741 gtcgtatcgg gattaaatac gtacaaccgc ttcgatggca gatccctgat tcgcagaacg
     1801 agattgtagc cgagcaggct actggctccg aacaaatggt cggtgaatcc tgctgtatcg
     1861 gcatactgtt cgcgaacatg gcgaccgacc tcgttcatca gtagtccatc gagaatatac
     1921 ggtgcctcgc tcacggtcgc cgggatcgac tgacaagcga atggcgcgaa ctggtcgctt
     1981 acgtgagtat acgctttgag gccgggaaca gaaccatatt tggcattgac catgttcatg
     2041 gcttcgccat gccgcgctgt cgggaaaaac tgaccatcgc tcgatgctga cgtgcccatc
     2101 ccccagacgc gtgacatcgg cagtttaccc tgcgcggcca ccacaattgc caatgcctgg
     2161 ttcatggctt cgctttcaac atgccagcgg gcaaggcgtg agagctgcca gtaatcatgc
     2221 gtgtttgtag cttccgccat cttacgcagg cccagattga gcccttcagc gagcaggacg
     2281 ttgagcagac cgatccggtc gcgacatgga gccccggttc tcagatgggt aaacgcatct
     2341 gtgaaaccaa gggctgcatc aacttcaagc agcatgtcgg taatccgaac ggacggcatt
     2401 cggcgataca gatccagtat gagtgcctcg gcaccatccg gcacgtctgc tgtcaacctg
     2461 tcgatccgca acgttccatc ttctatgcta ccgtgcggaa tagtgccgtt acgggcagcc
     2521 cgggccagcc gcttaagagc gatcgtgagt cgcgcctttc tgtctgccag ccaatcctgt
     2581 gggttggaag gcacggccag ttttgcattt tcctgcgccg cgatcatcgg caccagtacc
     2641 tgcttgaggt caccatagcg gcgcgaatga gcgagccaga catctccgga acgaaaagca
     2701 tcccggaggt gaaagagtac cgccacttcc caaagacggg tatctccttt ttcctgagct
     2761 cgtaaatgac ggttccattt ggagttgggc cgcaggaaac gcctttctgg cgatgcaaca
     2821 cctttcatct ctccgatcga caaagctgct gctaccaatg gtccggcgac cggcgcggct
     2881 tcgagcttca gacagcgcaa catgcggggc gcataacgac gaaagcgatg gtatccctgc
     2941 ccgacatatg caagaggctc atcggctagc gtgttgctga gttgagtccc tgtcgctacc
     3001 agttgagcga gccggtccca tgcaaccgaa ctggcgacag ccatctccag cggggttccg
     3061 tcactgcggg cctcaagcaa cgaagctccc agcgcggtga aggtacggat cgtatccgtg
     3121 agtgtggctt tagagccgga aattgtttcg tcatgctggc gcttcgcttc ccgccaggtt
     3181 tttcctacga tcctgtcatg ggtttcgact atggcatcag caatcgccgc ttcccactcc
     3241 acaacacaga cggcaaggat cgcccagcgg cggtccgaag tgatgtcacg caaaccgtcg
     3301 gtgaagtagc gttcaccctg ccgacgcagc cgggcaatgc gatgggcagg tatgctggcc
     3361 aaagcactat gattgatatt cagggtacgc agaaattcga gcctgtcgag caaacggtta
     3421 gcagcagccg agttgttacc aacctcgaag ttgcgaagcc agatgaaacg actgatattg
     3481 ccggcgagca tttcactcag aagtttgtcc aggtgatcgc gaacatccgc tgttaaattt
     3541 tccgcaatcc gcgtttcaat ccgccgctca gcggcgacca gagcatccgc gcacaagcgc
     3601 tcgattgtcg atactgcggg cagaatggtg gaagtttccc gacaccgcac aataaaacga
     3661 tgagcaagat cctcgtttga tctggcatct tcggcctggc cgaaagtcca ctcacgcaga
     3721 tcacgggcac cacggcccgt gaaggtcttg tagccgtaaa tttcgcgcag cgtgtccatg
     3781 tgctgctgac gggtttggcg ccgtgtggca taagtgagaa gcgcatcagc cggaactcca
     3841 agctgagcac cgacgaagga aaggacttca cgcgggatca tctcaccagg agccagtgca
     3901 cggcccggat atcgtaaggc acaaagttgc agggcaaagc caatcctgtt ttccggtctg
     3961 cggcgctgcc taatgttttc caggtcatca tcgcccagcg tgtagaactt cagtagcgac
     4021 agttcgtccg tgggcagatc gaacagcgct gctcgctgcc gttcggtgaa aatatggcgt
     4081 cgtgacatac aaattcgtcc cttttgaagt atagtctgtt ttggacaaca gccagcccat
     4141 ataaatcagg gcgttccgat acaaaaatcc aggagggttc aattgggaca tcgtgccgcc
     4201 atttactgcc gggtttcaac agcggatcag tcttgtgaac gccaggaatt tgatctgcga
     4261 gccttcgccg gccgtgccgg ctacgacgtg gtgggaatat ttaaggaaac aggttcagga
     4321 actaaactcg accgggccga gcgaaagaaa gtcctggcgc ttgcccagtc cagacaaatt
     4381 gatgcaatcc tggtcactga gctttcccgg tgggggcgct cgacgctcga tctgctcaat
     4441 acgctacgtg aactggagaa ctggaaggtt tccgtgatag ccatgaatgg aatggcgttc
     4501 gatctttcgt cgccgtatgg acgaatgctg gcgacgtttc tttccggcat tgcggagttt
     4561 gagcgggatc tcatcagcga gcgggtcaag tcaggccttg ctgttgcgaa ggcacgtggt
     4621 aagaggcttg gtcgtcaggc cggagtgcga ccaaaatcag accgactttt gcctaaggtg
     4681 gttgctatga gggccgaggg acgcagctat cgctggatcg cacgcgagct cggtatcagc
     4741 aagaataccg tcgctgacat cgtgcaacga cacagagcta acgcttaggg tgtcatttcg
     4801 ccctcagccg gaaccgaccc cagaaacgcc ctgaggccgt tctggcggtg cgtacaatta
     4861 tggctttatg ttaaatcatt gccgcaaaca gccacgaaaa gcttacttgg catcggctct
     4921 actagcctgt taaaactcat attttgaact caatcattta aatttcgtct tttcaggcgt
     4981 acaacttcga taactaaaaa tttatgtgaa atagatattt atccaacagt tatgtagata
     5041 agaataaaac atcaatattt ttttgagagt gtcaaggaag ttatagtgag aatttttcta
     5101 aaagtattga cagattcaaa gaagatgaat aaatttatac tgtttattta tacagttgtt
     5161 tttttgtcaa agaggtaatg tttatgggta ttgaaaattc aaatcagggg ccaggatggc
     5221 atgatcatga tactccttat ggcactattc acagctatga tggtgatact ggtacgtatg
     5281 gaggtggtgg aactggaggg aatgctggtg gtgggcatac cggtcttact gcaaatacat
     5341 gggtagatat cgcgacaaca aaagattata acggtagata tatgtctatg tgtttaaata
     5401 atcttaaaga ccatgaaggt tcaattaata caatgtatag agactctaaa ggtaatatta
     5461 ccgttggtat tggtcattac cttggttctg ctgagatggc tcaggcatta ccttttaatc
     5521 gaacaaaagt tatgcatgtg catggtgatg atcttgaaag tgaggtcgcc gtatctaagt
     5581 ctgatatagc ttcagcattt aatgcataca aatcaaaccc aagagatgtt cctagtatgc
     5641 atctttcaaa tgctgatgta ataggtcagt gtattaagga tgttcaaact actgaaaatg
     5701 gtttgcgtgg actttaccca ggttatgatc gtttttcaga tccacgaaaa acagcattgg
     5761 tagatatggg ttttaatctt ggtattccta cgcttaagtc taaatttccg aagtttaacg
     5821 atgcagttaa tcgtggtgat tggaatgttg ctgcagatga aagtcatcga gatcttaatc
     5881 aaagaggtga taagcgtaat aaagatacgg cagatcaatt gcgttataat cggtaatgat
     5941 ttagatttac aactcctgcc atcaaatggc aggagtgtcc tcacgatatt aaaactaagt
     6001 catgaggttt ctaatcttat attttatggg tggtattatg attcgttttg tgattgctgc
     6061 ttttatttca atcttgacaa ttaaaggagc ttttgctgat atcaatgttt ttatagcgcc
     6121 taacgatcta aattatgaaa caaattctac tagtgaaatt aatcattatt tagatgagct
     6181 gaaaaaaaat aatactaaag ttgatgttga taaagaccca aaaattagca atgagttaaa
     6241 aaaatatatt ggtattgtta acgatagagt gtctgatgtt ttcagtgaaa gtgaatttaa
     6301 gaagtatcgt ggcgatgtct gtaaaatatc aataacaact gggaatacgg ctccatctga
     6361 tcctgttttg actcaagtgt ttacttatga tgaactagct gataataaaa aacaatcatg
     6421 tcaaaaatta aataatgagc taataaaaaa agttgctaaa gataagggtt tatcatatcc
     6481 gaaagttgtt aaagattatg gatctttatc aattgatatg atctttttat ttaaataaaa
     6541 ataaatttac ggtgttgttt atgcctttta aaaaagtctt ttccaatttc tatttgtgta
     6601 attatattgt tggctgcctg ccaagctaac tatattaggg acgtaaaagg aggaactgtt
     6661 gctccatcag cttcttcaga tataagaacg tcgagttcgc aacccatagg atcttcttga
     6721 gatccttagc ggatagtcgt aatctttgct ctgtaaacga aaaaaccacc cggcaaggtg
     6781 gttttttcga aggttcatta accggcaagt ttctgaaccg tggtaactgg ctttcgcaga
     6841 gcacagaaac cgaattagtt ctaacagtgt agctgtagtt tacctcacac ttcaacactt
     6901 ctttgttctg cgaatgcgtt accagtggct gctgccagtg gtgataagtc gtgtcttttc
     6961 gggttggact caagttgata gttaccgaaa aggcgcagca gtcggactga acggggggtt
     7021 cgtgcataca gtccagcttg gagcgaactg cctacccgga actgagtgtc aggcgtggaa
     7081 tgagataaac gcggccataa cagcggaatg acaccggtaa accgaatggc aggaacagga
     7141 gagcgcacga gggagccatc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7201 cgccaccact gatttgagcg tcagattccg tgatgctcgt caggggggcg gagcctatgg
     7261 aaaaacggcc gccttgcggc ctttccttcc tctcctccgt tgaacgctct gctttctcga
     7321 gcttttatac ggatgctcct gccgctcgcc gcagccgaac ggcggaagcg tagcgactga
     7381 gtgagcgagg aagcggaaaa gagaacatcc ctatactgag cactgacgct ctgaggcctc
     7441 ttatgacgaa acaacagaaa acagcgctga acatggctaa attcatccag aatcagtctc
     7501 tgctcttgct ggagaagctt aacgagcttg atctcgatgt tgaggccgac ctttgtgaga
     7561 aactcc
//
