LOCUS       NZ_CP084838             8065 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB612 plasmid pKp_SB612_8, complete
            sequence.
ACCESSION   NZ_CP084838
VERSION     NZ_CP084838.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024796
            Assembly: GCF_020525965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB612
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:31:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 60 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 26 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084838.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB612"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB612_8"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            130..321
                     /locus_tag="LGM16_RS29020"
                     /old_locus_tag="LGM16_29020"
     CDS             130..321
                     /locus_tag="LGM16_RS29020"
                     /old_locus_tag="LGM16_29020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_023302508.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            321..743
                     /locus_tag="LGM16_RS29025"
                     /old_locus_tag="LGM16_29025"
     CDS             321..743
                     /locus_tag="LGM16_RS29025"
                     /old_locus_tag="LGM16_29025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_061500298.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1507..4404)
                     /locus_tag="LGM16_RS29030"
                     /old_locus_tag="LGM16_29030"
     CDS             complement(1507..4404)
                     /locus_tag="LGM16_RS29030"
                     /old_locus_tag="LGM16_29030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_000091108.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            4499..5104
                     /locus_tag="LGM16_RS29035"
                     /old_locus_tag="LGM16_29035"
     CDS             4499..5104
                     /locus_tag="LGM16_RS29035"
                     /old_locus_tag="LGM16_29035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     gene            complement(5255..5371)
                     /locus_tag="LGM16_RS29040"
                     /old_locus_tag="LGM16_29040"
     CDS             complement(5255..5371)
                     /locus_tag="LGM16_RS29040"
                     /old_locus_tag="LGM16_29040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009092571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="WP_102004268.1"
                     /translation="MDGAEAQIAAQAKKAGASYKITESNFNNSVHMTAELTK"
                     /name="YdgH/BhsA/McbA-like domain containing protein"
     gene            complement(5577..6098)
                     /locus_tag="LGM16_RS29045"
                     /old_locus_tag="LGM16_29045"
     CDS             complement(5577..6098)
                     /locus_tag="LGM16_RS29045"
                     /old_locus_tag="LGM16_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_004187661.1"
                     /translation="MTLYYIRRINPLRWNGDLPKDGNYLSLGTDGITNCCKTTDNELSI
                     WLTDSKNPESENNKKVLAVIAAGSEKPSKIDFIFISEDEFNERNLTLKETPGKTAVSSM
                     RYLHRDIENLDVKKLAILGMLIHEKANDDNEIHRVSTSKIKQYVRHAFKDGTPQAEEIK
                     SIDGWSKLYD"
                     /name="hypothetical protein"
     gene            complement(6098..6697)
                     /locus_tag="LGM16_RS29050"
                     /old_locus_tag="LGM16_29050"
     CDS             complement(6098..6697)
                     /locus_tag="LGM16_RS29050"
                     /old_locus_tag="LGM16_29050"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_102004267.1"
                     /translation="MLSFIDYIKKSATPNIEDIQFKGSANLEVPETKVKSDLFNEILKS
                     SKINSSSSNFSYLDDTKLSNYIYNTFLYDDISYESTPKTKYVIENISRELGVQKVDYAL
                     LDILSKKIFKSSDEDIVKFLALLSSFDVKTIPLTSLLSVTSLYSHKSDVVKESVLSTVE
                     YWEWPDIVQYLERMEDFKRPYLQKYKEEVIENLRSL"
                     /name="hypothetical protein"
     gene            complement(6699..7388)
                     /locus_tag="LGM16_RS29055"
                     /old_locus_tag="LGM16_29055"
     CDS             complement(6699..7388)
                     /locus_tag="LGM16_RS29055"
                     /old_locus_tag="LGM16_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004187669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_102004266.1"
                     /translation="MNLVGISYNFNFFTKNNSIKADSESIYKLMPFINDGFMPTTGDEI
                     SEEGDRYKVIRVQKENKDLNISITFSKRAILVQITKDKEIDNVNLMDIVSSTFSKLRET
                     LGDIKGDRTACIANAILENDKETEEKIYKKFFSDEPSYFEWALRKASRFIIEGEESNSV
                     LAVNKGLGTKQNNGTGNITQIEVIVVSCDNNTIAENNTPRFELNNISIFNELLKKTKMD
                     LDKVIGG"
                     /name="hypothetical protein"
     gene            complement(7654..7779)
                     /locus_tag="LGM16_RS29060"
                     /old_locus_tag="LGM16_29060"
     CDS             complement(7654..7779)
                     /locus_tag="LGM16_RS29060"
                     /old_locus_tag="LGM16_29060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001717510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071526724.1"
                     /translation="MHEPPVQSDYHIFSGNYRLESNPERYDKSPVAVAIGKGSTR"
                     /name="replication initiation protein"
     Typing          complement(7671..8065)
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.434"
                     /coverage="96.0"
                     /evalue="2.15e-165"
ORIGIN
        1 ttctcgagct tttacacgca tgctcctgcc gctcgccgca gccgaacggc aggagcatag
       61 cgactgagtg agcgaggaag cggaaaagag aacatcccta cactgagcac tgacgctctg
      121 aggcctctta tgacgaaaca acagaaaaca gcgctgaaca tggctaaatt catccagaat
      181 cagtctctgc tcctgctgga gaagcttaac gagcttgatc tcgatgttga ggcggacctt
      241 tgtgagaaac tccacgatga tgcggagcat cttttccgta cactgtcctc gagactggat
      301 gaattacagg atggtaactg atggccagag tgtctattag tgaagcagca cggcttgtta
      361 aggttagtcg ccctactatt tacaagatga ttaacagtgg taagttaagt tacacctctg
      421 ttgtaaagca cgggaagggt attaaagtta ttgatacatc agagcttatt agagtgtttg
      481 gttctcttga tggtgtcatc gatactgtaa aatatgatgt aaaaagtgac gcagaatcta
      541 caggtattaa cagcgttggt ttacatgatt tacaacatag aatcgcttta cttgaagctg
      601 aaaacgatgg tcttaaaggg gctgttaagg ctcgagatga gcatattgat tcccttcgtc
      661 aggctatgca gcttctggag cacaagcacg aaccttcttc accaccccat tccccgtggt
      721 ggaaattctg gaaaaaatcc tgaccccgtc atgcttctgt tttcctctcc ggccatcccg
      781 gggagttcct gaaccttccc aaaaatcgcc agatctctga acgctcccca cgactaaaaa
      841 tacccacaca ctacgcaaaa ttaaaatttt gcagatttgt atttaatatc agttgtttac
      901 tttcctgaaa ttttgttttt taaattttat ttacatagat ccgactctat gacgactcgt
      961 catagagtcc ccggcgagtc gtcatagagt cggtaacgac tcaggactat aaaaacaata
     1021 agttaataac gacccgactc tagacacgac tccccgtttg gttgtttttt agacgcggcg
     1081 actctgggta cgactctagt gaagagtcgc cggtgaatcg tgtggtgagt cgtccggcga
     1141 cttgccataa cgtgattgcc ggccgtcgac gaacacgatt cctgaacgtg ctgaaaaccg
     1201 gttttccggc tgcgccggca acgcttcacg gccacgcaat aaattgcgtc cccgttcacc
     1261 gttttcggat aggaagaacg ccggaaacag gacttaccct ggctgaatgg cgtgaatttt
     1321 cgcgctatgc acttgcgtgc atactcatgc atgccgtaaa aacagagcct gcgcgtttct
     1381 ggcgggtttt cgggtggttt gttgcctgtt ttaccggttt cccgtcagaa acgccctgag
     1441 gccgttttag cggtgcgtac aatcatgcct ttaggggtcg gttccggctg agggcgaaat
     1501 gacaccctaa gctttcggtt ccttgggcca aagatattcg ccagtcagta gaatgtgcgc
     1561 ccagcccaat ggggatatgt ggggaagaaa ttcaggggga acatccaacc cttcgttccg
     1621 ccgctccgtg acggcatgac caagatggac ggtattccag taaatgatca ccgcagtcaa
     1681 taaattgagc ccagcgattc ggtagtgctg cccctctgtc gtgcgatcgc gaatttcccc
     1741 ctgcctcccg atacggagcg catttttgag cgcatggtgg gcctctccct tgttaagacc
     1801 gatctgagca cgccgctgca tgtctgtatc caggatccac tcaataatga aaagggtccg
     1861 ttcaatacga ccaacttcac gaagcgcaac tgcaaggttg ttttgtcgtg ggtaagaagc
     1921 gagcttgcgc aggagttggc tgggcctgat tttgccagcg gtcatcgtcg cggcacaacg
     1981 gaaaatatca ggccagttcg caacgataag atcctcccgg gcttttccac ctaccaactt
     2041 gcgtaactcc ctgggggtcg tatcgggatt aaatacgtac aaccgcttcg atggcagatc
     2101 cctgattcgc agaacgagat tgtagccgag caggctactg gctccgaaca aatggtcggt
     2161 gaatcctgct gtatcggcat actgttcgcg aacatggcga ccgacctcgt tcatcagtag
     2221 tccatcgaga atatacggtg cctcgctcac ggtcgccggg atcgactgac aagcgaatgg
     2281 cgcgaactgg tcgcttacgt gagtatacgc tttgaggccg ggaacagaac catatttggc
     2341 attgaccatg ttcatggctt cgccatgccg cgctgtcggg aaaaactgac catcgctcga
     2401 tgctgacgtg cccatccccc agacgcgtga catcggcagt ttaccctgcg cggccaccac
     2461 aattgccaat gcctggttca tggcttcgct ttcaacatgc cagcgggcaa ggcgtgagag
     2521 ctgccagtaa tcatgcgtgt ttgtagcttc cgccatctta cgcaggccca gattgagccc
     2581 ttcagcgagc aggacgttga gcagaccgat ccggtcgcga catggagccc cggttctcag
     2641 atgggtaaac gcatctgtga aaccaagggc tgcatcaact tcaagcagca tgtcggtaat
     2701 ccgaacggac ggcattcggc gatacagatc cagtatgagt gcctcggcac catccggcac
     2761 gtctgctgtc aacctgtcga tccgcaacgt tccatcttct atgctaccgt gcggaatagt
     2821 gccgttacgg gcagcccggg ccagccgctt aagagcgatc gtgagtcgcg cctttctgtc
     2881 tgccagccaa tcctgtgggt tggaaggcac ggccagtttt gcattttcct gcgccgcgat
     2941 catcggcacc agtacctgct tgaggtcacc atagcggcgc gaatgagcga gccagacatc
     3001 tccggaacga aaagcatccc ggaggtgaaa gagtaccgcc acttcccaaa gacgggtatc
     3061 tcctttttcc tgagctcgta aatgacggtt ccatttggag ttgggccgca ggaaacgcct
     3121 ttctggcgat gcaacacctt tcatctctcc gatcgacaaa gctgctgcta ccaatggtcc
     3181 ggcgaccggc gcggcttcga gcttcagaca gcgcaacatg cggggcgcat aacgacgaaa
     3241 gcgatggtat ccctgcccga catatgcaag aggctcatcg gctagcgtgt tgctgagttg
     3301 agtccctgtc gctaccagtt gagcgagccg gtcccatgca accgaactgg cgacagccat
     3361 ctccagcggg gttccgtcac tgcgggcctc aagcaacgaa gctcccagcg cggtgaaggt
     3421 acggatcgta tccgtgagtg tggctttaga gccggaaatt gtttcgtcat gctggcgctt
     3481 cgcttcccgc caggtttttc ctacgatcct gtcatgggtt tcgactatgg catcagcaat
     3541 cgccgcttcc cactccacaa cacagacggc aaggatcgcc cagcggcggt ccgaagtgat
     3601 gtcacgcaaa ccgtcggtga agtagcgttc accctgccga cgcagccggg caatgcgatg
     3661 ggcaggtatg ctggccaaag cactatgatt gatattcagg gtacgcagaa attcgagcct
     3721 gtcgagcaaa cggttagcag cagccgagtt gttaccaacc tcgaagttgc gaagccagat
     3781 gaaacgactg atattgccgg cgagcatttc actcagaagt ttgtccaggt gatcgcgaac
     3841 atccgctgtt aaattttccg caatccgcgt ttcaatccgc cgctcagcgg cgaccagagc
     3901 atccgcgcac aagcgctcga ttgtcgatac tgcgggcaga atggtggaag tttcccgaca
     3961 ccgcacaata aaacgatgag caagatcctc gtttgatctg gcatcttcgg cctggccgaa
     4021 agtccactca cgcagatcac gggcaccacg gcccgtgaag gtcttgtagc cgtaaatttc
     4081 gcgcagcgtg tccatgtgct gctgacgggt ttggcgccgt gtggcataag tgagaagcgc
     4141 atcagccgga actccaagct gagcaccgac gaaggaaagg acttcacgcg ggatcatctc
     4201 accaggagcc agtgcacggc ccggatatcg taaggcacaa agttgcaggg caaagccaat
     4261 cctgttttcc ggtctgcggc gctgcctaat gttttccagg tcatcatcgc ccagcgtgta
     4321 gaacttcagt agcgacagtt cgtccgtggg cagatcgaac agcgctgctc gctgccgttc
     4381 ggtgaaaata tggcgtcgtg acatacaaat tcgtcccttt tgaagtatag tctgttttgg
     4441 acaacagcca gcccatataa atcagggcgt tccgatacaa aaatccagga gggttcaatt
     4501 gggacatcgt gccgccattt actgccgggt ttcaacagcg gatcagtctt gtgaacgcca
     4561 ggaatttgat ctgcgagcct tcgccggccg tgccggctac gacgtggtgg gaatatttaa
     4621 ggaaacaggt tcaggaacta aactcgaccg ggccgagcga aagaaagtcc tggcgcttgc
     4681 ccagtccaga caaattgatg caatcctggt cactgagctt tcccggtggg ggcgctcgac
     4741 gctcgatctg ctcaatacgc tacgtgaact ggagaactgg aaggtttccg tgatagccat
     4801 gaatggaatg gcgttcgatc tttcgtcgcc gtatggacga atgctggcga cgtttctttc
     4861 cggcattgcg gagtttgagc gggatctcat cagcgagcgg gtcaagtcag gccttgctgt
     4921 tgcgaaggca cgtggtaaga ggcttggtcg tcaggccgga gtgcgaccaa aatcagaccg
     4981 acttttgcct aaggtggttg ctatgagggc cgagggacgc agctatcgct ggatcgcacg
     5041 cgagctcggt atcagcaaga ataccgtcgc tgacatcgtg caacgacaca gagctaacgc
     5101 ttagggtgtc atttcgccct cagccggaac cgaccccctt tatgttaaat aaccgatgcc
     5161 aaaaagcccc cgaaggggcc tgaaggtttt gctgctaatg gacgaaacga gccgcctccc
     5221 ggcgacctgt tcggcagcta actctgcgac catcttattt ggttagctct gcggtcatgt
     5281 gaacgctgtt gttaaagttg gattcagtaa ttttgtaaga cgcaccggct tttttcgcct
     5341 gagcggcgat ctgggcctct gcgccgtcaa tggtggatgt actgagatgt tatggtaaat
     5401 tatgctgaca attctaatag gtagcattca atatcgagtt aataaaatca aattgaagtt
     5461 gattttatta gtagataaga agggtttgat ttttactcag tgagtaaaaa tcttttttga
     5521 agataaaaag ctcgcatatg cgagcttttt atttatagga gagtgatctg tccttctcag
     5581 tcatataatt ttgaccaacc atcaatgctt tttatctctt cagcctgtgg ggtgccatct
     5641 ttaaaagcat gcctaacata ttgttttatt ttagatgtgc taactctgtg aatttcatta
     5701 tcatcgttag ctttttcatg gataagcatt cctagaatag ctagcttttt cacatctaag
     5761 ttctcaatgt ccctgtgaag gtaacgcatt gatgaaacag cagttttacc gggtgtttct
     5821 tttagggtta gattccgttc attaaattca tcttcgctta tgaaaataaa atcaattttt
     5881 gatggttttt cagatcctgc agcaatcaca gctaagactt ttttgttgtt ttcgctttct
     5941 gggtttttac tgtcagttaa ccagatagat agttcattgt cagtggtttt acagcagttc
     6001 gtaatcccat ctgtacctag agatagataa tttccgtctt ttggtagatc accattccac
     6061 cgcaaagggt ttatgcgcct aatgtaatac agggtcatta taaactcctt aaattctcaa
     6121 tgacctcttc tttgtatttc tgcaagtaag gtcttttaaa atcttccatt ctttctagat
     6181 actgtactat gtctggccac tcccagtatt ccacagtgga taatacactt tctttcacaa
     6241 catcactctt gtgcgagtac aagctagtca cggaaagcaa tgaagttaat ggtatagttt
     6301 taacatcaaa agaggataat aaagccaaaa acttcacgat atcctcatca gaagatttga
     6361 atatcttttt gcttagtata tcaaggagag cataatctac tttttgcact cctaactccc
     6421 tagagatatt ctctatcaca tactttgttt ttggtgtaga ttcatagctt atatcgtcat
     6481 acaaaaaagt attgtatata taattagaaa gctttgtgtc atctaagtat gagaaattag
     6541 atgaactact atttattttt gatgatttta aaatctcatt aaaaagatca gacttaacct
     6601 ttgtctcggg tacttcaaga ttggcgcttc ctttgaattg aatgtcttca atgttaggtg
     6661 tagcgctttt ttttatataa tctataaagg aaagcatatt aacctccaat gactttatct
     6721 aaatccattt tcgttttctt tagtaactca ttgaaaatgc taatattatt caattcaaat
     6781 ctaggtgtat tgttttcggc aatggtatta ttgtcacaag ataccacaat gacttctatt
     6841 tgagtaatat tacctgtccc attgttttgt ttagttccca gtcctttatt tacagctaaa
     6901 acgctgttgg attcctcacc ttcaataatg aatctactcg cttttcttag cgcccattca
     6961 aaataagatg gctcatcaga aaagaatttt ttatatattt tttcttctgt ttctttatcg
     7021 ttttcaagta ttgcattcgc aatgcaggca gtacgatcgc ctttaatgtc tccgagcgtc
     7081 tctctcagct ttgaaaatgt ggaactaact atgtccatta aatttacgtt gtctatctct
     7141 ttatcttttg ttatctgtac taaaattgct cttttagaga atgtaatgga tatgtttaaa
     7201 tctttatttt ctttttgtac gcgaattact ttatatctat caccctcctc acttatttca
     7261 tcgccagtcg tcggcataaa gccatcattg ataaaaggca tgagtttata tattgactca
     7321 ctatctgcct ttattgagtt atttttagtg aagaagttaa aattatacga aatcccgact
     7381 aaattcatct ttgaatccct catttagatg attgcattaa acaacttata agtaaatttt
     7441 agaagtggaa agtgatttct tgacgaaaat tatgtgtgat cgtatctttg cagcgtaaac
     7501 aaaaaaacca cctggggagg tggttttttc gaaggttcaa caggttgggg aacttgttga
     7561 accgtggtaa cagggtgtgc aagaccgctg caaccaaatt cgtccttcca gtttagccgt
     7621 agttatgctg aaacttcaag aactctgcat cagttatctt gtactccctt taccaatggc
     7681 taccgccact ggcgatttgt cgtatctttc cgggttggac tcaagacgat agttaccgga
     7741 aaagatgtgg tagtcggact gaacgggggg ttcgtgcata cagtccagct tggagcgaac
     7801 tgcctacccg gaactgagtg tcaggcgtgg aatgagataa acgcggccat aacagcggaa
     7861 tgacaccggt aaaccgaatg gcaggaacag gagagcgcgc gagggagcca tcagggggaa
     7921 acgcctgata tctttatagt cctgtcgggt ttcgccacca ctgatttgag cgtcagcttc
     7981 tgtgatgctt gtcagggggg cggagcctat ggaaaaacgg ccgctaagcg gcctttcctt
     8041 ttccccgtca cgcgaccact cggtt
//
