LOCUS       NZ_CP084837             8210 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB612 plasmid pKp_SB612_7, complete
            sequence.
ACCESSION   NZ_CP084837
VERSION     NZ_CP084837.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024796
            Assembly: GCF_020525965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB612
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:31:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 60 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 26 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084837.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB612"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB612_7"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            join(7138..8210,1..1825)
                     /locus_tag="LGM16_RS28970"
                     /old_locus_tag="LGM16_28970"
     CDS             join(7138..8210,1..1825)
                     /locus_tag="LGM16_RS28970"
                     /old_locus_tag="LGM16_28970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020326821.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn5403 family transposase"
                     /protein_id="WP_000091108.1"
                     /translation="MSRRHIFTERQRAALFDLPTDELSLLKFYTLGDDDLENIRQRRRP
                     ENRIGFALQLCALRYPGRALAPGEMIPREVLSFVGAQLGVPADALLTYATRRQTRQQHM
                     DTLREIYGYKTFTGRGARDLREWTFGQAEDARSNEDLAHRFIVRCRETSTILPAVSTIE
                     RLCADALVAAERRIETRIAENLTADVRDHLDKLLSEMLAGNISRFIWLRNFEVGNNSAA
                     ANRLLDRLEFLRTLNINHSALASIPAHRIARLRRQGERYFTDGLRDITSDRRWAILAVC
                     VVEWEAAIADAIVETHDRIVGKTWREAKRQHDETISGSKATLTDTIRTFTALGASLLEA
                     RSDGTPLEMAVASSVAWDRLAQLVATGTQLSNTLADEPLAYVGQGYHRFRRYAPRMLRC
                     LKLEAAPVAGPLVAAALSIGEMKGVASPERRFLRPNSKWNRHLRAQEKGDTRLWEVAVL
                     FHLRDAFRSGDVWLAHSRRYGDLKQVLVPMIAAQENAKLAVPSNPQDWLADRKARLTIA
                     LKRLARAARNGTIPHGSIEDGTLRIDRLTADVPDGAEALILDLYRRMPSVRITDMLLEV
                     DAALGFTDAFTHLRTGAPCRDRIGLLNVLLAEGLNLGLRKMAEATNTHDYWQLSRLARW
                     HVESEAMNQALAIVVAAQGKLPMSRVWGMGTSASSDGQFFPTARHGEAMNMVNAKYGSV
                     PGLKAYTHVSDQFAPFACQSIPATVSEAPYILDGLLMNEVGRHVREQYADTAGFTDHLF
                     GASSLLGYNLVLRIRDLPSKRLYVFNPDTTPRELRKLVGGKAREDLIVANWPDIFRCAA
                     TMTAGKIRPSQLLRKLASYPRQNNLAVALREVGRIERTLFIIEWILDTDMQRRAQIGLN
                     KGEAHHALKNALRIGRQGEIRDRTTEGQHYRIAGLNLLTAVIIYWNTVHLGHAVTERRN
                     EGLDVPPEFLPHISPLGWAHILLTGEYLWPKEPKA"
                     /name="Tn3-like element Tn5403 family transposase"
     gene            <1858..2529
                     /locus_tag="LGM16_RS28975"
                     /old_locus_tag="LGM16_28975"
                     /pseudo=""
     CDS             <1858..2529
                     /locus_tag="LGM16_RS28975"
                     /old_locus_tag="LGM16_28975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611473.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /name="IS3 family transposase"
     gene            complement(2899..3011)
                     /locus_tag="LGM16_RS28980"
                     /old_locus_tag="LGM16_28980"
                     /pseudo=""
     CDS             complement(2899..3011)
                     /locus_tag="LGM16_RS28980"
                     /old_locus_tag="LGM16_28980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            3427..3618
                     /locus_tag="LGM16_RS28985"
                     /old_locus_tag="LGM16_28985"
     CDS             3427..3618
                     /locus_tag="LGM16_RS28985"
                     /old_locus_tag="LGM16_28985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_004187666.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDVEADLCEKLHDDA
                     EHLFRTLSSRLDALQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            3618..4019
                     /locus_tag="LGM16_RS28990"
                     /old_locus_tag="LGM16_28990"
     CDS             3618..4019
                     /locus_tag="LGM16_RS28990"
                     /old_locus_tag="LGM16_28990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048333944.1"
                     /translation="MARVSISEAARLTGKSRTTLHRLIKAGDLSTCSGERNAKMVDTSE
                     LLRVFGPFEQSKSEHHSGQVSEQRDTASADQSEQVIRHLKQEIEHLNTLLLSKDSHIDS
                     LKQAMLLLENHQAKLTESPTPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(5075..5395)
                     /locus_tag="LGM16_RS29000"
                     /old_locus_tag="LGM16_29000"
     CDS             complement(5075..5395)
                     /locus_tag="LGM16_RS29000"
                     /old_locus_tag="LGM16_29000"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_202963179.1"
                     /translation="MMRVLSLGAILMAMSALPLTAISQVTLAPDGSYVSGSEATLAPDG
                     SYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEVTLAPDGSYVGGSEATLAPDGSYV
                     GN"
                     /name="hypothetical protein"
     gene            complement(5527..5718)
                     /locus_tag="LGM16_RS29005"
                     /old_locus_tag="LGM16_29005"
     CDS             complement(5527..5718)
                     /locus_tag="LGM16_RS29005"
                     /old_locus_tag="LGM16_29005"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_049106033.1"
                     /translation="MLKKDDIIFMKSCGNAKTIIFLRGVDEKLMINASFEEIKFAINNN
                     LQELPGVQAVAATLKIAT"
                     /name="hypothetical protein"
     gene            5960..>6410
                     /locus_tag="LGM16_RS29010"
                     /old_locus_tag="LGM16_29010"
                     /pseudo=""
     CDS             5960..>6410
                     /locus_tag="LGM16_RS29010"
                     /old_locus_tag="LGM16_29010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611478.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0004803 - transposase activity [Evidence IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /name="transposase"
     gene            complement(6438..7043)
                     /locus_tag="LGM16_RS29015"
                     /old_locus_tag="LGM16_29015"
     CDS             complement(6438..7043)
                     /locus_tag="LGM16_RS29015"
                     /old_locus_tag="LGM16_29015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_000509966.1"
                     /translation="MGHRAAIYCRVSTADQSCERQEFDLRAFAGRAGYDVVGIFKETGS
                     GTKLDRAERKKVLALAQSRQIDAILVTELSRWGRSTLDLLNTLRELENWKVSVIAMNGM
                     AFDLSSPYGRMLATFLSGIAEFERDLISERVKSGLAVAKARGKRLGRQAGVRPKSDRLL
                     PKVVAMRAEGRSYRWIARELGISKNTVADIVQRHRANA"
                     /name="recombinase family protein"
     Typing          complement(2891..3269)
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.211"
                     /coverage="88.0"
                     /evalue="1.77e-156"
ORIGIN
        1 ccggctcgct caactggtag cgacagggac tcaactcagc aacacgctag ccgatgagcc
       61 tcttgcatat gtcgggcagg gataccatcg ctttcgtcgt tatgcgcccc gcatgttgcg
      121 ctgtctgaag ctcgaagccg cgccggtcgc cggaccattg gtagcagcag ctttgtcgat
      181 cggagagatg aaaggtgttg catcgccaga aaggcgtttc ctgcggccca actccaaatg
      241 gaaccgtcat ttacgagctc aggaaaaagg agatacccgt ctttgggaag tggcggtact
      301 ctttcacctc cgggatgctt ttcgttccgg agatgtctgg ctcgctcatt cgcgccgcta
      361 tggtgacctc aagcaggtac tggtgccgat gatcgcggcg caggaaaatg caaaactggc
      421 cgtgccttcc aacccacagg attggctggc agacagaaag gcgcgactca cgatcgctct
      481 taagcggctg gcccgggctg cccgtaacgg cactattccg cacggtagca tagaagatgg
      541 aacgttgcgg atcgacaggt tgacagcaga cgtgccggat ggtgccgagg cactcatact
      601 ggatctgtat cgccgaatgc cgtccgttcg gattaccgac atgctgcttg aagttgatgc
      661 agcccttggt ttcacagatg cgtttaccca tctgagaacc ggggctccat gtcgcgaccg
      721 gatcggtctg ctcaacgtcc tgctcgctga agggctcaat ctgggcctgc gtaagatggc
      781 ggaagctaca aacacgcatg attactggca gctctcacgc cttgcccgct ggcatgttga
      841 aagcgaagcc atgaaccagg cattggcaat tgtggtggcc gcgcagggta aactgccgat
      901 gtcacgcgtc tgggggatgg gcacgtcagc atcgagcgat ggtcagtttt tcccgacagc
      961 gcggcatggc gaagccatga acatggtcaa tgccaaatat ggttctgttc ccggcctcaa
     1021 agcgtatact cacgtaagcg accagttcgc gccattcgct tgtcagtcga tcccggcgac
     1081 cgtgagcgag gcaccgtata ttctcgatgg actactgatg aacgaggtcg gtcgccatgt
     1141 tcgcgaacag tatgccgata cagcaggatt caccgaccat ttgttcggag ccagtagcct
     1201 gctcggctac aatctcgttc tgcgaatcag ggatctgcca tcgaagcggt tgtacgtatt
     1261 taatcccgat acgaccccca gggagttacg caagttggta ggtggaaaag cccgggagga
     1321 tcttatcgtt gcgaactggc ctgatatttt ccgttgtgcc gcgacgatga ccgctggcaa
     1381 aatcaggccc agccaactcc tgcgcaagct cgcttcttac ccacgacaaa acaaccttgc
     1441 agttgcgctt cgtgaagttg gtcgtattga acggaccctt ttcattattg agtggatcct
     1501 ggatacagac atgcagcggc gtgctcagat cggtcttaac aagggagagg cccaccatgc
     1561 gctcaaaaat gcgctccgta tcgggaggca gggggaaatt cgcgatcgca cgacagaggg
     1621 gcagcactac cgaatcgctg ggctcaattt attgactgcg gtgatcattt actggaatac
     1681 cgtccatctt ggtcatgccg tcacggagcg gcggaacgaa gggttggatg ttccccctga
     1741 atttcttccc cacatatccc cattgggctg ggcgcacatt ctactgactg gcgaatatct
     1801 ttggcccaag gaaccgaaag cttagggtgt catttcgccc tcagccggaa ccgaccccac
     1861 cgacgcatct ggcagttact gcgccgtgaa ggccttcatg ttaatcacaa gcgcgtgtac
     1921 cgtctttacc accttaacgg gctgggtgta aaacgcagac gacgtcgtaa aggtctggca
     1981 acagaacgtc tgccgctgct ccgcccggag gcgcccaacc tgacctggcc gatggatttt
     2041 gtcatggacg cgctggccac cggtcgcagg atcaagtgcc tgacctgcgt ggacgacttc
     2101 acgaaggagt gtctgacaat taccgccgtc ttcgggattt caggcgttca ggtcacgcga
     2161 attctggaca gcattgcact gtttcgcggc tatccggcga cgataagaac tgaccagggg
     2221 ccggagttta cctgcagagc actcgaccag tgggcttatg agcatggggt ggagctacgg
     2281 cttatccagc cgggcaggcc aacacagaac ggatttattg aaagttttaa cggacgattc
     2341 agggatgagt gcctcaatga gcactggttc agcgatatcg ttcacgccag gaaaacgatt
     2401 aatgactggc ggcaggatta gagcgagtgt cgtccacatt catcgctgaa ctaccagact
     2461 ccggctgaat ttgcggcggg ctggcgaaac gggaaatatg aagaaaaacg aaccgacatt
     2521 actaactgaa ggttgtatct aatcctgggg gggcaggtca accgcgttgg atgattgtgg
     2581 atcatgatta tatatgtttt tttcttatca gaaacaatgg gttaaatagt tttattttga
     2641 tggttggtgt cgcggagtgg tgctggtttc acttcagggg taggcatatg gaacaggatc
     2701 ctcttgagat cctttcttta gcatcgtaat ctcttgctca gtaaacgaaa aaaccgccca
     2761 gcaaggcggt tttctcgaag gttctcagag cagcaactct ttgaaccgag gtaactggct
     2821 taaagggagc gcagtcacca aaactgttcg tttagtttag ccctaactgg cgtataactt
     2881 caagactaac tccctaaatc agttaccagt ggctgctgcc agtggtgata agtcgtgtct
     2941 tttcgggttg gactcaagtt gatagttacc gaaaaggcgc agcagtcgga ctgaacgggg
     3001 ggttcgtgca tacagtccag cttggagcga actgcctacc cggaactgag tgtcaggcgt
     3061 ggaatgagat aaacgcggcc ataacagcgg aatgacaccg gtaaaccgaa tggcaggaac
     3121 aggagagcgc acgagggagc catcaggggg aaacgcctgg tatctttata gtcctgtcgg
     3181 gtttcgccac cactgatttg agcgtcagat tccgtgatgc tcgtcagggg ggcggagcct
     3241 atggaaaaac ggccgccttg cggcctttcc ttcctctcct ccgttgaacg ctctgctttc
     3301 tcgagctttt atacggatgc tcctgccgct cgccgcagcc gaacggcgga agcgtagcga
     3361 ctgagtgagc gaggaagcgg aaaagagaac atccctatac tgagcactga cgctctgagg
     3421 cctcttatga cgaaacaaca gaaaacagcg ctgaacatgg ctaaattcat ccagaatcag
     3481 tctctgctct tgctggagaa gcttaacgag cttgatctcg atgttgaggc cgacctttgt
     3541 gagaaactcc acgatgatgc ggagcatctt ttccgtacac tgtcctcgag actggatgca
     3601 ttacaggatg gtaactgatg gccagagtgt ctattagtga ggctgcccga ctgacgggta
     3661 agagtagaac aacactgcac aggttaatta aagcaggtga cctgtccacc tgtagcggtg
     3721 aacgcaatgc aaaaatggta gatacttcag agcttttacg tgtctttggg ccctttgaac
     3781 aatcgaagag tgaacaccat tctggacagg tctctgaaca gcgtgataca gcctctgctg
     3841 accagagtga acaggtgatt cgtcatctga aacaggagat tgaacacctg aacaccttac
     3901 ttttgtccaa ggattcgcac attgacagcc tgaaacaggc catgctcctt cttgaaaatc
     3961 accaggcaaa attaactgaa tccccgacgc catggtggaa attctggaaa aaatcctgac
     4021 cccgtcatgc ttcagttttc ctctccggcc atcccgggga gttcctgaac cttcccaaaa
     4081 atcgtcagat ctctgaacgc tcctcaccac taaaaatacc cacaccccac gcaaaattaa
     4141 aattttgcag attccttatt tacaacaatg ggttaatgaa aaataaatgt gcggtgatgt
     4201 gttacaaagt gcagatccaa gtcgtggaca actctatggc gaatcgctga cgaattgccc
     4261 acgattcata tgtgaatcac gctttattat catattgtta tgatgaatcg actcttgatt
     4321 caattcatca ctctgggtgt tttttgttca tggtgactct tgattcaatt cacgattcga
     4381 atcgccggtg aatcgtgtgg taagtcgtcc ggcgacttgc cataacgtga ttgccggccg
     4441 tcgacgaacc cgattcctga acgtgctgaa aaccggtttt ccggctgcgc cggcaacgct
     4501 tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggataggaa gaacgccgga
     4561 aacaggactt accctggctg aatggcgtga attttcgcgc tatgcacttg cgtgcatact
     4621 catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt ggtttgttgc
     4681 ctgttttacc ggtttcccgt cagaaacgcc ttgaggccgt ttttgcggtg cgtacaagaa
     4741 gacgttatgt taaatagggc gctatcgcct aaatgcacga ctgattgatt ttttttctct
     4801 gaagtatgag attcctgagt agacagctac aacaagtaga gcaatggcga tagcaagtaa
     4861 aacaaagtcc ataagtacct gattaattta ataaaaaacc tgttatgatt agttattgta
     4921 agtggttttc attctttttt cattacactt attgctttga gctaatggtt cctgaggacg
     4981 cattgttcag tacacgtaag aagacgttat gataaatcca aaaatgctgg cattcggaat
     5041 tcagtatatc ggtacgcagc ataataatgg ggggttaatt tccaacgtat gagccatcag
     5101 gtgctaaggt tgcttccgaa ccaccaacgt atgagccatc aggtgctaag gttacttccg
     5161 aaccaccaac gtatgagcca tcaggtgcta aggttacttc cgaaccacca acgtatgagc
     5221 catcaggtgc taaggttact tccgaaccac caacgtatga gccatcaggt gctaaggttg
     5281 cttccgaacc actaacgtac gatccatcag gtgctaaggt tacttgggat atcgcggtta
     5341 gtggtaatgc tgacatagcc attaaaatgg caccaagaga taatactctc atcactatta
     5401 ctcctgttaa tatcaaaaat agaattgaat cgttcagcgg tgccgaacgc cggttccagc
     5461 cgcgggattc gtgatcggga tgttatagta aattgatctc ttgattttga gtgcccttac
     5521 tcgaaatcag gttgctattt tcagtgtcgc ggctactgct tgtacccccg gaagctcctg
     5581 aagattatta ttgatagcaa actttatctc ttcaaagctc gcgttaatca tgagcttctc
     5641 atccacgcca cgtaaaaata ttattgtttt tgcattacca catgatttca taaagataat
     5701 atcatctttt tttagcatga tactgtcgtt acttagtgta gtgagataaa gcatagccac
     5761 tgtttttgtg tgtttttgtt aaggctgtta agattattcc taaccactga ttttgtacat
     5821 atcatcaaaa aaaataaaaa aactaatgcc cccgtggtgg gtatataaca aatcacgggg
     5881 agcggagctg acctgctccc cgttgattaa tacaccgcga tgttagtaat gtcttcataa
     5941 gccacatgag gacatcctca tgaagaagcg tttttccgac gaacagatca tcagtattct
     6001 ccgcgaggcc gaagccgggg tttctgcccg tgagctctgc cgtaagcacg ccatttccga
     6061 cgccatcttt tacacctggc gtaagaagta tggcggtatg gaggtgcccg aggttaagcg
     6121 ccttaagtcg cttgaggaag agaacgccag actcaagaaa ctgcttgccg aagccatgct
     6181 ggataagcag gcgcttcagg tggctctggg gcgaaagtac tgacgacaga ccagaagcag
     6241 gaagccgttg agtttatgtg tgatgcgacc ggtctgtcgc aacgtagtgc ctgcaggctt
     6301 acaggtttgc ccctgtcgac ctgccgctat gaggctcagc gttcggcggc tgatgcgcat
     6361 ttatcactga gctggcactg gagcgcaggc gttttggcta ccgaggggtc ggttccggct
     6421 gagggcgaaa tgacacccta agcgttagct ctgtgtcgtt gcacgatgtc agcgacggta
     6481 ttcttgctga taccgagctc gcgtgcgatc cagcgatagc tgcgtccctc ggccctcata
     6541 gcaaccacct taggcaaaag tcggtctgat tttggtcgca ctccggcctg acgaccaagc
     6601 ctcttaccac gtgccttcgc aacagcaagg cctgacttga cccgctcgct gatgagatcc
     6661 cgctcaaact ccgcaatgcc ggaaagaaac gtcgccagca ttcgtccata cggcgacgaa
     6721 agatcgaacg ccattccatt catggctatc acggaaacct tccagttctc cagttcacgt
     6781 agcgtattga gcagatcgag cgtcgagcgc ccccaccggg aaagctcagt gaccaggatt
     6841 gcatcaattt gtctggactg ggcaagcgcc aggactttct ttcgctcggc ccggtcgagt
     6901 ttagttcctg aacctgtttc cttaaatatt cccaccacgt cgtagccggc acggccggcg
     6961 aaggctcgca gatcaaattc ctggcgttca caagactgat ccgctgttga aacccggcag
     7021 taaatggcgg cacgatgtcc caattgaacc ctcctggatt tttgtatcgg aacgccctga
     7081 tttatatggg ctggctgttg tccaaaacag actatacttc aaaagggacg aatttgtatg
     7141 tcacgacgcc atattttcac cgaacggcag cgagcagcgc tgttcgatct gcccacggac
     7201 gaactgtcgc tactgaagtt ctacacgctg ggcgatgatg acctggaaaa cattaggcag
     7261 cgccgcagac cggaaaacag gattggcttt gccctgcaac tttgtgcctt acgatatccg
     7321 ggccgtgcac tggctcctgg tgagatgatc ccgcgtgaag tcctttcctt cgtcggtgct
     7381 cagcttggag ttccggctga tgcgcttctc acttatgcca cacggcgcca aacccgtcag
     7441 cagcacatgg acacgctgcg cgaaatttac ggctacaaga ccttcacggg ccgtggtgcc
     7501 cgtgatctgc gtgagtggac tttcggccag gccgaagatg ccagatcaaa cgaggatctt
     7561 gctcatcgtt ttattgtgcg gtgtcgggaa acttccacca ttctgcccgc agtatcgaca
     7621 atcgagcgct tgtgcgcgga tgctctggtc gccgctgagc ggcggattga aacgcggatt
     7681 gcggaaaatt taacagcgga tgttcgcgat cacctggaca aacttctgag tgaaatgctc
     7741 gccggcaata tcagtcgttt catctggctt cgcaacttcg aggttggtaa caactcggct
     7801 gctgctaacc gtttgctcga caggctcgaa tttctgcgta ccctgaatat caatcatagt
     7861 gctttggcca gcatacctgc ccatcgcatt gcccggctgc gtcggcaggg tgaacgctac
     7921 ttcaccgacg gtttgcgtga catcacttcg gaccgccgct gggcgatcct tgccgtctgt
     7981 gttgtggagt gggaagcggc gattgctgat gccatagtcg aaacccatga caggatcgta
     8041 ggaaaaacct ggcgggaagc gaagcgccag catgacgaaa caatttccgg ctctaaagcc
     8101 acactcacgg atacgatccg taccttcacc gcgctgggag cttcgttgct tgaggcccgc
     8161 agtgacggaa ccccgctgga gatggctgtc gccagttcgg ttgcatggga
//
