LOCUS       NZ_CP084836             8638 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB612 plasmid pKp_SB612_6, complete
            sequence.
ACCESSION   NZ_CP084836
VERSION     NZ_CP084836.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024796
            Assembly: GCF_020525965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8638)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB612
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:31:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 60 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 26 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084836.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB612"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB612_6"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            complement(744..1166)
                     /locus_tag="LGM16_RS28920"
                     /old_locus_tag="LGM16_28920"
     CDS             complement(744..1166)
                     /locus_tag="LGM16_RS28920"
                     /old_locus_tag="LGM16_28920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_061500298.1"
                     /translation="MARVSISEAARLVKVSRPTIYKMINSGKLSYTSVVKHGKGIKVID
                     TSELIRVFGSLDGVIDTVKYDVKSDAESTGINSVGLHDLQHRIALLEAENDGLKGAVKA
                     RDEHIDSLRQAMQLLEHKHEPSSPPHSPWWKFWKKS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(1166..1357)
                     /locus_tag="LGM16_RS28925"
                     /old_locus_tag="LGM16_28925"
     CDS             complement(1166..1357)
                     /locus_tag="LGM16_RS28925"
                     /old_locus_tag="LGM16_28925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_032421723.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSSRLDELQDGN"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(1828..2007)
                     /locus_tag="LGM16_RS28930"
                     /old_locus_tag="LGM16_28930"
     CDS             complement(1828..2007)
                     /locus_tag="LGM16_RS28930"
                     /old_locus_tag="LGM16_28930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_136048841.1"
                     /translation="MNKQQKTAVNMAKFIQDQSLLLLDMDLDHEADFCEKLHEDAERLY
                     RSLSARLNEQQDGA"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            2426..2569
                     /locus_tag="LGM16_RS29290"
     CDS             2426..2569
                     /locus_tag="LGM16_RS29290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_074192474.1"
                     /translation="MHEPPVQSDRCAFSGNYHLESNPERHDKTPLAAATGNGFAEQRSV
                     EV"
                     /name="replication initiation protein"
     gene            complement(2772..3469)
                     /locus_tag="LGM16_RS28935"
                     /old_locus_tag="LGM16_28935"
     CDS             complement(join(2772..3220,3220..3469))
                     /locus_tag="LGM16_RS28935"
                     /old_locus_tag="LGM16_28935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_136049344.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPEPVTSRIQPGSDVIV
                     CAEMDEQWGYIGAKSRQRWLFYAYDRIRRTIVVHVFGERALATLERLLELLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERNNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            3674..4780
                     /locus_tag="LGM16_RS28940"
                     /old_locus_tag="LGM16_28940"
     CDS             3674..4780
                     /locus_tag="LGM16_RS28940"
                     /old_locus_tag="LGM16_28940"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013036402.1"
                     /GO_function="GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="WP_136049343.1"
                     /translation="MNTQTFRGVQALRGIAALSVMLFHFRWNINAQIPGRGDALFGWGA
                     TGVDLFFLISGFVITLSAAKAPQGISGALNFLKKRALRILPAYYIILVISFFLSGAMST
                     FHYADKTENFISALTFMPILPDHAPFYVDDGGMFGVRWTLNYEVMFYLFISVALLFAKR
                     WVWAGVLFAATLVVLPLTLGYQLTLQPAGYKVDSALLGLITNPMTWLFLTGMLIGLALP
                     YLRWLSSRVMAVAAVCSLIAAAYFFSHGMYAGHGLLSSGWVYGLILLTFALSEDVIGKY
                     VPNFLIRLGDISFSLYLIHTLMNNGLGKRFESFGIEDGCVRFVVSIALSCLLAWLSWRY
                     IERPFIRVGKIKTVSKEKKCCSIFSSKP"
                     /name="acyltransferase"
     gene            4874..5571
                     /locus_tag="LGM16_RS28945"
                     /old_locus_tag="LGM16_28945"
     CDS             join(4874..5123,5123..5571)
                     /locus_tag="LGM16_RS28945"
                     /old_locus_tag="LGM16_28945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558270.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_136049344.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTILRHFKKLRPEPVTSRIQPGSDVIV
                     CAEMDEQWGYIGAKSRQRWLFYAYDRIRRTIVVHVFGERALATLERLLELLSAFEVVVW
                     MTDGWPLYESRLKGKLHVISKRYTQRIERNNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     gene            5833..6264
                     /locus_tag="LGM16_RS28950"
                     /old_locus_tag="LGM16_28950"
     CDS             5833..6264
                     /locus_tag="LGM16_RS28950"
                     /old_locus_tag="LGM16_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6530 family protein"
                     /protein_id="WP_094308951.1"
                     /translation="MNIPIHLKHKPIIAVENYDRIDGPYADETDAMGLSVGIAQWNTPG
                     YTELSAKVWRNTGEKWSRQSEELPLHRVIDLATLICIAMDYSKHGRLSSSDDFPVSRSG
                     GNPELSRHIDLMKKELKENHKHLDESLGRLMAELKRLGY"
                     /name="DUF6530 family protein"
     gene            6558..7418
                     /locus_tag="LGM16_RS28955"
                     /old_locus_tag="LGM16_28955"
     CDS             6558..7418
                     /locus_tag="LGM16_RS28955"
                     /old_locus_tag="LGM16_28955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013510118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_094308949.1"
                     /translation="MNKLIELVKKRHKEDKSHIELTYVTHFYCNQKNINSITDLLKKYE
                     TYSRNLIEKIEFIIVDDCSPLEYEIPDLNLNYRWLKITTDIQWNQGGARNLGVTYAKSD
                     KIIMSDLDHELPEHTLDYLVKHRNPGRKFYKLHRKHNDGSFYKGHSNLFFMSRSRFLRF
                     HGYDEEFCGHYGAEDYRFVKYHKYHGSKQGYLPKKIWCSKRQLDFKNSNHSLSRDLSYN
                     TPVDIRKKEECENFGLEYGHSRLFLNFDWITLSKKNIRPDEIPVIRRYWRPLWWFRWLI
                     PFLSR"
                     /name="glycosyltransferase family A protein"
     gene            complement(7799..8269)
                     /locus_tag="LGM16_RS28960"
                     /old_locus_tag="LGM16_28960"
     CDS             complement(7799..8269)
                     /locus_tag="LGM16_RS28960"
                     /old_locus_tag="LGM16_28960"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136048842.1"
                     /translation="MRMKYFLLATVLASASAIASPETDLKHCVAEANRNSNVNAARDCF
                     ITFTNRFSANTPIANGIVLRNFDHDDGYIIYNFQLTNVDYNTGSEQSLQLLEEIRNWLD
                     SEDATKSQRKGYCKTLGKARDVFEDGAIMRFYDKKGLFITLKVIEASECESH"
                     /name="hypothetical protein"
     gene            <8310..8582
                     /locus_tag="LGM16_RS28965"
                     /old_locus_tag="LGM16_28965"
                     /pseudo=""
     CDS             <8310..8582
                     /locus_tag="LGM16_RS28965"
                     /old_locus_tag="LGM16_28965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001676030.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /name="PH domain-containing protein"
     Typing          2187..2534
                     /name="rep_cluster_2373"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="94.556"
                     /coverage="81.0"
                     /evalue="6.43e-151"
ORIGIN
        1 agctggattt accataacat ccgttatcag caccgcctca gaggccctca gggcgtttct
       61 gacgggaaac cggtaaaaca ggcaacaaac cacccgaaaa cccgccagaa acgcgcagac
      121 tctgttttta cggcatgcat gagtatgcac gcaagtgcat agcgcgaaaa ttcacgccat
      181 tcagccgggg taagtcctgt ttccgatgtt cttcttaccc gaaaacggtg aacggggacg
      241 caatttattg cgtggccgtg aagcgttgcc ggcgcagccg gaaaacccgc tgtcagcacg
      301 ttcaggaatc gggttcgtcg acggccggca atcacgttat ggcaagtcgc cggacgactc
      361 accacacgat tcaccggcga ttcgactcgt gacttgactc tagagtcacc acggtcataa
      421 aaacgccaaa gcgacgattc gactctagag tcaagtcgtc ataagttact gatagtgtgg
      481 agtgagtcgt cgatgactct atgacgagtc gtcggcaagt cgtacacgaa tcaccgacga
      541 cttggattga ccttacagaa ggtaatactg cgtaatttaa taaaacctaa ccaactgata
      601 ataaggagtt atctgcaaaa ttttaatttt gcgtggtgtg tgggtatttt tcgtggtgag
      661 gagtgttccc ggatctggcg atttttggga aggttcagga acgccccggg atggccggag
      721 aggaaaactc aagcatggcg ggatcaggat tttttccaga atttccacca cggggaatgg
      781 ggtggtgaag aaggttcgtg cttgtgctcc agaagctgca tagcctgacg aagggaatca
      841 atatgctcat ctcgagcctt aacagcccct ttaagaccat cgttttcagc ttcaagtaaa
      901 gcgattctat gttgtaaatc atgtaaacca acgctgttaa tacctgtaga ttctgcgtca
      961 ctttttacat catattttac agtatcgatg acaccatcaa gagaaccaaa cactctaata
     1021 agctctgatg tatcaataac tttaataccc ttcccgtgct ttacaacaga ggtgtaactt
     1081 aacttaccac tgttaatcat cttgtaaata gtagggcgac taaccttaac aagccgtgct
     1141 gcttcactaa tagacactct ggccatcagt taccatcctg taattcatcc agtctcgagg
     1201 acagtgtacg gaaaagatgc tccgcatcat cgtggagttt ctcacaaagg tcggcctcag
     1261 catcgagatc aagctcgtta agcttctcca gcaggagcag agactgattc tggatgaatt
     1321 tagccatgtt cagcgctgtt ttctgttgtt tcgtcataag ggacctcaga gcatcaatgc
     1381 tcagtgtaaa accggtaagg gggtcggatg tacgaatggt tcagagaacg gatctgaggt
     1441 aatcctgtag ttattttgta gtttatctgt agtcatcgca ctaaaaatca ggattaagtc
     1501 atcagctttt gtagtctttc tgtagtttac ctgtagtccg gaaaaataat ccgttcatgc
     1561 gagcatgaac gaggtcgtta atgacgtttt tttgtcaccg acgcccctta acctgcgtaa
     1621 atcacccgcc atggttgatt atttttcaac ctcaaaatgg tcatacacct aacgcagccg
     1681 cgattttcat cgcaacttca tgaaaaataa ataaaaaaga aaaaatacag ggattctggg
     1741 tattaccggg gtaataatgt taagcgctaa cacaatgaaa attaaggcga tgccggtatt
     1801 actccggtaa tacccagaac ggagccatta cgcaccgtcc tgctgctcat tcaggcgcgc
     1861 tgacaggctt cggtaaaggc gttccgcatc ctcatggagt ttctcgcaga aatcagcctc
     1921 atgatccaga tccatgtcca gaagaagcag ggactgatcc tggatgaatt tagccatgtt
     1981 aacggcagtc ttttgttgtt tgttcatgaa ggccccggcg cgaaagtgct tactgtacgt
     2041 tctgatggtt ttccgcttcc tcgctcattc gctcactccg ttcggtcatg agtctgcggc
     2101 gagcggaggg aagataagca gataacgtaa cggtaagcat aacgaaagct aaggacaagg
     2161 gcgaaaaggc cacgccccgc cggggcgttt ttccataggc tccgcccccc tgacgagtat
     2221 cataaaatct gacgctcaaa tcagaggtgg cgaaacccga caggactata aagatcccag
     2281 gtgtttcccc ctggtagctc cctcgcgcgc tctcctgttc ctgccattcg gtttaccggt
     2341 gtcattccgc tgttatggcc gcgtttatct cattccacgc ctgacactca gttccgggaa
     2401 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
     2461 cttctccggt aactatcatc ttgagtccaa cccggagagg cacgacaaaa cgccactggc
     2521 agcagccact ggtaacggat tcgcagaaca aagaagtgtt gaagtgtgag gtgaagtaca
     2581 gctacactgt aataactaat ttggtttctg tgctctgcga aagccagtta cctcggttaa
     2641 aaatctgcca ggatttttaa ccttcgaaaa accacctgcc agggtggttt tttcgtctag
     2701 tgggaaggag attacgacgg gttagagatg atctcaagag gatccgctaa aaatcgggta
     2761 atgactccaa cttactgata gtgttttatg ttcagataat gcccgatgac cttgtcatgc
     2821 agctccaccg attttgagaa cgacagtgac ttccgtccca gccttgccag atgttgtctc
     2881 agattcaggt tatttcgctc aatgcgctgc gtatatcgct tgctgataac gtgcagcttt
     2941 cccttcaggc gggattcata cagcggccag ccatccgtca tccataccac gacctcaaag
     3001 gccgacagca gctccagaag acgctccagt gtggccagag cgcgttcacc gaagacgtgc
     3061 accacaatcg tcctccgtat cctgtcatac gcgtaaaaca gccagcgctg acgagattta
     3121 gccccgatgt agccccactg ttcgtccatt tcagcgcaga caatcacatc actgcccggt
     3181 tgtatgcgcg atgttaccgg ctccggcctg agttttttaa gtgtcgtaaa atcgtgttga
     3241 ggccaacgcc cataatgcgg gcggttgccc ggcatccaac accattcatg gccatatcaa
     3301 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgtagc tgccatgttt
     3361 tacggcagtg agagcagaga tagcgctgat gtccggcagt acttttaccg ttacgcacca
     3421 cgccttcagt agcggagcag gaaggacatc tgatggaaat ggaagccacg caagcacctt
     3481 aaaatcacca tcatacacta aatcagtaag ttggcagcat tacccagcca ctaacagttt
     3541 ccatatcgat acgccgaata accaaaatca cacatcatgt tttgttgctg ttgcagtctg
     3601 tgcaagacag taaattaacc tgtaagattc ctgcctttct gtttacacaa cactgcgatt
     3661 aagcttaaac aatatgaaca ctcagacttt tagaggcgta caggcgctga gaggaattgc
     3721 tgcgctaagc gttatgcttt tccacttccg atggaacata aatgctcaaa tcccgggacg
     3781 cggagatgcg ctttttggat ggggagcaac gggtgtcgat ctcttctttc ttatcagcgg
     3841 attcgttata acgctgagcg ccgcaaaagc acctcagggt attagcggtg cccttaattt
     3901 tttaaagaaa agggcgctga gaatattgcc ggcttactac atcattctgg tcatttcttt
     3961 cttcctgtcc ggtgcgatga gcacgtttca ttatgcagat aaaacggaaa attttatcag
     4021 cgcattgacc tttatgccga tactccccga tcatgcgcct ttttatgttg atgatggcgg
     4081 aatgtttggc gtcagatgga cgctgaatta tgaggttatg ttttacctgt ttatatccgt
     4141 cgctcttctg tttgctaagc gctgggtatg ggctggcgtt ctgtttgcgg caacgcttgt
     4201 tgtgctgcct ttaactttgg gttatcagct aactcttcag cctgccggtt ataaggttga
     4261 ttcagccctg ctgggattaa tcactaaccc aatgacttgg ttgttcctta ccggaatgtt
     4321 gattggactg gcgcttcctt atctgagatg gctatcatcc agggttatgg cagttgctgc
     4381 agtatgtagc ctgattgcag cagcctactt ctttagtcat ggcatgtatg ccgggcatgg
     4441 gctcctcagt tcgggctggg tatatggact tattctatta acctttgctc tttctgaaga
     4501 tgtcatcggg aaatatgtgc caaacttttt aattcggtta ggcgatattt ctttctctct
     4561 ttatttaatc cataccttga tgaataatgg attaggaaag cgattcgaaa gttttgggat
     4621 tgaagacgga tgtgtgaggt ttgtagtttc cattgctctt tcatgtttgc ttgcctggct
     4681 ctcatggcgc tatattgaaa ggccgtttat tcgggtaggt aaaattaaaa ctgtcagtaa
     4741 ggaaaaaaaa tgctgcagta tcttctccag caagccataa ataataaata gcagcacctg
     4801 attttcattc tctcagccgg taatgctgcc aacttactga tttagtgtat gatggtgatt
     4861 ttaaggtgct tgcgtggctt ccatttccat cagatgtcct tcctgctccg ctactgaagg
     4921 cgtggtgcgt aacggtaaaa gtactgccgg acatcagcgc tatctctgct ctcactgccg
     4981 taaaacatgg cagctacagt tcacttacac cgcttctcaa cccggtacgc accagaaaat
     5041 cattgatatg gccatgaatg gtgttggatg ccgggcaacc gcccgcatta tgggcgttgg
     5101 cctcaacacg attttacgac acttaaaaaa ctcaggccgg agccggtaac atcgcgcata
     5161 caaccgggca gtgatgtgat tgtctgcgct gaaatggacg aacagtgggg ctacatcggg
     5221 gctaaatctc gtcagcgctg gctgttttac gcgtatgaca ggatacggag gacgattgtg
     5281 gtgcacgtct tcggtgaacg cgctctggcc acactggagc gtcttctgga gctgctgtcg
     5341 gcctttgagg tcgtggtatg gatgacggat ggctggccgc tgtatgaatc ccgcctgaag
     5401 ggaaagctgc acgttatcag caagcgatat acgcagcgca ttgagcgaaa taacctgaat
     5461 ctgagacaac atctggcaag gctgggacgg aagtcactgt cgttctcaaa atcggtggaa
     5521 ctgcatgaca aagtcatcgg acactatctg aacataaaac actatcagta agttggagtc
     5581 atcacccact aaagccagtt acccggttaa gcagttcccc aactgaccta accttcgatc
     5641 aaaccacctc cccaggtggt tttttcgttt tcagggcaag agattacgct cagccataaa
     5701 ttatctcaag aagatccatt ccaatagaac caaattcact cagaaacaaa atgtttcatt
     5761 tagtagttag taaattacca tattctgttg ttttatgagt catgaaacta tctttacaca
     5821 gttgagagca atatgaacat ccctattcat ctgaaacata aaccaattat tgcagtagag
     5881 aactatgacc gaattgacgg accctacgct gatgaaactg atgctatggg gctttccgtt
     5941 gggattgctc aatggaatac tcccggatat acagagctat cagcgaaggt ctggcgcaat
     6001 accggagaaa aatggtcacg acaatctgaa gaactccccc ttcaccgagt catagactta
     6061 gcaacgctta tctgcatagc aatggattac agtaaacatg gtcgcttatc ttctagtgat
     6121 gatttcccag tcagccgctc aggaggtaat cctgagttgt cacgccacat tgatttgatg
     6181 aaaaaagaac taaaagaaaa tcacaagcat ctagatgaga gtctaggacg cctaatggca
     6241 gaactaaaac gcttgggtta ttaagattca aacggacggc aagagataac ttccaaccta
     6301 aaaaggcaac ttgtaggtaa cggcatcaga taagcaacat tttttatatt aaaaacacta
     6361 cgggtaaggg tcttcaataa tgaagctagc aaaaaagcat tgtatgagat gtggtaaaaa
     6421 aagaagtctt ctatcaagag tcatgagttt agatcatgac ttttgtgact cgtgtttatt
     6481 catcatcaat gatgaaataa gaaacaaaag aacaaagaaa taatttaatt gagtatttaa
     6541 aattggtaac cgaacacatg aacaagttaa ttgaattagt aaaaaaaaga cataaggaag
     6601 acaaatctca tattgagcta acctatgtca ctcattttta ttgcaaccaa aaaaacatca
     6661 attctataac ggatttatta aaaaagtacg agacgtactc acgtaactta atagaaaaaa
     6721 ttgaattcat tattgttgac gactgctcac ctttagaata tgaaatacca gaccttaacc
     6781 tcaattacag atggttgaag ataaccactg acatacaatg gaaccaaggg ggagcaagaa
     6841 acctcggagt cacttatgcc aaatcagata aaatcataat gtctgattta gatcatgaat
     6901 taccggagca tacattagat tacttagtaa agcacagaaa tcccggacgt aaattttata
     6961 agctacacag aaaacataat gatggttctt tttacaaagg gcactccaat ttatttttca
     7021 tgagccgctc tcgctttctt agatttcatg gctacgacga ggaattttgt ggtcattacg
     7081 gggctgaaga ttatcgtttt gttaaatatc ataaatacca tggttcaaag caaggctact
     7141 tacctaaaaa aatatggtgt tcgaaacgac aacttgactt caaaaatagc aatcatagcc
     7201 tctcaaggga tttgtcatat aacaccccag tagatataag aaaaaaagaa gagtgtgaaa
     7261 actttgggct agaatacgga catagtcgct tatttttaaa tttcgactgg atcacgctat
     7321 caaagaaaaa tatcaggcca gatgaaatcc cagttatacg cagatattgg agaccgttat
     7381 ggtggtttag atggctaata ccattcttgt cgcgataaca tgttttacat ttaccataac
     7441 atccgttatc agcaccgcct gagaggcttt caggggtcag tttggatata gagaattatt
     7501 gtacggtaag cctgtttttt gaacagactt acctatagca atagggtgta ctcccgcgct
     7561 atacggcatt ttcagaggtt tgaccttaat cggataacct aaatgcgata ctgcaagagc
     7621 gacaacttaa aagcgatagc cactgacgca atatcaaccc cttacgaacc aagattggtt
     7681 aaataatgcg cttaacgtac aaaaatttcc gatctccaaa ctgaccccaa gcctatctct
     7741 tatctggaga tacagctaca gtccggtgtt tcagggggct acatcactac ccgtttgtct
     7801 agtgtgattc acactcactt gcctcaatta cttttaaagt gatgaataat ccttttttat
     7861 cgtaaaaacg cattattgca ccatcctcaa aaacatctct agctttccca agtgttttgc
     7921 aataaccctt tctttgtgat ttggtcgcat cttcagaatc aagccaattt cttatctctt
     7981 ccaagagctg taatgattgt tccgacccag tattgtagtc cacgttagtc aactggaaat
     8041 tgtagatgat gtagccatcg tcatggtcaa agttgcgtaa gacgatacca ttagctattg
     8101 gtgtgttggc ggaaaaacga tttgtgaatg tgataaagca atctcttgct gcattgacat
     8161 ttgagtttct attggcctct gccacacaat gcttgagatc tgtttcgggt gaagcaatag
     8221 cactggccga tgctaatact gttgctagta aaaaatactt cattctcatt ctaagccccc
     8281 ttctaactat catagtgctg tgacctgccc ttagagtttg gtcaacggag cttgcattaa
     8341 ctaaccaaag agtcatcgca aaagtgggtt tgatccgacg caatacggtg gaactccgta
     8401 ttgataaagt tgaaagcctt ggcatacatc aagggatact gggacgaatc tttaactatg
     8461 ggtctatcgt gattaaaggc actggcggaa ccaacacccc aattccaaac atcaaggcgc
     8521 caatgcaatt cagatcgatt gtgaacaatc atatcgagga aatgaattct aagcagcagt
     8581 agcttcctct tagcattaca aaaaaagccc tacggggctt tttttgtacc tgccaatc
//
