LOCUS       NZ_CP084835             9989 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain SB612 plasmid pKp_SB612_5, complete
            sequence.
ACCESSION   NZ_CP084835
VERSION     NZ_CP084835.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024796
            Assembly: GCF_020525965.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9989)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9989)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name                     :: Klebsiella pneumoniae SB612
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:31:38
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,833
            CDSs (total)                      :: 5,708
            Genes (coding)                    :: 5,547
            CDSs (with protein)               :: 5,547
            Genes (RNA)                       :: 125
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 11
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 60 of 161
            Pseudo Genes (incomplete)         :: 100 of 161
            Pseudo Genes (internal stop)      :: 26 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084835.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9989
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB612"
                     /host="Water"
                     /db_xref="taxon:573"
                     /plasmid="pKp_SB612_5"
                     /geo_loc_name="Netherlands"
                     /collection_date="2000"
     gene            join(9055..9989,1)
                     /locus_tag="LGM16_RS28855"
                     /old_locus_tag="LGM16_28855"
     CDS             join(9055..9989,1)
                     /locus_tag="LGM16_RS28855"
                     /old_locus_tag="LGM16_28855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="WP_226618087.1"
                     /translation="MMLLSIIIPCYNCESNIEKLLLSIKEQLNETVQLVLINDGSWDAT
                     HDIITKFTSDNPSIKIKYINSINQGAAKARTTGLNYAEGDYVFFCDSDDMISANFISVF
                     TELSNDKPDMIYFCSTVLDSQGNRFKKVHFPLAYIESNSNEVFNYLLKKGQWTAAVWTY
                     IFRKDLVLKSGAIFTDRKVHEDHLFTLRLLAGSEKISICNSELYFQNVTSGSLTKSPKK
                     IDYLTERYKSYKEAREDIIYKFSQESIVLYDRWSMSSLLQILKENKKMVFQGIMHTAIY
                     RVLWKEKLVILDFVKSKVKKKITRSKSLSQ"
                     /name="glycosyltransferase family 2 protein"
     gene            223..906
                     /locus_tag="LGM16_RS28860"
                     /old_locus_tag="LGM16_28860"
                     /pseudo=""
     CDS             223..906
                     /locus_tag="LGM16_RS28860"
                     /old_locus_tag="LGM16_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            1447..1668
                     /locus_tag="LGM16_RS28865"
                     /old_locus_tag="LGM16_28865"
     CDS             1447..1668
                     /locus_tag="LGM16_RS28865"
                     /old_locus_tag="LGM16_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016162091.1"
                     /translation="MEFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            1661..2548
                     /locus_tag="LGM16_RS28870"
                     /old_locus_tag="LGM16_28870"
     CDS             1661..2548
                     /locus_tag="LGM16_RS28870"
                     /old_locus_tag="LGM16_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_094333876.1"
                     /translation="MAKFASAARQAAGVIKTLQGGILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            2545..3243
                     /locus_tag="LGM16_RS28875"
                     /old_locus_tag="LGM16_28875"
     CDS             2545..3243
                     /locus_tag="LGM16_RS28875"
                     /old_locus_tag="LGM16_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(3250..3648)
                     /locus_tag="LGM16_RS28880"
                     /old_locus_tag="LGM16_28880"
     CDS             complement(3250..3648)
                     /locus_tag="LGM16_RS28880"
                     /old_locus_tag="LGM16_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_048290302.1"
                     /translation="MATVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFASVPVEQRGEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRPWWRFWQS"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(3648..3839)
                     /locus_tag="LGM16_RS28885"
                     /old_locus_tag="LGM16_28885"
     CDS             complement(3648..3839)
                     /locus_tag="LGM16_RS28885"
                     /old_locus_tag="LGM16_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4251..4382
                     /locus_tag="LGM16_RS28890"
                     /old_locus_tag="LGM16_28890"
     CDS             4251..4382
                     /locus_tag="LGM16_RS28890"
                     /old_locus_tag="LGM16_28890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_094333875.1"
                     /translation="MHEPPVQSDCCALSGNYRLESNPVRHAKAPLAAATGKQDVREM"
                     /name="replication initiation protein"
     gene            4691..5068
                     /locus_tag="LGM16_RS28895"
                     /old_locus_tag="LGM16_28895"
     CDS             4691..5068
                     /locus_tag="LGM16_RS28895"
                     /old_locus_tag="LGM16_28895"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142368761.1"
                     /translation="MIHNHPTWVIKSMIYRLGILSISLLTVGCVSTSNVLELGRDTYTV
                     SATADGFRDAASARQSAFKTGSEKCNSLGKKFLLTNENVANTRMGIDTTVTLYFRCLDE
                     RDSEYVRPNLQKAPDLLIESK"
                     /name="hypothetical protein"
     gene            5125..5430
                     /locus_tag="LGM16_RS28900"
                     /old_locus_tag="LGM16_28900"
     CDS             5125..5430
                     /locus_tag="LGM16_RS28900"
                     /old_locus_tag="LGM16_28900"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136049327.1"
                     /translation="MMKVGNVREYGPTKSHLLLMVTTLPAGVGVFIYPIVEVIWGELSL
                     LKVAGYTFLSLIVGGLLANIIVRGLSATTFGILLVNPLAMICSIAGLIVLAIQHLP"
                     /name="hypothetical protein"
     gene            complement(5618..6301)
                     /locus_tag="LGM16_RS28905"
                     /old_locus_tag="LGM16_28905"
                     /pseudo=""
     CDS             complement(5618..6301)
                     /locus_tag="LGM16_RS28905"
                     /old_locus_tag="LGM16_28905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     gene            6483..7310
                     /locus_tag="LGM16_RS28910"
                     /old_locus_tag="LGM16_28910"
     CDS             6483..7310
                     /locus_tag="LGM16_RS28910"
                     /old_locus_tag="LGM16_28910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004198917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="WP_136048846.1"
                     /translation="MISVNLTTMHSRLDLCAATVWSIINQSVQPDKINLWISNEPYLSD
                     HGICEIPAWVEELNKINNIVNVHYVKNTGPYRKIINAIINSEIDDILVYADDDVIYGQQ
                     WLEFLLGQFYENNCNVAIAARIRIMERNIFNKYKSYSSYSISMKKDKFEKDFIITGVGG
                     CVLSKRMIKPSIIGDDAYLTISPKTDDIWISKMLEASNTKVESSPDALRCVHEILHGYN
                     TLSSINTLTSKNSSLIIKAIRMVKNKFYSYFGLQKTNNDKSIKMVNLHFNGKL"
                     /name="glycosyltransferase family A protein"
     gene            7592..9058
                     /locus_tag="LGM16_RS28915"
                     /old_locus_tag="LGM16_28915"
     CDS             7592..9058
                     /locus_tag="LGM16_RS28915"
                     /old_locus_tag="LGM16_28915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017803471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOP flippase family protein"
                     /protein_id="WP_168443553.1"
                     /translation="MNLKSKTIHAVKWNLLSTVCVTGLGMVSLWILSSLFTTTDYGIIS
                     AALILSTVFSIITDFGISNSVIRSEKLNKYELSSLYIINVFLGMIFCLGLFIFSAQLAS
                     LFNGGTELAKQIKIMSFSLIISSFGGQPRALLARELRFDVISRITILSAIFNFVFAVFL
                     SYVYRSPWCVAVSLLVSSSISSVLYIYSSKGLRDIKLQFFTLKEIKKHVSYGSQLVMDS
                     IINQISVNTYPVLMARLISLTAIGGYNISYNISVALFEKLNPVLSHALFPAFSKIQNDA
                     VSLKKTFLKVTMFSSLVNFPMLLGMMLISDYVVTVFFEQKWQFITPIVQILCLVGAARS
                     LDVPVISVLLAKGQMYRNVYLGVFKLALGIPLTWFLGKQYGLTGIVFSFLCIQIVNLVM
                     GYFFQVRPCLNIKVKSYIYAVFVPLIHTLPMLVTGYLLILFLTITSPILSMVVIIFSCM
                     SVYIASILTSPVKEIKEFNAIVLNNILRNK"
                     /name="MOP flippase family protein"
     Typing          3888..4360
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="91.423"
                     /coverage="91.0"
                     /evalue="0.0"
ORIGIN
        1 gggattatcg tataatgggt aatgatgcca atttgttgat ttcgcatttt cactgacgcc
       61 aggatcacct gatgttacaa ggattgaatg gctacagatt caaatggtca ccaacaggtt
      121 ggcagcatta ctatttgccg ttataggcta attttatggt ctttctgggt gatgctgcca
      181 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
      241 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag cactgccgga
      301 catcagcgct atctctgctc tcactgccgt aaaacatggc agctacagtt cacttacacc
      361 gcctctcagc ccggtacgca ccagaaaatc attgatatgg ccatgaatgg cgttggatgc
      421 cgggcaaccg cccgcattat gggcgttggc ctcaacacga ttttacgtca cttaaaaaac
      481 tcaggccgca gtcggtaacc tcgcgcatac agccgggcag tgacgtcatc gtctgcgcgg
      541 aaatggacga acagtggggc tatgtcgggg ctaaatcgcg ccagcgctgg ctgttggctc
      601 cggaagacgg ttgttgcgca cgtattcggt gaacgcacta tggcgacgct ggggcgtctt
      661 atgagcctgc tgtcaccctt tgacgtggtg atatggatga cggatggctg gccgctgtat
      721 gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc gatatacgca gcgaattgag
      781 cggcataacc tgaatctgag gcagcacctg gcacggctgg gacggaagtc gctgtcgttc
      841 tcaaaatcgg tggagctgca tgacaaggtc atcgggcatt atctgaacat aaaacactat
      901 caataatttt gagtcattac caatttcatc ctgtccctcc gttttctctg tgacgtcatt
      961 ctggcatcac gacggtcacc gttggcgggg tccggggcgg tcagccccgg cgttctttat
     1021 tcaggcaata tgagcacctg cgacacccgc tatgtacgac gcataaatgc gcctgtacat
     1081 agcggttgtc gctattgagc ggagtcagac gcaacattta aaaaacaaca ttcacacgca
     1141 cgaaaaacct ggctagtatt tagtcaaacc atgagcccgc gcactctgac gcgcacctgc
     1201 ctaccggcag caggtaaaac agccgggtcc ctccggggat actgtaccgg gcagcccaat
     1261 ccgagaacac tgagacaaca gcccaaatct gtcagaccca caaccgggtc aagtcattct
     1321 ctgactaaag agacgttgag ccgcgagcag tccgggctta accacgttac ggtttaaacg
     1381 agggcgatcg gcgcaggccg cagtggattt ccactggcgt tcttacaact caacaccgga
     1441 gttcctgtgg aatttgatac cgccgccgaa ctggccgccc tgcaggcgca gacccgccgt
     1501 atccgccagg tccgctaccg tccctcccgc cttgaccgct ataccggcga actgctgagc
     1561 ctgtaccagg ccggggccag tgcagcggag ctgcaacgct ggctccgggc ccggagaatc
     1621 aaagtggtgt tgtccacggt tacccgctgg ctggaaaaaa atggctaaat tcgccagcgc
     1681 tgcccgccag gcggctggcg tgataaaaac cctccagggc ggtattttgc gctctgtcgg
     1741 cacggtccgg aattatgagc agggactgac ccgcgtcgca gagtggatga aagccaaccg
     1801 gatcaaccac ggtgatttac gcacactgac accggcgcag gccatcacct acctggagca
     1861 gcgcggggag caggtcggcc agaaaacgct cgatatggaa cgccaggcca tccaggccat
     1921 gatgatccac gtgacgcaga ccctccctgc cggcgaacac ctgccggtgg tgaaatcgga
     1981 gcaggcacag atcctgagta gccgggccta cagcccggac caggtagccc tgattgccgc
     2041 agcacaacag cccgccaacg ctctggccac cgcgattgct ctggccgccg gattacgcgc
     2101 ccatgaactg ctgaccctcg cgccccgcca ggagcgagca gcggatcccc gtccggcagc
     2161 ggacagtaaa tttgccggtc gggagggtgt gatttacacc gttcagggca aaggcggcct
     2221 ggtcagggaa gttctgctgc cgtcacgcct ggctgaacgt ctcgagcatg tcaggctggc
     2281 gagcccggtc cgggtgaccg atcgcggcgt gttttaccag agtcagtacg ccattggcgg
     2341 aggccagcgc tggtcaaacg ccttcagtgc cgcctccatg cgtaccctgg gctggtcacg
     2401 gggggcacac ggcgtgcgtc acagctacgc ccagcaacgg atgcaggagc tgcaaaaact
     2461 cggtctggtt cgtgatatgg ccctgcgtac cgtgagccag gaaatgggcc attttcgtcc
     2521 ggaaatcacg gagacatacc tgcgatgaca agaacattta ttttattgtg taccggtgtt
     2581 ttcgccatcg cgtacgccct gtatgagctg aatcactatg gcgtttacta tccggacgtc
     2641 agcaatcctg aggcgtttac tggctggaag gcggtcaccg aactcggtcc cgctatcctg
     2701 gcaacgggga tcgcagccac agcgttctcg ctgtggctgt tcattttgtt tttcagaggc
     2761 tcagagtccc gccggataca gcgcgagata agcggatatc agcaggcact gagggaggca
     2821 gaagaggctc acagagccag acaaaatacc cttattcaga atcacaacct ggagacagcg
     2881 gccctgcgaa gccactacat tggtgctgag gatgtactgc gggaagcgtt tgcgcaaaaa
     2941 gaaagcctct tgcttcaggc ggtgcagaat gcccggaaag aagccagaaa ggagctggaa
     3001 gaacagaggg aggcactgaa tcagcgcgaa tccatcctgc agaaaaagga gctggcgctg
     3061 gaaacaaccc tggagaaggc ccggaaatat gtggccgggg agcggcataa caccgagctg
     3121 gcccgtctgg aagcagagaa agcagaggaa agacggagaa atgcctgcgc gacggcggaa
     3181 cggcgcagac ggaaactgga aaaactccag gccactggcg gaacagcagg gcaaaaacac
     3241 tgaggcattt caggactgcc agaaacgcca ccagggccgg gtcgtgttct gccgctgatc
     3301 ttcccggttc tccagcaaca acatcgcctg tttcagactg tcgatatgtg aatcttttga
     3361 ggcaagaagt gtgttcaggt gttcaatttc ctgtttcaga gtatgaatga cctgttcatt
     3421 atggtcagaa gtgactgtgt cacgctgtcc actgacctgt tcacccctct gttcaacggg
     3481 tacagacgca aaggcaccaa acacccgcaa aagctctgaa gtctcgagca ttttcgcatt
     3541 ccgttcaccc cgacaggtgg acagctcacc ggctttaatt aacctgtgca gggttgtcct
     3601 gctcttaccc gtcaggcgtg cagcctcact gatcgacact gtcgccatca gtcaccctcc
     3661 tgtagtgcat ccagtctgag ggacagtgta cggaaaagat gctccgcatc atcgtggagt
     3721 ttctcacaaa ggtcagcctc cgcatcgaga tcaagctcgt taagcttctc cagcaggagc
     3781 agggactgat tctggatgaa tttagccatg ttcagggcag ttttctgttg tttcgtcata
     3841 aggggcctca gggcgtcagt gctcagtgta aaaccggtct cttttccgct tcctcgctca
     3901 ctccgtcgct acgctcctgc cgttcggctg cggcgagcgg acgggacaca cagaagagtc
     3961 atcgcgataa cagggaagaa aagagcctgt ggaaaaagcc acggcgttgc cgtttttcca
     4021 taggctccgc ccccctgaca agcatcacag aatctgacgc tcaaatcagc ggtggcgaaa
     4081 cccgacagga ctataaagat cccaggcgtt tccccctggt agctccctcg tgcgctctcc
     4141 tgttcctgcc tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc
     4201 cacgcctgac actcagttcc gggtaggcag ttcgctccaa gctggactgt atgcacgaac
     4261 cccccgttca gtccgactgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4321 taagacatgc aaaagcgcca ctggcagcag ccactggtaa acaggatgtg cgagagatgt
     4381 agtaagagtt cttgaagtga tggcctgagt gcggctacac tgaaaggaca gatttggtga
     4441 catgtctcgc acaagactgt taccgcggtt agaaaatctg ccaggattaa ctaaccttcg
     4501 aaaaaccacc tgccagggtg gttttttcgt ttactgagca agagattacg atgccaaaga
     4561 aaggatctca agaggatcct gttccatatg cctacccctg aagtgaaacc agcaccactc
     4621 cgcgacacca actatcaaaa taaaattatt gaactcattg attttaataa gaaaaaaaac
     4681 agatataatc atgatccaca atcacccaac ttgggtaata aaatcaatga tttataggtt
     4741 aggtatttta tcaatatcgc tgcttactgt tggttgtgtt tctaccagca acgttcttga
     4801 gttaggtagg gatacatata cggtttcagc aactgctgat ggttttagag atgcggcatc
     4861 agcacgtcaa agtgccttta aaacaggttc tgagaaatgt aatagcctcg gtaaaaaatt
     4921 cctgctcact aacgaaaacg tggctaacac aagaatgggg atagatacca cagtaacact
     4981 ctatttccgt tgtcttgacg aaagagacag cgagtatgta cgaccaaatc tacagaaagc
     5041 accagatcta ctcatcgaga gtaagtaatt atgaatttcc ttatgatcat gatcctatgg
     5101 tggatatcgt cattcatcac acacatgatg aaagttggaa atgtcagaga gtacggtccc
     5161 actaaaagcc atttgctgtt aatggttaca acgcttcctg ctggcgtagg tgtttttatc
     5221 tatccgatag tagaggtgat atggggagag ctgtcgttac tgaaagttgc tggttatacc
     5281 tttttatccc tcattgttgg cggacttctt gccaatatca ttgttcgcgg attatctgcg
     5341 acaacattcg gcattttgct cgtaaaccca ttagcaatga tatgcagcat tgccggactg
     5401 atagttcttg ctatccaaca tttaccataa tcccttctta cgcgcaccac caccacacca
     5461 ctcatcgcca aaatccgccg gaacgtccaa aagtgcaggt tttacacacc gaaatcgggc
     5521 tatttttgtg ttgccggttt gttatcaaaa tggcgagact ggtttcagcc tgtttacaga
     5581 ctgaaacctc tatttcttcc agggtaatga ctcaaaatta ttgatagtgt tttatgttca
     5641 gataatgccc gatgaccttg tcatgcagct ccaccgattt tgagaacgac agcgacttcc
     5701 gtcccagccg tgccaggtgc tgcctcagat tcaggttatg ccgctcaatt cgctgcgtat
     5761 atcgcttgct gattacgtgc agctttccct tcaggcggga ttcatacagc ggccagccat
     5821 ccgtcatcca tatcaccacg tcaaagggtg acagcaggct cataagacgc cccagcgtcg
     5881 ccatagtgcg ttcaccgaat acgtgcgcaa caaccgtctt ccggagccaa cagccagcgc
     5941 tggcgcgatt tagccccgac atagccccac tgttcgtcca tttccgcgca gacgatgacg
     6001 tcactgcccg gctgtatgcg cgaggttacc gactgcggcc tgagtttttt aagtgacgta
     6061 aaatcgtgtt gaggccaacg cccataatgc gggcggttgc ccggcatcca acgccattca
     6121 tggccatatc aatgattttc tggtgcgtac cgggctgaga ggcggtgtaa gtgaactgta
     6181 gctgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggca gtgcttttgc
     6241 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
     6301 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgggc
     6361 aacgcggagg aaggttcatc aggaaaaaaa ctgatttttt cgaaaaaacg tcctacatat
     6421 tgaatatgta acctttttac atataatata acattaaatt aattttttct ttaaagggta
     6481 gcatgatatc agttaattta actacaatgc attctcgact ggatttatgt gccgccacag
     6541 tgtggtcaat tataaaccag agtgttcagc cagataaaat aaacctttgg atatcaaatg
     6601 agccatattt gtcagatcat gggatatgtg agatacctgc ttgggttgaa gagctaaata
     6661 aaataaataa catagtgaat gtacattatg taaaaaatac cggaccatac cgtaaaataa
     6721 ttaacgcaat tattaactca gagattgatg acattcttgt atatgctgat gatgatgtaa
     6781 tatatggtca gcagtggctt gagttcctat taggccagtt ttatgagaac aactgtaatg
     6841 tggcaattgc tgctaggatt agaataatgg agaggaatat ttttaacaag tacaaatctt
     6901 atagctctta ttctattagt atgaaaaagg ataaatttga aaaggatttt ataataactg
     6961 gcgttggggg ttgtgtttta agtaagagaa tgattaagcc tagcattatc ggagatgatg
     7021 catatttaac tatttcacct aagacagatg atatctggat tagtaaaatg ttagaggctt
     7081 ccaatacgaa ggtcgagtca tccccagatg cactgcgatg tgttcatgaa atattgcatg
     7141 gctataacac acttagctca ataaatacat taacatctaa aaatagcagt ttaattataa
     7201 aggcaatcag gatggttaaa aacaaattct attcatattt tgggttgcaa aaaacaaaca
     7261 atgacaaatc catcaagatg gttaatttac attttaatgg taaattataa atgatctttc
     7321 tcatcatgga ttgtctgaaa acaaaatgtt atcagtaaac tgtagtggtt agcaaattgt
     7381 tattgctttt ttgggtaatg atgccaattt attgatttcg cattttcact gacgccagga
     7441 tcacctgata ttacaagggt tgaatggcta cagattcaaa tggtcaccaa caggttggca
     7501 gcattacttt gggataggct ctaacgctga aaggtataaa aaattccttt tagtacacca
     7561 atgtaattat tttatatcac tgacaataaa tatgaattta aaatcgaaaa caatacatgc
     7621 tgtgaagtgg aatttgttgt ctacagtgtg cgtaaccggg cttggtatgg tctcactgtg
     7681 gatcttgtcc agcttattta caacgacaga ttatggtatc attagtgcgg cattaatatt
     7741 aagtactgtt ttctcaatca taactgattt tggtatatca aactcagtta taagatctga
     7801 aaagttaaat aaatatgaac tgtcttcact gtatataata aatgtctttt taggtatgat
     7861 attttgtctc ggcttgttta tttttagtgc tcagttagca tccttattca atggtggaac
     7921 agaactggca aaacagataa aaatcatgtc attttctctt ataatatcgt ctttcggtgg
     7981 acagccaaga gctttgttag ccagagaact tagatttgat gtaatatcca ggataacaat
     8041 cctgtcagct atttttaatt ttgtgttcgc agtcttctta agctacgtct atcgttcccc
     8101 ttggtgtgtt gccgtttcac tgttggtttc ctcatctatt agtagcgtat tgtatatata
     8161 ttcaagcaaa ggcttgagag atattaagtt acagttcttt actttgaagg aaataaaaaa
     8221 acatgtcagc tacggttctc agttggtcat ggactctatc attaatcaga taagcgtgaa
     8281 tacatatcct gtactaatgg cacgcttaat aagtttgact gctatcggtg gatataatat
     8341 atcctacaac attagcgtgg cgctttttga aaaattgaat cctgttttat cacatgcgtt
     8401 atttccagct ttttccaaga tacaaaatga tgcagtatct ctgaaaaaaa cttttctaaa
     8461 agtaactatg ttttcgtctt tggtgaattt cccgatgtta ttgggaatga tgttaatttc
     8521 agactatgta gtgactgttt tttttgagca gaaatggcag ttcatcaccc ctattgtaca
     8581 gattttatgt ttggttgggg cggcgcgatc ccttgatgtg cctgttatct ctgtcttgct
     8641 tgctaaaggg caaatgtaca gaaatgttta cctgggagta tttaagttag cgcttggtat
     8701 tcctttaaca tggtttctgg gtaaacaata tggtcttaca gggatagtgt tttcattctt
     8761 atgtattcaa atagtaaatc ttgttatggg atattttttc caggttagac cctgtttaaa
     8821 tatcaaagta aagagttaca tctatgccgt cttcgtaccg cttatacaca ctttacctat
     8881 gcttgtaacg ggttacctgt tgattctttt tttaactatt acctctccta ttttgtcaat
     8941 ggttgtgatt attttttcat gtatgtctgt atacattgct tctatactca cctctccagt
     9001 aaaagagata aaggaattta acgcaatcgt tctaaacaat atactgagga ataaatgatg
     9061 ttattatcaa taatcatacc atgttataac tgtgagtcaa atatcgaaaa attgcttctc
     9121 tcaataaaag agcagcttaa tgaaaccgta caactagtgt tgattaatga tggctcatgg
     9181 gacgcaacgc atgacataat aactaaattt acaagtgaca acccctctat aaaaataaaa
     9241 tacattaata gtattaatca gggggcggca aaagccagaa caacgggctt aaattacgca
     9301 gagggagatt atgtattctt ctgtgattca gatgatatga tttctgctaa tttcatatcg
     9361 gtttttacag agttaagtaa tgataaaccg gatatgatat atttttgctc tacggtatta
     9421 gattcgcaag gaaacaggtt taaaaaggta cattttccat tagcatacat cgaaagcaat
     9481 agtaacgaag tttttaatta tcttttgaag aaagggcagt ggacagctgc tgtatggact
     9541 tatattttcc ggaaagatct cgtattgaaa tctggcgcta tatttacaga cagaaaggta
     9601 catgaagatc acttattcac gctgagactt ctcgctggta gtgaaaaaat atcaatatgt
     9661 aattctgagc tttactttca gaatgtgact tcaggttcac ttactaaatc ccctaaaaaa
     9721 attgattatt taactgagcg ttataagtct tataaagagg ccagggaaga tatcatttac
     9781 aaattcagtc aggaaagcat tgttctttat gatcgatggt ctatgtcttc gctactgcaa
     9841 atattaaaag aaaataaaaa aatggtgttt caggggataa tgcatacagc tatatatcga
     9901 gtattgtgga aggaaaaatt agtgatttta gattttgtta aaagcaaagt taaaaaaaaa
     9961 atcacgagat ctaagagcct atcccaata
//
