LOCUS       NZ_CP084815             4895 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124
            plasmid pKqq_SB1124_9, complete sequence.
ACCESSION   NZ_CP084815
VERSION     NZ_CP084815.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024802
            Assembly: GCF_020525825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name :: Klebsiella quasipneumoniae subsp. quasipneumoniae
            SB1124
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:46:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,784
            CDSs (total)                      :: 5,657
            Genes (coding)                    :: 5,426
            CDSs (with protein)               :: 5,426
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 82 of 231
            Pseudo Genes (incomplete)         :: 153 of 231
            Pseudo Genes (internal stop)      :: 38 of 231
            Pseudo Genes (multiple problems)  :: 36 of 231
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084815.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB1124"
                     /host="Water (environment)"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKqq_SB1124_9"
                     /geo_loc_name="Netherlands"
                     /collection_date="2002"
     gene            267..533
                     /locus_tag="LGM22_RS28805"
                     /old_locus_tag="LGM22_28805"
     CDS             267..533
                     /locus_tag="LGM22_RS28805"
                     /old_locus_tag="LGM22_28805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136069852.1"
                     /translation="MKIVLSVSKCLYINDLDRLLPCDRFFVHLDYRDISKKESFEMNRH
                     DKETYKLIFDKLIGNDVMLAIDEDIYSEVVENLLYYSNKTYGD"
                     /name="hypothetical protein"
     gene            540..806
                     /locus_tag="LGM22_RS28810"
                     /old_locus_tag="LGM22_28810"
     CDS             540..806
                     /locus_tag="LGM22_RS28810"
                     /old_locus_tag="LGM22_28810"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_119913217.1"
                     /translation="MIDEMTQYRIRRFTQSQKSLLLKMIKESQRSDLEDNIKDILKDID
                     VSYSTGITKKASRKEEMIACREVHYIFSILTKRGINNDYRNEI"
                     /name="hypothetical protein"
     gene            808..1059
                     /locus_tag="LGM22_RS28815"
                     /old_locus_tag="LGM22_28815"
     CDS             808..1059
                     /locus_tag="LGM22_RS28815"
                     /old_locus_tag="LGM22_28815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226683691.1"
                     /translation="MKLNVLARFFYYIEQDKDIPFDYSSYDEQGLCYFVANRYINENKA
                     DDLIQALIDTNDDDYIKAIRDYVQYTALNEVRKKYEDR"
                     /name="hypothetical protein"
     gene            1046..1417
                     /gene="mobC"
                     /locus_tag="LGM22_RS28820"
                     /old_locus_tag="LGM22_28820"
     CDS             1046..1417
                     /gene="mobC"
                     /locus_tag="LGM22_RS28820"
                     /old_locus_tag="LGM22_28820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_064374444.1"
                     /translation="MKTDRKRGRIELNHEVRRDISIQFRVNKAEFEYLKELVEASGLER
                     GHYIRREILNSEPIVKRKMPDFEQSLYKEIQHQGNNINQIAKKLNTNEDFKQSVAAEAN
                     FAIKQLMKRILKACEVYTI"
                     /name="mobC"
     gene            1414..2898
                     /locus_tag="LGM22_RS28825"
                     /old_locus_tag="LGM22_28825"
     CDS             1414..2898
                     /locus_tag="LGM22_RS28825"
                     /old_locus_tag="LGM22_28825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_136069851.1"
                     /translation="MKGNVAKPGRSFKNRVDYILKDDHDFICSNIASDYNNVSDLTDEF
                     KTVSSFRQDIKKPVFHAFLSLPKNEKLTDEQWQEIAKDYLKEMNIDIDKHQYICVRHND
                     TDKEHIHIVANRVGLDGSVWLGQHSAFNTIAACERLEVKHGLTITDGLKGQKSEVSAPT
                     KNEIEMALRTGEKPARLVLQASLQAAMTGKPDLETFVERLQAVGIEPRFNVASTGNVAG
                     CSFSVGDVAFKGSQLGKKYSWNTIKGKVIYDKNRDDELIRRFAARKTDDENSIGRSPIE
                     PNINDDRTIIRNDDDTIGIGQYSIKPDTTDTYEGKQLVRSNHNSFKLSEKYNRPTGPKV
                     RRTTQKAAQILNRVKKQYNKEPIKTYNKNGLNSWRDKSDSGSIGRILDHAETANAATYS
                     FRLKSGSRFNSRATKIEEIRDKLGIKKPVIQPKNKEKIVKEEIKKKPLGLSMNENRFKS
                     KLKETLKKEKPRNETTPTQLQQEDDQHLKPRGFKPR"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(2907..3179)
                     /locus_tag="LGM22_RS28830"
                     /old_locus_tag="LGM22_28830"
     CDS             complement(2907..3179)
                     /locus_tag="LGM22_RS28830"
                     /old_locus_tag="LGM22_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_023312618.1"
                     /translation="MKVKNRKRELTAKEYAEQYEISVRTVKRYFSQDREDYEKEAKQRR
                     LKAFVLRESGMKWAEVGIALGTTKHGAIALYKRYQQIDAPTTKEA"
                     /name="hypothetical protein"
     gene            complement(3176..4027)
                     /locus_tag="LGM22_RS28835"
                     /old_locus_tag="LGM22_28835"
     CDS             complement(3176..4027)
                     /locus_tag="LGM22_RS28835"
                     /old_locus_tag="LGM22_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000481810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_064167154.1"
                     /translation="MFNIENNREILKSQIPLKPYATNDLEFGLTIQNKNKALDMLYLQV
                     NHPLYLHTMIFDLDKENCFYEFENAHLPIPSFITKSPDSGRCHYGYMLNAPISSTEKSR
                     QKPVKFARALYYNMATRLGADLGYAGLITKNPLHSHWSPFWSGADLYELNDLADCFDDL
                     EDPKKRENTGLAFGRNVEMFDTIRQWAYKNVLKYQSESSFNDFHNELLLKCQMHNAYLN
                     ADDLLPYNEIKATAKSIAKFCWKEFSEEKLNKIQSKKQSYRGKKNKGIVKSKTRKFLEA
                     IK"
                     /name="replication initiation protein"
     gene            4323..4547
                     /locus_tag="LGM22_RS28840"
                     /old_locus_tag="LGM22_28840"
     CDS             4323..4547
                     /locus_tag="LGM22_RS28840"
                     /old_locus_tag="LGM22_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001171587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_064167156.1"
                     /translation="MHEQKDKKRRLGFMIGKSNATLPADEQTEAELDQEVLKLFYPNQC
                     TKDTIERSEAGTDVFTASGSDDLFKKLKL"
                     /name="hypothetical protein"
ORIGIN
        1 cagtgtattt atttatgtag ccgctattga ctagtttttt agatcggtta tttatttaaa
       61 tataagttga gttcagcttt tattttttag ctttcggaaa ctgaggcaag gtgtaatagc
      121 gtatccgcta ttttacccaa tcctatcgga ttgccttgtc tcgtttcact cgcaaggttg
      181 aaaaagatct acccttccgc ttatttcccc tatcaagggg aagcggtaaa ccatagttag
      241 atattataaa tataggagaa ataaatatga aaatagtttt atcagtatca aaatgtttat
      301 acataaacga tttagatcgc ttacttccgt gtgatcgttt ttttgttcat ttagattata
      361 gagatattag caaaaaagag tcatttgaga tgaatagaca cgacaaagaa acatacaaac
      421 ttatctttga taagcttata ggtaatgacg taatgcttgc tattgatgaa gacatctaca
      481 gcgaagttgt cgaaaactta ctttattatt caaataagac ttatggagat taatataaaa
      541 tgatagatga aatgacacaa tacagaataa gacgctttac acaaagtcaa aaaagcttgc
      601 tactaaagat gataaaagaa agccagcgaa gcgatttaga agataatata aaagatattt
      661 taaaagatat tgatgttagt tattcaactg gcataacaaa gaaagcatca agaaaagaag
      721 aaatgatcgc ttgtcgtgaa gtccattaca tctttagtat attaacaaaa agaggcatta
      781 acaatgatta tagaaatgaa atatgaaatg aaacttaatg tattagcacg ttttttctat
      841 tacatagaac aagataaaga tatacctttt gattactcaa gctatgacga acaaggatta
      901 tgttattttg tagctaatag atacattaac gaaaataaag ctgatgatct tatacaggca
      961 ttgatagata ctaatgacga tgattatata aaagctataa gagattatgt tcagtataca
     1021 gctttaaatg aagtgagaaa gaaatatgaa gacagataga aaaagaggac gtatagagtt
     1081 aaatcacgaa gtacgtagag atatatctat acagtttaga gtaaacaaag cagagtttga
     1141 atatttaaaa gaacttgttg aggcgtctgg ccttgaaaga ggtcattaca ttagaagaga
     1201 gattttaaac tctgaaccta ttgttaaaag aaaaatgccg gattttgagc aatcattgta
     1261 taaagaaata caacatcaag gtaataatat aaatcagatt gctaaaaagc taaatacgaa
     1321 tgaagacttt aaacaatctg tagccgcaga agctaacttt gctataaagc aacttatgaa
     1381 aagaatatta aaggcttgtg aggtttatac catatgaaag gaaatgtagc aaaacccgga
     1441 agatcattta aaaatagagt tgattacatt cttaaagatg accacgattt tatatgtagc
     1501 aatatagcaa gtgattataa taatgttagt gatttaactg atgagtttaa aactgtaagc
     1561 agctttagac aagatataaa aaaaccagta tttcacgctt ttttatctct tccaaaaaat
     1621 gagaagctca cagatgaaca atggcaagaa atagcaaaag actatttgaa agaaatgaat
     1681 attgatatag acaaacatca atatatttgt gttcgtcata acgatacaga taaagaacat
     1741 atccatattg tagctaatcg tgttggcctt gatggttctg tatggctcgg tcagcactca
     1801 gcatttaaca caatagcagc ttgtgaacgt ttagaagtaa aacacggctt aaccatcaca
     1861 gatggcttaa aaggtcaaaa atcagaggta tcagcaccaa ccaagaacga gatcgagatg
     1921 gcattgagga caggagaaaa gccagcccgt ttagttcttc aagcctctct acaagcagct
     1981 atgacaggta aaccagacct tgagacgttc gtagaacgtc tgcaggccgt aggcattgaa
     2041 ccaaggttca atgttgccag cactggcaac gtggcgggtt gttctttcag tgtgggagat
     2101 gtggcattta aaggctcaca gttgggtaaa aaatacagtt ggaacactat taaaggcaag
     2161 gtgatatatg acaaaaatag agatgatgaa ctcattagaa gattcgcagc aagaaaaaca
     2221 gatgacgaaa atagtattgg acgatcacct attgaaccaa atatcaatga cgatagaacc
     2281 attatcagaa acgatgatga tactatcgga atcggtcagt acagcattaa accagacaca
     2341 acagacactt acgagggtaa acaactcgtt agaagcaacc acaacagctt taaactcagc
     2401 gaaaaatata atagaccaac aggcccaaaa gttagaagaa caacacaaaa agctgcacaa
     2461 atactcaaca gagttaaaaa acaatacaat aaagaaccaa tcaaaactta taataaaaat
     2521 ggcctcaata gttggcgtga taaatctgat agcggttcta ttggtaggat acttgatcat
     2581 gcagaaacag caaacgcagc cacctattca tttagactca agagcggtag ccgtttcaat
     2641 agtcgagcaa ctaagatcga agagataaga gataaacttg gaataaaaaa acctgttatt
     2701 cagccaaaga ataaagaaaa aatagttaaa gaagaaataa agaaaaaacc tttaggtttg
     2761 agtatgaatg aaaatagatt taaatcgaag ttaaaagaaa cccttaaaaa agaaaaacca
     2821 agaaatgaaa caacaccaac acaactacag caggaagacg atcagcattt aaaaccaaga
     2881 ggttttaaac cgcgataaaa gaggctttaa gcctcttttg ttgttggtgc gtctatttgt
     2941 tgataacgtt tataaagagc tatagcacca tgttttgtag ttccaagagc aataccaacc
     3001 tctgcccatt tcatcccgct ttcacgtaat acaaaggctt ttaatcgtct ttgttttgct
     3061 tctttctcat agtcttcacg atcttgagag aaatatcttt ttactgttct aacgctaatc
     3121 tcatactgtt cagcatactc ttttgcagta agttctcttt ttctattttt aactttcatt
     3181 ttatggcctc caaaaactta cgagttttac tttttactat tcctttgttt ttctttccac
     3241 ggtaagactg ttttttactc tgtatcttgt tgagtttttc ttcgctgaat tctttccaac
     3301 aaaactttgc aatgcttttt gctgtggctt ttatctcatt atatggcaat aaatcatcag
     3361 catttaaata tgcattatgc atttgacact ttagcaatag ttcattgtgg aaatcattaa
     3421 atgagctttc actctgatat tttagcacat ttttataagc ccactgcctt attgtgtcga
     3481 acatttcaac attacgacca aaagcaagcc ccgtattttc tcgttttttg gggtcttcta
     3541 aatcatcaaa acaatcagct aaatcattaa gttcgtataa atcagcacca gaccagaacg
     3601 gcgaccaatg gctatgcagt gggtttttag taatcagtcc cgcatagcca agatcagcac
     3661 caagacgagt agccatattg taatataatg ctctggcaaa cttgacaggc ttctgtctgc
     3721 ttttctctgt tgatgagatc ggagcattaa gcatataacc gtaatgacat ctaccagagt
     3781 ctggtgattt tgttataaaa gacgggatgg gcaaatgagc attttcaaac tcataaaagc
     3841 agttttcttt gtctaaatca aatatcattg tatgaagata caacgggtga tttacttgta
     3901 aataaagcat atctaaagct ttattcttat tttgaatagt caatccaaac tctaaatcat
     3961 ttgtagcata tggctttaaa ggtatttgac tttttaatat ctctctgtta ttttctatat
     4021 tgaacattta gcagtatcct ttctgtaatg ccccgttctc tcactgtcgc caaactaatc
     4081 agagaacgcg ggcttttctt ttatttataa tctcttacag ttttactata ccaaaaaaac
     4141 ttgtcaatag atctaccaaa aaaaagttgt tatgtgtgcc attaaccaat atcaggtatt
     4201 agacggagtt ttggggatac tacaccacag cgaagcacag gccgaaggct actgcagagt
     4261 aagtcacact gtaacgtata ctaaactcta tgcaggatct ggctgtgctg gggaggtaca
     4321 tcatgcacga acagaaggac aagaaacggc ggttaggttt tatgattggt aagagcaatg
     4381 caacattgcc agcagacgaa caaacagaag cagagcttga tcaggaagtc ttgaagctgt
     4441 tttatcctaa ccaatgcacc aaagacacca tagaacgctc agaagctggt acagacgttt
     4501 tcacggcctc cggttctgat gacctgttca aaaaattgaa gctgtagagg gtaggggaga
     4561 gctggccatc ttcaccactt gagaacaaac cgacccggaa cggtgagaga aggccaaccc
     4621 tcgatgcagt atatactaat atatactata gtctgttgat tttatgagca ttttaaaccc
     4681 atagacgcca gcaaacgagc aaaagcaagt tttactcgtt tctgtcgccg ccgatcaaac
     4741 cccattcctt aaaatctggt gatctgatgg tcacgaactt ttccagattt cacgtagcag
     4801 cagcattttt tacgcaaagc tacctattac gcacagctac ccatcaagac aatatattat
     4861 ttaccctatt ttttatttgc attttatttg tcttg
//
