LOCUS       NZ_CP084812             7650 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain SB1124
            plasmid pKqq_SB1124_6, complete sequence.
ACCESSION   NZ_CP084812
VERSION     NZ_CP084812.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024802
            Assembly: GCF_020525825.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 7650)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7650)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name :: Klebsiella quasipneumoniae subsp. quasipneumoniae
            SB1124
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:46:47
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,784
            CDSs (total)                      :: 5,657
            Genes (coding)                    :: 5,426
            CDSs (with protein)               :: 5,426
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 89
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 231
            CDSs (without protein)            :: 231
            Pseudo Genes (ambiguous residues) :: 0 of 231
            Pseudo Genes (frameshifted)       :: 82 of 231
            Pseudo Genes (incomplete)         :: 153 of 231
            Pseudo Genes (internal stop)      :: 38 of 231
            Pseudo Genes (multiple problems)  :: 36 of 231
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084812.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7650
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="SB1124"
                     /host="Water (environment)"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKqq_SB1124_6"
                     /geo_loc_name="Netherlands"
                     /collection_date="2002"
     gene            join(7256..7650,1..301)
                     /locus_tag="LGM22_RS28890"
     CDS             join(7256..7650,1..301)
                     /locus_tag="LGM22_RS28890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="WP_228757872.1"
                     /translation="MCISYLKEDRSIFFSSVITSLYTTSGIVILGAVSSNQEVGYYTSA
                     QKLIDVTKSIMLMPISQVIFPILSEKFGKNRMDGIKAVQSIMPAFFLFSLIVLIFMISF
                     GGIVLEVIFGGDFSPASPMLTILSFGLFAVFYGVLIGGQVMLNLGMDKEFLNVQVIVSV
                     ASLAANYLILPFGGGVTISIVWTLSEFFISAYQIMILKKNGISIIGFWCINPRLIIASV
                     KKILNRKEG"
                     /name="oligosaccharide flippase family protein"
     gene            308..1216
                     /locus_tag="LGM22_RS28695"
                     /old_locus_tag="LGM22_28695"
     CDS             308..1216
                     /locus_tag="LGM22_RS28695"
                     /old_locus_tag="LGM22_28695"
                     /inference="COORDINATES: protein motif:HMM:NF017515.4"
                     /GO_function="GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="WP_136069845.1"
                     /translation="MNITKAVNAIRAVSAVLSEKDKSPMIKWIEINTDSKREYKEYSIP
                     KKIWIYWDSITPPSIVDLCVHQASKVCADYEVTLLNPSNLNEYISLPEISSDIPASNKA
                     DLIRLMLIEQYGGIWMDASIFLTEDLEWITSKLDRHESFLFYSDECTKDTRRPISENWF
                     IAAPIGSDFIKNWKEEFLSCITSKKPKSFYNDMEDRDYHLQGLTKPDYLLCYISAIVVL
                     NRKKYNIIYASSGSTGHYLNYKHKWNGIAIAAELLLRNKKNIAITKLIKLNSSSRRSVQ
                     KFIDMKIIRKNSILGFHLKNK"
                     /name="capsular polysaccharide synthesis protein"
     gene            <1406..1518
                     /locus_tag="LGM22_RS28700"
                     /old_locus_tag="LGM22_28700"
                     /pseudo=""
     CDS             <1406..1518
                     /locus_tag="LGM22_RS28700"
                     /old_locus_tag="LGM22_28700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /name="IS5/IS1182 family transposase"
     gene            2630..2851
                     /locus_tag="LGM22_RS28705"
                     /old_locus_tag="LGM22_28705"
     CDS             2630..2851
                     /locus_tag="LGM22_RS28705"
                     /old_locus_tag="LGM22_28705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_016947202.1"
                     /translation="MQFDTAAELAALQAQTRRIRQVRYRPSRLDRYTGELLSLYQAGAS
                     AAELQRWLRARRIKVVLSTVTRWLEKNG"
                     /name="hypothetical protein"
     gene            2844..3731
                     /locus_tag="LGM22_RS28710"
                     /old_locus_tag="LGM22_28710"
     CDS             2844..3731
                     /locus_tag="LGM22_RS28710"
                     /old_locus_tag="LGM22_28710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="WP_048290304.1"
                     /translation="MAKFASAARQAAGVIKPLQGNILRSVGTVRNYEQGLTRVAEWMKA
                     NRINHGDLRTLTPAQAITYLEQRGEQVGQKTLDMERQAIQAMMIHVTQTLPAGEHLPVV
                     KSEQAQILSSRAYSPDQVALIAAAQQPANALATAIALAAGLRAHELLTLAPRQERAADP
                     RPAADSKFAGREGVIYTVQGKGGLVREVLLPSRLAERLEHVRLASPVRVTDRGVFYQSQ
                     YAIGGGQRWSNAFSAASMRTLGWSRGAHGVRHSYAQQRMQELQKLGLVRDMALRTVSQE
                     MGHFRPEITETYLR"
                     /name="integrase"
     gene            3728..4426
                     /locus_tag="LGM22_RS28715"
                     /old_locus_tag="LGM22_28715"
     CDS             3728..4426
                     /locus_tag="LGM22_RS28715"
                     /old_locus_tag="LGM22_28715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016947204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_048290303.1"
                     /translation="MTRTFILLCTGVFAIAYALYELNHYGVYYPDVSNPEAFTGWKAVT
                     ELGPAILATGIAATAFSLWLFILFFRGSESRRIQREISGYQQALREAEEAHRARQNTLI
                     QNHNLETAALRSHYIGAEDVLREAFAQKESLLLQAVQNARKEARKELEEQREALNQRES
                     ILQKKELALETTLEKARKYVAGERHNTELARLEAEKAEERRRNACATAERRRRKLEKLQ
                     ATGGTAGQKH"
                     /name="hypothetical protein"
     gene            complement(4454..4831)
                     /locus_tag="LGM22_RS28720"
                     /old_locus_tag="LGM22_28720"
     CDS             complement(4454..4831)
                     /locus_tag="LGM22_RS28720"
                     /old_locus_tag="LGM22_28720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016162088.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="WP_136069844.1"
                     /translation="MAKVSISEAARLTGKSRTTLHRLIKAGELSTCRGERNAKMLETSE
                     LLRVFGAFAPVPVEQRGEQVSGQRDTVTSDHNEQVIHTLKQEIEHLNTLLASKDSHIDS
                     LKQAMLLLENREDQRQNTTRP"
                     /name="helix-turn-helix domain-containing protein"
     gene            complement(4831..5022)
                     /locus_tag="LGM22_RS28725"
                     /old_locus_tag="LGM22_28725"
     CDS             complement(4831..5022)
                     /locus_tag="LGM22_RS28725"
                     /old_locus_tag="LGM22_28725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_016947206.1"
                     /translation="MTKQQKTALNMAKFIQNQSLLLLEKLNELDLDAEADLCEKLHDDA
                     EHLFRTLSLRLDALQEGD"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            5433..>5513
                     /locus_tag="LGM22_RS28730"
                     /old_locus_tag="LGM22_28730"
                     /pseudo=""
     CDS             5433..>5513
                     /locus_tag="LGM22_RS28730"
                     /old_locus_tag="LGM22_28730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333247.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /name="replication initiation protein"
     gene            complement(5813..6495)
                     /locus_tag="LGM22_RS28735"
                     /old_locus_tag="LGM22_28735"
                     /pseudo=""
     CDS             complement(5813..6495)
                     /locus_tag="LGM22_RS28735"
                     /old_locus_tag="LGM22_28735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /name="IS1 family transposase"
     Typing          5071..5542
                     /name="ColRNAI_rep_cluster_1987"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="89.121"
                     /coverage="91.0"
                     /evalue="9.9e-165"
ORIGIN
        1 tgctgttttt tatggggtgt taataggcgg gcaagtgatg ctaaacttag gtatggacaa
       61 ggaattcctt aatgttcaag ttattgtttc agttgctagt ctcgccgcaa attatttgat
      121 tttaccattt ggcggagggg tgaccatatc aatagtctgg acgctatctg aattttttat
      181 atccgcttat cagataatga tcttaaagaa aaatggaatt tccattatag gtttttggtg
      241 tataaatcca aggttaatta ttgcatcagt aaaaaaaatt ctaaacagaa aagaggggta
      301 ggttgccatg aatataacaa aggcagtaaa tgcaataaga gcagtgagtg cggttttatc
      361 agaaaaagat aagtcaccaa tgataaaatg gattgaaata aacactgata gcaagaggga
      421 atataaagaa tattccatac caaaaaaaat atggatttat tgggattcaa taaccccccc
      481 tagcattgtt gatctatgcg tacatcaagc atctaaagta tgcgctgatt acgaggtgac
      541 gctattaaac ccatctaatt taaatgaata tataagcttg cctgaaatat catctgatat
      601 cccagcatca aataaagcag atcttataag gctaatgcta attgagcagt atggtgggat
      661 atggatggat gcgagcatat tccttactga ggatttagaa tggataactt caaaattaga
      721 tcgtcacgaa tcatttttat tttattctga tgaatgcaca aaagatacaa gaagaccaat
      781 ttctgaaaac tggtttatag ctgcacccat tggaagtgat tttataaaaa attggaaaga
      841 agaatttcta tcatgtatca catcaaaaaa accgaagtca ttttacaatg acatggaaga
      901 tagagattat catttgcaag gactaactaa gccagattat ttattgtgtt acatatcagc
      961 aatagttgtt ctaaatagga agaagtacaa tattatatat gcttcttctg gaagtactgg
     1021 tcactatcta aactacaaac acaaatggaa tggaatcgca attgcggcag aactcctcct
     1081 taggaacaaa aaaaacatag caatcaccaa gttgataaag ttaaattctt catcaaggcg
     1141 ttcggttcaa aaatttattg acatgaagat aataagaaaa aatagcattt tgggttttca
     1201 tttgaaaaac aaatagattt ttttgggtga tgctggcttt gttgaataat agggtgcatg
     1261 ctgttctgat tgttctactt tagtagaaga tacttttgac tattacgaga gtcttcgatg
     1321 atggacttaa cctactgatc ttacgttgcg gaagaccgat gattttgtag ggtggtagca
     1381 aggtgcttat ttattcaaca aagccggctc actgaccctg cgtgactacg atggttaggt
     1441 tgcagaggct atgccatggt gctggcactg aacaaaatga cgaaagcagg tatgcccgaa
     1501 agcgtgagta tcgcctgaaa acccagcccg ctacggggat acttacccga aatctgattt
     1561 attcaacaaa gccacctatt taccataacg tcttcttacg cgcaccgcga aaacggcctc
     1621 agggcgtttc tggtggttaa cgggcaaaag gtggtggcaa gtacaccgaa aaccgccaga
     1681 aacgcgcctg atttatttta cgcatatgca tggctattca caccaccgca tagccggtga
     1741 tttcggtcga gaaaagggcc ccggacgtta aaagcataaa agaacgggcc atccaggccc
     1801 gtcaaatgag gacaactctg taaaaggacc gtcgcacctg gtgcggtgcc ggcgacaaga
     1861 aaaagatagt tcatatcagc ccctcaccgg agaaaaatat ttcctggcca cctcaaccgc
     1921 cacacgctgt gttcccgtta actcgcgcag ataacgcagg aacatcctgt tttcctctgc
     1981 atgcgcctcc tcactgccct gtgcgtggta ttccactatc gcgttcacca cggactcacc
     2041 gacctgcatc gtctgacctc atctgcactt tcatcacacc gtttcagatc ccgcctcctc
     2101 gccatgatgt gaaacggtga ccgggcaggc aaattttttg tctgtccggt caccgttggc
     2161 ggggtccggg gcggtcagtc ccggtgtttt ttattccgcc aaatcagcac ctgcgacccc
     2221 cgctatgtac gacgcataaa tgcgcctgta catagcgtgg gtcgctattg agcggagtca
     2281 ggcgcaacat ttaaaaaaca acattcacac gcacgaaaaa cctggctagt atttagtcaa
     2341 accatgagga ggcgcactct gacgcgcacc tgcctaccgg cagcaggtaa aacagccggg
     2401 tccctccggg gatactgtac cgggcagccc aatccgagaa tcctgagaaa acagctaaaa
     2461 tctgcgagac cggcaatcag gtcaggccat tccctggctt aagggacgtt atcagccgcg
     2521 agcagtccgg gctgaaccac gagaggttcg aacgagggcg atcggcgcat gccgcagtga
     2581 ccgttaacgt aacggccact gatgttctta cacccaatcc ggagcacaga tgcaatttga
     2641 taccgctgcc gaactggccg ccctgcaggc gcagacccgc cgtatccgcc aggtccgcta
     2701 ccgtccctcc cgccttgacc gctataccgg cgaactgctg agcctgtacc aggccggggc
     2761 cagtgcagcg gagctgcaac gctggctccg ggcccggcga atcaaggtgg tgttgtccac
     2821 ggttacccgc tggctggaaa aaaatggcta aattcgccag cgctgcccgc caggcggctg
     2881 gcgtgataaa accactccag ggaaatattc tgcgctctgt cggcacagtc cggaattacg
     2941 agcagggact gacccgcgtc gcagagtgga tgaaagccaa ccggattaat cacggcgatt
     3001 tacgcacact gacaccggcg caggccatca cctacctgga gcagcgcggg gagcaggttg
     3061 gccagaaaac gctcgatatg gaacgccagg ccatccaggc catgatgatc cacgtgacgc
     3121 agaccctccc tgccggcgaa cacctgccgg tggtgaaatc ggagcaggca cagatcctga
     3181 gcagccgggc ctacagcccg gaccaggtag ccctgattgc cgcagcacaa cagcccgcca
     3241 acgctctggc caccgcgatt gccctggccg ccggattacg cgcccatgaa ctgctgaccc
     3301 tcgcgccccg ccaggagcga gcagcggatc cccgtccggc agcggacagt aaatttgccg
     3361 gtcgggaggg tgtgatttac accgttcagg gcaaaggcgg cctggtcagg gaagttctgc
     3421 tgccgtcacg cctggctgaa cgtctcgagc atgtcaggct ggccagcccg gtccgggtga
     3481 ccgatcgcgg cgtgttttac cagagtcagt acgccattgg cggaggccag cgctggtcaa
     3541 acgccttcag tgccgcctcc atgcgtaccc tgggctggtc acggggggca cacggcgtgc
     3601 gtcacagcta cgcccagcaa cggatgcagg agctgcaaaa actcggtctg gttcgtgata
     3661 tggccctgcg caccgtcagc caggaaatgg gccattttcg tccggaaatc acggagacat
     3721 acctgcgatg acaagaacat ttattttatt gtgtaccggt gttttcgcca tcgcgtacgc
     3781 cctgtatgag ctgaatcact atggcgttta ctatccggac gtcagcaatc ctgaggcgtt
     3841 tactggctgg aaggcggtca ccgaactcgg tcccgctatc ctggcaacgg ggatcgcagc
     3901 cacagcgttc tcgctgtggc tgttcatttt gtttttcaga ggctcagagt cccgccggat
     3961 acagcgcgag ataagcggat atcagcaggc actgagggag gcagaagagg ctcacagagc
     4021 cagacaaaat acccttattc agaatcacaa cctggagaca gcggccctgc gaagccacta
     4081 cattggtgct gaggatgtac tgcgggaagc gtttgcgcaa aaagaaagcc tcttgcttca
     4141 ggcggtgcag aatgcccgga aagaagccag aaaggagctg gaagaacaga gggaggcact
     4201 gaatcagcgc gaatccatcc tgcagaaaaa ggagctggcg ctggaaacaa ccctggagaa
     4261 ggcccggaaa tatgtggccg gggagcggca taacaccgag ctggcccgtc tggaagcaga
     4321 gaaagcagag gaaagacgga gaaatgcctg cgcgacggcg gaacggcgca gacggaaact
     4381 ggaaaaactc caggccactg gcggaacagc agggcaaaaa cactgaggca tttcaggact
     4441 gccagaaacg ccatcagggc cgggtcgtgt tctgccgctg atcttcccgg ttctccagca
     4501 acaacatcgc ctgtttcagg ctgtcgatat gtgaatcttt tgaggcaaga agtgtgttca
     4561 ggtgttcaat ttcctgtttc agagtatgaa tgacctgttc attatggtca gaagtgactg
     4621 tgtcacgctg tccactgacc tgttcacccc tctgttcaac gggtacaggc gcaaaggcac
     4681 caaacacccg caaaagctct gaagtctcga gcattttcgc attccgttca ccccgacagg
     4741 tggacagttc accggcttta attaacctgt gcagggttgt cctgctctta cccgtcaggc
     4801 gtgctgcctc actgatcgac actttcgcca tcagtcaccc tcctgtagtg catccagtcg
     4861 gagggacagt gtacggaaaa gatgctccgc atcatcgtgg agtttctcac aaaggtcagc
     4921 ctccgcatcg agatcaagct cgttaagctt ctccagcagg agcagggact gattctggat
     4981 gaatttagcc atgttcaggg cagttttctg ttgtttagtc ataagaggcc tcagggcgtc
     5041 agtgctcagt gtaaaaccgg tctcttttcc gcttcctcgc tcactcagtc gctacgctcc
     5101 tgccgttcgg ctgcggcgag cggacgggac acacagaaga gtcatcgcga taaccaggaa
     5161 gaaaagagcc tgtggaaaag gccacggcgt tgccgttttt ccataggctc cgcccccctg
     5221 acaagcatca cagaaacgga cgctcaaatc agcggtggcg aaacccgaca ggacttaaag
     5281 ataccaggcg tttccccctg gtggctccct cgtgcgctct cctgttcctg cctttcggtt
     5341 taccggtgtc attccgctgt tatggccgcg tttatctcat tccacgcctg acactcagtt
     5401 ccgggtaggc agttcgctcc aagctggact gtatgcacga accccccgtt cagtccgacc
     5461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gactaatcgc
     5521 cattggcaac ggccactggt aaacaggatg tgcgagagat gtatcgagag ttcttgaagt
     5581 ggtggcctta ctgaggctac actggaagga cagatttggt gacacgtctc gcacaagact
     5641 gttaccacgg ttagatgatc tgccaggatt aactaacctt cgaaaaagcc acctgccagg
     5701 gtggcttttt cgtttacaga gcaagagatt acgatgccaa agaaaggatc tcaagaagat
     5761 ccttcaccat aatggaaatc tatccaaccc gggtaatgac tccaacttac agttagtgtt
     5821 ttatgttcag ataatgtcct atgaccttgt catgcagtac cgccgatatt gaaaatgaca
     5881 gcgacttcct gcccagcctt gccaaatgct gccttagatt caggttatgc cgttcaatgc
     5941 gctgcgtgta acgtttgctg ataacgtgca gctttccctt caggcgtgat tcatacagcg
     6001 gccaggcatc cgtcatccat accacggcat caaagggcga cagcaggccc agaagacgct
     6061 ccagcgtggc catagtgcgt tcaccgaaga cgtgtgccac aactgtcccc cgcatcctgt
     6121 catatgcgta aaacaaccag cgctggcgtg atttggcacc gacgtatccc cactgttcgt
     6181 ccatttcagc gcagacaata acgtcactgc ccggttgtat tcgcgaggtt accgactacg
     6241 gcctgagttt tttaagtgcc gtaaaatcgt gttgaggcca acgcccataa tgcgtgcact
     6301 ggcgcgacat ccgacgccat tcatggccat atcaatgatt ttctggtgtg taccgggctg
     6361 agaggcggtg taagtgaact gcagttgcca tgttttacgg cagtgagagc agagatagcg
     6421 ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg agcaggaagg
     6481 acagctgatg gaaatggaag taacgagagc acctcaagaa tactatcatt cactaaatca
     6541 gcaagttggg ggcattaccc agtcggctat agagactatt ccttaaacaa aacaaatctg
     6601 ataaaataag aaaaactcat ggcacatgat cataaaaaca gataatttat caatgaaaaa
     6661 aaacataaaa aatatttctt atatgtttct tgttcaatct gcaaattatt tattcccact
     6721 gataacaatt cccatcgtag ctagggcatt tggccctgac aagattggtt tgctaaacta
     6781 tataactgca atcataagtt actttatact cattacgtca tatggtttca acttcactgg
     6841 agtaagaaga ttatcgagaa acaaagatag gtcaaatgag atattttcaa caatcttcac
     6901 atcacaacta tatctaacca caatttgcac ttttatattc gcaatttgca ttcccattat
     6961 caacccattg aggggggatg tactgctttc aatagtaata ttcacatcat gcttgtctgt
     7021 tttatttacg caaacttggt ttttgcaagc atatagcgac ttcaaaacaa ttgcactatt
     7081 atccttttgc tatagattta cttcattcgt tctcattatt ttttttataa actcacccga
     7141 cgatgtaatt ctatatgcag ccataattaa catcggaacg cttgtttttt cattgttgtg
     7201 cttctttata acaataaaaa aatacaagtt aaaattaaga atcagcaagt tcagtatgtg
     7261 tataagttat ttaaaggagg ataggagtat ttttttctcg agtgttatta ccagtctata
     7321 tacaaccagt ggtattgtaa tacttggggc tgtatcttcc aatcaagagg ttgggtatta
     7381 cacatctgcg caaaaactaa ttgatgtaac aaagagcata atgctcatgc caatatcaca
     7441 agtgatattc ccaatcctct cagagaagtt tgggaaaaat aggatggatg gaataaaagc
     7501 agtgcaatct ataatgccgg cattttttct ttttagcctg attgttttaa tatttatgat
     7561 ttcatttggt gggattgttt tagaagtgat ttttggtggg gatttttctc cagcatcacc
     7621 aatgctaact attctttcat ttgggttgtt
//
