LOCUS       NZ_CP084800             3708 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain U41 plasmid
            pKqq_U41_11, complete sequence.
ACCESSION   NZ_CP084800
VERSION     NZ_CP084800.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024804
            Assembly: GCF_020525725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name :: Klebsiella quasipneumoniae subsp. quasipneumoniae
            U41
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,840
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 73 of 207
            Pseudo Genes (incomplete)         :: 139 of 207
            Pseudo Genes (internal stop)      :: 38 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084800.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="U41"
                     /host="Environment"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKqq_U41_11"
                     /geo_loc_name="Germany"
                     /collection_date="1990"
     gene            344..490
                     /locus_tag="LGM24_RS29985"
     CDS             344..490
                     /locus_tag="LGM24_RS29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071809246.1"
                     /translation="MHEPPVQSDCCACSGNYRLESKPERHDKAPLAAATGKRVYKRLLM
                     QSS"
                     /name="replication initiation protein"
     gene            complement(1688..1903)
                     /locus_tag="LGM24_RS29755"
                     /old_locus_tag="LGM24_29755"
     CDS             complement(1688..1903)
                     /locus_tag="LGM24_RS29755"
                     /old_locus_tag="LGM24_29755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="WP_000137422.1"
                     /translation="MTELEKQLLSALEQLQQDYSQRLDEWESAFAEWRSMCGLMQRENA
                     VLNERVSDLSTQVLSLSEQLRRLSGN"
                     /name="MbeD family mobilization/exclusion protein"
     gene            complement(1910..2401)
                     /locus_tag="LGM24_RS29760"
                     /old_locus_tag="LGM24_29760"
     CDS             complement(1910..2401)
                     /locus_tag="LGM24_RS29760"
                     /old_locus_tag="LGM24_29760"
                     /inference="COORDINATES: protein motif:HMM:NF016710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="WP_136070821.1"
                     /translation="MSSLLTLAKDLEQQSKAQKQSTGEMLKAAFSEHEQSVRAELSASA
                     RRISDAILAHEQSMSEAMEKNRRSVLRTAGRTWFTILMVSALLIGTSGSILWWQGQQIT
                     DNYTHLRQQEDTLAKMTARTWGVRYQESRDGRRFLILPPGMQTEAIPYDGTTWIRLKQE
                     "
                     /name="MbeB family mobilization protein"
     gene            complement(<2436..3140)
                     /locus_tag="LGM24_RS29765"
                     /old_locus_tag="LGM24_29765"
                     /pseudo=""
     CDS             complement(<2436..3140)
                     /locus_tag="LGM24_RS29765"
                     /old_locus_tag="LGM24_29765"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3079..3402)
                     /locus_tag="LGM24_RS29770"
                     /old_locus_tag="LGM24_29770"
     CDS             complement(3079..3402)
                     /locus_tag="LGM24_RS29770"
                     /old_locus_tag="LGM24_29770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_163063187.1"
                     /translation="MLTMWVTEGEHRRLLERCDGRPLAAWMRQTCLDEKPARTGKLPSL
                     SPALLRQLAGMGNNLNQIARRVNAGGGTGHDRVQIVAALMAIDAGLERLRHAVLEKGTD
                     DDR"
                     /name="MobC family plasmid mobilization relaxosome protein"
     Typing          complement(3518..3691)
                     /name="MOBP"
                     /typing="oriT"
                     /tool="MOB-suite"
                     /identity="97.714"
                     /coverage="100.0"
                     /evalue="1.2e-79"
     Typing          2..513
                     /name="rep_cluster_2335"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="93.567"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(1597..3089)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.8"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3086..3402)
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.453"
                     /coverage="99.0"
                     /evalue="1.41e-59"
ORIGIN
        1 tcgttcgact gcggcgagcg gaaatggctt acgaacaggg cggaaatttc ctggaagatg
       61 ccaggaagaa acttaacagg gaagcgataa ggccacgcca gagccgtttt tccataggct
      121 ccgcccccct gacaagcatc acgaaatctg acgctcaaat cagtggtggc gaaacccgac
      181 aggacttaaa gataccaggc gtttccccct ggtggctccc tcgtgcgctc tcctgttcct
      241 gcctttcggt ttaccggtgt cactccgctg ttatggccgc gtttatctca ttccacgcct
      301 gacactcggt tccgggaagg cagttcgctc caagctggac tgtatgcacg aaccccccgt
      361 tcagtccgac tgctgcgcct gttccggtaa ctatcgactt gagtccaagc cggaaagaca
      421 cgacaaagcg ccactggcag cagccactgg taagagggtg tacaagagat tactgatgca
      481 gagttcttga agtcagagcc aaactacggc taaactggaa ggacgaattt ggtggcagcg
      541 gtcttgtaca ccctgttacc acggttcaga agttccccaa cttactgaac cttcgaaaaa
      601 ccacctcccc aggtggtttt ttcgttttca gagcaagaga ttacgacgca acggaaagga
      661 tctcaagagg ttcatcttcg atgaccgaca gacatcaaag taaaaccgat accaaacaac
      721 agtaccagtc aaaaactcac accgacaaaa ccctttgaaa ataaagcata aaaaatcgta
      781 tagttggatg atgcacaatc acccaacaaa ccagcataac catttgaata aatagaaaaa
      841 taaaaatata ggatattaga gtaatgaaat acatttgctt tgcagcagta atgatttccc
      901 ttacaggatg cgttaattca agtggcgtat tacccatggg gccagatact tattccatat
      961 ctgttcatgc tgcacctgta aggggacagg ctggagcaaa aaaagctgcg ttaacagaag
     1021 cgaaccaata ttgccttaat caaggcaaga ccatccttgt gaagaacatg gatcactcta
     1081 cgtttagctc gctcccaggg gatgatataa gcatcatatt ccaatgctta tcggttggcg
     1141 atagtggatt acagcgacct aattatcaaa gtcctgctga tgtaaacgtc aacgttaatt
     1201 aggcaccatt taccataaca tccgttgggc gcaccgctga aacggggttg gctgcgtttc
     1261 cggtgccaga cgcgagaaag tggcgacaga cataacgaaa aaacaccaga aacgcagccg
     1321 ctgaatatta cactggtgca tagtcatgca gccagatgca tggatgttaa agtcacacta
     1381 aaatccgcgc caatgcctta aaaaccgaca aatgcttctc cagccagtcc cacagctccg
     1441 ccgccagata caggcagtta agcaatgatt caataagatt taacagcttc atttcccgct
     1501 cctgaataag tcgtgacctt tttgatcacg aacctattta gcgggagaaa taagagcccc
     1561 tcttcgtgcg ggttgtcagc tgcctggatg gtttaaagtg tcggcccgcg gtaggttttc
     1621 tgcggctggc gctccagctc cagtgttttc tgatactcat tctccctctg ccggagctcg
     1681 atggcattca gttcccggac aagcggcgca gctgctcgct taaactcagc acctgcgtgc
     1741 tcaagtccga cacgcgctcg ttcagcaccg cgttctcccg ttgcataagc ccacacatgc
     1801 tccgccattc cgcgaaggcg ctctcccatt cgtccagcct ttgcgagtag tcctgctgta
     1861 gctgctctaa tgcgctcagc aactgctttt ccagctctgt cattttccgc tactcctgtt
     1921 tcaggcggat ccatgtcgtc ccgtcgtagg ggatggcttc ggtttgcatc ccgggcggca
     1981 ggataagaaa cctccggccg tcgcggctct cctgatagcg cactccccac gtccgggcag
     2041 tcattttcgc cagggtatct tcctgctgac gaagatgcgt gtaattgtcg gttatctgct
     2101 gcccctgcca ccacagtatg ctcccgctcg tgccgatcag cagcgctgac accatcagga
     2161 tggtgaacca tgtccgcccg gcagtccgca gtacgctccg ccggtttttc tccatcgcct
     2221 cgctcatact ctgctcgtgt gcgaggatgg cgtcgctgat tctcctcgcg cttgcgctca
     2281 gttccgctct gacagactgc tcgtgctcgc tgaatgcggc tttcagcatc tcgccggtac
     2341 tctgcttctg cgctttcgat tgctgctcta agtccttcgc cagcgttaaa agactgctca
     2401 tagatggctc ccttcagtcg gatgtttcgc cccccgtccg ggtcggcaat gctgatactg
     2461 cttttcgtgg tgcgcaccac ctcaaaacct gaccgttcca gcgcctcagt gacgtcctga
     2521 cggtttttca gctccccgga cgaagcaagg gtcagcagtc cgcgcgtgat ggcttcagtg
     2581 gcctcctgct tcgttttcgg cagtccggac ggcgtgacca gcgcccggcg gttttccggc
     2641 gcgttcgggt catgcagccc cagtctgccg ttcacgatgg tctgccaagc atcgatgcgc
     2701 ggccggtcgg cgcggtcgta gtacggctgg agccgcctgc cggtcagcag ttcggtattg
     2761 gggatcagaa agttcagctc cagacgcccc ttgtccgcgt gctccaccca cagaatgctg
     2821 tactgatctt tatcgagtcc gggcatcaga acccgctcga agctggccat cagtttttcc
     2881 cgctggccgg gaggcagatc ggcctccgca aacgacagta cgccggaggt gtatttcttc
     2941 acgtaaggcg acgcgtcaat cagctcccgg acttcttccg gcttcccctg caggacgctg
     3001 gcgccttcac gctgccggtc tttacccagc agatagtcca ccggcccggc gccaccgccg
     3061 cgaccgcgtg gatgaaattt aacgatcatc gtccgttcct ttctcaagaa cggcgtgccg
     3121 cagccgctca agcccggcat cgatggccat cagcgccgcc acaatctgta ccctgtcatg
     3181 gccggtgccg ccgccggcgt taacacggcg ggctatctgg ttcaggttat tgcccatacc
     3241 ggcaagctgg cgcagcagcg ccggcgagag tgaggggagc tttccggtgc gcgcaggctt
     3301 ttcgtccagg caggtctgcc gcatccacgc cgccagcggc ctgccgtcgc agcgctcaag
     3361 cagccgccgg tgctcgccct ccgtcaccca catggtgagc atcttgttcc gtttctctga
     3421 caaaccggcc tcctgataac gccgggacgg gcgggaaagc ctttcccggt cggcgcgggg
     3481 ttggacaagc cgcaggcgcg tcagtggctt ttggcgggtt tcggggcgca gccctgaacc
     3541 agtcacgtag cgctagcgga gtgtatactg gcttactatg ttggcacgaa tgcggatgtc
     3601 agtgaagtgc ttcatgtggc aggaggaaaa aggcggcacc ggtgcgtcag cagaatatgt
     3661 gatacaggat atattccgct tcctcgctca ctgactcgct acgctcgg
//
