LOCUS       NZ_CP084799             3844 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain U41 plasmid
            pKqq_U41_10, complete sequence.
ACCESSION   NZ_CP084799
VERSION     NZ_CP084799.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024804
            Assembly: GCF_020525725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3844)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3844)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name :: Klebsiella quasipneumoniae subsp. quasipneumoniae
            U41
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,840
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 73 of 207
            Pseudo Genes (incomplete)         :: 139 of 207
            Pseudo Genes (internal stop)      :: 38 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084799.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3844
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="U41"
                     /host="Environment"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKqq_U41_10"
                     /geo_loc_name="Germany"
                     /collection_date="1990"
     gene            join(3450..3844,1..1009)
                     /locus_tag="LGM24_RS29740"
                     /old_locus_tag="LGM24_29740"
     CDS             join(3450..3844,1..1009)
                     /locus_tag="LGM24_RS29740"
                     /old_locus_tag="LGM24_29740"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_127713719.1"
                     /translation="MSEKSIEEMLAEKQIKEFNESKFGQFFNNKKLKEQELTYLNNKAL
                     DIVNEEDLFEIMSLIPQPFITDENKNVDVLSLIYNNFLEHTEIPLTLPIFSFLSFLSAF
                     CVKNEITYTIPLSDEKSPLDTWITILAPSGSGKSFSNSQMKKVIPTGIDGKKVIEPNFV
                     KPNGPAKFIEELSNLPTTKNGENQFGFWIEDEVSQMFKQVEQIGSPLSEIKEYLLKAYD
                     HEKLTRKSKKESIETKGIILTLLFINTFESYANNISNESMTDGLMRRFNLIYTEKDIER
                     DFTDYYLYNLKKIRDDRLQDEIADLFFSIKPDQNFTFSDNCFKLYGKYFKIFWHRKYKN
                     ILTDKENFYRTYMMQSWKFAVFYHLILKREGTEIQGDCLIYGMKISLLFANSMKKFFDF
                     KIRKNVPDKKIEKIKNDLTKYIVFLENNPEATLRDFCRKYNIRKIDANSILKSLQCANI
                     QHKLIEELE"
                     /name="hypothetical protein"
     gene            1291..1638
                     /gene="mobC"
                     /locus_tag="LGM24_RS29745"
                     /old_locus_tag="LGM24_29745"
     CDS             1291..1638
                     /gene="mobC"
                     /locus_tag="LGM24_RS29745"
                     /old_locus_tag="LGM24_29745"
                     /inference="COORDINATES: protein motif:HMM:NF017524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="WP_048982277.1"
                     /translation="MSYRERYQRKNFISLCLSDEELSEIENIADRLNMKRAAAAREILV
                     TNSKRLKSQIKKNDNSEILFLYSKISNNINQIAKKMNTNLDKFLSGNGEEFSLLIEEIF
                     EDLERLKNNDT"
                     /name="mobC"
     gene            1628..3052
                     /locus_tag="LGM24_RS29750"
                     /old_locus_tag="LGM24_29750"
     CDS             1628..3052
                     /locus_tag="LGM24_RS29750"
                     /old_locus_tag="LGM24_29750"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_136070838.1"
                     /translation="MIHKHIGGKKNQRSVKNSLDYLLRTKKPEEQKFVEVLSQTNRSDI
                     ENFNEYIKDKNFKNPFITGVLSFEEKDIDPELKTKIMNDFEDMMFSGIEPENRPPVIWI
                     QHKDKNRLELNYTTFNSLMNKKHFQCYYHLSDKKLFNSFCEKINYENNISSVLDVQEHK
                     ARNTLVNTLNNKIPEEKKAVVEKLQEEILSKIITEEINNREELISFIQSKNIIINRVRE
                     NAISIKFDKADKPISLKGDIYEQNRDYKTYRTESTIDHRADQQYVENQLRHHRENFDTE
                     LKKRHSRNRTRFKPTQSKDQKNVEYRVKKANDSKNVRSEIPDNNNVPVRNEPDIDFFHN
                     NKTIEDVSNEQNFTNDKTKPREEEPAKFDLSREIETANTQQQFIRSRVEEVRRNQQQFR
                     KSLDRTQERSGVCRRSLHSIIHNIRRYVRTFSIHTRQNERRQKIEHRQKIFLEKLKTQQ
                     EKNNQRSVVRSRFKPY"
                     /name="hypothetical protein"
ORIGIN
        1 atcaggtagc ggaaaatcat tctcaaattc acaaatgaaa aaggttattc ctactggaat
       61 agatggaaaa aaagttattg aaccgaactt tgtaaaacct aacggtcccg caaaatttat
      121 cgaagaactt tcaaatttac ccacaacaaa aaatggcgaa aatcaattcg gattttggat
      181 tgaagatgaa gtttcacaaa tgttcaaaca agtagaacaa attggatctc cgctctctga
      241 aattaaagaa tatttattaa aagcatatga ccatgaaaaa ttaaccagaa aaagcaaaaa
      301 agaatcaata gaaaccaaag gcattatttt aactttatta tttataaata catttgaaag
      361 ttatgcgaat aacatttcaa atgaatctat gacagacgga ttaatgcgta gatttaattt
      421 aatatacaca gaaaaagata ttgaacgaga ttttactgat tattatttat ataatttaaa
      481 gaaaataaga gatgatagat tacaagatga gattgctgat ttgttttttt ctataaaacc
      541 agatcaaaat ttcacctttt ctgataattg ttttaaatta tatggcaagt attttaaaat
      601 tttttggcat agaaaatata aaaatatact cacagataaa gaaaattttt atagaactta
      661 tatgatgcaa agctggaagt ttgctgtatt ttatcattta attttaaaaa gagaaggaac
      721 tgaaattcag ggtgattgtt taatatatgg gatgaaaata tcattacttt ttgctaacag
      781 tatgaaaaag ttttttgact ttaaaataag aaaaaacgtt ccagataaaa aaatagaaaa
      841 aataaaaaat gatttaacaa aatatattgt ttttttagaa aataacccag aagctacatt
      901 gagagatttt tgcagaaaat ataacatcag aaaaatagat gctaattcta tattaaaaag
      961 tcttcaatgt gctaatattc aacacaaact tattgaggaa ctggagtaat taacaagcga
     1021 attttaaatt gtgccatatt caaatatatt ttcgcttgtt caacccctca aaaataataa
     1081 agccctgacg ggctttttta ttttctcacg gttttataaa aggtcaaatt atattcagtt
     1141 ttgcccttga cttaatgttt taatttgtta tctatgtaat agatagcaag ttagagtaat
     1201 acttcgtatt cctttacttg tgaggggttc ccccttcaat cccctaaaaa ccaaatcaaa
     1261 atcaaaacct taaacccaaa ggagttaaat ttgagttacc gtgaacgata ccagagaaaa
     1321 aattttatct cattgtgttt gagtgatgaa gaattatcag agattgaaaa tattgctgat
     1381 aggttaaata tgaaacgagc agccgcagct cgtgaaatat tagtcacaaa ctcaaagaga
     1441 ctaaaatcac aaataaagaa aaatgataat tctgaaatat tgtttttata ttctaaaatc
     1501 tcaaacaata taaatcagat cgcaaaaaag atgaacacta atcttgataa attcttatca
     1561 ggaaacggag aagaattttc actactcatt gaagaaatat tcgaagattt agagaggtta
     1621 aaaaataatg atacataaac atatcggagg aaagaaaaat cagcgaagtg ttaaaaattc
     1681 acttgattat ttattaagaa ctaaaaaacc agaagaacag aaatttgttg aagttttatc
     1741 tcaaacaaac agatctgata ttgaaaattt caacgaatac ataaaagaca aaaactttaa
     1801 aaaccccttc ataactggtg ttttatcttt tgaagaaaaa gacatcgatc cagaattaaa
     1861 aacaaaaata atgaatgatt ttgaagatat gatgttttca ggaatagaac cagaaaacag
     1921 acctccggtc atttggatac aacacaaaga taaaaacaga cttgaattaa actatacaac
     1981 ttttaattcc ctaatgaata aaaaacattt tcaatgttat tatcatttgt cagataaaaa
     2041 attattcaac tcattttgcg aaaaaataaa ctatgaaaat aatatcagtt ctgttttgga
     2101 tgttcaggaa cataaagcaa gaaataccct cgttaacact ttaaataata aaataccaga
     2161 agaaaaaaag gcagttgttg agaaattaca agaagagatt ttatcgaaaa taatcacaga
     2221 agaaataaac aacagagaag aattaatatc atttattcaa tctaaaaaca taataataaa
     2281 cagggttaga gaaaatgcta tatcaataaa atttgataag gctgataagc ctatcagctt
     2341 aaaaggagat atatatgaac agaacagaga ttacaaaact tatagaacag aatcaacaat
     2401 cgatcacaga gcagatcagc agtatgtcga aaaccaactc agacaccata gagaaaattt
     2461 tgacacagag cttaaaaaaa gacacagcag aaatagaact cgttttaagc caacacagag
     2521 caaagatcag aaaaatgttg aatacagagt taaaaaagca aatgacagta aaaatgttag
     2581 atctgaaata ccagacaata ataatgttcc tgttaggaat gaacctgata ttgatttttt
     2641 tcataataat aaaactatcg aggatgtaag caatgagcaa aatttcacaa atgataaaac
     2701 aaaaccaaga gaagaagaac cagcaaaatt tgatctatca agagaaattg aaacagctaa
     2761 tacacaacaa caattcattc gttcaagagt tgaagaggtt agaagaaatc aacagcaatt
     2821 cagaaagtcg cttgatagaa ctcaggaacg atctggagta tgtagaagat ctctacattc
     2881 aattattcac aatattagaa gatacgtcag aacgttttca atccatacaa gacagaatga
     2941 aagaagacaa aaaatcgaac acagacaaaa aatcttcctt gaaaagctca aaacccagca
     3001 agaaaaaaac aatcaaagaa gtgtcgtcag aagtcgattt aaaccgtatt agaaccgttc
     3061 tgaacactct tctaacccac tatgacaaaa ccttaaccgt gaacaagtga aagccccaca
     3121 tagtggggtt tttgcttgtg aggggttggc ctgccggcga ggcggttttg tcgtttgttt
     3181 ttttattttt tatgacaggt aaaaaataaa tatgtttcaa aatatttcaa aaactccaag
     3241 taatttgatt ttgtcaaaac tttgtcaaag atttgtcaaa accaaaaaaa atggtttttc
     3301 ctttaaaaac aaaggcttta ttgatttgtc aaagtgtcat agtgtcaaaa agaagcgcga
     3361 aaaaaaagtc atattttttt taaaaaatat ttgacacatt taaaaatgta ttatatatat
     3421 tttatataat caaaatagga gaattaatta tgagtgaaaa atcaattgaa gaaatgttag
     3481 ctgaaaagca aataaaagaa tttaacgaat caaaatttgg tcagtttttt aataataaaa
     3541 aactaaaaga acaagaacta acatatctta acaataaagc attagacatc gtaaatgaag
     3601 aagacttatt cgaaataatg tcattaatac ctcaaccttt tattactgat gaaaataaaa
     3661 atgtagatgt attaagtctt atatataata attttttaga acatacagaa ataccattaa
     3721 cacttcccat cttttcgttt ttaagttttt taagtgcttt ttgtgttaaa aatgaaatca
     3781 catatacaat tccattgagc gacgaaaaat cgccgcttga tacttggatc acaattttag
     3841 cccc
//
