LOCUS       NZ_CP084794            12171 bp    DNA     circular CON 26-JAN-2024
DEFINITION  Klebsiella quasipneumoniae subsp. quasipneumoniae strain U41 plasmid
            pKqq_U41_5, complete sequence.
ACCESSION   NZ_CP084794
VERSION     NZ_CP084794.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22024804
            Assembly: GCF_020525725.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella quasipneumoniae subsp. quasipneumoniae
  ORGANISM  Klebsiella quasipneumoniae subsp. quasipneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 12171)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     A curated collection of Klebsiella metabolic models reveals variable
            substrate usage and gene essentiality
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12171)
  AUTHORS   Hawkey,J., Vezina,B., Monk,J.M., Judd,L.M., Harshegyi,T., Brisse,S.,
            Holt,K.E. and Wyres,K.L.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-OCT-2021) Infectious Diseases, Monash University, 85
            Commercial Rd, Melbourne, VIC 3004, Australia
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 01-JAN-2021
            Assembly Method :: Trycycler; Medaka; Pilon v. 0.3.3; 1.3.3; 1.23
            Assembly Name :: Klebsiella quasipneumoniae subsp. quasipneumoniae
            U41
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50x
            Sequencing Technology :: Minion (Oxford Nanopore) and NextSeq
            500/550 (Illumina)
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/24/2024 14:21:43
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,967
            CDSs (total)                      :: 5,840
            Genes (coding)                    :: 5,633
            CDSs (with protein)               :: 5,633
            Genes (RNA)                       :: 127
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 90
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 207
            CDSs (without protein)            :: 207
            Pseudo Genes (ambiguous residues) :: 0 of 207
            Pseudo Genes (frameshifted)       :: 73 of 207
            Pseudo Genes (incomplete)         :: 139 of 207
            Pseudo Genes (internal stop)      :: 38 of 207
            Pseudo Genes (multiple problems)  :: 37 of 207
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084794.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..12171
                     /organism="Klebsiella quasipneumoniae subsp.
                     quasipneumoniae"
                     /mol_type="genomic DNA"
                     /strain="U41"
                     /host="Environment"
                     /sub_species="quasipneumoniae"
                     /db_xref="taxon:1667327"
                     /plasmid="pKqq_U41_5"
                     /geo_loc_name="Germany"
                     /collection_date="1990"
     gene            1..897
                     /locus_tag="LGM24_RS29545"
                     /old_locus_tag="LGM24_29545"
     CDS             1..897
                     /locus_tag="LGM24_RS29545"
                     /old_locus_tag="LGM24_29545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197749.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="WP_004197749.1"
                     /translation="MYKKSVDLSLESVLVEIDENKKLMPSRNATVQPVALMRLGLFLPS
                     LKRKGKDTENISIDASNELQSLELARAEGYTDIRITGPRLSMETDFKVWVGIILAFSKY
                     GLNSSTIELPFSEFATLCGFSSKDKDKGLRTRLADSLIRLRSTTIKLASEKDRNGVVSG
                     LLSRGKWDEKDDIMELTADESLWELYQFDRQVLLQMFIIRQLANKGTAQALYTFIESLP
                     ERPIPLSFARIKRRLMLTSPNNQQNRVINKAIDELKAVGYLDGDVVKKDGEWHLIITRR
                     TPRPDIKAMSEALKDGE"
                     /name="RepB family plasmid replication initiator protein"
     gene            1219..2424
                     /locus_tag="LGM24_RS29550"
                     /old_locus_tag="LGM24_29550"
     CDS             1219..2424
                     /locus_tag="LGM24_RS29550"
                     /old_locus_tag="LGM24_29550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="WP_004152349.1"
                     /translation="MKRDYGGVGSIARRAGILLESMSRDIEDQRKEFNLTEYFQTFTRN
                     AVAKLPKLSRRIVEQAIKEMEDAGYDFNKKRVGNVEQYALTVQNVIDIYAHRKIPKYRE
                     IHQSPYVIFVVNLKGGVSKTVSTVTLAHALRVHQDLLRHDLRILVIDLDPQASSTMFLD
                     HTHSIGSILETAAQAMLNDVDAETLRNEVIRPTIIPGVDVIPASIDDGFVASAWKELVS
                     EHLPGVNQYEVLRKIIIDRVADDYDFILIDTGPHLDPFLLNGLAASDLILTPTPPAQVD
                     FHSTLKYLTRLPEMLETLEEEGIEPRLAASIGFMSKMTGKPDHLVSHSLAREVYTSSIL
                     DSSLPRLDGFERCGETFDTIISANPASYPGSNDALKKARTEAEHFTKAVFDRIEFLRSQ
                     SA"
                     /name="AAA family ATPase"
     gene            2421..3395
                     /locus_tag="LGM24_RS29555"
                     /old_locus_tag="LGM24_29555"
     CDS             2421..3395
                     /locus_tag="LGM24_RS29555"
                     /old_locus_tag="LGM24_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="WP_004197750.1"
                     /translation="MKKKIVARGRNILGSDSSDFIRSLETKEAEKVFTLKSGNQAKFTL
                     STILNGDIEEKTFVDPAVNGRDQRYVTPESVSDITRTINLQQFFPAIGRVIDGCIEVLD
                     GSRRRAACIFSGAKFEILVTEDEISLEDARQLAKDIQTAREHTLREIGQRYQFMYANGM
                     SKDEIARVEDVSPASVSRAFQAASVPAEMVELFPVINELSLADYQLLLKISEDLDSKGV
                     PLSDLLGKVQADISAAKVESISKSLIMDSFKRHSKQLKPAPVKTVQTEKLREFEDKKQF
                     ARKKTDPSKRLVTYEFARLPASVQAELDEAIRLVMGNMQSFEK"
                     /name="ParB family protein"
     gene            3944..4969
                     /locus_tag="LGM24_RS29560"
                     /old_locus_tag="LGM24_29560"
     CDS             3944..4969
                     /locus_tag="LGM24_RS29560"
                     /old_locus_tag="LGM24_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015632466.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="WP_001101446.1"
                     /translation="MNVKTIGIDLAKNVFQIHGVDEHGKRLFNKQLRRAQMASFFANIP
                     PCLIGMEACASAHFWANKLISMGHNVKLMAPQFVKPYVKTNKHDAADAEAICEAVTRPN
                     MRFVPVKTAEQQAVLALHRSRQSFIKQRTAQANQIRGLLAEFGIVVPRGIQQLQRRLPE
                     LVEDADNPLPVLFRTQLSLLQHHMAYLFDVIATLDKQIEQCYRQNALCQRIGKIPGIGP
                     VTASALIATIGKANNFENGRQLAAWLGLVPRQHSSGGKQVLLGISKRGDTYLRTLLIHG
                     ARAVLQSAKHKQDAVSSWANQLMARRNNNIASVALANKNARTVWALLAKEREYCAPIIS
                     A"
                     /name="IS110 family transposase"
     gene            complement(<5240..>5374)
                     /locus_tag="LGM24_RS29565"
                     /old_locus_tag="LGM24_29565"
                     /pseudo=""
     CDS             complement(<5240..>5374)
                     /locus_tag="LGM24_RS29565"
                     /old_locus_tag="LGM24_29565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490546.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5623..>5825)
                     /locus_tag="LGM24_RS29570"
                     /old_locus_tag="LGM24_29570"
                     /pseudo=""
     CDS             complement(5623..>5825)
                     /locus_tag="LGM24_RS29570"
                     /old_locus_tag="LGM24_29570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="frameshifted; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /name="hypothetical protein"
     gene            complement(5988..6218)
                     /locus_tag="LGM24_RS29575"
                     /old_locus_tag="LGM24_29575"
     CDS             complement(5988..6218)
                     /locus_tag="LGM24_RS29575"
                     /old_locus_tag="LGM24_29575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_009309993.1"
                     /translation="MSPGFRNVLRLARIPVDSSEPVRRKFGSRLIGIVTVCLLPLLVFD
                     VPQWVFILSQIIWGAGVYIGLSVAGDKSLNR"
                     /name="hypothetical protein"
     gene            complement(6282..6953)
                     /locus_tag="LGM24_RS29580"
                     /old_locus_tag="LGM24_29580"
     CDS             complement(6282..6953)
                     /locus_tag="LGM24_RS29580"
                     /old_locus_tag="LGM24_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="WP_032431399.1"
                     /translation="MPDGQYSFYLHSDDRTDIAAMATISTISQPLRGEFIRTAATAGAV
                     VYQVDARLPALIPVFFAGQLSAVRLYAVMALVSGTWSSLTGLPDEPENGGAALLVSPET
                     EYQRRRYTLTLQNGRSGERVESVLTEASSRLRGDLLRNLIITGLALHTTAPELPRLLAS
                     MPVPPTSVSELQALVQQMAGTADVSRAAAAVPDTPATSDVPVSGADTREIKKNMRRAFG
                     D"
                     /name="plasmid partitioning/stability family protein"
     gene            complement(6956..7927)
                     /gene="parM"
                     /locus_tag="LGM24_RS29585"
                     /old_locus_tag="LGM24_29585"
     CDS             complement(6956..7927)
                     /gene="parM"
                     /locus_tag="LGM24_RS29585"
                     /old_locus_tag="LGM24_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="WP_001568038.1"
                     /translation="MNIYCDDGSTNVKLAWFEGDELQTRVSANSFRHGWKVAEFSAATF
                     NYQVGTLKYTWDSVSRDAIPTTNVEYQYGDLNLLAVHHALLNSGLEPQPVSLTVTLPLS
                     EYYDGDCQRNEENIRRKRENLMRELVLNKGRAFTVTDVKVMPESLPAAFSRLAELKPGP
                     AETTLIIDLGGTTLDAGVIVGQFDDISAVHGNPSVGVSQVTRAAAGALRAADSETSALI
                     ADTIIRNRNDRQYLQRVINDAGKIDEVRNKITEAITSLGARVTSELTAFRNVNRVFLVG
                     GGASLIEEAIRQAWPLAPDRIEVIGDPQMALAREIALYNKED"
                     /name="parM"
     gene            complement(8176..9663)
                     /gene="ltrA"
                     /locus_tag="LGM24_RS29590"
                     /old_locus_tag="LGM24_29590"
     CDS             complement(8176..9663)
                     /gene="ltrA"
                     /locus_tag="LGM24_RS29590"
                     /old_locus_tag="LGM24_29590"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_023332914.1"
                     /translation="MMYGEEKSDSLIVAANLANNPQGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTAEALENAFLSLSRKAAAGVDGVRWKD
                     YAENLKVNIADLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHNALDALATGLVRTNVNWVLDADISQFFDKVSHEWLIR
                     FIEHRIGDQRVIRLIRKWLTAGTSEEGEWRASEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRHATGNVVMVRYADDIVIGFDKRIDAQCFRIAMQRRLKEFGLTVHPKKTRLMEF
                     GRFAAENRASRGKGKPETFNFLGFTHISGKDRSGRFMLIRKTRRDRMTATLKAIKDGLR
                     KRWHYSIPEQGKWLRRVVQGYLNYHSVPGNYPMMRKFRIYVTDLWRRALRRRSQQDDTT
                     WTKANRLAAVWLPKVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            10069..10500
                     /gene="umuD"
                     /locus_tag="LGM24_RS29595"
                     /old_locus_tag="LGM24_29595"
     CDS             10069..10500
                     /gene="umuD"
                     /locus_tag="LGM24_RS29595"
                     /old_locus_tag="LGM24_29595"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229772.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="WP_004197746.1"
                     /translation="MKLQFFSTGKELPPQAHPLFADLASCGFPSPAADYVESDLDLHDY
                     CIRHPSATYYLRASGDSMADGSLYNGDLLVVDCAEKPRHGDIVVASVQGEFTVKRLLLT
                     PRLTLQPMNAAWSPIYPDPDDLDIFGVVTHIIHRPREMY"
                     /name="umuD"
     gene            10500..11771
                     /locus_tag="LGM24_RS29600"
                     /old_locus_tag="LGM24_29600"
     CDS             10500..11771
                     /locus_tag="LGM24_RS29600"
                     /old_locus_tag="LGM24_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="WP_004197744.1"
                     /translation="MFALADVNSFYASCERVFRPDLKGKPVVVLSNNDGCVIARSAEAK
                     PWIKMGTPWFQMKNERYPEKIYAFSSNYELYASMSARVMSCLEELAPGVEQYSIDEMFL
                     DLTGVEHCMDLEDFGRQLRQHVYDCTRLTIGVGAGPTKTLAKSAQWASKEWKQFRGVLA
                     LTRGNPQRTRKLLSLQPVEEIWGVGNRIARKLNVLGIKTALDLALTNPAFIRKNFSVVL
                     ERTVRELNGESCMSLEEAPPTKQQIVCSRSFGEKVTEYDSLRQAVCQYAERASEKLRKE
                     RQYCRHISVFIKTSPFAVKEPYYGNVATEKLLTPTQDTRDIIAAATTALERIWKDGHRY
                     AKAGVMLNDFTGSGVSQLQLFDERPPRPHSAELMKVLDGINHSGLGQVWFAGRGIAPAW
                     QMKRDMLSPAYTTRWKDIPIVRIG"
                     /name="Y-family DNA polymerase"
     Typing          2..897
                     /name="IncFIB"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="99.666"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgtataaga aaagtgtaga tctatcactt gaatcggttc tcgtagaaat cgatgaaaat
       61 aagaagctca tgccttccag aaacgctact gtccagcctg tagctttgat gcgcttaggt
      121 ctgtttctac cttccttaaa acgaaaagga aaggatacag aaaatatcag tattgatgcg
      181 agtaacgaac ttcaaagctt agagttggca agagccgaag gttatacaga catccgtatt
      241 accggcccac gactttcgat ggagactgac ttcaaggtgt gggttggcat cattctcgcg
      301 ttttcgaaat atggcttaaa cagcagcacc attgaacttc cattcagcga gtttgctaca
      361 ttatgcgggt ttagctcaaa ggacaaagat aaaggactca gaacgcgctt ggccgactct
      421 cttattcgtc ttcgcagcac aactatcaag cttgcctcag aaaaggatcg gaatggtgtt
      481 gtatctggtc tgttaagtcg tggtaagtgg gacgaaaaag acgacatcat ggaacttacc
      541 gccgatgagt ctctttggga gctttatcaa ttcgaccgtc aggtgcttct gcagatgttt
      601 ataatccggc aacttgcgaa caaagggaca gcacaggccc tatacacttt cattgagagc
      661 cttcctgagc gaccaatacc actatcgttc gcgcgtatta aacgtcgcct aatgcttacc
      721 tcacccaata accagcagaa tcgagtgatt aacaaggcta tagacgagct taaagcggtc
      781 ggttatttag atggcgatgt cgttaaaaag gatggtgaat ggcacttaat cataacccgt
      841 cgcacgccta ggccagacat taaggctatg tctgaagcct taaaagatgg agagtaaaag
      901 cgcctgactg tgtttgtaaa gcggatatgt gccgcagtga ctgcgagtgt ttgctgtttt
      961 cccggtaaca cagcatcagc atccgtttta taaacacatc tattccctct catattgttt
     1021 ccgctttgta aacacacctg tttgctcact tccgaaccga tttataaaca catgtatcag
     1081 cgttcagcct ttgagtcact cgctgaacgt gcaacaaaaa caccaatatc aataatttct
     1141 tgcacgtcat ggtatttgtt gtacactccg agttgtacaa tcaatgtcct ctacattgaa
     1201 aacatcatgg agaacagaat gaaacgagat tacggtggag taggctcgat agcgcgacga
     1261 gctggcatat tacttgagtc aatgagccgg gacattgaag atcaacgcaa agagtttaac
     1321 cttacagaat acttccagac ctttacccgg aacgcagtag ccaaattacc caagctaagc
     1381 cgtcgcatcg tggagcaggc cattaaagaa atggaagatg caggctacga tttcaacaag
     1441 aaacgcgtcg gaaatgttga gcaatatgct cttacagtac agaatgttat tgatatttac
     1501 gcccaccgca agatcccgaa gtatcgtgaa atccatcaat cgccttacgt tattttcgtc
     1561 gttaacttga aaggcggcgt atccaaaacc gtatcaactg taacgctggc gcacgcgttg
     1621 cgagtgcatc aggatttatt gcgtcacgat ctgcgtattc tggttattga ccttgatccg
     1681 caggcatcca gcaccatgtt cctcgatcac acgcacagta ttggttccat tcttgaaacc
     1741 gccgcgcaag cgatgctaaa cgatgtcgat gctgaaaccc tgcgtaacga agtgatacgg
     1801 ccaaccatca ttccaggtgt tgatgttatt ccggcttcta tcgatgatgg ttttgtagcc
     1861 agcgcatgga aggagctggt tagcgagcac ctgccgggcg taaaccagta cgaagtgctt
     1921 cgcaaaatca ttatcgatcg tgtggctgat gactacgatt tcatcctgat tgatacaggt
     1981 ccgcacctcg atccattttt actaaacggt ttggcggcaa gtgaccttat tctcacccct
     2041 actccgccgg cgcaagtcga tttccattcc acgttgaaat accttacccg cttgcctgaa
     2101 atgctggaaa cgctggaaga agaaggcatt gaaccgcgtc tggctgccag tattggtttt
     2161 atgtcgaaaa tgactggcaa gcccgatcac ctggtttcac atagccttgc tcgtgaagtc
     2221 tacacaagta gcatactgga ctcttccctg ccacgcctgg atggctttga acgttgcgga
     2281 gaaacgtttg atacgattat tagtgctaac ccagcatctt atcccggtag caacgacgcg
     2341 ttaaaaaaag cgcgtacaga agcagagcat tttacgaagg ctgtgtttga ccgtattgaa
     2401 ttcctgagga gccaatcagc gtgaagaaaa aaatagtagc tcgcgggcga aacattttag
     2461 gtagtgatag ctcggatttt atccgtagcc tcgaaaccaa agaagcagag aaggtattca
     2521 cccttaagtc cggtaatcag gccaagttca ccctctcaac cattttgaat ggtgatattg
     2581 aagagaagac atttgtcgat ccagctgtta acggtcgcga ccaaaggtat gtaacccctg
     2641 agtcagtgag cgacataacg cgaactatca acctgcagca atttttccca gcaatcggcc
     2701 gagttattga cggttgtatt gaagtgttag atggttcccg gcgccgggcg gcatgtatct
     2761 tcagtggcgc gaaattcgaa attctggtta cagaagatga gataagtctt gaagatgctc
     2821 gtcagcttgc caaagacatt cagaccgcgc gtgagcatac gcttcgtgag ataggtcaaa
     2881 gatatcaatt tatgtacgca aatggcatgt ctaaggatga aattgcgcgc gtagaggacg
     2941 tctcaccggc cagcgtttcc cgcgcgtttc aagctgcttc tgtgccagct gaaatggtcg
     3001 agttattccc ggttatcaat gaactctccc ttgctgatta ccagctgctg ttaaaaattt
     3061 cagaggactt agatagcaaa ggtgtgcctc tgtctgattt gctcggaaaa gttcaggcag
     3121 acatcagtgc agcaaaagtt gaaagcataa gtaagtcgct aattatggac tcttttaaac
     3181 gccattcgaa gcagctgaag ccagcgccag tgaaaactgt acaaactgaa aaactacgtg
     3241 agtttgaaga caaaaagcag tttgcccgga agaaaaccga tccgtctaaa cgcttagtga
     3301 cctatgaatt tgcccgtctt cctgcttcag ttcaggctga gttagatgag gccatcaggc
     3361 tggttatggg caacatgcag tccttcgaaa aataaaaaaa gtgggaattt aatgtgtgac
     3421 tccactaaaa agttcccact acattttcag tcctaactat ctaaaaaact aggttttttg
     3481 tgatttcttt cagtatgaaa ttccctgtcg catcgcttga agttttataa agcacaatgg
     3541 cgtaattttt ggagaaacaa aatcaatgga acactcgaca atttctgagg ctctaattgg
     3601 tggtgttgtt tgcggcacta tcgcggccgg atttatctgg caattattca gaccttggaa
     3661 acttgaatgg ctctggttaa cctgttttat ccttacctca atcattgtat ttttctttgg
     3721 tccctctatg ccaaacccat tcatgatgta gaagtacaag gagatgtgcc atgaccgtga
     3781 ggctttatta caatgcagca gatagacgag cccaagcgag caatgaatgg catgctgagt
     3841 ggataagtaa acgtggcggc tttgttaatg gaatggtcgc ctcccatctc ttgtgccaca
     3901 gtgggacgat aaatcgtcac tcattgaacg gagcgctgct atcatgaacg ttaaaactat
     3961 tggaatcgat ttggcaaaaa acgttttcca gatccatggg gttgacgagc acggaaaacg
     4021 gttgttcaac aaacaactca gacgggcaca aatggcctcc ttttttgcca acatcccacc
     4081 ctgtttgatc ggcatggagg cctgtgcatc tgctcatttc tgggccaata aactgatatc
     4141 gatgggccat aatgtcaaac tgatggcccc tcagttcgtc aaaccctatg ttaaaaccaa
     4201 taagcatgat gctgcagacg ctgaagctat ttgtgaagcc gtcactcgac ctaacatgcg
     4261 gttcgtgccg gtcaaaaccg ctgagcagca agccgtattg gcacttcacc ggagtcgtca
     4321 gagcttcatc aaacagcgaa ccgcacaagc caatcaaatc agggggttat tggccgaatt
     4381 tggcattgtc gtcccccgag gtatccagca gctacagcga cgattacctg agctcgtgga
     4441 agatgcggat aacccgttac ccgtcctgtt tcgtacacag ctgagtctac tacagcacca
     4501 catggcgtac ctgttcgatg tcatcgctac actcgacaag cagattgagc agtgctatcg
     4561 gcaaaatgct ctctgccagc gtatcggcaa gatccctggt attggccctg ttaccgccag
     4621 cgcgctgatt gcgaccattg gtaaagccaa caatttcgag aatggccgac aactggctgc
     4681 ctggctcgga ttggttccac gtcagcactc cagtgggggt aaacaagtcc tgctcgggat
     4741 aagcaagcga ggtgatacct atttgaggac cttgcttatc catggtgcca gggcggtatt
     4801 gcagtcggcc aaacataaac aggatgccgt atcgagctgg gctaaccagc taatggcgcg
     4861 ccggaataac aacattgcct cggtagcatt ggctaacaag aatgcgcgga ctgtgtgggc
     4921 gctcctggcc aaagagcggg agtattgtgc accaataata agcgcttaag ttgcttaatc
     4981 agtaagacag aaacaacacc accgattgcc caggcaagca tgaagtgatg gcaagacagg
     5041 tcagaccgcg atggggaaaa cccggtttat tcaaggctcc tgaaagagcg ctttgttgat
     5101 agaggcctcc atcagcgtat tccatcaggg acagaggtat tttacatcac ctcggcaaag
     5161 tccggatcta tggctgcaat cgatgaccag taaagccacc acaacatatt tagcttggca
     5221 aacaggaggc gaccatctat gcatcgatgt cgccttccgt gagcgagccg tccagtgaaa
     5281 gaaaatcatc ggccagccag aacgtgccgc agcacagcgc gtcgtcgggg tactgcgaca
     5341 aacggtctat aaaggctttc acggtcatgc gtttttccat tctgtctccg ggcggtctcc
     5401 cgcccgcccc ctgttcagcc gccaaagcgt gcaatgttgt tgataatcca gtccgggcag
     5461 aggcgattgc tcgccacgca cccacgtttt gagttcatcg gcgtgatgta ttccactgaa
     5521 ggtcagccgg ttagcgcacc ggttcggcat atgcagatcc tgaatagtca gcgtgggcag
     5581 gaggcatcac cgcgataccg ttccctgccg cacgctgcct gttcatcgcg tccggtcacg
     5641 ggtcaggccg accgtggaat accgcagccg tccgtctttt ggacggcaag gatatgccgc
     5701 gaaagcggct tgaagggcgt tccttaaaag ggcgagggac tggcgcacac tctcaacgat
     5761 gccgggtcgg agcggaaaaa ccgcccgtcc cgtaccagac cggacgcgag acactggcag
     5821 cgggcggtcg gcttacgtta actccaggcg aacggggaat taccggcgta cctgtccaca
     5881 aatccggggc ttaaggctgt ggataacctg aaggcaaacg acggagaagc acgggagaac
     5941 agacacttat aagactgttc ttttttcaaa caaccagaaa atctgtatca gcggttcagg
     6001 gatttatccc cggccacaga caggccaata tagacgcccg ccccccagat gatctgcgac
     6061 aaaatgaaaa cccactgcgg aacgtcgaaa accagaagcg gaagcagaca taccgtcaca
     6121 atgccaatca gccggctgcc aaattttctg cgcaccggtt cactgctgtc gacgggaatg
     6181 cgggcgagac ggagaacatt tctgaatcct ggtgacatga gaacctccct ggggatgcca
     6241 gtcatttgaa atcggtatag tagtggatgt gattcgtact gttagtcacc gaatgcacgc
     6301 cgcatatttt tctttatttc ccgggtatcc gccccggaca ccggcacgtc agatgttgcc
     6361 ggcgtatccg gtactgcagc tgcagccctg ctcacgtcag cggtgccggc catctgctga
     6421 accagtgcct gcagctcact gaccgaggtg ggcggtactg gcatactggc cagcaggcgc
     6481 ggcagttccg gcgccgtcgt gtggagggca agaccggtga taatcaggtt tctcagcaga
     6541 tcgccacgca gacgggatga cgcttccgtc agcacacttt ccacccgttc cccactccgg
     6601 ccattctgaa gcgtcagggt gtaacgccgg cgctggtatt ctgtctccgg tgacacgagc
     6661 agagcagccc caccattttc gggctcatcc ggaaggccgg tgagtgagga ccaggttccg
     6721 gagaccaggg ccatcacggc gtaaaggcgg acagcgctca gctgcccggc aaagaacacc
     6781 gggatcagcg ccggtaaacg ggcatccacc tggtatacca ccgccccggc ggtggcggcg
     6841 gtacggatga actcaccgcg cagaggctgt gagatggtgc tgatggttgc catcgccgcg
     6901 atgtcggtgc gatcgtcgct gtgcagatag aaagaatact ggccgtccgg cacgtttagt
     6961 cctctttatt gtaaagggca atctccctgg ccagcgccat ttgcggatcg cctataacct
     7021 cgatacggtc aggagccaga ggccaggcct gacggatagc ctcttcaatg agggatgccc
     7081 ctcccccgac caggaatacc cggttaacgt tcctgaaggc cgtcagctca ctggtgacac
     7141 gcgcgcccag ggaggtgatt gcctctgtta ttttgttccg aacctcgtca attttaccgg
     7201 catcgttgat cacacgctgc agatactgac gatcattgcg gttgcggatg atagtgtcag
     7261 cgatgagggc gctggtttcg ctgtctgccg cccgcagcgc gcctgcagct gcgcgagtga
     7321 cctgtgacac gccgacagac ggattaccgt gcaccgcact gatatcatca aactggccga
     7381 caatcacacc ggcatcgagc gtggtgccac cgagatcgat aatcagggtg gtctcagcgg
     7441 ggccgggctt cagctccgcg agacgggaga acgccgccgg cagtgattcc ggcatcacct
     7501 tcacgtccgt tacggtaaat gctctgccct tattcagaac cagctcacgc atcaggtttt
     7561 cccgtttgcg tcgtatgttc tcctcgttcc gctggcagtc cccgtcgtaa tactcgctca
     7621 ggggtagcgt cacggtcagg ctcaccggct gcggctccag tccgctgttc agcagcgcat
     7681 gatgcacggc cagcaggttg agatcaccgt actgatattc cacgtttgtt gtcgggatgg
     7741 catcccggct gacgctgtcc caggtgtatt tcagcgtgcc cacctgataa ttgaacgttg
     7801 ccgcactgaa ttctgccact ttccagccat gcctgaagga gttcgcagag actcgtgtct
     7861 gaagctcatc tccttcaaac catgccagct taacattagt tgaaccatca tcgcagtaaa
     7921 tattcatcaa tatgctccgt taattacact caatttgata ccaattataa gagcaaaatg
     7981 aaaaaatcaa aacaatattt ctcaatatga gcaatataaa aatatcaaaa tgattaattt
     8041 aagcattttg aacaccttta tttactcaaa atgttcttaa ggcatgcgat ctggattatc
     8101 gtcacgctgt taaatatgtc gcggtaggga tacccgttac cgggtacccc ccgcacagat
     8161 cccggcgtgc gctattcacg caccgggctc ctgccttggg tgtctggcgg tgaaccgctc
     8221 cacaggccat ggatgcagaa cccgaacctt cggcagccat acggctgcca gtctgtttgc
     8281 tttcgtccat gtcgtatcat cctgctggct cctgcgcctc agcgcccgtc gccagaggtc
     8341 tgttacgtat atcctgaact tccgcatcat gggatagttg cccgggactg agtggtagtt
     8401 caggtatccc tgaaccactc tcctgagcca ttttccctgt tcggggattg agtaatgcca
     8461 gcgctttcgt agtccgtcct tgatcgcttt cagtgtcgcc gtcatcctgt cccggcgtgt
     8521 ctttcgtatc agcatgaacc tgccactacg gtctttccca ctgatatgcg tgaacccgag
     8581 gaagttgaac gtttctggtt taccttttcc cctgctggcg cggttttcgg ctgcgaagcg
     8641 gccgaactcc atcagtcggg ttttcttcgg atgtaccgtg agtccgaact ccttcagtct
     8701 gcgctgcatg gctatacgaa agcattgagc gtcgattcgc ttgtcgaacc cgatgactat
     8761 gtcatctgcg tatctgacca tgaccacatt gcctgtggca tggcggcgtc gccactgatg
     8821 cgcccacaga tcgaagacat agtggaggta gatgtttgcc agcagcggcg agataaccgc
     8881 accctgtggg gtgccttcct ccgatgcccg ccattctcct tcctctgagg tcccggctgt
     8941 gagccacttt cgtatgagcc tgattaccct ctggtcgccg attctgtgtt ctatgaacct
     9001 gattagccat tcatggctta ccttgtcgaa gaactgacta atatcggcat ccagtaccca
     9061 gtttacattg gtccgaacca gccctgtggc cagtgcgtcc agcgcattgt gctggcttcg
     9121 cccgggtctg aacccgtatg aaaaccccat aaagtcattt tcgtagactg cattcaggat
     9181 cttaaccagc gcatactgga cgattttgtc ctccagtgag gcgatgccga gcgggcgctg
     9241 ttttccatcc gctttcggga tgtagtgccg cctgccgggc tgagccctgt aactaccctg
     9301 atgaagcctc cggtgcagat ctgctatgtt gaccttcagg ttttcggcgt agtccttcca
     9361 cctgacacca tcaactccgg cagccgcttt cctgctcagg gagaggaatg cgttctccag
     9421 tgcctctgct gtcaggaggt ggaacaatgc ggtaaaccgt tctttcttcc gctgcttcgc
     9481 agcttcccgc acgcgtgaca gcctctgtga catactttcc cggctctgcg tccggcgcat
     9541 gtgtggctgt tccgcgtttc ccttggcccc gctccttcgc tccactgact ccgctccttg
     9601 cggattgttc gccagattcg cagctactat gagcgagtcc gacttctcct ctccgtacat
     9661 caccggcttc ggctcctcgc cttcccggtg cgggccatct ccggcactgg cagatggtca
     9721 gaggggagat ctcccggttc ccgcgtaaag atcgtattga catgccaggg tctcagaccc
     9781 cgccgggtcc atatggcact cgcagtaacg caccctatga tgttgccttc cgtaaacagt
     9841 acaacgtcgg cacccgggaa tttaatatac atttcgtggc tcaatggctg gcctgtcaac
     9901 acccctgtca acgcttcgcc ccatacctca cggtatacaa cgcatgactc ggggaccctg
     9961 tggattgctg gtccttcaat ggtcggggac tttcacccct tgatctttac cggtctcccg
    10021 gcgcacactg tatatataac cagtaaaatt agagtgtcag gaggaaagat gaagctacag
    10081 tttttcagca ccgggaaaga actcccgcca caggcccatc cgttgtttgc agacctggcc
    10141 agctgcggtt ttcccagccc tgcagctgac tatgtcgaat ctgatctgga tctccatgat
    10201 tactgcatac ggcatccgag cgcgacttac taccttcgcg ccagcggaga cagcatggcc
    10261 gacggcagcc tttacaatgg cgatctgctg gttgtggact gtgccgagaa gccccggcat
    10321 ggcgatattg ttgtggccag cgtgcagggg gagtttacgg tcaaacggct gctgctgacg
    10381 ccacgactga cgctgcagcc catgaacgcc gcctggtcgc cgatttatcc cgacccggac
    10441 gatctggata tctttggcgt cgtcacgcac atcatccacc gcccgaggga gatgtactga
    10501 tgtttgccct ggccgatgtg aacagctttt atgccagctg cgaacgtgtt ttccgcccgg
    10561 atttgaaagg aaagccggtt gtggtgctca gcaataacga tggctgcgta atcgcgagaa
    10621 gtgccgaggc aaagccctgg atcaaaatgg gtacgccgtg gtttcagatg aaaaacgaaa
    10681 gatacccgga aaaaatatac gcgttctcta gcaactatga actttatgca tccatgtcgg
    10741 cacgcgtgat gagctgcctg gaggaactgg ctccgggggt ggaacagtat tcgattgacg
    10801 agatgttcct cgatctgacc ggcgtggagc actgcatgga cctggaggac ttcggccggc
    10861 agctgcgaca gcacgtgtat gactgtaccc gtctgaccat tggcgtgggt gccggaccaa
    10921 cgaaaaccct cgcaaaatca gcccagtggg cctcaaagga gtggaaacag tttcgtggtg
    10981 tgctcgcact cacccggggg aatccacagc gaacgcggaa actgctttca ctgcagccgg
    11041 tcgaagagat ctggggcgtg ggaaaccgta ttgcacgcaa gctcaatgtt ctgggcatta
    11101 agaccgcgct cgatctggcc ctgacgaacc cggccttcat ccggaaaaat ttctccgtcg
    11161 ttctcgagcg aacagtgcgc gaactgaacg gggaaagctg catgtcgctt gaggaagcac
    11221 cgccaacaaa gcagcagata gtctgcagcc gcagctttgg tgagaaggtc actgaatacg
    11281 attcgctgcg gcaggctgtc tgtcagtacg cagagcgggc ctcagaaaag ctgcgcaagg
    11341 agcgccagta ttgccggcat atttccgttt tcatcaaaac gtctccgttc gccgttaaag
    11401 agccttatta cggcaacgtg gccacggaga aactgcttac tcccacccag gacacccggg
    11461 acatcattgc ggccgccaca acggctctgg agcgtatctg gaaggacggg caccggtatg
    11521 ccaaagccgg tgtgatgctg aatgacttca ccggctccgg cgtttcgcag ctgcagctgt
    11581 tcgacgaacg ccctccacgc cctcacagtg ctgagctgat gaaggttctc gatggcatta
    11641 atcattccgg actgggacag gtctggtttg ccggacgagg gattgcgcct gcgtggcaaa
    11701 tgaaacgcga tatgctctcc cctgcgtaca ccacccgctg gaaagatatc cccatagtga
    11761 ggatcggcta aattttgcat ttcagagaga gccagtagtg ctcgccgcag ccgaacgaca
    11821 gggctaagcc cgcgagtgag cgaggaagca ccagggaact gtacggacaa attctgctta
    11881 cgctcgaagt taaatggaca acttatccag ttatccaccg ggtagatcca agtatctgat
    11941 ccagcaaaga tccatataat gatccaaaca gatcccggta cgttgaaaac cgcgccatgc
    12001 ataggctaac agccatttca tgtgtttata agtcggatat ttgtgtttgt aaatcggtat
    12061 tctgtgttta caaagcggat ttatgtgttt ataaaacgga ttgcgcgtgt ttataaatgg
    12121 gattacgcat gctgtgtctg tggatgatcc tttaactgtg gataagtcct t
//
