LOCUS       NZ_CP084731             8731 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Acinetobacter baumannii strain HKU4 plasmid pHKU4a, complete
            sequence.
ACCESSION   NZ_CP084731
VERSION     NZ_CP084731.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN22202743
            Assembly: GCF_020520345.1
KEYWORDS    RefSeq.
SOURCE      Acinetobacter baumannii
  ORGANISM  Acinetobacter baumannii
            Bacteria; Pseudomonadota; Gammaproteobacteria; Moraxellales;
            Moraxellaceae; Acinetobacter; Acinetobacter calcoaceticus/baumannii
            complex.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Chen,J.H., Wong,S.C., Yuen,K.Y. and Cheng,V.C.
  TITLE     Genomic analysis of a new cluster of multidrug-resistant
            Acinetobacter baumannii ST-195 strains causing nosocomial
            transmissions in Hong Kong
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8731)
  AUTHORS   Chen,J.H., Wong,S.C., Yuen,K.Y. and Cheng,V.C.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2021) Microbiology, The University of Hong Kong,
            Pokfulam Road, Pokfulam 000000, Hong Kong
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. 0.4.9
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 50X
            Sequencing Technology :: Oxford Nanopore MinION; Illumina iSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:08:30
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,966
            CDSs (total)                      :: 3,871
            Genes (coding)                    :: 3,799
            CDSs (with protein)               :: 3,799
            Genes (RNA)                       :: 95
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 73
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 72
            CDSs (without protein)            :: 72
            Pseudo Genes (ambiguous residues) :: 0 of 72
            Pseudo Genes (frameshifted)       :: 18 of 72
            Pseudo Genes (incomplete)         :: 44 of 72
            Pseudo Genes (internal stop)      :: 17 of 72
            Pseudo Genes (multiple problems)  :: 7 of 72
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084731.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Acinetobacter baumannii"
                     /mol_type="genomic DNA"
                     /strain="HKU4"
                     /isolation_source="hospital"
                     /host="Homo sapiens"
                     /db_xref="taxon:470"
                     /plasmid="pHKU4a"
                     /geo_loc_name="Hong Kong"
                     /lat_lon="22.270228 N 114.130868 E"
                     /collection_date="2020-05-09"
                     /collected_by="The University of Hong Kong"
                     /genotype="ST195"
     gene            1..951
                     /gene="repM"
                     /locus_tag="LHJ65_RS19345"
                     /old_locus_tag="LHJ65_19345"
     CDS             1..951
                     /gene="repM"
                     /locus_tag="LHJ65_RS19345"
                     /old_locus_tag="LHJ65_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786597.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepM"
                     /protein_id="WP_001205343.1"
                     /translation="MRDLVVKDNALINASYNLDLVEQRLILLAIVEARESGKGINANDP
                     LEVHADSYINQFGVHRNTAYQALKDACKDLFARQFSYQEKKANGNIRNVMSRWVSQIAY
                     NDNEATVDLIFAPAVVPFITRLEEQFTKYELQQVSSLSSAYAIRLYELLIQWRSTGKTP
                     TIELQEFRKKLGVLDNEYLRMAHLKERVLELSIKQINEHTDITVKYEQHKRGRSISGFS
                     FTFKQKKKDSPSIERDPNTLELFSKMTDAQRHMFANKLSELPEMGRYSQGTESYQQFAV
                     RIAEMLQDPAQFKELYPYLKKVGYMPSNKKDTVNG"
                     /name="repM"
     gene            944..1519
                     /locus_tag="LHJ65_RS19350"
                     /old_locus_tag="LHJ65_19350"
     CDS             944..1519
                     /locus_tag="LHJ65_RS19350"
                     /old_locus_tag="LHJ65_19350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005102333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication DNA-binding protein"
                     /protein_id="WP_001096616.1"
                     /translation="MAKLSLSEVSKKFHVDRSTIYRAVRNGRLSRSSDGQFDLAEVIRC
                     FGEPEQTSQKIESSKQEGDESTKKLIAHLENEVKKYQEREERLMQQIDRMQTLIELKSV
                     APATAAPHQDATACDTKMPQHATTQQDTDNKKNNELNIAENVAVPQQETTAYHTQTLQH
                     ATLQSVAVPQHKKRGLFGRVLNAVFDND"
                     /name="plasmid replication DNA-binding protein"
     gene            1539..1682
                     /locus_tag="LHJ65_RS19355"
                     /old_locus_tag="LHJ65_19355"
     CDS             1539..1682
                     /locus_tag="LHJ65_RS19355"
                     /old_locus_tag="LHJ65_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004786602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001125246.1"
                     /translation="MPKLKDIALGIIVAPLLIPIMLIASYQDKKALKKELDECQKEKDQ
                     AS"
                     /name="hypothetical protein"
     gene            complement(1871..2389)
                     /locus_tag="LHJ65_RS19360"
                     /old_locus_tag="LHJ65_19360"
     CDS             complement(1871..2389)
                     /locus_tag="LHJ65_RS19360"
                     /old_locus_tag="LHJ65_19360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447193.1"
                     /GO_function="GO:0005515 - protein binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="WP_000447193.1"
                     /translation="METFAEPVFSNDLLAKAENGDTSAQLELAEIYLYGHGVDSDENQA
                     EVWALKSAENGNVAAMFWLADGYVTYARLMEDDDKNDSLEHFQKAFKWFQKASENGHSE
                     SMVELADLYTRADSGIEVNINKAFELREKAAKLGNKKAMRSLSVMYRDGIGIPKNTDLA
                     QSWWDKSEN"
                     /name="tetratricopeptide repeat protein"
     gene            2762..3049
                     /locus_tag="LHJ65_RS19365"
                     /old_locus_tag="LHJ65_19365"
     CDS             2762..3049
                     /locus_tag="LHJ65_RS19365"
                     /old_locus_tag="LHJ65_19365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="WP_000438826.1"
                     /translation="MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNG
                     EERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIISARQVTKAERNKYEHG"
                     /name="BrnT family toxin"
     gene            3036..3350
                     /locus_tag="LHJ65_RS19370"
                     /old_locus_tag="LHJ65_19370"
     CDS             3036..3350
                     /locus_tag="LHJ65_RS19370"
                     /old_locus_tag="LHJ65_19370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006582675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="WP_001983304.1"
                     /translation="MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEI
                     TDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQGKGYQTRMNEILRDAMLKELKNHQ
                     "
                     /name="BrnA antitoxin family protein"
     gene            3478..5889
                     /gene="znuD2"
                     /locus_tag="LHJ65_RS19375"
                     /old_locus_tag="LHJ65_19375"
     CDS             3478..5889
                     /gene="znuD2"
                     /locus_tag="LHJ65_RS19375"
                     /old_locus_tag="LHJ65_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor ZnuD2"
                     /protein_id="WP_000932953.1"
                     /translation="MLNKSKLFLALITLGASKILLAAEGPVTTLNTIVLTAQSDELGSE
                     LLGKSLNVSNQFIDTSKLKQRSTTLGDALGTELGIHSNQYGGGASTPIIRGQEGKRIKV
                     LQNNADVLDMSNMSPDHAVTVEPSLAKSIEIIRGASTLLYSSNSAAGVVNVIDYKIPTQ
                     MPQDGLEGNTTLRFNTGSNEKLTTAGVTVGLSPHVALRAEGLYRNAGNYKTPHYQSSSY
                     NSLEDLENQNSIYKNLKYLPESWAESRVGTLGLSWIDDNTYLGVSYTHRHDEYGLPAHS
                     HLYEGCGASAIGIDSRISGLKNYLLYYPQLMDEQDINYINPRPDCHQHNHIHETNFSHN
                     APYIDLNTRRYDVRGEFTQPFTGIDKIRTSLSYIDYFHNELEGDKITNFFKNTGKVGRI
                     ELSHQPLGELTGILGLQYLEQDNSALSPVHSQEGHTTYLDNQQLLNRNVTKNFSVFGLE
                     KYNWNDFTFELGARIEKQKVSMDYDIEKIKDSMKPWPNKYNSPYVEKNNKIRAQNLKSI
                     LEAVQPNKETAFSYAGTVHWRFAPNYILSLTGTHQERLPNAQEMYTHGMHLATNSFEIG
                     NRFLRKEKSNNLEISLAYKDDLLDYQISTYYYDFDNYIYLQTLNEVLGTTKVRDQHTLR
                     INHYSQSAANFYGLEGNIGYQFNSVYHGSLFGDYVKGRLTNLPDAVIAYDIWNREPTLA
                     PQKDRYTPRLPPARLGTRLKADFDESLKGEIEYYRVFKQDNISKFEQVTSGYNMLNMTL
                     AYKNKLSHTEYDLFFKANNLLDQKVYAHETFLPYIPQIGRNFSLGLNLNF"
                     /name="znuD2"
     gene            6194..6655
                     /locus_tag="LHJ65_RS19380"
                     /old_locus_tag="LHJ65_19380"
     CDS             6194..6655
                     /locus_tag="LHJ65_RS19380"
                     /old_locus_tag="LHJ65_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004781077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DIP1984 family protein"
                     /protein_id="WP_000761346.1"
                     /translation="MKLAEALLLRSDQQKKIISLKQRINANVLVQDGDQPSEDPNELLK
                     QVFSLIQEFQKLSYAIHETNALTKLNDGRSLLALLTLRDEFVEQHKTLTAAISNTSRES
                     DRYSTREIKWHKVIPVSSLQKQADDISLKLRDLNVLIQSTNWKIDLIEA"
                     /name="DIP1984 family protein"
     gene            complement(6978..7151)
                     /locus_tag="LHJ65_RS19385"
                     /old_locus_tag="LHJ65_19385"
     CDS             complement(6978..7151)
                     /locus_tag="LHJ65_RS19385"
                     /old_locus_tag="LHJ65_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001282484.1"
                     /translation="MSDNIIQLSKPCTLCENRQNVQLFAGLMLCGSCQENIRLTNPSMF
                     SANDEIEQKAQN"
                     /name="hypothetical protein"
     gene            complement(7151..7522)
                     /locus_tag="LHJ65_RS19390"
                     /old_locus_tag="LHJ65_19390"
     CDS             complement(7151..7522)
                     /locus_tag="LHJ65_RS19390"
                     /old_locus_tag="LHJ65_19390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000504218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_000504218.1"
                     /translation="MGEKVKTYAVRLDPQVAEFYDQLANSEGIRTSKLFSKILTNDFQS
                     IAVKQQADRIEDLVNRLEKRIDDFEEDNNFSEKYYEDFSGLYFMMLWLLMKNGASKDEV
                     RMIQAKGYSYADDNYKRVK"
                     /name="hypothetical protein"
     gene            complement(7794..8048)
                     /locus_tag="LHJ65_RS19880"
     CDS             complement(7794..8048)
                     /locus_tag="LHJ65_RS19880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001014303.1"
                     /translation="MNGQKIDFLRFSKQGNKYFFPCKTKHRPYVTLANFPIQDPAHAGI
                     FLSHTTHLTMTPLKALQDKDFSDFLGSQKRVQKNGLFLE"
                     /name="hypothetical protein"
     Typing          2..951
                     /name="rep_cluster_1172"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atgagagatt tagttgtaaa ggacaatgcc ttaatcaacg caagctataa cttagactta
       61 gtagaacaac gtttaatttt attggctatt gttgaagcaa gggaaagtgg gaaagggatt
      121 aatgcaaatg atccattaga agttcatgca gatagttata tcaatcaatt tggtgtacac
      181 cgtaatacag cttatcaagc cttaaaagat gcttgtaagg atttattcgc gcgtcaattt
      241 agctatcaag agaaaaaagc taatgggaat atccgaaatg ttatgagtcg ttgggtatct
      301 caaattgctt ataacgacaa tgaagcaact gtagacttaa tatttgcacc tgctgttgtt
      361 cccttcataa cccgactgga agaacaattt actaaatatg aattacagca agttagtagt
      421 cttagtagcg cttatgccat tcgcttatat gagcttctaa ttcagtggcg aagcactggt
      481 aaaaccccaa ccatagaact acaagaattt agaaagaagt taggcgttct tgataatgaa
      541 tatttacgga tggctcattt aaaagagcgt gttttagagc tttcaattaa acaaataaat
      601 gagcatacgg atataactgt aaaatatgaa cagcataaaa gaggacgttc tatttcagga
      661 ttttctttta cctttaaaca gaagaagaag gatagcccat caatagaaag agatccgaac
      721 actttggagc ttttttcaaa gatgaccgat gctcaacggc atatgtttgc aaataaactt
      781 tcagaactcc ctgaaatggg tcgctattca caaggaacag aaagctacca acagtttgct
      841 gtacgtattg ctgagatgct acaagatccc gctcaattca aagagctata cccataccta
      901 aaaaaagtgg gatacatgcc atcaaataaa aaggacaccg taaatggcta agttatcact
      961 aagtgaagta tctaaaaaat ttcatgtgga tagatcaacc atttacagag ctgtacgtaa
     1021 tggacgttta tcacgctcca gtgatggaca attcgatcta gcagaggtca tacgatgctt
     1081 tggagaacct gagcaaacat ctcaaaaaat tgaatcatct aagcaagaag gtgatgaatc
     1141 tacaaaaaaa cttattgccc atttagaaaa tgaagtcaaa aaataccaag aacgtgaaga
     1201 acggttaatg caacaaattg accgtatgca aacactcatt gagctgaaaa gtgttgcacc
     1261 tgccacagca gcaccacacc aagatgctac ggcatgcgac accaagatgc cacagcatgc
     1321 gacaacacaa caagacactg ataacaaaaa gaataatgaa ttaaatattg cagaaaatgt
     1381 agcagtgcca cagcaagaaa ctacggcata ccacacccaa acgctacagc atgccacgtt
     1441 gcaaagtgtg gcagtgccac aacacaaaaa acgtggttta tttggccgtg tgctgaatgc
     1501 cgtctttgat aatgactgag caataagggt gaaagatcat gccgaaactg aaagacattg
     1561 ccctgggaat tattgtagct ccgctgctga tcccgatcat gctgattgca tcgtaccagg
     1621 ataaaaaggc gctgaagaaa gagctggatg aatgccaaaa agaaaaagac caggcatcct
     1681 gattattcaa ttgagctaat caaaagtgtg gaacattgat gaaataatag cgtggaacgt
     1741 gaaaagtcat aaaaaaagcc cgaacaggga tgctcgggct ataaactaga aactacagcg
     1801 ttgaattata aggataatta tacagcattt cgtataaggt gtattatgtt aattttagga
     1861 aatattaaaa ctaattttca gacttatccc accaactttg agctaaatca gtatttttag
     1921 gaatacctat accatcacga tacataacgg aaagacttcg cattgctttc ttattaccta
     1981 actttgcagc cttttcacga agttcaaaag ctttattaat attaacttct attccgctat
     2041 ctgcacgagt atatagatca gctaactcaa ccattgattc agaatggcca ttttctgagg
     2101 ctttctgaaa ccacttaaaa gctttttgga aatgttctaa agaatcgttt ttgtcatcat
     2161 cttccattaa tctagcataa gtaacatatc catcagctaa ccaaaacatt gctgctacat
     2221 ttccattttc tgctgattta agagcccaaa cttcagcttg attttcatct gaatcaacac
     2281 catgaccata tagataaatc tcggctaatt ctaactgagc agaagtatct ccattttctg
     2341 ctttcgctaa taaatcatta gaaaaaacag gttctgcaaa tgtttccatt gaaaggcaaa
     2401 ctcctaaagc cataaaaata tttgttaatt tattcaattt ttaaccttta aactaaactg
     2461 ttaggcttgt atatttttat atcataccta aaagaaaact ctaaaattag agtatgtctt
     2521 atctctattt aacataatgg cgattatacg aagtacactt gtatattaat ataaatatca
     2581 actacttaat aaaaatagaa aaatcccaaa atgcccaaaa agccgacttt gaaacaagtc
     2641 ggctttttta tgagcagttt tgatacttct tgccaataat tttgactaaa aattacacga
     2701 atctggttga gtgatacaat tttgtgatac attattgtat atacaaattt gaatgttttt
     2761 tatggaacag tattttgaat gggatgaggc taagaatcga aagaatcaga aaaagcatga
     2821 tatctctttc gaaactgcaa gccttgtttt tgaagatcca ttacggatct caatccaaga
     2881 cagacatacc aatggtgaag aacgttggca aaccattgga cgagttaaag gcgtactaat
     2941 gttattggtg gctcacacca tttttgatga agatgactgt gaaatcatcc gaatcattag
     3001 tgcaaggcaa gtcactaaag cggagcgaaa caaatatgag catggttaga tactcacaca
     3061 aagagctgaa tgagaaattt ggtgagaagc aagatgctga aattcaacgc ttgcttgcta
     3121 aaggcacggt tcctgatgat cagctagatc tatcagatat tcctgaaatt acagactgga
     3181 gcaatgctgt acgccaaaat caattttacc gcccagtaaa gcaacaaact tcaattcggt
     3241 tagatgctga tgtacttgct tggttcaaag cacaaggcaa aggttatcaa acacgtatga
     3301 atgaaatttt acgagatgct atgctaaaag aattaaaaaa tcatcaataa gaatgggagc
     3361 ttaggctccc atttttgact tcatttttag gggggattac cattcaaaaa tttaaatagt
     3421 agattttata aatttttaag tgttatatta taatataaca ttaaatttgg ttgttttatg
     3481 ctaaataaaa gtaaactatt tttagcttta attactttag gggcctcgaa aattttattg
     3541 gcagctgaag ggcctgttac taccttaaat acaatagtat taactgcaca atctgatgag
     3601 ttagggtctg agctgttagg caaaagtcta aatgttagta atcagtttat tgatactagt
     3661 aaacttaaac agcgttcgac aactctaggg gatgccttgg gtacggaatt aggaattcat
     3721 tctaatcaat atggaggtgg agcatctact cctattattc gtgggcagga aggaaaacgt
     3781 attaaagttt tacagaataa tgctgacgtg ttggatatgt ctaatatgtc cccagatcac
     3841 gctgtcacag ttgaaccaag tttagctaag tctattgaaa ttatacgtgg tgcaagtacc
     3901 cttttgtata gttcaaatag tgcggcaggg gtagtcaatg tcattgacta taaaattcca
     3961 acccaaatgc ctcaagacgg attagagggt aatactacat tacgttttaa tactggcagt
     4021 aatgagaagt tgaccacagc aggtgtaaca gttggcttaa gcccacatgt agctttacgg
     4081 gcagaagggt tatatcgtaa tgctggtaac tataaaactc ctcattacca gtctagctct
     4141 tataattctt tagaagatct tgaaaaccaa aacagtattt ataaaaattt aaaatattta
     4201 ccagaaagct gggcagagtc gagagtaggt acgcttggat tatcctggat agacgataat
     4261 acttatttag gagtttctta tacacatcgt catgatgaat atggtttacc agcccatagc
     4321 catttatatg aaggttgtgg tgcctctgca atcggtatcg attcacgaat tagtggatta
     4381 aaaaattatc ttttatatta tcctcaattg atggatgagc aagatattaa ttacataaat
     4441 ccacgtcctg attgtcatca gcataatcat attcatgaaa ctaatttctc tcataatgct
     4501 ccttatattg atttaaatac tcgacgttat gatgtgcgtg gagaattcac tcaaccattt
     4561 accggtattg acaaaattcg tacctcattg agttatatag attattttca caatgaactc
     4621 gagggagata agattactaa cttttttaaa aatacaggaa aagtaggccg catagagtta
     4681 tcacatcagc cattagggga attaaccggt atcttggggc tacaatattt ggaacaggat
     4741 aatagtgctt tatcaccagt ccattctcaa gaaggccata ccacctatct agataatcag
     4801 cagctattaa atcgaaatgt cactaaaaac tttagtgtat ttggcctaga aaaatataac
     4861 tggaacgact ttactttcga actaggtgct cgtatagaaa agcagaaagt tagtatggat
     4921 tatgatatag aaaaaattaa agattcaatg aaaccttggc ctaataaata taacagtcct
     4981 tatgtagaaa aaaataataa aatcagggct caaaatttaa aatctatttt agaagctgtt
     5041 caacctaaca aagaaacagc tttttcttat gcaggtacag tccactggag atttgctcca
     5101 aattatatct tatcattaac aggtacacat caagaaagat tacctaatgc acaagaaatg
     5161 tatactcatg gtatgcatct cgctactaat tcttttgaaa taggtaaccg ctttctaaga
     5221 aaagagaaat ccaataattt agaaatcagt ctggcctata aggatgattt acttgattat
     5281 caaatcagta cttactatta tgattttgat aactatatat atttacagac actcaatgaa
     5341 gttttaggaa ctacaaaagt tagagatcag catacactac gtattaatca ttatagccag
     5401 tctgctgcta atttttatgg attagagggc aacatagggt atcaatttaa ttctgtttat
     5461 cacggctctc tttttggaga ttatgtgaaa ggccgtttaa caaatttacc tgatgctgta
     5521 attgcatacg atatctggaa tagagaaccg actttagctc cccaaaagga tcgttacact
     5581 cctagattac ctcctgctcg tttgggtaca cgactaaaag ctgattttga tgaatcgctt
     5641 aaaggtgaaa tagaatatta tcgagtcttt aaacaagata atatttctaa atttgagcag
     5701 gttacatcag gttataatat gcttaatatg actttagcat ataaaaataa gctatctcac
     5761 acagaatatg atttattctt taaagcgaat aatttacttg atcagaaagt ctatgcccat
     5821 gaaacatttt tgccatatat tccccaaatt ggaagaaatt ttagtttagg cttaaatctt
     5881 aatttctaga taagatactg taatcttcta agaaaatttc ttagaagatt tttcttataa
     5941 aatgattaga ttaataatta aggtaagcgt ccgctgaatc ccatacctat tttttaattc
     6001 gatttaggtt tccagcagtg tggcagtaac tcttcaatct gggtcacttt atgtgtcggc
     6061 agccttttca gcactgaata gccaccgatt ttgtagacac cttttagcta gataaaaagc
     6121 aatttttttg atctttaaag aatgaggata taagctgttt aaaacttaat tctaattggt
     6181 taataggtga aaaatgaaat tagcagaagc tctcttactt cgaagtgatc aacaaaaaaa
     6241 aattatttca ctaaaacaac gtatcaatgc aaatgtattg gtacaagatg gtgatcaacc
     6301 atctgaagat ccaaatgaac tactaaaaca agtattttct ttaattcaag aatttcaaaa
     6361 attaagttat gcaattcatg aaactaatgc tttaacaaaa ttaaatgatg gccgttctct
     6421 acttgcttta ttgactttgc gtgatgaatt tgtagaacag cacaaaaccc taactgcagc
     6481 aataagtaat acatctcgtg aatctgatcg ttatagtacg cgggaaataa agtggcataa
     6541 agttattcca gtttcatcat tacaaaaaca agctgatgat attagtctta aattacgtga
     6601 tttaaacgtg ttaattcagt cgactaattg gaaaattgat ttgattgaag cctaatagtc
     6661 ctttagatat aatcaagttg aacatactgg gcgagtacaa gatcccttac ttccacaaca
     6721 taggattgta aataaagtgt agtggcttgc aaagcaagtg gataagctta aaattttaag
     6781 tacaaatcaa tccatcactt ggcataactc aaatctttgt gcaaacaaag ttacacgtta
     6841 cttataacta ccatgtactg acagtatgtt cacctcaatt actaaaaata aagaaatgaa
     6901 caagcatcag ctcaactaca tttctatttt taattcagtt cggcatttaa cataaaatct
     6961 cttatacgaa gcccaggtta attctgggct ttttgttcaa tctcatcatt ggccgagaac
     7021 atacttggat tcgtaagcct gatattttcc tgacaacttc cacacagcat caatcctgca
     7081 aaaagttgta cattttgtct attttcacaa agcgtacatg gctttgaaag ctgaataata
     7141 ttatctgaca ctactttacc ctcttatagt tatcatctgc atatgaataa cctttagctt
     7201 gtatcattct aacctcatct ttagaagcgc catttttcat taagagccac aacatcatga
     7261 aatatagtcc agaaaagtct tcatagtatt tttcagaaaa attgttatct tcctcaaaat
     7321 catctattcg cttttctaat cgattcacca gatcctcaat acgatcggct tgctgtttaa
     7381 cagcaataga ttgaaaatca ttggtcaaaa ttttactgaa taatttactg gttctgattc
     7441 cttctgaatt agccaattga tcgtagaatt ctgctacttg tggatctaac ctgactgcat
     7501 aagttttgac tttttcgccc ataaattaca cctcttatta aaattagatt ttgtacaaaa
     7561 ttaaatatat ataattcaac taattaaata aaaaataata attagattac gtacaaactt
     7621 atttgtacag aattaattta ttctaaaaca aaaacttatt taaatataat gcttttttta
     7681 tgtacaatca tgagtacata ttttttctta aatttatcaa tatcttatct aaaaaaagtg
     7741 ttgttttgat gtacaaccat gagcacagca taaaaaatag ataattcaat cacttattct
     7801 aaaaaaagac catttttctg tacacgtttc tgagacccta aaaaatcgct gaaatcctta
     7861 tcctgtaagg ctttgagggg tgtcatcgtt agatgcgtgg tgtgtgacaa aaaaatcccg
     7921 gcatgtgccg gatcttggat tggaaaattg gctaaagtga cgtagggccg gtgcttggtt
     7981 ttgcatggaa aaaagtattt atttccttgt ttagaaaaac gtaaaaaatc aattttttgt
     8041 ccattcatcc aggttaaaaa tttcaaccca aaactttaat tatgaaaagc ttcatagaac
     8101 gcgttcaaat gcgatttaag agcctttacc tagaaaacat agatctcata gtgaaaaaca
     8161 caaaacagct caaaaaacgc aaatgagagc gaaataaaga ggtattttga ctttagatag
     8221 atgcatagag cgagtgtcta cgagcgaact atcaaaattt gcgcctagac cctctgaaaa
     8281 acattttttt gtcctcttta gcctaagaaa gcgtaagttt cgtgcagaaa tttgctcctg
     8341 gatcgagcgt agcgagtaaa aaagttttat gagcgaagcg aattccaagt ttcttttgct
     8401 tttttttaaa gtcacgccag tattaaccaa aaaattgccc caacgaactg agcgaaagcg
     8461 aagttcaata gagtttgagc gaagcgaaaa ccaagggcaa tttttcattc cctgggcttt
     8521 taattatttt aaagttttta aatactttta aatggcttga aagccttatg aataaagggt
     8581 tttagctctt atatgtccac gtttaccttg caatatgtcc acgtttacct tgcaatatgt
     8641 ccacgtttac cttgcaatat gtccacgttt accttgcatt agtacacaaa taatattaac
     8701 gtgtacttat acacaataaa aaatagtggc t
//
