LOCUS       NZ_CP084709            11972 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain KP1064WHY plasmid p4, complete
            sequence.
ACCESSION   NZ_CP084709
VERSION     NZ_CP084709.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21219937
            Assembly: GCF_020520185.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11972)
  AUTHORS   Dong,S.
  TITLE     Whole genome of Klebsiella pneumoniae strain KP1064WHY
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11972)
  AUTHORS   Dong,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-OCT-2021) Department of Clinical Laboratory, Zhejiang
            Hospital, 12 Lingyin Road, Xihu District, Hangzhou 310013, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: minimap v. 2.0; miniasm v. 2.0
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 300.0x
            Sequencing Technology :: Oxford Nanopore GridION; Illumina HiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:20:50
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 6,022
            CDSs (total)                      :: 5,900
            Genes (coding)                    :: 5,530
            CDSs (with protein)               :: 5,530
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 370
            CDSs (without protein)            :: 370
            Pseudo Genes (ambiguous residues) :: 0 of 370
            Pseudo Genes (frameshifted)       :: 234 of 370
            Pseudo Genes (incomplete)         :: 141 of 370
            Pseudo Genes (internal stop)      :: 33 of 370
            Pseudo Genes (multiple problems)  :: 38 of 370
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084709.1.
            Bacteria and source DNA available from Department of Clinical
            Laboratory, Zhejiang Hospital.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11972
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="KP1064WHY"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p4"
                     /geo_loc_name="China: zhejiang"
                     /collection_date="2019-07-08"
     gene            510..659
                     /locus_tag="K8U55_RS30265"
     CDS             510..659
                     /locus_tag="K8U55_RS30265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_324170203.1"
                     /translation="MGCKHEPPVQPDCCALSGNYHLESNPDGHAKTPLAAAIGNWEFAE
                     DLFS"
                     /name="replication initiation protein"
     gene            complement(874..1023)
                     /locus_tag="K8U55_RS29965"
                     /old_locus_tag="K8U55_29960"
     CDS             complement(874..1023)
                     /locus_tag="K8U55_RS29965"
                     /old_locus_tag="K8U55_29960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(1108..1365)
                     /locus_tag="K8U55_RS29970"
                     /old_locus_tag="K8U55_29965"
     CDS             complement(1108..1365)
                     /locus_tag="K8U55_RS29970"
                     /old_locus_tag="K8U55_29965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(1375..3060)
                     /locus_tag="K8U55_RS29975"
                     /old_locus_tag="K8U55_29970"
     CDS             complement(1375..3060)
                     /locus_tag="K8U55_RS29975"
                     /old_locus_tag="K8U55_29970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(3165..4652)
                     /gene="ltrA"
                     /locus_tag="K8U55_RS29980"
                     /old_locus_tag="K8U55_29975"
     CDS             complement(3165..4652)
                     /gene="ltrA"
                     /locus_tag="K8U55_RS29980"
                     /old_locus_tag="K8U55_29975"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     gene            5297..5542
                     /locus_tag="K8U55_RS29985"
                     /old_locus_tag="K8U55_29980"
     CDS             5297..5542
                     /locus_tag="K8U55_RS29985"
                     /old_locus_tag="K8U55_29980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            5816..6187
                     /gene="tolA"
                     /locus_tag="K8U55_RS29990"
                     /old_locus_tag="K8U55_29985"
     CDS             5816..6187
                     /gene="tolA"
                     /locus_tag="K8U55_RS29990"
                     /old_locus_tag="K8U55_29985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6184..6549
                     /locus_tag="K8U55_RS29995"
                     /old_locus_tag="K8U55_29990"
     CDS             6184..6549
                     /locus_tag="K8U55_RS29995"
                     /old_locus_tag="K8U55_29990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            6724..7002
                     /locus_tag="K8U55_RS30000"
                     /old_locus_tag="K8U55_29995"
     CDS             6724..7002
                     /locus_tag="K8U55_RS30000"
                     /old_locus_tag="K8U55_29995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            6990..7304
                     /locus_tag="K8U55_RS30005"
                     /old_locus_tag="K8U55_30000"
     CDS             6990..7304
                     /locus_tag="K8U55_RS30005"
                     /old_locus_tag="K8U55_30000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7468..7896
                     /locus_tag="K8U55_RS30010"
                     /old_locus_tag="K8U55_30005"
     CDS             7468..7896
                     /locus_tag="K8U55_RS30010"
                     /old_locus_tag="K8U55_30005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            7922..8101
                     /locus_tag="K8U55_RS30015"
                     /old_locus_tag="K8U55_30010"
     CDS             7922..8101
                     /locus_tag="K8U55_RS30015"
                     /old_locus_tag="K8U55_30010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8128..8658)
                     /locus_tag="K8U55_RS30020"
                     /old_locus_tag="K8U55_30015"
     CDS             complement(8128..8658)
                     /locus_tag="K8U55_RS30020"
                     /old_locus_tag="K8U55_30015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_226522486.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLARQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8665..9395)
                     /gene="mobC"
                     /locus_tag="K8U55_RS30025"
                     /old_locus_tag="K8U55_30020"
                     /pseudo=""
     CDS             complement(8665..9395)
                     /gene="mobC"
                     /locus_tag="K8U55_RS30025"
                     /old_locus_tag="K8U55_30020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /name="mobC"
     gene            complement(9395..11359)
                     /locus_tag="K8U55_RS30030"
                     /old_locus_tag="K8U55_30025"
     CDS             complement(9395..11359)
                     /locus_tag="K8U55_RS30030"
                     /old_locus_tag="K8U55_30025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     BGC             1..8060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          192..771
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="96.569"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8669..9237)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="92.105"
                     /coverage="100.0"
                     /evalue="1.34e-114"
ORIGIN
        1 agggaataga aagacttaat caggggacaa tatcccacat cagcgcgtcc ggtgaccgtc
       61 ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg tcgctacgct ccgggcgtga
      121 gactgcggcg ggcgctgcgg acacatacaa agttacccac agattccgtg gataagcagg
      181 ggactaacat gtgaggcaaa acggcagggc cgcgccggtg gcgtttttcc ataggctccg
      241 ccctcctgcc agagttcaca taaacagacg ctttttcggt gcatctgtgg gagccgtgag
      301 gctcaaccat gaatctgaca gtacgggcga aacccgacag gacttaaaga tccccaccgt
      361 ttccggtagg tcgctccctc ttgcgctctc ctgttccgac cctgccgttt accggatacc
      421 tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc atagctcaca cactggtatc
      481 tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa gcacgaaccc cccgttcagc
      541 ccgactgctg cgccctttcc ggtaactatc atcttgagtc caacccggac ggacacgcaa
      601 aaacgccact ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa
      661 cacgcggttg ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt
      721 tgctgtgctc tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt
      781 cgatcaaacc acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa
      841 aaaaaggatc tcaagaagat cctttgatct tttctactga accgcgatcc ccgtcagttc
      901 agaggaggac gatggtgcca ccgttccgcc ctgaacatcc cggatataat ttgcctgaca
      961 tgcggccaga agaaagccag caacaatcaa tataaaaaga aaaatagtct ttaccttttt
     1021 catatacttt ccctgtattt aaagactccg gcattcatcc ctgaacaccg gaagtcagta
     1081 tttcaaatca tccttgataa ttttttgtta ccagttaccg tcccgataat caaaagctat
     1141 ataacaatta tccacatcag gttttatttg atttttaaaa aatggctgta gtaaaggaac
     1201 ccattctttc ttcaaatcaa actcaccatt attaatatta tcctttaaag gcattcccaa
     1261 gctttcaatg acagaaccat cgtcaccaaa gtctttagaa tactcttccc ctttaaactc
     1321 ttcagttttc ttatcaaacc atgtcaaacg taatttaagc cccatattta cctctcaaag
     1381 atatttttta atgttccgtt ttggatctgg ccctttaacc tgcttacccg ttttgggatc
     1441 aaatgcgcct aggtgttcgc catcactggc acgataccct tcaagctcac cgtgctggga
     1501 atcccattca taaattttac gttttttatc gccataccat cgggcccgct taccaccacc
     1561 accttgttta ggtgttttag gatcacctcg ctttaactct ccaagtccct ttatgtcttc
     1621 tgttttaggt actggatgat aatcatggcc gtagtcttta gtccccttgc gaggcttgtt
     1681 cttttcctca tttaacttat tttctgcatc cttcgctttt ttctcctttt gtttccggct
     1741 ctccatcgcc ccggtaagtg cagccttatc tctggataag gattcattca ttgcgtttaa
     1801 agatgcctga gccgctttta atttctcatt ctgattctga atatccttat caagatagcg
     1861 aacctgatcc tgccacccgg agccatattc atgaggcggg ttataagtca caaaatcatc
     1921 tttatttgcc tctgcctctt ttaccttttt atcggcttcc tgaacagcag cccttgcacc
     1981 ggggagcgcc ttctctgtcg atgcaatgcg gctgtttaag gtcgcaatat ttttatcttc
     2041 ggcatccagc tcagctttcg ctttatcata gtctcttttc agcccctctt ccggatgagc
     2101 ggcgtcccat gcctgctggc gacgcttttc ttcctcctga cgctgtttta cctgtgccgg
     2161 ggtaagaaca tctgtcaccg acacataaac cggcttctgt ccggtctctt tcgggaaacg
     2221 aataacggcc tcacgggagt taccaccggc tgtaaaacca gccggacgtg aatcaccttt
     2281 ttcagcaaca atgccttttg gcggggcttt cgctgtcgga acacctttcg gtacgctcac
     2341 ctgcagagac gggagacccg gaatcgacac actgaatacc cccggacgtt ttgtcggttt
     2401 cgcatccacc acagggacac tcattggccg acctgcgaca accgcaatat gctgccgctc
     2461 atccttcacc acatccacaa cccgttgtct gacacgaacc gtcgcctggt ccagtggtaa
     2521 agtacttgcc ggagtctccg tcaccgcatc ggccggtaat gacgtcataa tttttgacat
     2581 cattttcgga tcatcttttg caatctcaga aggcagcaca ccgtagatcg caatccccca
     2641 cagaccaaac ttaaacggcc ccttcagggc agccagcaca tcagcaacgg cggccgacaa
     2701 cgcatcgccg gaaactgata aagccagtcc ttcagccccc ggagtagcca gggcaggcag
     2761 accgaaggcc atcgcagtgg ccgaagactg tccgccacca gagctaccgc tatgattgcc
     2821 gctgttacca ccattaccac cgggtttact cccgccgtta ccaaactccc ccggattata
     2881 gttatgaata tctccattcg gcgtatgcgt cgtaccccag cccggcccgc tgtttggatc
     2941 cgtaccacca cctgatgaag ggccaccttt accagacgta ccgttcacat tcccactggc
     3001 ctgaccacca ttatgtccca gacctgaatt acccggacct cgaccgtctc caccactcat
     3061 accgttttcc tctttgacat ttaaaacaat aaattaaagc caacatgtcg cggtagggat
     3121 acccgttacc ggataccccc cgcacagatc ccggcgtgcg cgatttacgc accgggctcc
     3181 tgcctcgggt gtctggcggt gaaccgctcc acaggccatg gatgaagaac ccgaaccctt
     3241 ggtagccatg cggctgccag tttgtttgct ttcgtccagg tcgtatcatc cttctggctc
     3301 ctgcgcctga gcgcccggcg ccagaggttt gttacgtgtg tcctgaactt ctgcatggtg
     3361 gggaagttgc ccggtaccga gtgatagttc aggtatccct gaaccactct cctgagccat
     3421 tttccctgtt cggggattga gtaatgccag cgccttcgca gaccgtcttt gatggctttc
     3481 agagttgccg tcatccgatc ccggcgggtc tttcgtatca gcatgaacct gccgttgcga
     3541 tctttcccgc tgatgtgcgt gaacccgagg aagttgaacg tttctggttt gccttttccc
     3601 ctgatggcac ggttttcggc agcgaagcgg ccgaactcca tcagacgggt tttctccggg
     3661 tgaaccgtga gtccgaactc cctcagtctg cgctgcatgg ctatacggaa gcgccgggca
     3721 tcgtatcgtt tgtcgaaccc gatgacgatg tcatcggcgt atctgaccat taccacattg
     3781 cctgtggcat agcgacgtcg ccactgatgc gcccacagat cgaagacgta gtggaggtat
     3841 atgtttgcca gcagcggtga gatgaccgca ccctgtgggg tgccttcctc cgttgctcgc
     3901 cattgaccct cctccgacgt cccggctgtg agccacttac gtatgagcct gattaccctc
     3961 cggtcgccga tccgatgctc tgtgaacctg atcagccatt cgtggctcac cctgtcgaag
     4021 aactgactga tgtcggcatc cagtacccag tttacgttag tgcgtaccag ccctgtggcc
     4081 agtgcgtcca gtgcatcgtg ctggcttcgc ccgggtctga acccgtatga gaaccccata
     4141 aagtcgtttt catagactgc gttcaggatt ttcaccagcg catactggac gatcttgtcc
     4201 tccagcgagg cgatgccgag cgggcgttgt tttccatccg cttttgggat gtagtgacgc
     4261 ctgccgggct gcgccctgta gctgccctga tgtagcctcc ggtgcagatc tgttatgttg
     4321 ttcttcatgt ttccggcgta gtccatccac ctgatgccat ccactccggc ggccgctttc
     4381 ctgctcaggg agaggaatgc ggcttccagt gcttcgactg tcagcaggtg gaacaatgct
     4441 gtaaaccgtt ctttcttccg ctgcttcgca gcttcccgca cgcgtgacag cctctgtgac
     4501 atgctttccc ggctctgtgt ccggcgcatg tgtggctgtt ccgcgttccc cttggccccg
     4561 ctccttcgct ccactgactc cgctcctttc gggttgttcg cctgcttcgc cgctactatg
     4621 agcgagtccg acttctcctc tccgtacatc accggctatg actcctcgtc ttcccggtgc
     4681 gggccatctc cgacactggc agatggtcag aggggagatc tcccggttcc cgcgtagaga
     4741 tcgtattgac atgccagggt ctcagacccc gccgggtcca tgtggcactc gcagtgtcgc
     4801 accctatgat gttgccttcc gttaacagta caacgtcggc acccggtaat ttaatataca
     4861 tttcgtggct caatggctgg cctgtcaaca cccctgtcaa cgcttcgccc catacctcgc
     4921 ggtatgcaac gcatgactcg gggaccttgt ggattgctgg tccttcaatg gtcggggact
     4981 ttcacccctt gatctctaac cggtctcccg gcgcacactg tacatataca cagtataatt
     5041 attaccttac tcctcccgtt ttcaggtgtc aatatatgag attagttctc atcttcaggc
     5101 acaaaaaagc gcccggagcc acctgtaagc agatcgcgtc ttttcccgaa aatttctcat
     5161 ggcaacaaat tttattattt aagacattca tcacacttac atttcagatg gtatagacac
     5221 atctgaaagc taactggcat gaggagagga cctgcggtta aactgttcgg gaaacctgag
     5281 acgcccggag gccactatga cgcacctgaa actgactccc gctgaagaaa agcgcctggc
     5341 ggacgccttt gtcgctcagg cactggccat gggcctgaaa gggaacaaaa cgctgccaaa
     5401 ggcggtaaaa caacaggtgc gggaaagggt gcttcgtgcc attcaggacg aacgtgaagc
     5461 caggcgggcg caggaaatgc gtcgggagaa atacgaggct acggaaacca ccttcacctg
     5521 gcagccacag cgccgccggt aaggggtatt taccataacg tcgcattgcc cgcaccgctc
     5581 agacggtctg agggcgtttc cggcggctac gggataaaac aggcaacaaa ccacccggaa
     5641 aaccgccgga aacgcgcccg gtaaattgta acccgctgca tgcctatgca cgcaactgca
     5701 tggcgggccg tttggtgtaa aaacgatacg ataagccgtg tttatgtaaa tgaacgtggc
     5761 cgtaaagagt gacaaatata catgtaacat tttttactga caggtcatca acccgatgcg
     5821 tttaataccc tttatcctgc tggcattgtg cagcccctta ctcctgacgg cctgtaagca
     5881 ttccaccacc cagccgccga aaatttatac tctggataag tcatctaaaa tcagtagcta
     5941 cgctgcggcc atcaagaccg ctgttgaaat gcagatgttt gaaccagagg cctatgcggg
     6001 aaaaatgtgc accgtgcgga tatccataca acgtgatggc actctgaaca gcgccattgc
     6061 tgaaggagga gatccgaaac tctgtaaagc agcaatatca gccgttaccc acgccaaaat
     6121 tccgccggca ccggatgaaa atacctggca aatattcaaa aacgcccctc tggatttcag
     6181 accatgaaag cttttactct gacagtgctg gcggccatgt tcctcactcc ggtacatgcg
     6241 ataccactaa aacccgtaac cacactgcag ccaacctacc cggtacaggc cgcagcaaaa
     6301 cgcatcgaag gtcaggtgac cgccacgttt gatattctgg acgatggacg agtggacaac
     6361 gtgtcgctta ccggctgtca cctttttttt cgggaaacgc tgacggcaat gaggcactgg
     6421 ctatatcccg cagggcaccc ggcaatgcga caggttataa attttgaatt cagtctgaaa
     6481 aatgacggct tacaggatcc gcgaccagtc accacccaaa agctcaatga tgatggttgc
     6541 cgccagtaac aggtccttta acccgcaggc tgcaatagtc ctggatgatg agtgagcctg
     6601 tagcagccct gattagtgga agcgaagctg aaaaatgggc agcaaaagcc gtatttctgg
     6661 caaatagcgc cgccacggat taaagtagtc atgtgtaata catatgacta caaaggggcc
     6721 tttatgtcat cagcaacatc catcaggctc gatgaagaac ttaaggaccg gctgaaaacg
     6781 ctggccgatg accggcaccg ctcagcgcac gcgctaatgc tggaggcaat caccgaatac
     6841 atcgaccggg aagagaagcg cagccagtac ctgcgggacg gacaggccgc atggcagcac
     6901 tatcaggaaa cgggtctgca ccttactgcg gaagaagcgg aagcctggat aagtacctgg
     6961 ggaactgaga acgagcagga tgctccgcca tgccacaggt aacgatttcc gcgctggcac
     7021 agcgcgattt acaacgcctt caggactttc tcaaaaccaa aaatcggctg gcagccagaa
     7081 aaggaggtga ggtgatcgtc cgggctatcc agcaactgaa aacattgcca gacattggcc
     7141 gcccggtgcc gtttctgccg ctggaatata aggaactggt gatcgggttt ggcgacagtg
     7201 gctatgtgat gctctaccgc cacgaccggg aaatggacca gattgtgatc gtcaccgtca
     7261 ggcatcagaa agaatccggg tatccggggg cagacagcct ctaataaccg atgacggggg
     7321 cgcaattcat tgcgtggccg tcgccggttt gacaagcgca gcgcgtcaga aaaccacatc
     7381 ctgaacggaa aaacgggtaa aattcagcga tacatttacc gtgagaaatt acacaccttt
     7441 cacacccgat aaaatcaggg gtatacagtg gcgaaaacat tgctcaacct ttcacaggca
     7501 gcacaagccg cgggcattac cagaagaacg ctatacaatc atgtaaaaca gggaaaggtt
     7561 accgtgtcac gtgacgggaa aaacaaccct gtcgtggatg tgtcagaact gatccgtgta
     7621 tacggaaatg tgaatatccc tgagaaacaa attcccggca tttcacaccg tgaaaataca
     7681 caaaaaaatt tcccacagga acagcttctg gcaatgcaaa aggaactggc cgatctccgg
     7741 caggctgtca ccctgatgct ggaagataaa acatcccggg aagaagaacg ccgccagcac
     7801 gatgatgagc gccggaaact ccaggccgaa gtggacagat tgacaacaga actgacacag
     7861 aagaaaaaac gcttctggtc cggctggttt agctgagaac ttaccgaagg gaatggagaa
     7921 catgacgaag caggaaaaaa ctgccctcaa tatggctaaa ttcattaagg atcagtcgct
     7981 gctgttgctg gaaaagctga acgagctgga catggatacc ccggccgaca tgtgcgaacg
     8041 gcttcacgag gatgcggaaa cactctggca ggtgatgcgg gataagctcg gggaagaata
     8101 acagcaccgg ccatgccggt gctggcgtta ttttttgccg aacggccacc accggcgggc
     8161 gggtttgtcc tccggcagtt caggcaccgg cacgttaaaa tagcggctgt tatattcccg
     8221 catcagctcc gctttcagct tctcgttcac cgcgctttcc ccgtgccgct cgctgaccac
     8281 ctcaatcatc gcgttaatct gctccggtga gcacttcccg gcatcaacca cctgcacacc
     8341 gttctcgatg gcgaaccgct ccacctttcg atagaggtaa tcacacagct tcattcctgt
     8401 gggtttgtcc cgttcccatt gcggattttc accggtgaaa aagtacgtgg ctttttcgtc
     8461 gctgttgagc cgctcgtcat acatcccgat ggatttcatc atcgccagat gctgggcccg
     8521 gtcgacaaaa cagaaaacat acgccccggt ctgccgtgcg agcaccacca tgtcttccag
     8581 atgctcgctg gttggcataa tgcagcgctc gccgtctttg tcttccacgt cctcaataaa
     8641 taccatgtac agcgccataa tccctcactc cgttgtcata aatttaaagc acgcgaacag
     8701 cctctcccgg tccagctcca ccgttttgcg ggcctcgccg gtctgtggat tcacgatttc
     8761 atccaccgcc agaatcgccc gctgtacccg gacaagcgtc ggccggtcag ggcagatgta
     8821 ccagatttca tcgatgccgt tcgcccggcg gttgaacaga tacccggaca ccacttcctg
     8881 ataccgcctg cggctcttga cggtgcgctc tacctcaaat gccaccgttc tgccgtccag
     8941 ctccgccagc gcgtccggca ctttcatccc tttcagggcc atacggtgca ggtaccgcca
     9001 gccggtggcc ccccggccct ccatggagag gcgcagggac tgcacggcca gccggtgcgg
     9061 cagctgcgcc gccgacaccc ttgacggctg aaacggaata acgtccgtca gcggctcgtc
     9121 ctcgtcgaac gagaacgcca ccccgtgcgg cgtcagcccc cacacgctga cctgacggcc
     9181 gtcaaccggg tatttcgccc ggaccagcag ccctttcgcc accagactgt ccagaagcgg
     9241 taaaccggct gtaccgccgc aatccccagc cgtcgcgcaa ccagctccgc cgtggaacac
     9301 agctcttccc gcagccagcg cagggtcagc cgtgttttct ccgcgttccg cgcctgccgc
     9361 ttcgcgtggc tgacgttgta ctgctccagt gtcatcagta aagccccgca aaatcatcat
     9421 ccgcttttgg tccgccgccg tcggccggag gcaggtactc cgccagcggg tccgttttcg
     9481 ccagcaccgg gaaggttttc agcgaaattt cccggcgctc aacccgcagc gggctgctga
     9541 acgccagccg cgccacgccc agcccggtca tcaccgcgaa cccgtcaggc aggtgttgca
     9601 gggtgttcac gtcaaacagc ggccgcgcct ccttctggat gaagctgtcg ccgctgatgt
     9661 gctccacgtt ccccgcctcc cggctggccc ggcggcgctc cacgtccacc cggatctccc
     9721 cggtctggcc cgccgcccac tgcgcggact cctggctggc cgcgcgatag aaccagcgga
     9781 tcggcgtgtt atccagtacc gtggttttca caaaatcagc gggcagatcc tgcccgcact
     9841 gaccgaagtc gcccagcgac tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg
     9901 tccccagcgc gttcagcacg tacttcgaca gcagatattt aatctcatcg agcatgatgc
     9961 tcgcgtgcgg ccacctccgg aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa
    10021 acagcatctt ctgcacccgg atcaccgcct catcgtccat cgagccgatg acgtacaggc
    10081 agccgccgcc gttgattatc cccgccacat cgataccgct gtccgtctgc aggacgctca
    10141 ggtcggccac gttctccagc ttggtgatca gccccttcac gtcctttttc agggcctccg
    10201 gcaacgcgta cgccgccgcc agcacctgct gaatgtttgc cccctgcggg aactgctccg
    10261 caatcagctt cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct
    10321 cactcaggtt aaacccggca accagcagat tcttcagggc gtactggtcg agatcgcgga
    10381 acagattcag ctgcgccggt ttccccggcc gcagatccag cagggtgaag tccgggcagt
    10441 gcgcccggaa gacgtgcggc gcccacgcgt cccctttggg gtcgaagtac accacagcgt
    10501 ccccgaaatc gcgtacgcag cgaatgagcg cgttggtggc catcgcgctc ttgccgctcc
    10561 cgggcaggcc gaggatctga atattggtct tgcgccaggt atcacgaggg accagcaccg
    10621 gctggccctg ctcatcctta cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg
    10681 aaaaatcggc cagcgcgtta accggtaccg gctccccgaa atgcaggtgg cggacgtctg
    10741 tcatttctcc ggtccgctgg ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg
    10801 cctcgtcagc ggccgcaaaa tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa
    10861 ggacgccggc gacaagccct ccggcacacc aggctatccc gaacaggtta gccggctgca
    10921 gcaggtaata atctgcgttc gcatagaggt ccgggcgcag gacggtttct ccccagctct
    10981 cgataaacac ggcgacaacc agccccccca gaagggccag accggcagcg ccggtcagcc
    11041 agtacgcgta gttccagaca tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg
    11101 aggccagcac cgaaaatggc acagtccggt tattatcgcg cttatagcga tcggcatact
    11161 cccgccgcgc cttatggctc agccccacaa aacgcattcg gccggcccag agttcagcca
    11221 gaccgaaaaa caggccgata accagaaaaa aaccggccag actgtaatac tcccggtaca
    11281 gccagctgac ccacgggtaa atctgccggg ccagctccgg gatatgccac tgccaggcaa
    11341 gcgaatccgt attaaacatg caatctctct ccttcagacg acgggtttac cggcgtgatg
    11401 cgcagccccc gctgcacaag ccgcggacat gccccaaggc atggcggcgg cggtcacgtc
    11461 cgtggtgatt gcgttcagct ttcaccgcgg aggccagcct ctttccgccc catgcggaaa
    11521 aggcgcagcc ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc
    11581 ccagtgcggt cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga
    11641 ccacccgcaa aatcaggagc agctttgctg cttcgtcagg tgcgtccagc accgtggatt
    11701 tttgtgggtg gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg
    11761 tccagccgga cagagccaga aagaggggga aaagaccgtg cgtcggtggc gtccagccag
    11821 cgtaagcaag gtcttttccc ctgaagtcag ccacgcccca gcggggatga cttgcatttc
    11881 tccgcaagga gaaaaaagca acagagatag gttaacgtaa aatgttcagc gaaaaaccga
    11941 catggttcac ctatcctgat aattgatcgt ca
//
