LOCUS       NZ_CP084500            10060 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 1632 plasmid p1632-3, complete
            sequence.
ACCESSION   NZ_CP084500
VERSION     NZ_CP084500.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21874042
            Assembly: GCF_020405285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 10060)
  AUTHORS   Zhou,Y.
  TITLE     Klebsiella pneumoniae harboring armA rmtB and KPC-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10060)
  AUTHORS   Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2021) Department of Clinical Laboratory Medicine,
            Shanghai Pulmonary Hospital, Yangpu District, Shanghai 200082, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. V2.5; CANU v. V1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:05:03
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,801
            CDSs (total)                      :: 5,677
            Genes (coding)                    :: 5,539
            CDSs (with protein)               :: 5,539
            Genes (RNA)                       :: 124
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 87
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 138
            CDSs (without protein)            :: 138
            Pseudo Genes (ambiguous residues) :: 0 of 138
            Pseudo Genes (frameshifted)       :: 53 of 138
            Pseudo Genes (incomplete)         :: 86 of 138
            Pseudo Genes (internal stop)      :: 15 of 138
            Pseudo Genes (multiple problems)  :: 16 of 138
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084500.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..10060
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="1632"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1632-3"
                     /geo_loc_name="China"
                     /collection_date="2018-11-09"
     gene            complement(join(9725..10060,1..1629))
                     /locus_tag="LDL62_RS28825"
                     /old_locus_tag="LDL62_28825"
     CDS             complement(join(9725..10060,1..1629))
                     /locus_tag="LDL62_RS28825"
                     /old_locus_tag="LDL62_28825"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(3113..3262)
                     /locus_tag="LDL62_RS28830"
                     /old_locus_tag="LDL62_28830"
     CDS             complement(3113..3262)
                     /locus_tag="LDL62_RS28830"
                     /old_locus_tag="LDL62_28830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(3347..3604)
                     /locus_tag="LDL62_RS28835"
                     /old_locus_tag="LDL62_28835"
     CDS             complement(3347..3604)
                     /locus_tag="LDL62_RS28835"
                     /old_locus_tag="LDL62_28835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(3614..5299)
                     /locus_tag="LDL62_RS28840"
                     /old_locus_tag="LDL62_28840"
     CDS             complement(3614..5299)
                     /locus_tag="LDL62_RS28840"
                     /old_locus_tag="LDL62_28840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            5626..5871
                     /locus_tag="LDL62_RS28845"
                     /old_locus_tag="LDL62_28845"
     CDS             5626..5871
                     /locus_tag="LDL62_RS28845"
                     /old_locus_tag="LDL62_28845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6145..6516
                     /gene="tolA"
                     /locus_tag="LDL62_RS28850"
                     /old_locus_tag="LDL62_28850"
     CDS             6145..6516
                     /gene="tolA"
                     /locus_tag="LDL62_RS28850"
                     /old_locus_tag="LDL62_28850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            6513..6878
                     /locus_tag="LDL62_RS28855"
                     /old_locus_tag="LDL62_28855"
     CDS             6513..6878
                     /locus_tag="LDL62_RS28855"
                     /old_locus_tag="LDL62_28855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            7053..7331
                     /locus_tag="LDL62_RS28860"
                     /old_locus_tag="LDL62_28860"
     CDS             7053..7331
                     /locus_tag="LDL62_RS28860"
                     /old_locus_tag="LDL62_28860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            7319..7633
                     /locus_tag="LDL62_RS28865"
                     /old_locus_tag="LDL62_28865"
     CDS             7319..7633
                     /locus_tag="LDL62_RS28865"
                     /old_locus_tag="LDL62_28865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            7797..8225
                     /locus_tag="LDL62_RS28870"
                     /old_locus_tag="LDL62_28870"
     CDS             7797..8225
                     /locus_tag="LDL62_RS28870"
                     /old_locus_tag="LDL62_28870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            8251..8430
                     /locus_tag="LDL62_RS28875"
                     /old_locus_tag="LDL62_28875"
     CDS             8251..8430
                     /locus_tag="LDL62_RS28875"
                     /old_locus_tag="LDL62_28875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(8457..8987)
                     /locus_tag="LDL62_RS28880"
                     /old_locus_tag="LDL62_28880"
     CDS             complement(8457..8987)
                     /locus_tag="LDL62_RS28880"
                     /old_locus_tag="LDL62_28880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(8994..9725)
                     /gene="mobC"
                     /locus_tag="LDL62_RS28885"
                     /old_locus_tag="LDL62_28885"
     CDS             complement(8994..9725)
                     /gene="mobC"
                     /locus_tag="LDL62_RS28885"
                     /old_locus_tag="LDL62_28885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     BGC             1..10060
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          2432..3010
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(8998..9725)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.34e-140"
ORIGIN
        1 cgccgcccac tgcgcggact cctggctggc cgcgcgatag aaccagcgga tcggcgtgtt
       61 atccagtacc gtggttttca caaaatcagc gggcagatcc tgcccgcact gaccgaagtc
      121 gcccagcgac tggtgcgcga gcagcaggtt gcagtcccgg ctgcgcagcg tccccagcgc
      181 gttcagcacg tacttcgaca gcagatattt aatctcatcg agcatgatgc tcgcgtgcgg
      241 ccacctccgg aactcatccc gcgcaatgat gatttgcgcg cagcgggcaa acagcatctt
      301 ctgcacccgg atcaccgcct catcgtccat cgagccgatg acgtacaggc agccgccgcc
      361 gttgattatc cccgccacat cgataccgct gtccgtctgc aggacgctca ggtcggccac
      421 gttctccagc ttggtgatca gccccttcac gtcctttttc agggcctccg gcaacgcgta
      481 cgccgccgcc agcacctgct gaatgtttgc cccctgcggg aactgctccg caatcagctt
      541 cgccgctttc tgctcgctga tgcggtagtg gtccgccacg tcgccggtct cactcaggtt
      601 aaacccggca accagcagat tcttcagggc gtactggtcg agatcgcgga acagattcag
      661 ctgcgccggt ttccccggcc gcagatccag cagggtgaag tccgggcagt gcgcccggaa
      721 gacgtgcggc gcccacgcgt cccctttggg gtcgaagtac accacagcgt ccccgaaatc
      781 gcgtacgcag cgaatgagcg cgttggtggc catcgcgctc ttgccgctcc cgggcaggcc
      841 gaggatctga atattggtct tgcgccaggt atcacgaggg accagcaccg gctggccctg
      901 ctcatcctta cccagaaaca ccgcctgctg tttccgggcc tgctccgtgg aaaaatcggc
      961 cagcgcgtta accggtaccg gctccccgaa atgcaggtgg cggacgtctg tcatttctcc
     1021 ggtccgctgg ctgctgcgcc ggctctcctg attcagtttc gcgttcaccg cctcgtcagc
     1081 ggccgcaaaa tgaccatcaa gcagcaggaa cgccagcagc cgcgccagaa ggacgccggc
     1141 gacaagccct ccggcacacc aggctatccc gaacaggtta gccggctgca gcaggtaata
     1201 atctgcgttc gcatagaggt ccgggcgcag gacggtttct ccccagctct cgataaacac
     1261 ggcgacaacc agccccccca gaagggccag accggcagcg ccggtcagcc agtacgcgta
     1321 gttccagaca tgtcgccgtg ccctgaccat ccgcccgggt gaatccagcg aggccagcac
     1381 cgaaaatggc acagtccggt tattatcgcg cttatagcga tcggcatact cccgccgcgc
     1441 cttatggctc agccccacaa aacgcattcg gccggcccag agttcagcca gaccgaaaaa
     1501 caggccgata accagaaaaa aaccggccag actgtaatac tcccggtaca gccagctgac
     1561 ccacgggtaa atctgccggg ccagctccgg gatatgccac tgccaggcaa gcgaatccgt
     1621 attaaacatg caatctctct ccttcagacg acgggtttac cggcgtgatg cgcagccccc
     1681 gctgcacaag ccgcggacat gccccaaggc atggcggcgg cggtcacgtc cgtggtgatt
     1741 gcgttcagct ttcaccgcgg aggccagcct ctttccgccc catgcggaaa aggcgcagcc
     1801 ccggaggaac aacgagcacg ccccagcgtg tgagaggaaa aggcggcacc ccagtgcggt
     1861 cttttccggt gttccggccc ccgtcagagt tcgccatagc gagcggctga ccacccgcaa
     1921 aatcaggagc agctttgctg cttcgtcagg tgcgtccagc accgtggatt tttgtgggtg
     1981 gtcaggcgtg agcctgacgg gggccgggtg aatttctggc gtggccggcg tccagccgga
     2041 cagagccaga aagaggggaa aagaccgtgc gtcggtggcg tccagccagc gtaagcaagg
     2101 tcttttcccc tgaagtcagc cacgccccag cggggatgac ttgcatttct ccgcaaggag
     2161 aaaaaagcaa cagagatagg ttaacgtaaa atgttcagcg aaaaaccgac atggttcacc
     2221 tatcctgata attgatcgtc agggaataga aagacttaat caggggacaa tatcccacat
     2281 cagcgcgtcc ggtgaccgtc ttgcaggtgc cggggaaatt gcgcctcctc gctcactcgg
     2341 tcgctacgct ccgggcgtga gactgcggcg ggcgctgcgg acacatacaa agttacccac
     2401 agattccgtg gataagcagg ggactaacat gtgaggcaaa acggcagggc cgcgccggtg
     2461 gcgtttttcc ataggctccg ccctcctgcc agagttcaca taaacagacg ctttttcggt
     2521 gcatctgtgg gagccgtgag gctcaaccat gaatctgaca gtacgggcga aacccgacag
     2581 gacttaaaga tccccaccgt ttccggtagg tcgctccctc ttgcgctctc ctgttccgac
     2641 cctgccgttt accggatacc tgtccgccgt tctcccttgc gggaagtgtg gcgctttctc
     2701 atagctcaca cactggtatc tcggctcggt gtaggtcgtt cgctccaagc tgggctgtaa
     2761 gcaagaactc cccgttcagc ccgactgctg cgccttatcc ggtaactgtt cacttgagtc
     2821 caacccggga aagcacgcaa aacgccactg gcagcagcca ttggtaactg ggagttcgca
     2881 gaggatttgt ttagctaaac acgcggttgc tcttgaagtg tgcgccaaag tccggctaca
     2941 ctggaaggac agatttggtt gctgtgctct gcgaaagcca gttaccacgg ttaagcagtt
     3001 ccccaactga cttaaccttc gatcaaacca cctccccagg tggttttttc gtttacaggg
     3061 caaaagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctactgaa
     3121 ccgcgatccc cgtcagttca gaggaggacg atggtgccac cgttccgccc tgaacatccc
     3181 ggatataatt tgcctgacat gcggccagaa gaaagccagc aacaatcaat ataaaaagaa
     3241 aaatagtctt tacctttttc atatactttc cctgtattta aagactccgg cattcatccc
     3301 tgaacaccgg aagtcagtat ttcaaatcat ccttgataat tttttgttac cagttaccgt
     3361 cccgataatc aaaagctata taacaattat ccacatcagg ttttatttga tttttaaaaa
     3421 atggctgtag taaaggaacc cattctttct tcaaatcaaa ctcaccatta ttaatattat
     3481 cctttaaagg cattcccaag ctttcaatga cagaaccatc gtcaccaaag tctttagaat
     3541 actcttcccc tttaaactct tcagttttct tatcaaacca tgtcaaacgt aatttaagcc
     3601 ccatatttac ctctcaaaga tattttttaa tgttccgttt tggatctggc cctttaacct
     3661 gcttacccgt tttgggatca aatgcgccta ggtgttcgcc atcactggca cgataccctt
     3721 caagctcacc gtgctgggaa tcccattcat aaattttacg ttttttatcg ccataccatc
     3781 gggcccgctt accaccacca ccttgtttag gtgttttagg atcacctcgc tttaactctc
     3841 caagtccctt tatgtcttct gttttaggta ctggatgata atcatggccg tagtctttag
     3901 tccccttgcg aggcttgttc ttttcctcat ttaacttatt ttctgcatcc ttcgcttttt
     3961 tctccttttg tttccggctc tccatcgccc cggtaagtgc agccttatct ctggataagg
     4021 attcattcat tgcgtttaaa gatgcctgag ccgcttttaa tttctcattc tgattctgaa
     4081 tatccttatc aagatagcga acctgatcct gccacccgga gccatattca tgaggcgggt
     4141 tataagtcac aaaatcatct ttatttgcct ctgcctcttt taccttttta tcggcttcct
     4201 gaacagcagc ccttgcaccg gggagcgcct tctctgtcga tgcaatgcgg ctgtttaagg
     4261 tcgcaatatt tttatcttcg gcatccagct cagctttcgc tttatcatag tctcttttca
     4321 gcccctcttc cggatgagcg gcgtcccatg cctgctggcg acgcttttct tcctcctgac
     4381 gctgttttac ctgtgccggg gtaagaacat ctgtcaccga cacataaacc ggcttctgtc
     4441 cggtctcttt cgggaaacga ataacggcct cacgggagtt accaccggct gtaaaaccag
     4501 ccggacgtga atcacctttt tcagcaacaa tgccttttgg cggggctttc gctgtcggaa
     4561 cacctttcgg tacgctcacc tgcagagacg ggagacccgg aatcgacaca ctgaataccc
     4621 ccggacgttt tgtcggtttc gcatccacca cagggacact cattggccga cctgcgacaa
     4681 ccgcaatatg ctgccgctca tccttcacca catccacaac ccgttgtctg acacgaaccg
     4741 tcgcctggtc cagtggtaaa gtacttgccg gagtctccgt caccgcatcg gccggtaatg
     4801 acgtcataat ttttgacatc attttcggat catcttttgc aatctcagaa ggcagcacac
     4861 cgtagatcgc aatcccccac agaccaaact taaacggccc cttcagggca gccagcacat
     4921 cagcaacggc ggccgacaac gcatcgccgg aaactgataa agccagtcct tcagcccccg
     4981 gagtagccag ggcaggcaga ccgaaggcca tcgcagtggc cgaagactgt ccgccaccag
     5041 agctaccgct atgattgccg ctgttaccac cattaccacc gggtttactc ccgccgttac
     5101 caaactcccc cggattatag ttatgaatat ctccattcgg cgtatgcgtc gtaccccagc
     5161 ccggcccgct gtttggatcc gtaccaccac ctgatgaagg gccaccttta ccagacgtac
     5221 cgttcacatt cccactggcc tgaccaccat tatgtcccag acctgaatta cccggacctc
     5281 gaccgtctcc accactcata ccgttttcct ctttgacatt taaaacaata aattaaagcc
     5341 aacatactgt acatatacac agtataatta ttaccttact cctcccgttt tcaggtgtca
     5401 atatatgaga ttagttctca tcttcaggca caaaaaagcg cccggagcca cctgtaagca
     5461 gatcgcgtct tttcccgaaa atttctcatg gcaacaaatt ttattattta agacattcat
     5521 cacacttaca tttcagatgg tatagacaca tctgaaagct aactggcatg aggagaggac
     5581 ctgcggttaa actgttcggg aaacctgaga cgcccggagg ccactatgac gcacctgaaa
     5641 ctgactcccg ctgaagaaaa gcgcctggcg gacgcctttg tcgctcaggc actggccatg
     5701 ggcctgaaag ggaacaaaac gctgccaaag gcggtaaaac aacaggtgcg ggaaagggtg
     5761 cttcgtgcca ttcaggacga acgtgaagcc aggcgggcgc aggaaatgcg tcgggagaaa
     5821 tacgaggcta cggaaaccac cttcacctgg cagccacagc gccgccggta aggggtattt
     5881 accataacgt cgcattgccc gcaccgctca gacggtctga gggcgtttcc ggcggctacg
     5941 ggataaaaca ggcaacaaac cacccggaaa accgccggaa acgcgcccgg taaattgtaa
     6001 cccgctgcat gcctatgcac gcaactgcat ggcgggccgt ttggtgtaaa aacgatacga
     6061 taagccgtgt ttatgtaaat gaacgtggcc gtaaagagtg acaaatatac atgtaacatt
     6121 ttttactgac aggtcatcaa cccgatgcgt ttaataccct ttatcctgct ggcattgtgc
     6181 agccccttac tcctgacggc ctgtaagcat tccaccaccc agccgccgaa aatttatact
     6241 ctggataagt catctaaaat cagtagctac gctgcggcca tcaagaccgc tgttgaaatg
     6301 cagatgtttg aaccagaggc ctatgcggga aaaatgtgca ccgtgcggat atccatacaa
     6361 cgtgatggca ctctgaacag cgccattgct gaaggaggag atccgaaact ctgtaaagca
     6421 gcaatatcag ccgttaccca cgccaaaatt ccgccggcac cggatgaaaa tacctggcaa
     6481 atattcaaaa acgcccctct ggatttcaga ccatgaaagc ttttactctg acagtgctgg
     6541 cggccatgtt cctcactccg gtacatgcga taccactaaa acccgtaacc acactgcagc
     6601 caacctaccc ggtacaggcc gcagcaaaac gcatcgaagg tcaggtgacc gccacgtttg
     6661 atattctgga cgatggacga gtggacaacg tgtcgcttac cggctgtcac cttttttttc
     6721 gggaaacgct gacggcaatg aggcactggc tatatcccgc agggcacccg gcaatgcgac
     6781 aggttataaa ttttgaattc agtctgaaaa atgacggctt acaggatccg cgaccagtca
     6841 ccacccaaaa gctcaatgat gatggttgcc gccagtaaca ggtcctttaa cccgcaggct
     6901 gcaatagtcc tggatgatga gtgagcctgt agcagccctg attagtggaa gcgaagctga
     6961 aaaatgggca gcaaaagccg tatttctggc aaatagcgcc gccacggatt aaagtagtca
     7021 tgtgtaatac atatgactac aaaggggctt ttatgtcatc agcaacatcc atcaggctcg
     7081 atgaagaact taaggaccgg ctgaaaacgc tggccgatga ccggcaccgc tcagcgcacg
     7141 cgctaatgct ggaggcaatc accgaataca tcgaccggga agagaagcgc agccagtacc
     7201 tgcgggacgg acaggccgca tggcagcact atcaggaaac gggtctgcac cttactgcgg
     7261 aagaagcgga agcctggata agtacctggg gaactgagaa cgagcaggat gctccgccat
     7321 gccacaggta acgatttccg cgctggcaca gcgcgattta caacgccttc aggactttct
     7381 caaaaccaaa aatcggctgg cagccagaaa aggaggtgag gtgatcgtcc gggctatcca
     7441 gcaactgaaa acattgccag acattggccg cccggtgccg tttctgccgc tggaatataa
     7501 ggaactggtg atcgggtttg gcgacagtgg ctatgtgatg ctctaccgcc acgaccggga
     7561 aatggaccag attgtgatcg tcaccgtcag gcatcagaaa gaatccgggt atccgggggc
     7621 agacagcctc taataaccga tgacgggggc gcaattcatt gcgtggccgt cgccggtttg
     7681 acaagcgcag cgcgtcagaa aaccacatcc tgaacggaaa aacgggtaaa attcagcgat
     7741 acatttaccg tgagaaatta cacacctttc acacccgata aaatcagggg tatacagtgg
     7801 cgaaaacatt gctcaacctt tcacaggcag cacaagccgc gggcattacc agaagaacgc
     7861 tatacaatca tgtaaaacag ggaaaggtta ccgtgtcacg tgacgggaaa aacaaccctg
     7921 tcgtggatgt gtcagaactg atccgtgtat acggaaatgt gaatatccct gagaaacaaa
     7981 ttcccggcat ttcacaccgt gaaaatacac aaaaaaattt cccacaggaa cagcttctgg
     8041 caatgcaaaa ggaactggcc gatctccggc aggctgtcac cctgatgctg gaagataaaa
     8101 catcccggga agaagaacgc cgccagcacg atgatgagcg ccggaaactc caggccgaag
     8161 tggacagatt gacaacagaa ctgacacaga agaaaaaacg cttctggtcc ggctggttta
     8221 gctgagaact taccgaaggg aatggagaac atgacgaagc aggaaaaaac tgccctcaat
     8281 atggctaaat tcattaagga tcagtcgctg ctgttgctgg aaaagctgaa cgagctggac
     8341 atggataccc cggccgacat gtgcgaacgg cttcacgagg atgcggaaac actctggcag
     8401 gtgatgcggg ataagctcgg ggaagaataa cagcaccggc catgccggtg ctggcgttat
     8461 tttttgccga acggccacca ccggcgggcg ggtttgtcct ccggcagttc aggcaccggc
     8521 acgttaaaat agcggctgtt atattcccgc atcagctccg ctttcagctt ctcgttcacc
     8581 gcgctttccc cgtgccgctc gctgaccacc tcaatcatcg cgttaatctg ctccggtgag
     8641 cacttcccgg catcaaccac ctgcacaccg ttctcgatgg cgaaccgctc cacctttcga
     8701 tagaggtaat cacacagctt cattcctgtg ggtttgtccc gttcccattg cggattttca
     8761 ccggtgaaaa agtacgtggc tttttcgtcg ctgttgagcc gctcgtcata catcccgatg
     8821 gatttcatca tcgccagatg ctgggcccgg tcgacaaaac agaaaacata cgccccggtc
     8881 tgccaggcga gcaccaccat gtcttccaga tgctcgctgg ttggcataat gcagcgctcg
     8941 ccgtctttgt cttccacgtc ctcaataaat accatgtaca gcgccataat ccctcactcc
     9001 gttgtcataa atttaaagca cgcgaacagc ctctcccggt ccagctccac cgttttgcgg
     9061 gcctcgccgg tctgtggatt cacgatttca tccaccgcca gaatcgcccg ctgtacccgg
     9121 acaagcgtcg gccggtcagg gcagatgtac cagatttcat cgatgccgtt cgcccggcgg
     9181 ttgaacagat acccggacac cacttcctga taccgcctgc ggctcttgac ggtgcgctct
     9241 acctcaaatg ccaccgttct gccgtccagc tccgccagcg cgtccggcac tttcatccct
     9301 ttcagggcca tacggtgcag gtaccgccag ccggtggccc cccggccctc catggagagg
     9361 cgcagggact gcacggccag ccggtgcggc agctgcgccg ccgacaccct tgacggctga
     9421 aacggaataa cgtccgtcag cggctcgtcc tcgtcgaacg agaacgccac cccgtgcggc
     9481 gtcagccccc acacgctgac ctgacggccg tcaaccgggt atttcgcccg gaccagcagc
     9541 cctttcgcca ccagactgtc caggaagcgg taaaccggct gtaccgccgc aatccccagc
     9601 cgtcgcgcaa ccagctccgc cgtggaacac agctcttccc gcagccagcg cagggtcagc
     9661 cgtgttttct ccgcgttccg cgcctgccgc ttcgcgtggc tgacgttgta ctgctccagt
     9721 gtcatcagta aagccccgca aaatcatcat ccgcttttgg tccgccgccg tcggccggag
     9781 gcaggtactc cgccagcggg tccgttttcg ccagcaccgg gaaggttttc agcgaaattt
     9841 cccggcgctc aacccgcagc gggctgctga acgccagccg cgccacgccc agcccggtca
     9901 tcaccgcgaa cccgtcaggc aggtgttgca gggtgttcac gtcaaacagc ggccgcgcct
     9961 ccttctggat gaagctgtcg ccgctgatgt gctccacgtt ccccgcctcc cggctggccc
    10021 ggcggcgctc cacgtccacc cggatctccc cggtctggcc
//
