LOCUS       NZ_CP084496            11970 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain 1864 plasmid p1864-4, complete
            sequence.
ACCESSION   NZ_CP084496
VERSION     NZ_CP084496.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21874043
            Assembly: GCF_020405265.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Zhou,Y.
  TITLE     Klebsiella pneumoniae harboring armA rmtB and KPC-2
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11970)
  AUTHORS   Zhou,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (29-SEP-2021) Department of Clinical Laboratory Medicine,
            Shanghai Pulmonary Hospital, Yangpu District, Shanghai 200082, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Flye v. V2.5; CANU v. V1.7.1
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology             :: PacBio Sequel
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:20:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,942
            CDSs (total)                      :: 5,820
            Genes (coding)                    :: 5,653
            CDSs (with protein)               :: 5,653
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 167
            CDSs (without protein)            :: 167
            Pseudo Genes (ambiguous residues) :: 0 of 167
            Pseudo Genes (frameshifted)       :: 62 of 167
            Pseudo Genes (incomplete)         :: 110 of 167
            Pseudo Genes (internal stop)      :: 18 of 167
            Pseudo Genes (multiple problems)  :: 22 of 167
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084496.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="1864"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="p1864-4"
                     /geo_loc_name="China"
                     /collection_date="2018-11-09"
     gene            279..524
                     /locus_tag="LDL64_RS29580"
                     /old_locus_tag="LDL64_29580"
     CDS             279..524
                     /locus_tag="LDL64_RS29580"
                     /old_locus_tag="LDL64_29580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            798..1169
                     /gene="tolA"
                     /locus_tag="LDL64_RS29585"
                     /old_locus_tag="LDL64_29585"
     CDS             798..1169
                     /gene="tolA"
                     /locus_tag="LDL64_RS29585"
                     /old_locus_tag="LDL64_29585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237044.1"
                     /GO_component="GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA]"
                     /GO_function="GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="WP_001237044.1"
                     /translation="MRLIPFILLALCSPLLLTACKHSTTQPPKIYTLDKSSKISSYAAA
                     IKTAVEMQMFEPEAYAGKMCTVRISIQRDGTLNSAIAEGGDPKLCKAAISAVTHAKIPP
                     APDENTWQIFKNAPLDFRP"
                     /name="tolA"
     gene            1166..1531
                     /locus_tag="LDL64_RS29590"
                     /old_locus_tag="LDL64_29590"
     CDS             1166..1531
                     /locus_tag="LDL64_RS29590"
                     /old_locus_tag="LDL64_29590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /GO_function="GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0006810 - transport [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="WP_072354022.1"
                     /translation="MKAFTLTVLAAMFLTPVHAIPLKPVTTLQPTYPVQAAAKRIEGQV
                     TATFDILDDGRVDNVSLTGCHLFFRETLTAMRHWLYPAGHPAMRQVINFEFSLKNDGLQ
                     DPRPVTTQKLNDDGCRQ"
                     /name="TonB family protein"
     gene            1706..1984
                     /locus_tag="LDL64_RS29595"
                     /old_locus_tag="LDL64_29595"
     CDS             1706..1984
                     /locus_tag="LDL64_RS29595"
                     /old_locus_tag="LDL64_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014542884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="WP_000093040.1"
                     /translation="MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEK
                     RSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWGTENEQDAPPCHR"
                     /name="CopG family ribbon-helix-helix protein"
     gene            1972..2286
                     /locus_tag="LDL64_RS29600"
                     /old_locus_tag="LDL64_29600"
     CDS             1972..2286
                     /locus_tag="LDL64_RS29600"
                     /old_locus_tag="LDL64_29600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136729.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]; GO:0009432 - SOS response [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_071177730.1"
                     /translation="MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKT
                     LPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDREMDQIVIVTVRHQKESGYPGADSL
                     "
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            2450..2878
                     /locus_tag="LDL64_RS29605"
                     /old_locus_tag="LDL64_29605"
     CDS             2450..2878
                     /locus_tag="LDL64_RS29605"
                     /old_locus_tag="LDL64_29605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_001140599.1"
                     /translation="MAKTLLNLSQAAQAAGITRRTLYNHVKQGKVTVSRDGKNNPVVDV
                     SELIRVYGNVNIPEKQIPGISHRENTQKNFPQEQLLAMQKELADLRQAVTLMLEDKTSR
                     EEERRQHDDERRKLQAEVDRLTTELTQKKKRFWSGWFS"
                     /name="hypothetical protein"
     gene            2904..3083
                     /locus_tag="LDL64_RS29610"
                     /old_locus_tag="LDL64_29610"
     CDS             2904..3083
                     /locus_tag="LDL64_RS29610"
                     /old_locus_tag="LDL64_29610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_000165970.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            complement(3110..3640)
                     /locus_tag="LDL64_RS29615"
                     /old_locus_tag="LDL64_29615"
     CDS             complement(3110..3640)
                     /locus_tag="LDL64_RS29615"
                     /old_locus_tag="LDL64_29615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071177729.1"
                     /translation="MALYMVFIEDVEDKDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGENPQWERDKPTGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQINAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(3647..4378)
                     /gene="mobC"
                     /locus_tag="LDL64_RS29620"
                     /old_locus_tag="LDL64_29620"
     CDS             complement(3647..4378)
                     /gene="mobC"
                     /locus_tag="LDL64_RS29620"
                     /old_locus_tag="LDL64_29620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071177728.1"
                     /translation="MTLEQYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLSMEGRGATGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRPTLVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            complement(4378..6342)
                     /locus_tag="LDL64_RS29625"
                     /old_locus_tag="LDL64_29625"
     CDS             complement(4378..6342)
                     /locus_tag="LDL64_RS29625"
                     /old_locus_tag="LDL64_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_071177727.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKARREYADRYKRDNNRTVPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSAMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVERREISLKTFPVLAKTDPLAEY
                     LPPADGGGPKADDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            complement(7826..7975)
                     /locus_tag="LDL64_RS29630"
                     /old_locus_tag="LDL64_29630"
     CDS             complement(7826..7975)
                     /locus_tag="LDL64_RS29630"
                     /old_locus_tag="LDL64_29630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            complement(8060..8317)
                     /locus_tag="LDL64_RS29635"
                     /old_locus_tag="LDL64_29635"
     CDS             complement(8060..8317)
                     /locus_tag="LDL64_RS29635"
                     /old_locus_tag="LDL64_29635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     gene            complement(8327..10012)
                     /locus_tag="LDL64_RS29640"
                     /old_locus_tag="LDL64_29640"
     CDS             complement(8327..10012)
                     /locus_tag="LDL64_RS29640"
                     /old_locus_tag="LDL64_29640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_032440457.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARAAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            complement(10117..11604)
                     /gene="ltrA"
                     /locus_tag="LDL64_RS29645"
                     /old_locus_tag="LDL64_29645"
     CDS             complement(10117..11604)
                     /gene="ltrA"
                     /locus_tag="LDL64_RS29645"
                     /old_locus_tag="LDL64_29645"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226014.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_004178082.1"
                     /translation="MMYGEEKSDSLIVAAKQANNPKGAESVERRSGAKGNAEQPHMRRT
                     QSRESMSQRLSRVREAAKQRKKERFTALFHLLTVEALEAAFLSLSRKAAAGVDGIRWMD
                     YAGNMKNNITDLHRRLHQGSYRAQPGRRHYIPKADGKQRPLGIASLEDKIVQYALVKIL
                     NAVYENDFMGFSYGFRPGRSQHDALDALATGLVRTNVNWVLDADISQFFDRVSHEWLIR
                     FTEHRIGDRRVIRLIRKWLTAGTSEEGQWRATEEGTPQGAVISPLLANIYLHYVFDLWA
                     HQWRRRYATGNVVMVRYADDIVIGFDKRYDARRFRIAMQRRLREFGLTVHPEKTRLMEF
                     GRFAAENRAIRGKGKPETFNFLGFTHISGKDRNGRFMLIRKTRRDRMTATLKAIKDGLR
                     RRWHYSIPEQGKWLRRVVQGYLNYHSVPGNFPTMQKFRTHVTNLWRRALRRRSQKDDTT
                     WTKANKLAAAWLPRVRVLHPWPVERFTARHPRQEPGA"
                     /name="ltrA"
     BGC             3327..11970
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          7145..7723
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          complement(3651..4378)
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.416"
                     /coverage="100.0"
                     /evalue="1.35e-140"
ORIGIN
        1 tgtacatata cacagtataa ttattacctt actcctcccg ttttcaggtg tcaatatatg
       61 agattagttc tcatcttcag gcacaaaaaa gcgcccggag ccacctgtaa gcagatcgcg
      121 tcttttcccg aaaatttctc atggcaacaa attttattat ttaagacatt catcacactt
      181 acatttcaga tggtatagac acatctgaaa gctaactggc atgaggagag gacctgcggt
      241 taaactgttc gggaaacctg agacgcccgg aggccactat gacgcacctg aaactgactc
      301 ccgctgaaga aaagcgcctg gcggacgcct ttgtcgctca ggcactggcc atgggcctga
      361 aagggaacaa aacgctgcca aaggcggtaa aacaacaggt gcgggaaagg gtgcttcgtg
      421 ccattcagga cgaacgtgaa gccaggcggg cgcaggaaat gcgtcgggag aaatacgagg
      481 ctacggaaac caccttcacc tggcagccac agcgccgccg gtaaggggta tttaccataa
      541 cgtcgcattg cccgcaccgc tcagacggtc tgagggcgtt tccggcggct acgggataaa
      601 acaggcaaca aaccacccgg aaaaccgccg gaaacgcgcc cggtaaattg taacccgctg
      661 catgcctatg cacgcaactg catggcgggc cgtttggtgt aaaaacgata cgataagccg
      721 tgtttatgta aatgaacgtg gccgtaaaga gtgacaaata tacatgtaac attttttact
      781 gacaggtcat caacccgatg cgtttaatac cctttatcct gctggcattg tgcagcccct
      841 tactcctgac ggcctgtaag cattccacca cccagccgcc gaaaatttat actctggata
      901 agtcatctaa aatcagtagc tacgctgcgg ccatcaagac cgctgttgaa atgcagatgt
      961 ttgaaccaga ggcctatgcg ggaaaaatgt gcaccgtgcg gatatccata caacgtgatg
     1021 gcactctgaa cagcgccatt gctgaaggag gagatccgaa actctgtaaa gcagcaatat
     1081 cagccgttac ccacgccaaa attccgccgg caccggatga aaatacctgg caaatattca
     1141 aaaacgcccc tctggatttc agaccatgaa agcttttact ctgacagtgc tggcggccat
     1201 gttcctcact ccggtacatg cgataccact aaaacccgta accacactgc agccaaccta
     1261 cccggtacag gccgcagcaa aacgcatcga aggtcaggtg accgccacgt ttgatattct
     1321 ggacgatgga cgagtggaca acgtgtcgct taccggctgt cacctttttt ttcgggaaac
     1381 gctgacggca atgaggcact ggctatatcc cgcagggcac ccggcaatgc gacaggttat
     1441 aaattttgaa ttcagtctga aaaatgacgg cttacaggat ccgcgaccag tcaccaccca
     1501 aaagctcaat gatgatggtt gccgccagta acaggtcctt taacccgcag gctgcaatag
     1561 tcctggatga tgagtgagcc tgtagcagcc ctgattagtg gaagcgaagc tgaaaaatgg
     1621 gcagcaaaag ccgtatttct ggcaaatagc gccgccacgg attaaagtag tcatgtgtaa
     1681 tacatatgac tacaaagggg cttttatgtc atcagcaaca tccatcaggc tcgatgaaga
     1741 acttaaggac cggctgaaaa cgctggccga tgaccggcac cgctcagcgc acgcgctaat
     1801 gctggaggca atcaccgaat acatcgaccg ggaagagaag cgcagccagt acctgcggga
     1861 cggacaggcc gcatggcagc actatcagga aacgggtctg caccttactg cggaagaagc
     1921 ggaagcctgg ataagtacct ggggaactga gaacgagcag gatgctccgc catgccacag
     1981 gtaacgattt ccgcgctggc acagcgcgat ttacaacgcc ttcaggactt tctcaaaacc
     2041 aaaaatcggc tggcagccag aaaaggaggt gaggtgatcg tccgggctat ccagcaactg
     2101 aaaacattgc cagacattgg ccgcccggtg ccgtttctgc cgctggaata taaggaactg
     2161 gtgatcgggt ttggcgacag tggctatgtg atgctctacc gccacgaccg ggaaatggac
     2221 cagattgtga tcgtcaccgt caggcatcag aaagaatccg ggtatccggg ggcagacagc
     2281 ctctaataac cgatgacggg ggcgcaattc attgcgtggc cgtcgccggt ttgacaagcg
     2341 cagcgcgtca gaaaaccaca tcctgaacgg aaaaacgggt aaaattcagc gatacattta
     2401 ccgtgagaaa ttacacacct ttcacacccg ataaaatcag gggtatacag tggcgaaaac
     2461 attgctcaac ctttcacagg cagcacaagc cgcgggcatt accagaagaa cgctatacaa
     2521 tcatgtaaaa cagggaaagg ttaccgtgtc acgtgacggg aaaaacaacc ctgtcgtgga
     2581 tgtgtcagaa ctgatccgtg tatacggaaa tgtgaatatc cctgagaaac aaattcccgg
     2641 catttcacac cgtgaaaata cacaaaaaaa tttcccacag gaacagcttc tggcaatgca
     2701 aaaggaactg gccgatctcc ggcaggctgt caccctgatg ctggaagata aaacatcccg
     2761 ggaagaagaa cgccgccagc acgatgatga gcgccggaaa ctccaggccg aagtggacag
     2821 attgacaaca gaactgacac agaagaaaaa acgcttctgg tccggctggt ttagctgaga
     2881 acttaccgaa gggaatggag aacatgacga agcaggaaaa aactgccctc aatatggcta
     2941 aattcattaa ggatcagtcg ctgctgttgc tggaaaagct gaacgagctg gacatggata
     3001 ccccggccga catgtgcgaa cggcttcacg aggatgcgga aacactctgg caggtgatgc
     3061 gggataagct cggggaagaa taacagcacc ggccatgccg gtgctggcgt tattttttgc
     3121 cgaacggcca ccaccggcgg gcgggtttgt cctccggcag ttcaggcacc ggcacgttaa
     3181 aatagcggct gttatattcc cgcatcagct ccgctttcag cttctcgttc accgcgcttt
     3241 ccccgtgccg ctcgctgacc acctcaatca tcgcgttaat ctgctccggt gagcacttcc
     3301 cggcatcaac cacctgcaca ccgttctcga tggcgaaccg ctccaccttt cgatagaggt
     3361 aatcacacag cttcattcct gtgggtttgt cccgttccca ttgcggattt tcaccggtga
     3421 aaaagtacgt ggctttttcg tcgctgttga gccgctcgtc atacatcccg atggatttca
     3481 tcatcgccag atgctgggcc cggtcgacaa aacagaaaac atacgccccg gtctgccagg
     3541 cgagcaccac catgtcttcc agatgctcgc tggttggcat aatgcagcgc tcgccgtctt
     3601 tgtcttccac gtcctcaata aataccatgt acagcgccat aatccctcac tccgttgtca
     3661 taaatttaaa gcacgcgaac agcctctccc ggtccagctc caccgttttg cgggcctcgc
     3721 cggtctgtgg attcacgatt tcatccaccg ccagaatcgc ccgctgtacc cggacaagcg
     3781 tcggccggtc agggcagatg taccagattt catcgatgcc gttcgcccgg cggttgaaca
     3841 gatacccgga caccacttcc tgataccgcc tgcggctctt gacggtgcgc tctacctcaa
     3901 atgccaccgt tctgccgtcc agctccgcca gcgcgtccgg cactttcatc cctttcaggg
     3961 ccatacggtg caggtaccgc cagccggtgg ccccccggcc ctccatggag aggcgcaggg
     4021 actgcacggc cagccggtgc ggcagctgcg ccgccgacac ccttgacggc tgaaacggaa
     4081 taacgtccgt cagcggctcg tcctcgtcga acgagaacgc caccccgtgc ggcgtcagcc
     4141 cccacacgct gacctgacgg ccgtcaaccg ggtatttcgc ccggaccagc agccctttcg
     4201 ccaccagact gtccaggaag cggtaaaccg gctgtaccgc cgcaatcccc agccgtcgcg
     4261 caaccagctc cgccgtggaa cacagctctt cccgcagcca gcgcagggtc agccgtgttt
     4321 tctccgcgtt ccgcgcctgc cgcttcgcgt ggctgacgtt gtactgctcc agtgtcatca
     4381 gtaaagcccc gcaaaatcat catccgcttt tggtccgccg ccgtcggccg gaggcaggta
     4441 ctccgccagc gggtccgttt tcgccagcac cgggaaggtt ttcagcgaaa tttcccggcg
     4501 ctcaacccgc agcgggctgc tgaacgccag ccgcgccacg cccagcccgg tcatcaccgc
     4561 gaacccgtca ggcaggtgtt gcagggtgtt cacgtcaaac agcggccgcg cctccttctg
     4621 gatgaagctg tcgccgctga tgtgctccac gttccccgcc tcccggctgg cccggcggcg
     4681 ctccacgtcc acccggatct ccccggtctg gcccgccgcc cactgcgcgg actcctggct
     4741 ggccgcgcga tagaaccagc ggatcggcgt gttatccagt accgtggttt tcacaaaatc
     4801 agcgggcaga tcctgcccgc actgaccgaa gtcgcccagc gactggtgcg cgagcagcag
     4861 gttgcagtcc cggctgcgca gcgtccccag cgcgttcagc acgtacttcg acagcagata
     4921 tttaatctca tcgagcatga tgctcgcgtg cggccacctc cggaactcat cccgcgcaat
     4981 gatgatttgc gcgcagcggg caaacagcat cttctgcacc cggatcaccg cctcatcgtc
     5041 catcgagccg atgacgtaca ggcagccgcc gccgttgatt atccccgcca catcgatacc
     5101 gctgtccgtc tgcaggacgc tcaggtcggc cacgttctcc agcttggtga tcagcccctt
     5161 cacgtccttt ttcagggcct ccggcaacgc gtacgccgcc gccagcacct gctgaatgtt
     5221 tgccccctgc gggaactgct ccgcaatcag cttcgccgct ttctgctcgc tgatgcggta
     5281 gtggtccgcc acgtcgccgg tctcactcag gttaaacccg gcaaccagca gattcttcag
     5341 ggcgtactgg tcgagatcgc ggaacagatt cagctgcgcc ggtttccccg gccgcagatc
     5401 cagcagggtg aagtccgggc agtgcgcccg gaagacgtgc ggcgcccacg cgtccccttt
     5461 ggggtcgaag tacaccacag cgtccccgaa atcgcgtacg cagcgaatga gcgcgttggt
     5521 ggccatcgcg ctcttgccgc tcccgggcag gccgaggatc tgaatattgg tcttgcgcca
     5581 ggtatcacga gggaccagca ccggctggcc ctgctcatcc ttacccagaa acaccgcctg
     5641 ctgtttccgg gcctgctccg tggaaaaatc ggccagcgcg ttaaccggta ccggctcccc
     5701 gaaatgcagg tggcggacgt ctgtcatttc tccggtccgc tggctgctgc gccggctctc
     5761 ctgattcagt ttcgcgttca ccgcctcgtc agcggccgca aaatgaccat caagcagcag
     5821 gaacgccagc agccgcgcca gaaggacgcc ggcgacaagc cctccggcac accaggctat
     5881 cccgaacagg ttagccggct gcagcaggta ataatctgcg ttcgcataga ggtccgggcg
     5941 caggacggtt tctccccagc tctcgataaa cacggcgaca accagccccc ccagaagggc
     6001 cagaccggca gcgccggtca gccagtacgc gtagttccag acatgtcgcc gtgccctgac
     6061 catccgcccg ggtgaatcca gcgaggccag caccgaaaat ggcacagtcc ggttattatc
     6121 gcgcttatag cgatcggcat actcccgccg cgccttatgg ctcagcccca caaaacgcat
     6181 tcggccggcc cagagttcag ccagaccgaa aaacaggccg ataaccagaa aaaaaccggc
     6241 cagactgtaa tactcccggt acagccagct gacccacggg taaatctgcc gggccagctc
     6301 cgggatatgc cactgccagg caagcgaatc cgtattaaac atgcaatctc tctccttcag
     6361 acgacgggtt taccggcgtg atgcgcagcc cccgctgcac aagccgcgga catgccccaa
     6421 ggcatggcgg cggcggtcac gtccgtggtg attgcgttca gctttcaccg cggaggccag
     6481 cctctttccg ccccatgcgg aaaaggcgca gccccggagg aacaacgagc acgccccagc
     6541 gtgtgagagg aaaaggcggc accccagtgc ggtcttttcc ggtgttccgg cccccgtcag
     6601 agttcgccat agcgagcggc tgaccacccg caaaatcagg agcagctttg ctgcttcgtc
     6661 aggtgcgtcc agcaccgtgg atttttgtgg gtggtcaggc gtgagcctga cgggggccgg
     6721 gtgaatttct ggcgtggccg gcgtccagcc ggacagagcc agaaagaggg gaaaagaccg
     6781 tgcgtcggtg gcgtccagcc agcgtaagca aggtcttttc ccctgaagtc agccacgccc
     6841 cagcggggat gacttgcatt tctccgcaag gagaaaaaag caacagagat aggttaacgt
     6901 aaaatgttca gcgaaaaacc gacatggttc acctatcctg ataattgatc gtcagggaat
     6961 agaaagactt aatcagggga caatatccca catcagcgcg tccggtgacc gtcttgcagg
     7021 tgccggggaa attgcgcctc ctcgctcact cggtcgctac gctccgggcg tgagactgcg
     7081 gcgggcgctg cggacacata caaagttacc cacagattcc gtggataagc aggggactaa
     7141 catgtgaggc aaaacggcag ggccgcgccg gtggcgtttt tccataggct ccgccctcct
     7201 gccagagttc acataaacag acgctttttc ggtgcatctg tgggagccgt gaggctcaac
     7261 catgaatctg acagtacggg cgaaacccga caggacttaa agatccccac cgtttccggt
     7321 aggtcgctcc ctcttgcgct ctcctgttcc gaccctgccg tttaccggat acctgtccgc
     7381 cgttctccct tgcgggaagt gtggcgcttt ctcatagctc acacactggt atctcggctc
     7441 ggtgtaggtc gttcgctcca agctgggctg taagcaagaa ctccccgttc agcccgactg
     7501 ctgcgcctta tccggtaact gttcacttga gtccaacccg ggaaagcacg caaaacgcca
     7561 ctggcagcag ccattggtaa ctgggagttc gcagaggatt tgtttagcta aacacgcggt
     7621 tgctcttgaa gtgtgcgcca aagtccggct acactggaag gacagatttg gttgctgtgc
     7681 tctgcgaaag ccagttacca cggttaagca gttccccaac tgacttaacc ttcgatcaaa
     7741 ccacctcccc aggtggtttt ttcgtttaca gggcaaaaga ttacgcgcag aaaaaaagga
     7801 tctcaagaag atcctttgat cttttctact gaaccgcgat ccccgtcagt tcagaggagg
     7861 acgatggtgc caccgttccg ccctgaacat cccggatata atttgcctga catgcggcca
     7921 gaagaaagcc agcaacaatc aatataaaaa gaaaaatagt ctttaccttt ttcatatact
     7981 ttccctgtat ttaaagactc cggcattcat ccctgaacac cggaagtcag tatttcaaat
     8041 catccttgat aattttttgt taccagttac cgtcccgata atcaaaagct atataacaat
     8101 tatccacatc aggttttatt tgatttttaa aaaatggctg tagtaaagga acccattctt
     8161 tcttcaaatc aaactcacca ttattaatat tatcctttaa aggcattccc aagctttcaa
     8221 tgacagaacc atcgtcacca aagtctttag aatactcttc ccctttaaac tcttcagttt
     8281 tcttatcaaa ccatgtcaaa cgtaatttaa gccccatatt tacctctcaa agatattttt
     8341 taatgttccg ttttggatct ggccctttaa cctgcttacc cgttttggga tcaaatgcgc
     8401 ctaggtgttc gccatcactg gcacgatacc cttcaagctc accgtgctgg gaatcccatt
     8461 cataaatttt acgtttttta tcgccatacc atcgggcccg cttaccacca ccaccttgtt
     8521 taggtgtttt aggatcacct cgctttaact ctccaagtcc ctttatgtct tctgttttag
     8581 gtactggatg ataatcatgg ccgtagtctt tagtcccctt gcgaggcttg ttcttttcct
     8641 catttaactt attttctgca tccttcgctt ttttctcctt ttgtttccgg ctctccatcg
     8701 ccccggtaag tgcagcctta tctctggata aggattcatt cattgcgttt aaagatgcct
     8761 gagccgcttt taatttctca ttctgattct gaatatcctt atcaagatag cgaacctgat
     8821 cctgccaccc ggagccatat tcatgaggcg ggttataagt cacaaaatca tctttatttg
     8881 cctctgcctc ttttaccttt ttatcggctt cctgaacagc agcccttgca ccggggagcg
     8941 ccttctctgt cgatgcaatg cggctgttta aggtcgcaat atttttatct tcggcatcca
     9001 gctcagcttt cgctttatca tagtctcttt tcagcccctc ttccggatga gcggcgtccc
     9061 atgcctgctg gcgacgcttt tcttcctcct gacgctgttt tacctgtgcc ggggtaagaa
     9121 catctgtcac cgacacataa accggcttct gtccggtctc tttcgggaaa cgaataacgg
     9181 cctcacggga gttaccaccg gctgtaaaac cagccggacg tgaatcacct ttttcagcaa
     9241 caatgccttt tggcggggct ttcgctgtcg gaacaccttt cggtacgctc acctgcagag
     9301 acgggagacc cggaatcgac acactgaata cccccggacg ttttgtcggt ttcgcatcca
     9361 ccacagggac actcattggc cgacctgcga caaccgcaat atgctgccgc tcatccttca
     9421 ccacatccac aacccgttgt ctgacacgaa ccgtcgcctg gtccagtggt aaagtacttg
     9481 ccggagtctc cgtcaccgca tcggccggta atgacgtcat aatttttgac atcattttcg
     9541 gatcatcttt tgcaatctca gaaggcagca caccgtagat cgcaatcccc cacagaccaa
     9601 acttaaacgg ccccttcagg gcagccagca catcagcaac ggcggccgac aacgcatcgc
     9661 cggaaactga taaagccagt ccttcagccc ccggagtagc cagggcaggc agaccgaagg
     9721 ccatcgcagt ggccgaagac tgtccgccac cagagctacc gctatgattg ccgctgttac
     9781 caccattacc accgggttta ctcccgccgt taccaaactc ccccggatta tagttatgaa
     9841 tatctccatt cggcgtatgc gtcgtacccc agcccggccc gctgtttgga tccgtaccac
     9901 cacctgatga agggccacct ttaccagacg taccgttcac attcccactg gcctgaccac
     9961 cattatgtcc cagacctgaa ttacccggac ctcgaccgtc tccaccactc ataccgtttt
    10021 cctctttgac atttaaaaca ataaattaaa gccaacatgt cgcggtaggg atacccgtta
    10081 ccggataccc cccgcacaga tcccggcgtg cgcgatttac gcaccgggct cctgcctcgg
    10141 gtgtctggcg gtgaaccgct ccacaggcca tggatgaaga acccgaaccc ttggtagcca
    10201 tgcggctgcc agtttgtttg ctttcgtcca ggtcgtatca tccttctggc tcctgcgcct
    10261 gagcgcccgg cgccagaggt ttgttacgtg tgtcctgaac ttctgcatgg tggggaagtt
    10321 gcccggtacc gagtgatagt tcaggtatcc ctgaaccact ctcctgagcc attttccctg
    10381 ttcggggatt gagtaatgcc agcgccttcg cagaccgtct ttgatggctt tcagagttgc
    10441 cgtcatccga tcccggcggg tctttcgtat cagcatgaac ctgccgttgc gatctttccc
    10501 gctgatgtgc gtgaacccga ggaagttgaa cgtttctggt ttgccttttc ccctgatggc
    10561 acggttttcg gcagcgaagc ggccgaactc catcagacgg gttttctccg ggtgaaccgt
    10621 gagtccgaac tccctcagtc tgcgctgcat ggctatacgg aagcgccggg catcgtatcg
    10681 tttgtcgaac ccgatgacga tgtcatcggc gtatctgacc attaccacat tgcctgtggc
    10741 atagcgacgt cgccactgat gcgcccacag atcgaagacg tagtggaggt atatgtttgc
    10801 cagcagcggt gagatgaccg caccctgtgg ggtgccttcc tccgttgctc gccattgacc
    10861 ctcctccgac gtcccggctg tgagccactt acgtatgagc ctgattaccc tccggtcgcc
    10921 gatccgatgc tctgtgaacc tgatcagcca ttcgtggctc accctgtcga agaactgact
    10981 gatgtcggca tccagtaccc agtttacgtt agtgcgtacc agccctgtgg ccagtgcgtc
    11041 cagtgcatcg tgctggcttc gcccgggtct gaacccgtat gagaacccca taaagtcgtt
    11101 ttcatagact gcgttcagga ttttcaccag cgcatactgg acgatcttgt cctccagcga
    11161 ggcgatgccg agcgggcgtt gttttccatc cgcttttggg atgtagtgac gcctgccggg
    11221 ctgcgccctg tagctgccct gatgtagcct ccggtgcaga tctgttatgt tgttcttcat
    11281 gtttccggcg tagtccatcc acctgatgcc atccactccg gcggccgctt tcctgctcag
    11341 ggagaggaat gcggcttcca gtgcttcgac tgtcagcagg tggaacaatg ctgtaaaccg
    11401 ttctttcttc cgctgcttcg cagcttcccg cacgcgtgac agcctctgtg acatgctttc
    11461 ccggctctgt gtccggcgca tgtgtggctg ttccgcgttc cccttggccc cgctccttcg
    11521 ctccactgac tccgctcctt tcgggttgtt cgcctgcttc gccgctacta tgagcgagtc
    11581 cgacttctcc tctccgtaca tcaccggcta tgactcctcg tcttcccggt gcgggccatc
    11641 tccgacactg gcagatggtc agaggggaga tctcccggtt cccgcgtaga gatcgtattg
    11701 acatgccagg gtctcagacc ccgccgggtc catgtggcac tcgcagtatc gcaccctatg
    11761 atgttgcctt ccgttaacag tacaacgtcg gcacccggta atttaatata catttcgtgg
    11821 ctcaatggct ggcctgtcaa cacccctgtc aacgcttcgc cccatacctc gcggtatgca
    11881 acgcatgact cggggacctt gtggattgct ggtccttcaa tggtcgggga ctttcacccc
    11941 ttgatctcta accggtctcc cggcgcacac
//
