LOCUS       NZ_CP084244             8801 bp    DNA     circular CON 24-MAY-2024
DEFINITION  Klebsiella pneumoniae strain T877 plasmid unnamed4, complete
            sequence.
ACCESSION   NZ_CP084244
VERSION     NZ_CP084244.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21849140
            Assembly: GCF_022577075.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8801)
  AUTHORS   Liu,C., Dong,N., Zeng,Y., Lu,J., Chen,J., Wang,Y., Chen,G., Shen,Z.
            and Zhang,R.
  TITLE     Co-transfer of last-line antibiotic resistance and virulence operons
            by an IncFIBk-FII-X3-ColKP3 hybrid plasmid in Klebsiella pneumoniae
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8801)
  AUTHORS   Liu,C.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-SEP-2021) Department of Clinical Laboratory, Second
            Affiliated Hospital, Zhejiang University School of Medicine, 88
            Jiefang Rd, Hangzhou 310009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v 0.4.4
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 200.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Name                   :: GCF_022577075.1-RS_2024_05_24
            Annotation Date                   :: 05/24/2024 04:24:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.7
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,588
            CDSs (total)                      :: 5,470
            Genes (coding)                    :: 5,329
            CDSs (with protein)               :: 5,329
            Genes (RNA)                       :: 118
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 141
            CDSs (without protein)            :: 141
            Pseudo Genes (ambiguous residues) :: 0 of 141
            Pseudo Genes (frameshifted)       :: 66 of 141
            Pseudo Genes (incomplete)         :: 81 of 141
            Pseudo Genes (internal stop)      :: 32 of 141
            Pseudo Genes (multiple problems)  :: 27 of 141
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084244.1.
            Bacteria and source DNA available from Department of Clinical
            Laboratory, Second Affiliated Hospital of Zhejiang University.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8801
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="T877"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="unnamed4"
                     /geo_loc_name="China: Guangdong province"
                     /collection_date="2020-09-03"
     gene            join(8788..8801,1..136)
                     /locus_tag="LDH71_RS27835"
                     /old_locus_tag="LDH71_27875"
     CDS             join(8788..8801,1..136)
                     /locus_tag="LDH71_RS27835"
                     /old_locus_tag="LDH71_27875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891188.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_process="GO:0019835 - cytolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="WP_032440454.1"
                     /translation="MKKVKTIFLFILIVAGFLLAACQANYIRDVQGGTVAPSSSSELTG
                     IAVQ"
                     /name="colicin release lysis protein"
     gene            1619..3580
                     /locus_tag="LDH71_RS27840"
                     /old_locus_tag="LDH71_27880"
     CDS             1619..3580
                     /locus_tag="LDH71_RS27840"
                     /old_locus_tag="LDH71_27880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_032448356.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYSLAGFFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIAWCAGGLV
                     AGVLLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMATNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPADFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARLAFSSPLRVDRREIPLKTFPVLAKTDPLAEY
                     QSEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            3580..4311
                     /gene="mobC"
                     /locus_tag="LDH71_RS27845"
                     /old_locus_tag="LDH71_27885"
     CDS             3580..4311
                     /gene="mobC"
                     /locus_tag="LDH71_RS27845"
                     /old_locus_tag="LDH71_27885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_094313769.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVAQRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDIIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGATGWRYLHRIALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRRANGIDEIWYICPDRATQVRVQRAILAVDEIVNPQTGEA
                     RKTVELDRERLFACFKFMMMD"
                     /name="mobC"
     gene            4340..4603
                     /locus_tag="LDH71_RS27850"
                     /old_locus_tag="LDH71_27890"
     CDS             4340..4603
                     /locus_tag="LDH71_RS27850"
                     /old_locus_tag="LDH71_27890"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_094313768.1"
                     /translation="MTNRVVAVGREALLMVAVMAGTLVVLAGNLLKLLWRVLPVLLLLY
                     GVVQFLARNPEIRDWLFLHFGQGGTAAILVGGILLCMRRLMK"
                     /name="hypothetical protein"
     gene            complement(4634..4813)
                     /locus_tag="LDH71_RS27855"
                     /old_locus_tag="LDH71_27895"
     CDS             complement(4634..4813)
                     /locus_tag="LDH71_RS27855"
                     /old_locus_tag="LDH71_27895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_094313767.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     EKLWLAMRDHLGEE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            4882..5259
                     /locus_tag="LDH71_RS27860"
                     /old_locus_tag="LDH71_27900"
     CDS             4882..5259
                     /locus_tag="LDH71_RS27860"
                     /old_locus_tag="LDH71_27900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="WP_094313766.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVMDTSE
                     LIRVYGELRQGETHERDGLRLPDETEKTAFDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTPRRWWQWGKR"
                     /name="entry exclusion protein 1"
     gene            complement(5321..5620)
                     /locus_tag="LDH71_RS27865"
                     /old_locus_tag="LDH71_27905"
     CDS             complement(5321..5620)
                     /locus_tag="LDH71_RS27865"
                     /old_locus_tag="LDH71_27905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_094313765.1"
                     /translation="MPYEVEWKQGAIEDVTTLFDYIAENSSLWDAQNVTDRVLTAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(5610..5873)
                     /locus_tag="LDH71_RS27870"
                     /old_locus_tag="LDH71_27910"
     CDS             complement(5610..5873)
                     /locus_tag="LDH71_RS27870"
                     /old_locus_tag="LDH71_27910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="WP_094313764.1"
                     /translation="MSNTTHYENANFLRELAENLPRILPESGPDKAALLQCLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="plasmid stabilization protein"
     gene            complement(6179..6424)
                     /locus_tag="LDH71_RS27875"
                     /old_locus_tag="LDH71_27915"
     CDS             complement(6179..6424)
                     /locus_tag="LDH71_RS27875"
                     /old_locus_tag="LDH71_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_032440458.1"
                     /translation="MTHLKLTPAEEKRLADAFVAQALAMGLKGNKTLPKAVKQQVRERV
                     LRAIQDEREARRAQEMRREKYEATETTFTWQPQRRR"
                     /name="hypothetical protein"
     gene            6751..8436
                     /locus_tag="LDH71_RS27880"
                     /old_locus_tag="LDH71_27920"
     CDS             6751..8436
                     /locus_tag="LDH71_RS27880"
                     /old_locus_tag="LDH71_27920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like bacteriocin tRNase domain-containing
                     protein"
                     /protein_id="WP_214301846.1"
                     /translation="MSGGDGRGPGNSGLGHNGGQASGNVNGTSGKGGPSSGGGTDPNSG
                     PGWGTTHTPNGDIHNYNPGEFGNGGSKPGGNGGNSGNHSGSSGGGQSSATAMAFGLPAL
                     ATPGAEGLALSVSGDALSAAVADVLAALKGPFKFGLWGIAIYGVLPSEIAKDDPKMMSK
                     IMTSLPADAVTETPASTLPLDQATVRVRQRVVDVVKDERQHIAVVAGRPMSVPVVDAKP
                     TKRPGVFSVSIPGLPSLQVSVPKGVPTAKAPPKGIVAEKGDSRPAGFTAGGNSREAVIR
                     FPKETGQKPVYVSVTDVLTPAQVKQRQEEEKRRQQAWDAAHPEEGLKRDYDKAKAELDA
                     EDKNIATLNSRIASTEKALPGARTAVQEADKKVKEAEANKDDFVTYNPPHEYGSGWQDQ
                     VRYLDKDIQNQNEKLKAAQASLNAMNESLSRDKAALTGAMESRKQKEKKAKDAENKLNE
                     EKNKPRKGTKDYGHDYHPVPKTEDIKGLGELKRGDPKTPKQGGGGKRARWYGDKKRKIY
                     EWDSQHGELEGYRASDGEHLGAFDPKTGKQVKGPDPKRNIKKYL"
                     /name="colicin-like bacteriocin tRNase domain-containing
                     protein"
     gene            8446..8703
                     /locus_tag="LDH71_RS27885"
                     /old_locus_tag="LDH71_27925"
     CDS             8446..8703
                     /locus_tag="LDH71_RS27885"
                     /old_locus_tag="LDH71_27925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891189.1"
                     /GO_function="GO:0015643 - toxic substance binding
                     [Evidence IEA]"
                     /GO_process="GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E3-like toxin immunity protein"
                     /protein_id="WP_032440455.1"
                     /translation="MGLKLRLTWFDKKTEEFKGEEYSKDFGDDGSVIESLGMPLKDNIN
                     NGEFDLKKEWVPLLQPFFKNQIKPDVDNCYIAFDYRDGNW"
                     /name="colicin E3-like toxin immunity protein"
     BGC             1751..8801
                     /name="RiPP-like"
                     /gene="RiPP-like"
                     /tool="antismash"
     Typing          complement(240..818)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.282"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          3581..4308
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="93.004"
                     /coverage="100.0"
                     /evalue="1.97e-140"
ORIGIN
        1 gactattttt ctttttatat tgattgttgc tggctttctt ctggccgcat gtcaggcaaa
       61 ttatatccgg gatgttcagg gcggaacggt ggcaccatcg tcctcctctg aactgacggg
      121 gatcgcggtt cagtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
      181 atcttttgcc ctgtaaacga aaaaaccacc tggggaggtg gtttgatcga aggttaagtc
      241 agttggggaa ctgcttaacc gtggtaactg gctttcgcag agcacagcaa ccaaatctgt
      301 ccttccagtg tagccggact ttggcgcaca cttcaagagc aaccgcgtgt ttagctaaac
      361 aaatcctctg cgaactccca gttaccaatg gctgctgcca gtggcgtttt gcgtgctttc
      421 ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc tgaacgggga
      481 gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga taccagtgtg
      541 tgagctatga gaaagcgcca cacttcccgc aagggagaac ggcggacagg tatccggtaa
      601 acggcagggt cggaacagga gagcgcaaga gggagcgacc taccggaaac ggtggggatc
      661 tttaagtcct gtcgggtttc gcccgtactg tcagattcat ggttgagcct cacggctccc
      721 acagatgcac cgaaaaagcg tctgtttatg tgaactctgg caggagggcg gagcctatgg
      781 aaaaacgcca ccggcgcggc cctgccgttt tgcctcacat gttagtcccc tgcttatcca
      841 cggaatctgt gggtaacttt gtatgtgtcc gcagcgcccg ccgcagtctc acgcccggag
      901 cgtagcgacc gagtgagcga ggaggcgcaa tttccccggc acctgcaaga cggtcaccgg
      961 acgcgctgat gtgggatatt gtcccctgat taagtctttc tattccctga cgatcaatta
     1021 tcaggatagg tgaaccatgt cggtttttcg ctgaacattt tacgttaacc tatctctgtt
     1081 gcttttttct ccttgcggag aaatgcaagt catccccgct ggggcgtggc tgacttcagg
     1141 ggaaaagacc ttgcttacgc tggctggacg ccaccgacgc acggtctttt cccctctttc
     1201 tggctctgtc cggctggacg ccggccacgc cagaaattca cccggccccc gtcaggctca
     1261 cgcctgacca cccacaaaat ccacggtgct ggacgcacct gacgaagcag caaagctgct
     1321 cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg ccggaacacc
     1381 ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt gctcgttgtt
     1441 cctccggggc tgcgcctttt ccgcatgggg cggaaagagg ctggcctccg cggtgaaagc
     1501 tgaacgcaat caccacggac gtgaccgccg ccgccatgcc ttggggcatg tccgcggctt
     1561 gtgcagcagg ggctgcacaa cacgccggta aacccgtcgt ctgaaggaga gagattgcat
     1621 gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc ggcagattta
     1681 cccgtgggtc agctggctgt accgggagta ttacagtctg gccggttttt ttctggttat
     1741 cggcctgttt ttcggtctgg cagaactctg ggccgggcgg atgcgattcg tggggctgag
     1801 tcataaggag cggcgggagt atgccgatcg ctataagcgc gataataacc ggactgcgcc
     1861 attttcggtg ctggcctcgc tggattcgcc cgggcggatg gtcagggcac ggcgacatgt
     1921 ctggaactac gcatactggc tgaccggcgc tgccggtctg gcccttctgg gggggctggt
     1981 tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc tctacgcgaa
     2041 cgcagattat tacctgctgc agccggctaa cctgttcggg attgcctggt gtgccggagg
     2101 tcttgtcgca ggcgtcctcc tggcgcggct gctggcgttc ctgctgcttg acgggcattt
     2161 tgcggccgct gacgaggcgg tgaacgcgaa actgaatcag gagagccggc gcagcagcca
     2221 gcggaccgga gaaatgaccg acgtccgcca cctgcatttc ggggagccgg taccggttaa
     2281 cgcgctggcc gatttttcca cggagcaggc ccggaaacag caggcggtgt ttctgggtaa
     2341 ggatgagcag ggccagccgg tgctggtccc gcgtgatacc tggcgcaaga ccaacattca
     2401 gatcctcggc ctgcccggga gcggcaagag cgtgatggcc accaacgcgc tcattcgctg
     2461 cgtacgcgat ttcggggacg cggtggtgta cttcgacccg aaaggggacg cgtgggcgcc
     2521 gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc cggggaaacc
     2581 ggcgcagctg aatctgttcc gcgatctcga ccagtacgcc ctgaagaatc ttctggttgc
     2641 cggatttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca gcgagcagaa
     2701 agcggcgaag ctgattgcgg agcagtttcc gcagggggca aatattcagc aggtgctggc
     2761 ggcggcgtac gcactgccgg aggccctgaa aaaggacgtg aaggggctga tcaccaagct
     2821 ggagaacgtg gccgacctga gcgtccttca gacggacagc ggcatcgacg tggcggggat
     2881 aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg cggtgatccg
     2941 ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg acgagttccg
     3001 gaggtggccg cacgcgagca tcatgctcga tgagattaaa tatctgctgt cgaagtacgt
     3061 gctgaacgcg ctggggacgc tgcgcagccg ggactgcaac ctgctgctcg cgcaccagtc
     3121 gctgggcgac ttcggccagt gcgggcagga tctgcccgct gattttgtga aaaccacggt
     3181 actggataac acgccgatcc gctggttcta ccgggcggcc agccaggagt ccgctcagtg
     3241 ggcggccgga cagaccgggg agatccgggt ggacgtggag cgccgccggg ccagccggga
     3301 ggcggggaac gtggagcata tcagcgggga cagcttcatc cagaaggagg cgcggccgct
     3361 gtttgacgtg aacaccctgc aacatctgcc tgacgggttc gcggtgatga ccgggctggg
     3421 cgtggcgcgg ctggcgttca gcagcccgct gcgtgttgat cgccgggaaa ttccgctgaa
     3481 aaccttccct gtgctggcga aaacggaccc gctggcggag taccagtcgg aggccgggcg
     3541 gcagcggccg ggtgatgatg attttgcggg gctgtactga tggcactgga gcgatacaac
     3601 gtcagccacg cgaagcggca ggcacggaac gcggagaaaa cacggctgac cctgcgctgg
     3661 ctgcgggaag aactgtgttc cacggcggag ctggttgcgc aacggctggg gattgcggcg
     3721 gtacagccgg tttaccgctt cctggacagc ctggtggcga aagggctgct ggtccgggcg
     3781 aaatacccgg ttgacggccg tcaggtcagc gtgtgggggc tgacgccgca cggggtggcg
     3841 ttctcgttcg acgaggacga gccgctgacg gacattattc cgtttcagcc gtcgcgggtg
     3901 tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgcctcgc catggaggcc
     3961 cggggggcca ccggctggcg gtacctgcac cgtattgccc tgaaagggat gaaggtgccg
     4021 gacgcgctgg cggagctgga cggcagaacg gtggcgtttg aggtggaacg caccgtgaag
     4081 agccgcaggc gctatcagga ggtggtgtcc gggtatctgt ttaaccgccg ggcgaacggc
     4141 atcgatgaaa tctggtacat ttgcccggac cgggcgacgc aggtcagggt gcaacgggct
     4201 attctggcgg tggatgaaat cgtgaacccg cagaccggcg aggcccgcaa aacggtggag
     4261 ctggaccggg agcggctgtt tgcgtgcttt aaattcatga tgatggactg agcttataca
     4321 ttcactcaga gaggttttta tgacaaatcg tgttgttgcc gtggggcgtg aagccctgtt
     4381 gatggtcgct gtcatggccg ggacgctggt cgttctggcc ggaaacctgc tgaaactgct
     4441 ttggcgcgta ctgccggtgc ttctgctgct ctacggggtt gtgcagtttc tggcgaggaa
     4501 cccggagatc cgtgactggc tgtttctgca tttcgggcag ggaggcacgg cggccattct
     4561 tgtcggggga atactcctct gtatgcgccg cctgatgaaa taatgccgga cagcaccggc
     4621 tcggccggtg ctgttattcc tccccgagat ggtcccgcat tgccagccag agcttttctg
     4681 catcctcatg cagccgttcg cacatgtcgg ccggggtatc catatccagc tcgttcagtt
     4741 tctcgagcaa cagcagcgac tggtccttaa tgaatttcgc catgttgagg gcggtttttt
     4801 cctgcttcgt catgtttctg tccttccggg cacggtctgc gtctgtcggg tatactgtct
     4861 caaaaatagc ggggtgagac aatggcgtgg gtgacggtga gacaggcgac ggaactgacg
     4921 gggaaagccc ggagttccct gtatcgggat atggcgaaag gccgcgtatc ataccgtacg
     4981 gaagctgatg gtggtcgggt gatggatacc agcgagctta tccgggtgta cggtgagtta
     5041 agacagggtg agactcacga gcgggacggg ttgagactgc cggatgagac tgaaaaaacg
     5101 gccttcgacg cgttgatcgc ggagataaaa gcactcaggg aggaggtggc cggactgcgg
     5161 caggaaatgc aggccatgag actgctggaa cacaaaccgg agacagcaga gacaccggcc
     5221 ggaacaccgc gccggtggtg gcagtgggga aaacgctgac gcgctgtgct tgtccaaccg
     5281 gcgacggcca cgcaatgaat tgcgcccccg tcaccggtta tcagcgaacg gtatggggat
     5341 tctggcgctg gccaaccacg gccagaaccc ggactttcct ggtctggtca tcaacatcat
     5401 acaggacatg ctgtcccatc agactgatcc ggcgaacgtc ctcgccatag cgtgagtcca
     5461 cttcatagag gcgcgggaag tcggccagtt tgtccgccgc agtgagaacg cgatcggtaa
     5521 cgttctgcgc atcccacagg gatgaatttt ccgcgatata gtcaaacagc gtcgttacat
     5581 cctcgatggc tccctgtttc cattccacct catacggcat cgcgcagttc ctgataacgg
     5641 ccatgcaggc gctggcggag ttgttccgtg gtcatgcccg gacgcgtgtc agcacgggcg
     5701 gcggttactt tggcgcgaac ctgatcttca tactctgcct gggccagttc ttcgttagcc
     5761 aagcattgca acaacgcagc tttgtccggg ccactttccg gcagtatgcg gggcaggttt
     5821 tcagccagtt cccgcaggaa gttggcgttt tcgtagtgtg tagtgttgct cattgcggag
     5881 actcccggat taatggggtg atatgaagaa gtttagcagg gggcggtctg aaagaatatg
     5941 ctcgtgtcag tcaggctgat gaagacgaaa gctggtaaaa aagggcttac ggcacccatt
     6001 ttacgtcaaa ctgcacgcca tgcagttgcg tgcataggca tgcattagga taaagtttac
     6061 cgggcgcgtt tccggcggtt ttccgggtgg tttgttgcct gttttatccc gtagccgccg
     6121 gaaatgccct cagaccgtct gagcggtgcg cgtaatgcga cgttatggta aatacccctt
     6181 accggcggcg ctgtggctgc caggtgaagg tggtttccgt agcctcgtat ttctcccgac
     6241 gcatttcctg cgcccgcctg gcttcacgtt cgtcctgaat ggcacgaagc accctttccc
     6301 gcacctgttg ttttaccgcc tttggcagcg ttttgttccc tttcaggccc atggccagtg
     6361 cctgagcgac aaaggcgtcc gccaggcgct tttcttcagc gggagtcagt ttcaggtgcg
     6421 tcatagtggc ctccgggcgt ctcaggtttc ccgaacagtt taaccgcagg tcctctcctc
     6481 atgccagtta gctttcagat gtgtctatac catctgaaat gtaagtgtga tgaatgtctt
     6541 aaataataaa atttgttgcc atgagaaatt ttcgggaaaa gacgcgatct gcttacaggt
     6601 ggctccgggc gcttttttgt gcctgaagat gagaactaat ctcatatatt gacacctgaa
     6661 aacgggagga gtaaggtaat aattatactg tgtatatgta cagtatgttg gctttaattt
     6721 attgttttaa atgtcaaaga ggaaaacgat atgagtggtg gagacggtcg aggtccgggt
     6781 aattcaggtc tgggacataa tggtggtcag gccagtggga atgtgaacgg tacgtctggt
     6841 aaaggtggcc cttcatcagg tggtggtacg gatccaaaca gcgggccggg ctggggtacg
     6901 acgcatacgc cgaatggaga tattcataac tataatccgg gggagtttgg taacggcggg
     6961 agtaaacccg gtggtaatgg tggtaacagc ggcaatcata gcggtagctc tggtggcgga
     7021 cagtcttcgg ccactgcgat ggccttcggt ctgcctgccc tggctactcc gggggctgaa
     7081 ggactggctt tatcagtttc cggcgatgcg ttgtcggccg ccgttgctga tgtgctggct
     7141 gccctgaagg ggccgtttaa gtttggtctg tgggggattg cgatctacgg tgtgctgcct
     7201 tctgagattg caaaagatga tccgaaaatg atgtcaaaaa ttatgacgtc attaccggcc
     7261 gatgcggtga cggagactcc ggcaagtact ttaccactgg accaggcgac ggttcgtgtc
     7321 agacaacggg ttgtggatgt ggtgaaggat gagcggcagc atattgcggt tgtcgcaggt
     7381 cggccaatga gtgtccctgt ggtggatgcg aaaccgacaa aacgtccggg ggtattcagt
     7441 gtgtcgattc cgggtctccc gtctctgcag gtgagcgtac cgaaaggtgt tccgacagcg
     7501 aaagccccgc caaaaggcat tgttgctgaa aaaggtgatt cacgtccggc tggttttaca
     7561 gccggtggta actcccgtga ggccgttatt cgtttcccga aagagaccgg acagaagccg
     7621 gtttatgtgt cggtgacaga tgttcttacc ccggcacagg taaaacagcg tcaggaggaa
     7681 gaaaagcgtc gccagcaggc atgggacgcc gctcatccgg aagaggggct gaaaagagac
     7741 tatgataaag cgaaagctga gctggatgcc gaagataaaa atattgcgac cttaaacagc
     7801 cgcattgcat cgacagagaa ggcgctcccc ggtgcaagga ctgctgttca ggaagccgat
     7861 aaaaaggtaa aagaggcaga ggcaaataaa gatgattttg tgacttataa cccgcctcat
     7921 gaatatggct ccgggtggca ggatcaggtt cgctatcttg ataaggatat tcagaatcag
     7981 aatgagaaat taaaagcggc tcaggcatct ttaaacgcaa tgaatgaatc cttatccaga
     8041 gataaggctg cacttaccgg ggcgatggag agccggaaac aaaaggagaa aaaagcgaag
     8101 gatgcagaaa ataagttaaa tgaggaaaag aacaagcctc gcaaggggac taaagactac
     8161 ggccatgatt atcatccagt acctaaaaca gaagacataa agggacttgg agagttaaag
     8221 cgaggtgatc ctaaaacacc taaacaaggt ggtggtggta agcgggcccg atggtatggc
     8281 gataaaaaac gtaaaattta tgaatgggat tcccagcacg gtgagcttga agggtatcgt
     8341 gccagtgatg gcgaacacct aggcgcattt gatcccaaaa cgggtaagca ggttaaaggg
     8401 ccagatccaa aacggaacat taaaaaatat ctttgagagg taaatatggg gcttaaatta
     8461 cgtttgacat ggtttgataa gaaaactgaa gagtttaaag gggaagagta ttctaaagac
     8521 tttggtgacg atggttctgt cattgaaagc ttgggaatgc ctttaaagga taatattaat
     8581 aatggtgagt ttgatttgaa gaaagaatgg gttcctttac tacagccatt ttttaaaaat
     8641 caaataaaac ctgatgtgga taattgttat atagcttttg attatcggga cggtaactgg
     8701 taacaaaaaa ttatcaagga tgatttgaaa tactgacttc cggtgttcag ggatgaatgc
     8761 cggagtcttt aaatacaggg aaagtatatg aaaaaggtaa a
//
