LOCUS       NZ_CP084184            11625 bp    DNA     circular CON 22-AUG-2023
DEFINITION  Enterococcus faecium strain 4995/20 plasmid p4995_4, complete
            sequence.
ACCESSION   NZ_CP084184
VERSION     NZ_CP084184.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21850488
            Assembly: GCF_020221735.2
KEYWORDS    RefSeq.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11625)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Vancomycin-, tigecycline- and linezolid-resistant Enteroccoccus
            faecium carrying both vanA and vanB genes
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11625)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (28-OCT-2022) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
  REMARK    Nucleotide sequence updated by submitter
REFERENCE   3  (bases 1 to 11625)
  AUTHORS   Wardal,E., Sadowy,E., Zabicka,D. and Hryniewicz,W.
  TITLE     Direct Submission
  JOURNAL   Submitted (27-SEP-2021) Molecular Microbiology, National Medicines
            Institute, Chelmska 30/34, Warsaw 00-725, Poland
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 24-FEB-2021
            Assembly Method                   :: Unicycler v. 0.4.7
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 670.0x
            Sequencing Technology :: Oxford Nanopore MinION; Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 08/21/2023 08:47:26
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,050
            CDSs (total)                      :: 2,960
            Genes (coding)                    :: 2,831
            CDSs (with protein)               :: 2,831
            Genes (RNA)                       :: 90
            rRNAs                             :: 6, 6, 6 (5S, 16S, 23S)
            complete rRNAs                    :: 6, 6, 6 (5S, 16S, 23S)
            tRNAs                             :: 68
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 129
            CDSs (without protein)            :: 129
            Pseudo Genes (ambiguous residues) :: 0 of 129
            Pseudo Genes (frameshifted)       :: 38 of 129
            Pseudo Genes (incomplete)         :: 86 of 129
            Pseudo Genes (internal stop)      :: 34 of 129
            Pseudo Genes (multiple problems)  :: 25 of 129
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084184.1.
            Bacteria and source DNA available from Molecular Microbiology,
            National Medicines Institute, Chelmska 30/34, Warsaw 00-725,
            Poland.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..11625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /strain="4995/20"
                     /isolation_source="hematoma"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /plasmid="p4995_4"
                     /geo_loc_name="Poland: Bydgoszcz"
                     /lat_lon="53.1234 N 18.0084 E"
                     /collection_date="2020-09-25"
                     /collected_by="National Medicines Institute"
     gene            1..933
                     /locus_tag="LDJ74_RS15620"
                     /old_locus_tag="LDJ74_15620"
     CDS             1..933
                     /locus_tag="LDJ74_RS15620"
                     /old_locus_tag="LDJ74_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /GO_function="GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_002313300.1"
                     /translation="MANEIVKYEPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIRF
                     YFSQLKDLSAYKQTAGEVFIKDLARTYDHLMDLRFGRKSNGGLTIERFTMFTDFEIHGE
                     VDNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWAEFS
                     KEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFTW
                     KAEINHADDFSKGKQEDLRIKLFNIEHNGELTQKEKWRAKDRILNLPLGTHEADFKKQQ
                     QTEKEEAEKQSISNELKQDLLENLQNLFD"
                     /name="replication initiation protein"
     gene            950..1420
                     /locus_tag="LDJ74_RS15625"
                     /old_locus_tag="LDJ74_15625"
     CDS             950..1420
                     /locus_tag="LDJ74_RS15625"
                     /old_locus_tag="LDJ74_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="WP_002302544.1"
                     /translation="MIDKTIKELAEEFHVSKQAIRKRLSADFRENYVQTVTRNGVKTLV
                     VSYKGYLLLKQHFLGSNTTGNQVETVASNDENSIIVFLHEQLQEKDTQIKQMQKLLDQQ
                     QQLTLQANQQIEKLQEQLQLAYTEEPEEEELTSVNKENNNAITTEKKWWKFW"
                     /name="DUF536 domain-containing protein"
     gene            1504..1956
                     /locus_tag="LDJ74_RS15630"
                     /old_locus_tag="LDJ74_15630"
     CDS             1504..1956
                     /locus_tag="LDJ74_RS15630"
                     /old_locus_tag="LDJ74_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339186.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_060806732.1"
                     /translation="MTISKVIKQKRIEKQLTQEDLAEMLLVSKKTISNWENGRTIPDTE
                     NLIQFAKLFDLSLDNILLGEEKIMVKNHTKTLILLFHFVIFLSLVFTLIQSGFFYFSHS
                     IATWLLIFFLSLLLIINTLIIIFNKHRNLFLHSIFPIVLLIFLYFI"
                     /name="helix-turn-helix transcriptional regulator"
     gene            2083..3264
                     /locus_tag="LDJ74_RS15635"
                     /old_locus_tag="LDJ74_15635"
     CDS             2083..3264
                     /locus_tag="LDJ74_RS15635"
                     /old_locus_tag="LDJ74_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313302.1"
                     /GO_process="GO:0051301 - cell division [Evidence IEA];
                     GO:0009252 - peptidoglycan biosynthetic process [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="WP_002302539.1"
                     /translation="MKNNRIYKNQLNYGLLLPVFLLCIIGLLSLYVALYHDSYTNSKIT
                     KELIKQVLWYSIGFTSAITIYFFNPKILWKLTPIFYGLCIIMLLLLLKFYNPQLAVLTG
                     AKNWFYIAGFSFQPTELVKIGYTLMLAKITVNYQQKSITSNTKDDWIYLLKTFIATLPI
                     GILLFFQNDLGTMLVFIVIYIFILFTSNINIKIIAPIIAIGVVLSLIFLYLVISDTGRD
                     FLLKIGFHDYQFKRIDSWLNPFLDPNGSSYQLANSLIAIGSGGLLGTGFNVSNIHVPVR
                     ESDMIFSVIGENFGFIGSCLVIFLYFYLIYQMYKTCFKSNNLFFTYIGIGIVAMIFFHV
                     FENIGASIGLLPLTGIPLPYISQGGSAILSNFLGLGFMLCCSRYTNEKKGTQK"
                     /name="FtsW/RodA/SpoVE family cell cycle protein"
     gene            complement(3480..4628)
                     /gene="tnpB"
                     /locus_tag="LDJ74_RS15640"
                     /old_locus_tag="LDJ74_15640"
     CDS             complement(3480..4628)
                     /gene="tnpB"
                     /locus_tag="LDJ74_RS15640"
                     /old_locus_tag="LDJ74_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element RNA-guided
                     endonuclease TnpB"
                     /protein_id="WP_002287525.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYEE
                     VKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRFKS
                     KKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKY
                     YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLS
                     RRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKD
                     GKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
                     /name="tnpB"
     gene            complement(4645..5049)
                     /gene="tnpA"
                     /locus_tag="LDJ74_RS15645"
                     /old_locus_tag="LDJ74_15645"
     CDS             complement(4645..5049)
                     /gene="tnpA"
                     /locus_tag="LDJ74_RS15645"
                     /old_locus_tag="LDJ74_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="WP_002287522.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERISE
                     SYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKEMF
                     WSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
                     /name="tnpA"
     gene            <5198..5314
                     /locus_tag="LDJ74_RS15915"
                     /pseudo=""
     CDS             <5198..5314
                     /locus_tag="LDJ74_RS15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313303.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /name="bacteriocin"
     gene            6120..6293
                     /locus_tag="LDJ74_RS15650"
                     /old_locus_tag="LDJ74_15650"
     CDS             6120..6293
                     /locus_tag="LDJ74_RS15650"
                     /old_locus_tag="LDJ74_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_162897362.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYNS
                     PNEELFVKQTDK"
                     /name="hypothetical protein"
     gene            6751..7851
                     /locus_tag="LDJ74_RS15655"
                     /old_locus_tag="LDJ74_15655"
     CDS             6751..7851
                     /locus_tag="LDJ74_RS15655"
                     /old_locus_tag="LDJ74_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729425.1"
                     /GO_function="GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /GO_process="GO:0006508 - proteolysis [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C47 family peptidase"
                     /protein_id="WP_211182505.1"
                     /translation="MIGIFSIGITNTYASENSNFNINSQPIEEQAINFAKTDFEHSITS
                     ILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFSDADNTIKYIYTVKKNTNGEY
                     SGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNGQIKLLWKSPLSTEKNGVSSFT
                     EKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSGDNFIMINWRIAETQTSFPWCAAYV
                     EAAILCNKTDVHATSARAIMHYTYPNLNNSQIESKSLNQDQIIKYANYCGVYPKKVARI
                     LNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALALFGWVNQGGYQTYYVWNPWYNYPTV
                     ISANAGPVTIPVPGGGFTWDNTITNW"
                     /name="C47 family peptidase"
     gene            7824..8267
                     /locus_tag="LDJ74_RS15660"
                     /old_locus_tag="LDJ74_15660"
     CDS             7824..8267
                     /locus_tag="LDJ74_RS15660"
                     /old_locus_tag="LDJ74_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002318191.1"
                     /translation="MGQYHYKLVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGT
                     WQTKDHSERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLT
                     LRSNKKQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
                     /name="hypothetical protein"
     gene            8569..8949
                     /locus_tag="LDJ74_RS15665"
                     /old_locus_tag="LDJ74_15665"
     CDS             8569..8949
                     /locus_tag="LDJ74_RS15665"
                     /old_locus_tag="LDJ74_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="WP_047936909.1"
                     /translation="MSEQNQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEALQ
                     MSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPGDA
                     QRLSENLKIMQKELQKIWQQLK"
                     /name="MobC family plasmid mobilization relaxosome protein"
     gene            8931..9845
                     /locus_tag="LDJ74_RS15670"
                     /old_locus_tag="LDJ74_15670"
     CDS             8931..9845
                     /locus_tag="LDJ74_RS15670"
                     /old_locus_tag="LDJ74_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="WP_002289347.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIRM
                     LYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHNHI
                     VINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKLKEK
                     NEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKKKVRS
                     NRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTPEQPEI
                     RPARRNHEQHVERGTDHQQELSL"
                     /name="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            9805..10083
                     /locus_tag="LDJ74_RS15920"
     CDS             9805..10083
                     /locus_tag="LDJ74_RS15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_002348700.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDLQ
                     VKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
                     /name="hypothetical protein"
     gene            10085..10774
                     /locus_tag="LDJ74_RS15675"
                     /old_locus_tag="LDJ74_15675"
     CDS             10085..10774
                     /locus_tag="LDJ74_RS15675"
                     /old_locus_tag="LDJ74_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_060806731.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFLG
                     CIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQVLAQKIEKFTACY
                     EDMNDIFVKQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKVEEA
                     CSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQAINIQNLTKKETALNYLKSVKEW
                     LDQQQQN"
                     /name="hypothetical protein"
     Typing          2..933
                     /name="rep_cluster_1249"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          8932..9842
                     /name="MOBP"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 atggcaaatg aaatcgtaaa gtatgaacca gaattaaata ccattccttt aagaaaattt
       61 accccaatag aaatgaactt gtttttttct attgtctcta gaatgcgcga taaaggtaat
      121 caaaaaatac gcttttattt cagccaactt aaagatttaa gtgcgtataa gcagactgca
      181 ggagaagtct ttataaaaga tttggcaaga acatacgatc atttgatgga tttacgtttc
      241 ggaagaaaaa gcaatggtgg attaacaata gaacgattta caatgtttac tgattttgaa
      301 attcatggag aagtcgataa cccttatgta gatataaaag tccacgaaaa agctttgcct
      361 ttacttaata atctagaaag ctgggtacgt ttctctttaa cagaatttcg agatttaaat
      421 agcagttatg ctaaaacaat gtttagattg ctaaaacaat accgaactca aggatgggct
      481 gaattttcta aggaagattt tttagagcta ttagatattc ctaaaagcta tagacaaaca
      541 aatataaatc agtttgtact caaaccaata aaagaagaac taactccact atttaaagga
      601 ttaactattc gtaaaaaata tggtaaaggt cgaggtaaac ctgttattgg ttatcgtttt
      661 acttggaaag ctgaaatcaa ccatgctgac gatttttcaa aagggaagca agaagattta
      721 cgtataaaat tatttaatat tgaacacaat ggagaattaa cacaaaaaga aaaatggcgt
      781 gctaaagatc gaatactaaa tcttccgtta ggtactcatg aagctgattt taaaaaacaa
      841 caacaaaccg aaaaagaaga agcggaaaaa caatcaatct caaatgagct taaacaagat
      901 ttattagaaa acttacaaaa cttattcgat tgaagcataa ggagtattta tgatagataa
      961 aacgatcaaa gaactcgcgg aagaatttca tgtttcaaaa caagcaatta ggaaaaggct
     1021 atcagctgat tttagagaaa actatgtgca aactgttact aggaacggag tgaaaacctt
     1081 agttgtcagc tacaaaggat atttgctttt aaaacaacat tttttaggta gtaacaccac
     1141 tggtaaccaa gtagaaaccg ttgctagtaa cgatgaaaac tcgattattg tttttttaca
     1201 tgaacagctc caagaaaaag atactcaaat aaaacaaatg caaaagttac tcgaccagca
     1261 acaacaactc accttgcagg caaatcaaca gatcgaaaaa ctgcaagaac agttacaatt
     1321 agcttatacg gaagaacctg aagaagagga attaacttct gtcaataaag aaaacaacaa
     1381 tgcaataaca acagaaaaaa agtggtggaa attttggtaa caattaccaa ctttccataa
     1441 agaagaaatc aatttctata gactctatct ttctaagaat aaaaaatatg tttaggtgat
     1501 caaatgacta taagtaaagt aatcaaacaa aaaagaatag aaaaacaact aactcaagaa
     1561 gatttagctg aaatgctttt agtatctaaa aaaaccattt ctaattggga aaatggtcga
     1621 acaataccag atacagaaaa tttaattcag tttgctaagt tatttgattt atcactagac
     1681 aatattttgt taggagaaga aaaaattatg gtaaaaaatc atacaaaaac actaatacta
     1741 ttatttcatt ttgtaatttt tttaagttta gttttcacct taattcagtc cggattcttt
     1801 tattttagcc attcaattgc cacttggtta ttaatatttt ttctaagttt actattaata
     1861 attaatactt taattattat ctttaataaa catagaaact tatttcttca cagcatattt
     1921 ccaattgttt tattaatttt tctatacttt atataaaaag atagaactat ttagtaagat
     1981 aaaaaagtag ctaattgtaa ttttcgtcta aaactcaaaa ctagtaaaaa taactttatt
     2041 gtgttatttt tatttataaa ttatgaaaaa ggacctttcg aaatgaaaaa taatagaatc
     2101 tacaaaaatc agttgaatta tgggctttta ttgccagttt ttctgctatg tatcattgga
     2161 ttactttctt tatatgttgc tttatatcac gactcttaca ctaattcaaa aatcacaaaa
     2221 gaattaatta aacaagtatt gtggtatagt attgggttca cttcagcaat aactatttat
     2281 ttttttaatc ctaaaatttt gtggaaatta actcctatat tttacggatt atgcatcata
     2341 atgttattac ttctcttgaa attctataat ccacaattag cagtcttaac tggagcaaaa
     2401 aattggtttt atattgctgg tttttcgttt caacctactg agttagtcaa aataggatac
     2461 acccttatgc tagctaaaat aaccgtcaat tatcagcaaa aatctattac tagtaatacg
     2521 aaagatgatt ggatttattt acttaaaact tttattgcta ctcttccaat aggaatatta
     2581 ttattttttc agaacgatct aggaacaatg ttagtcttca ttgttattta tatttttatt
     2641 ttgtttacgt ctaacataaa tataaaaata attgccccaa taatcgccat tggtgttgtt
     2701 ttatccttaa tatttttata tttggttata tcagatacag gaagagattt cttattaaaa
     2761 ataggatttc acgactatca gtttaaaaga atagactcat ggttgaatcc atttttagat
     2821 ccaaatggat ctagctatca gttagcaaat agtttaattg ctattggctc tggcggactt
     2881 ttaggtactg gttttaatgt atcaaacatt cacgttcctg ttagagaatc cgatatgata
     2941 ttttctgtaa ttggagaaaa ttttggcttt ataggaagtt gtcttgtaat attcctatac
     3001 ttttatttaa tttatcaaat gtataaaaca tgttttaaaa gcaataactt atttttcacc
     3061 tatatcggta taggcattgt tgcaatgatt ttttttcatg tttttgaaaa tataggtgca
     3121 agtatcggat tactacctct aactggtatt ccactcccct atattagtca aggtggttct
     3181 gccattctta gtaatttttt ggggttggga tttatgttgt gttgtagtag atatactaac
     3241 gaaaaaaaag gaacacaaaa atgaattcaa aataacactt tatgaataga atgtctattt
     3301 caataaaaat tatgataccg tgaactactc accacttagc taaacctgac ggttcttaac
     3361 gcttgaagtg ggagcttctt gggaatagag catacttgat agcgtatttc tttactaaca
     3421 gcggtgtctc ttatttacca agctatcccc gtagttccta cggttcttga tgattggatc
     3481 taagccaatc ccatagaatg tagtcttaga ccttcggtca gaatattgat actagcattg
     3541 atatcacgat catgatgagt atgacaaaca ggacaagtcc attctcgaat ttcaagagat
     3601 ttcttgccat ctttatgtcc gcactctgaa caaatctgac tagacggaaa ccatttatct
     3661 actttgatga tttttcgtcc ataccagtca gccttgtatt gtaatttagt cacaaacttt
     3721 gaccaagaaa catcagaaat acttttagct aatttatgat ttcgcaacat accttttgtg
     3781 tttaagtctt caatacagat aatatcgtga tttttgatca tttctgtact caacttattc
     3841 agaaaatcag tacgttgatt cattactttt tcatgcaatc tagctacttt gcgtttttgt
     3901 ttttgataat ttttagcttc aaatagattg gtacctttat ttttagccaa taaagcacgc
     3961 ctagacaatt tgcgttgttc acgctttagt ttcttttcca ttttggacgt gaatttatta
     4021 ttatcaattt tttgtccatc agaaagaatc gcaaagtcca taatacctaa gtcaatacca
     4081 attgtggaat tagttttagg taattcgata atttcttctt tacacaacaa agaaatatag
     4141 tatttaccgc tagaatgacg tgatattgta gcagatttga taatcccttt tggttgtcta
     4201 tgaagcttaa ttcttactaa tgacttcaat ttaggaactt tgatgaattt actatcaatc
     4261 aaagcaactg taccattttg attattcgtt gtataactct gaacaggatt tttcttactt
     4321 ttgaaacgtg gaaatccaac ggatttatcc cgaaaaaaat tcttgtatgc tttatctaaa
     4381 ttaagttggg cattggctaa agcaaggcta tcaatttctt tcaaaaacgg aaattctttc
     4441 ttgtatttag ctggtgtcgg aaaagtcatt tttttagaag aatcattttt aacttcttca
     4501 tacgcctttt ttcggtcatc aagcattaga ttgtagacct tacggacaca accaaaagat
     4561 ttggcaaaga agatttcttg ttcctctgtt ggatagattc tgaatttgta tgcttttagt
     4621 tgttccataa agttcaccta ctttctattt atgattgttt ccttgatttt ggatatactt
     4681 cttaatcaca tcaattggtg cgccaccagt tgtcagaaga caaaaacttt tagaccagaa
     4741 catttctttc caaagaaatt gtttgactct tggaaagtca cgttttatca atcttgagct
     4801 ggcactttta taggcattga tgaattttgt caattctgtt ttaggttgag ctttgaacat
     4861 aatatgaacg tggtctttat catgattcca ctccactaaa gtaatgtgat acgattctga
     4921 aattctttca aaagtagttt tggcataatc agatatttca tcatcaatca cttgtctacg
     4981 atatttcgtt actaaaacaa ggtggtaata aagaagaaat actgaatgat tatttgtatc
     5041 taattccatg ttatatcaac tcatttcttg tatagactga ttgtaacatg gaacaaaata
     5101 aaaaggcaat acgccttagt tgtcggtctt cgagttacca acggtaaccc tcataccgaa
     5161 cggcattcat cacccaccta tagaggatgg gcgacttctg cctaattctg ttaaaatgtt
     5221 agtaaatatt agaggtaaac aaactatgcg tacttgttac cttatgagtt ggaatgctag
     5281 tagtagaaca gccaaatatt attactacat ttagtaaaaa caaaacattc tttctataca
     5341 ataaaatagc gtatagagag gatgttttta atttgaaaag gagtggttat ctttatgaaa
     5401 aatagatcat ttgctatatt agtattatcc gtaattttat tagatatatt gttaataaca
     5461 ttagctttaa aaactttaaa tttagtaaca agttttgtca ttataccagt aattatagga
     5521 atgtctattt atttcatcaa aaaaaataaa caattttttt gaatgaaatt acgggcgaaa
     5581 ataatcactc aaaattatta ataaaaacga atcaactact ttttctgaag aaacatacaa
     5641 ccgtaaacaa caattgttgt ttacggttgt aaattatcaa actatatggt gacttgttgc
     5701 ttaaaatagg ttgaaatttc gtttattttt tttgagaaat tttacccacg tactacccat
     5761 gtacctatcc acgtattacc catgtacccc cccatgacaa atgggcggag gggcttgcgg
     5821 cgcttggtat taaaggcatt atccgcctcc caggtttctg accattttct ctcataagtg
     5881 gggtttttac cgcgttgaga gaaaatggtc agaaacctaa acgatcggca aagaaaacat
     5941 accaagcgcc acaagcaaaa aaagttagag taacttccag aaaagaaaaa tttttagaac
     6001 aacttttgaa aatttgattt tgatttatat ccgttcaatt taaacatttg ataaactgaa
     6061 ggctagtatt taaaatgtgt atacaagtgg tagcgttaaa aaaagtggag gtataaccta
     6121 tgttaaaaaa aactgtgttg gtactgggtt ctatttctct aggaatatta ggttttagcc
     6181 ccatgctatt atccgtaatt ggaaaaaaca attccaacag agaacttaaa ttaaattcaa
     6241 attattacaa cagtcctaac gaagaattgt ttgtaaaaca aacggataaa taataaaaaa
     6301 gactgctctt tcgagcagcc agttaaaaaa taatgagtgt ttattcttga aaaagcacgc
     6361 gcttatgaag cgctaccaaa aatttaccat attttacaaa aaataacaag tcttaaaaaa
     6421 atgacagata aaaatgcaca aagaattaga tatagataac cgcgaaaaat caagctttag
     6481 aaagttgctt ttagctagac caattaaaaa tcacaaagtg agcgtctcta tccattggta
     6541 gaggggctac ttggcatagt gtacattatg ttaactaacg ctataaataa cgattatgtt
     6601 cccaaaacaa tattttatga actataacaa aaatttgtta tcgcatattg tatattttaa
     6661 ttataggtgt ataccactta aatttaacac aaaggcgtga ttattttgaa acccaaaaag
     6721 aaaatttatc taggtctcct cttttccttg atgattggaa tttttagtat aggaattaca
     6781 aatacctatg cttcagaaaa ttctaatttc aatatcaact cccaaccaat tgaagaacaa
     6841 gcaattaatt ttgcgaaaac tgattttgaa cactccataa cttcaattct tttaatggaa
     6901 aataacaccg aaactgatat taactatact cttggttcgc catttaaaat gctcgatgga
     6961 gaaaatacgg caaatagttc tattttcttt ccagtatttt cagacgctga taatacgatt
     7021 aaatatattt ataccgtcaa aaaaaataca aatggagaat attcaggaag tatttcacaa
     7081 tttttagcaa aagaacttca aaatctagta gatagtaata taacagatac aaccttattt
     7141 tttcaagata atagcattta ttattccgat aatggacaaa ttaaactact ttggaaatca
     7201 cctttaagta cagaaaaaaa tggtgtttct tcctttacag aaaaaaatga tcttatatcc
     7261 gtaaatataa aaaataaaat taaaaatttc tctaaaaaac aaaattttcc tgcgattaaa
     7321 cgtgtaaaaa gtgtctcctc cggagataac tttattatga ttaattggcg tatagcagaa
     7381 acccaaacaa gttttccgtg gtgtgctgct tacgtagaag ctgctatctt atgtaacaaa
     7441 acagatgtac atgctacttc agctagagcc attatgcatt atacctatcc taatttaaac
     7501 aacagtcaaa ttgaatcaaa aagtcttaat caagatcaaa taataaaata tgcaaattac
     7561 tgtggtgttt atcctaaaaa agtagcacga atactaaacc tttcagaggt ccaaacacaa
     7621 ctaagaaaag gaaatgccat ttatatggga actgctggtt atggaaatta tagtaacagc
     7681 cgtcatgcac ttgcattatt tgggtgggtc aatcaaggtg gttatcaaac ttactatgtg
     7741 tggaatcctt ggtataacta cccaactgta ataagtgcaa atgcaggacc agttacaatt
     7801 cctgtacctg gaggtggttt tacttgggac aataccatta caaattggta aataaaccca
     7861 ttgctttagt tatgggtcta gtgttagttc ttttcttaac tagttgttca aaaaaacaag
     7921 aaatacaaaa gactgatctg attccttttc taggaacttg gcaaactaaa gatcattcag
     7981 aacgtctagt aatcactaat ttaaatactt caaaaaatat agaacaatat accatcttag
     8041 taaaagagaa caatcataaa attcaaatca ttgataaaaa tttaaatata aatgaacaag
     8101 ttttcattta caaagcaaca aataatttac taacactgcg ctctaataaa aaacaattag
     8161 attacacttt tagtttggat tctccaaaaa agctcgtatt tattttgaca acgaatgatg
     8221 ggacagaagg gacagctaaa ccaattgaat tttttaaagc tgattgatga atagttgttt
     8281 gatattacaa aacaataatt tcaccgaagg tgaaacaagc gaaactttac aaaaagtggc
     8341 tagcatttgc tagccttttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg
     8401 agcttgcgcc cctttgaaaa gcggttattt gcaccaaata cgtaggtatt tggctagcaa
     8461 atatagagct tgccaaaccc ttttagtgag ctttactcat tgtacctttt taggtacaat
     8521 ggtattgcga acgatacgtc cgtaggacgt actgaaaggg tgggacttat gagcgaacaa
     8581 aatcaaaatt tggctagcga tcattctaaa aaatatacct atcgatccga gccaaaacaa
     8641 atcagttttc gagtgagcga atctgaattt gcaaagctga agcagtcagc tgaagctttg
     8701 caaatgagtg tgccggcttt cgtaaaagcc aaggcacaaa acgcgcgtct ggtgacaccg
     8761 aaagtggcgc cagacattgc ccaggcaatc gcgcgcgatt tagccaaagc tggagggaat
     8821 atcaatcaaa ttgctaagtg gtgtaataca caccaacacg acgttgctcc tggagacgcc
     8881 caacgcttat ccgaaaacct aaaaatcatg caaaaggagc tacaaaagat atggcaacag
     8941 ttaaagtaag ccgaaccact tcttgtagtc gtgcaattaa ttacgctgaa ccacgagcaa
     9001 ccattaaaac aggaataaac tgtgacgtcg attatgccaa aagtgaaatg aaacaaattc
     9061 gcatgctgta tggaaaagac gaccgtgtac aggcacactt attgattcaa tcttttcgac
     9121 caggagaaat taccgctgaa aaagctaatc aactaggggc agagtatgca aaaaaaatag
     9181 cccccgatca ccaaatagct atttacacac atacagacaa agaccatatt cataatcaca
     9241 ttgtcattaa ctccgttaat ctggagactg gaaaaaaatt tcaagctcat ggtcaagcat
     9301 ttctaaacaa atgttatgac ctaaatgacg agatttgtct tgcacataac ttaccaatca
     9361 ctgagcgagg aaagaaacca gaaaaacgaa ccatgtctga aataaaatta aaagaaaaaa
     9421 atgaacctgt ttggaaagac gaaattcgga ttgcgatcga tcaaacaatg aaaaacaaaa
     9481 aaaccagaac ctatgaccaa ttttgtaatt ccctcaaaac attggggatt cattgcttca
     9541 atcgcgggaa aaatttcact tacgaattga tccacaaaaa gaaaaaagta cgatccaaca
     9601 gactaggaaa ggactacgaa aaggagacga ttttacatga gctggacaga cgagaacgaa
     9661 cctataacca acagcgaacc aacgagctac gaagagctcg agctagcatt cgcgaaactg
     9721 cagaagaagt acgaagagaa caacgaactc ttacgcctga acaaccggaa attagaccgg
     9781 ctcgaagaaa ccatgaacaa cacgttgaaa gaggaacaga ccaccaacaa gaattatcgc
     9841 tctaaaacgt taaaagacat agaaaacgaa ataaagaaac aacgtgcttt tctcgttcct
     9901 tatacgcaag aaaggataca aactacgatt gatttacaag ttaaaaaact ccatcaagag
     9961 ctattagacc agcgaaataa ggaacagaaa aaggtgctta ttcaacgatc attggatttt
    10021 cttgccccct tatgttgctt agtcctgaca ctgttcgcat tttggttagt atggggaggc
    10081 taaaatggca ttttatttac cctatctact catttttgtt agtatttcag gaagtatttg
    10141 gctcatctat aaaatttttc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa
    10201 gcgatttttc tttcttggct gtatattttc cttgataatc gtttcaagcg gcttattcgg
    10261 cgttttagag ggaaataagc gcgtttctag aagtatttta ttaggaaatg tcactcaaaa
    10321 gtatgaatca gcgcgtgata aaaagaaaaa agagcaagta ttagcacaaa aaatagaaaa
    10381 atttaccgct tgttatgagg acatgaatga tatttttgtg aagcaagaaa agcgtttgac
    10441 ggataaaaat atggaaacac tcactcgact ttatcggaat ttgccagaag aacagcaaaa
    10501 agagtaccaa gaaaaatatg aacaagtaaa aaaagacgtg caatatgtga aagatacgaa
    10561 agtggaagaa gcttgtagtg atttatttgg agatacaatt cctttttcaa ctagcgaaca
    10621 agaacgaaaa gaacgacaac aatcagtcac atatgaacgc tatgaaatct tgcttcaaca
    10681 agcaattaac atacaaaatc tgactaaaaa agaaacagct ttaaattatt taaaaagcgt
    10741 caaggaatgg ttagatcagc aacagcaaaa ttgaatttga ttgaaattca tgttaaacta
    10801 aacatgcaaa tgttcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg
    10861 gcttttttta ggttgatgtc gcctttactg attttacttc ttgtttcggt tattcaacca
    10921 ataactgaat acagagatac aaactcctac aagaaggggg caaataaaca gttcaaacat
    10981 cagatacacc tccttccagt cgcaagactg tcggataggc gacaagttat tataccctac
    11041 aaaattaaat ctgtgtggca attttaagta tctcttattt ccgggttgtt agggcttgcc
    11101 ctgaccgcct gtagggcgct ttaaatgcca gataaaggtt tttatactaa gactgtctta
    11161 aaacggctta gacaagctct tagagcctca accctccttt ttgaacaatg gttttagcaa
    11221 aggagctgga ggcgactgcg caggtataat gtgagagcgt agcgatcaca cctttttggc
    11281 gaccgtaggg agcgaggttt tgaattagca gtttagaaaa gttcgcgaac ctgtttaaag
    11341 aaaaaataat tgttttttta ggtgggaaat tttgtctccc tcttttataa tcttttttta
    11401 aacctctttt taaacctctt taccgtcggc tgtgtcctta ctcccccaag ggatacaaaa
    11461 ttttataggc atgaaattat ctgtttatag gcatgaaatt atctgtttat aggcatgaaa
    11521 ttatctgttt ataggcatga aattatctgt tttaggtatt gtttttagac ataatatgtc
    11581 ctaaaatgat gtcaataaaa tcatttagac tggagtaaca actaa
//
