LOCUS       NZ_CP084106             4820 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae strain C11 plasmid pC11-4kb, complete
            sequence.
ACCESSION   NZ_CP084106
VERSION     NZ_CP084106.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21211508
            Assembly: GCF_020459305.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Liu,Z. and Chen,R.
  TITLE     Epidemiological and genomic study of blaKPC-2 or blaNDM-1 positive
            Klebsiella pneumoniae isolates
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Liu,Z. and Chen,R.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2021) Veterinary, Yangzhou University, 88
            University South Road, Yangzhou, Jiangsu 225009, China
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Method                   :: Unicycler v. v0.4.8
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 100.0x
            Sequencing Technology             :: Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:02:58
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,324
            CDSs (total)                      :: 5,204
            Genes (coding)                    :: 5,068
            CDSs (with protein)               :: 5,068
            Genes (RNA)                       :: 120
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 86
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 136
            CDSs (without protein)            :: 136
            Pseudo Genes (ambiguous residues) :: 0 of 136
            Pseudo Genes (frameshifted)       :: 56 of 136
            Pseudo Genes (incomplete)         :: 84 of 136
            Pseudo Genes (internal stop)      :: 32 of 136
            Pseudo Genes (multiple problems)  :: 31 of 136
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084106.1.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="C11"
                     /isolation_source="sputum"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pC11-4kb"
                     /geo_loc_name="China: Zhengzhou"
                     /lat_lon="36.14 N 112.42 E"
                     /collection_date="2018-05-31"
                     /collected_by="Ruifei chen"
     gene            complement(join(4605..4820,1..171))
                     /locus_tag="K8M04_RS26580"
                     /old_locus_tag="K8M04_26505"
     CDS             complement(join(4605..4820,1..171))
                     /locus_tag="K8M04_RS26580"
                     /old_locus_tag="K8M04_26505"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045282373.1"
                     /translation="MRISNVEWLKKRIAFIRKLGEKTARQRQIIDLLDDEASLSETERR
                     LLHVLATAEKNALQERDAAQKKAVQARISGQKNRRERNHRLFLAAGLMIDAGLVDTKTG
                     ELNFDHEKLLTALKRVRYDLETSA"
                     /name="hypothetical protein"
     gene            338..1273
                     /locus_tag="K8M04_RS26585"
                     /old_locus_tag="K8M04_26510"
     CDS             338..1273
                     /locus_tag="K8M04_RS26585"
                     /old_locus_tag="K8M04_26510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="WP_045626452.1"
                     /translation="MATYHLSVKFGGKGKALAHASYITREDKFSQRQDLEHTEHGNMPE
                     WARDEPAHFWQAADAFERANGSTYREIEIALPRELNEAQRLALVRDFVKQETGDKHAWT
                     FAIHNPKASIDGGEQPHAHIMMSQRVNDGIERSPEQYFRRYNAKFPARGGARKDSGHNL
                     TPTEQKQALKALRERWERTHNAHMKRYGFEQGFIDSRSLKTQGIDRKPEQHLGFEAAGR
                     LDDSQRQDIKDSRTFSVVDVPEQAKHIDHSDRSWMNASGIEVNRKELVRSLTHDISQQY
                     GREMAARVTKQGNVEIERVKSLSRGFSISR"
                     /name="MobA/MobL family protein"
     gene            1276..1806
                     /locus_tag="K8M04_RS26590"
                     /old_locus_tag="K8M04_26515"
     CDS             1276..1806
                     /locus_tag="K8M04_RS26590"
                     /old_locus_tag="K8M04_26515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_012634455.1"
                     /translation="MASTEEKLLTLLLSAEHLKEAAKEQQKAVADSLAVARETLKGYRE
                     ISDSVAQQVRDEVRQEVQKMDVAGLIGERISADFQRLEKSVGNMKINADALDKEISIIR
                     SNIIAEYNTLRGYSWKWFAGVAVAFVVLIFATSWFVKTELQDNYNASAAVYQKVADLEK
                     LLTTEQKPEKKKN"
                     /name="hypothetical protein"
     gene            complement(2836..3533)
                     /locus_tag="K8M04_RS26595"
                     /old_locus_tag="K8M04_26520"
     CDS             complement(join(2836..3284,3284..3533))
                     /locus_tag="K8M04_RS26595"
                     /old_locus_tag="K8M04_26520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558267.1"
                     /ribosomal_slippage=""
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="WP_223433957.1"
                     /translation="MASVSISCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFTY
                     TASQPGTHQKIIDMAMNGVGCRASARIMGVGLNTILRHFKKLRPQSVTSRIQPGSDVIV
                     CAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLSTLERLLGLLSAFEVVVW
                     MTDGWPLYESRLKGELHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELHDKVI
                     GHYLNIKHYQ"
                     /name="IS1 family transposase"
     Typing          339..1270
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.177"
                     /coverage="100.0"
                     /evalue="0.0"
ORIGIN
        1 cgcgtttttt tcagcggttg ccagaacgtg gagcagtcgt ctttcagtct cactgagcga
       61 agcttcatca tccagcagat cgataatctg ccgttgccgt gccgtttttt ctcccagctt
      121 tctgatgaag gctatgcgct ttttaagcca ttcgacgtta ctgatccgca tcgtttaact
      181 tccccatccc gatatttccg acggggaaag tatataactt gcggtcagaa gtcgcatgac
      241 ttatctttac tcataattct ttaaattaga gtaaagcgca cttatatgtt tctccctgcg
      301 ggcagaaaca gtgcgccctg ccgggggctg acacaacatg gcaacgtatc atctcagcgt
      361 gaaatttggc ggcaagggta aagcccttgc tcatgccagt tacattacgc gggaagataa
      421 gttcagccag cgccaggatc tcgagcacac cgaacacggc aatatgccgg aatgggcgag
      481 agatgaaccc gcccatttct ggcaggctgc ggatgcgttc gaacgtgcca acggttcaac
      541 gtacagggaa attgagatcg ctctaccccg cgagctgaat gaagcgcagc ggctggcgct
      601 tgtccgggat tttgtgaagc aggaaacggg cgacaaacat gcatggacgt ttgcgatcca
      661 caatccgaaa gccagcattg acggcggcga acagccgcac gcccatatca tgatgagcca
      721 gcgcgtcaat gacggtattg agcgcagtcc cgaacagtat ttcaggcgct ataacgccaa
      781 atttccggcg cgtggcggcg cgcgcaagga ctcagggcat aatttaaccc ctacagaaca
      841 aaaacaggca ctgaaagccc tgcgggagcg ctgggagcgt acgcacaacg cgcatatgaa
      901 acgatatggc tttgagcagg gttttattga tagccggtca ctgaaaacac agggtatcga
      961 ccggaaaccg gaacagcatc tcggttttga agcagccgga cgactggacg acagccagag
     1021 gcaggatatt aaagacagcc ggacgttttc agtcgtggat gtgcctgaac aggcgaaaca
     1081 tatcgaccac agcgaccgta gctggatgaa tgccagcggt atcgaggtaa accggaagga
     1141 actggtcaga tctctgacgc atgatatctc gcagcagtac gggcgggaga tggctgcacg
     1201 ggtcacaaaa cagggaaatg ttgaaataga gcgcgtgaaa tccctgtcac gcggattttc
     1261 cataagcagg taactatggc gagcaccgaa gaaaaacttc tcacactcct gctgtcggcg
     1321 gagcatctga aagaggccgc aaaagagcag cagaaagccg tggcggattc gctggcggtg
     1381 gcaagggaaa ccctgaaagg ctaccgggaa atatcggaca gtgtggcaca gcaggtaagg
     1441 gatgaagtcc ggcaggaagt gcagaaaatg gatgttgcgg ggctgattgg cgagcgtatc
     1501 agcgctgatt ttcagcgtct ggaaaaatcc gtcgggaaca tgaaaatcaa tgcggacgcg
     1561 ctggacaagg aaatttccat tatccgcagt aacatcatcg cggagtacaa cacgctgcgg
     1621 ggttacagct ggaagtggtt tgctggggtt gcggttgcct ttgtcgtact gatatttgcc
     1681 acaagctggt ttgtgaaaac ggaactacag gacaattaca acgcgtctgc tgccgtctat
     1741 cagaaagtgg cagaccttga aaagctgctg acaacggaac agaaaccgga aaagaaaaag
     1801 aactgacgcg ctgcgcttgt ccaacgcctc acgttcgcac aataaattgc gcacccgttc
     1861 accgttttca gccgagtttg gggcgtcttt tgagacttcc ggcctgaatt caggccggaa
     1921 gttctcgcca gtcagtcagg tgcatggtta tgcatggggc tgaaaatcac ggtatgcgat
     1981 tctgagcggt tttcggtgag aagccggggg tttttatggc tcaggacgta aaaccgccct
     2041 gagcgcgttt cagcggtgcg cgtaacggat gttatggtaa atccggtggc tttgacgtgc
     2101 ctgccgttgt gtaccgtcgt gagtgaaaat aattatattg ttcgaagaac agcgcttttt
     2161 ggccggagaa aagcagttct gcaaacgatg gtatatcgat atgagttctt ttggcattaa
     2221 tccaggcaag acgccgggca ataagcgtaa tattccggat gttacttatt tccatttctg
     2281 tcaacagcat ccccccccac gcccatcccc ccccacgccg gagaataaaa aagccgcctg
     2341 ttccatcggc aagccctaca tcaagggaaa ctacgtctta cgattcgttt tgaaccggga
     2401 cgctttgttg ctgcgaatca ctaaccagat aatccacgtt ttgtgagtgg gtttaggtta
     2461 aggcttcaat agaacaataa tagcgttaag tgattagggc aacctgtgat tgctaaaatg
     2521 atgaagtctt cacatcattt cggtgaaggc gatatgtgtt acaccacgta ttgagagacc
     2581 tgcatgccct ttacaggtga acgggaacgg gaatagagcg gcgatttagc cagagatggg
     2641 tttcataaca ctgtaattca tatcacaatt attataactg gcggtatttt tttaaccttt
     2701 gcaattcgtt ttatctgatg tttatgataa ttttcaaaaa gttggccccc tgacatcacc
     2761 gggagccaaa aaaaaataca ccagttatta ttgtggctat attatttcat gagtaaacaa
     2821 ggtaatgact ccaacttact gatagtgttt tatgttcaga taatgcccga tgactttgtc
     2881 atgcagctcc accgattttg agaacgacag tgacttcctg cccagccttg ccagatgttg
     2941 cctcagattc aggttatgcc gctcaatgcg ctgcgtatat cgcttgctga taacgtgcag
     3001 ttctcccttc aggcgtgatt cataaagcgg ccagccatcc gtcatccata ccacgacctc
     3061 aaaggccgac agcaggccca gaagacgctc cagcgtggac aacgtgcgtt caccgaatac
     3121 gtgcgccaca accgtcctcc gtatcctgtc atacgcgtaa aacaaccagc gctggcgtga
     3181 tttagcgccg acataacccc actgttcgtc catttccgcg caaacaatga cgtcactgcc
     3241 cggttgtatg cgtgaggtta ccgactgcgg tctgagtttt ttaagtgacg taaaatcgtg
     3301 ttgaggccaa cgcccataat gcgtgcactg gcgcgacatc cgacgccatt catggccata
     3361 tcaatgattt tctgatgcgt accgggttga gaagcggtgt aagtgaactg tagctgccat
     3421 gttttacggc agtgagagca gagatagcgc tgatgtccgg cagtactttt accgttacgc
     3481 accacgcctt cagtagctga acaggaggga cagctgatag aaacagaagc cactggagca
     3541 cctcaaaaac accatcatac actaaatcag taagttggca ccattaccgt aaacaaatat
     3601 gttaggcgta tatttgttta ctcaaattgc ttgtttttta gtcatcgtga tgatgagcga
     3661 gagaaaatcg atgttgatag gttttttatg cttttgattt ttttatatgt ttgtattatt
     3721 taaactgttt ttctgtattg tgtagcattc cctttagatt tttatgccat cgtcgccgat
     3781 ggctttttta ttccgttaag gtaatttttg agttcatttg catgaacgac tgatgttctc
     3841 catgttcgtt ttcactattc tttttctccc gcgaatttga tttatgtggt tgtgacatta
     3901 gttatgagat agagccacca gtgtgatgaa ctcacactac aaattcgttc agcactaaat
     3961 cagaattcga tgcaaatctc ggacgctgga tcatcacagt gaatccttct ataacccgga
     4021 tttgagagtc gcaaacccat attagggcaa agaaagccta atgcccccgt ggtgggtaca
     4081 ccagaaaacg gggagcgaag cgaccagcat caggccgttc gtatgctgct tcgattttgt
     4141 gggtgttgcg ccagaccttg gcaatcacgc ttcaccgcaa ccgctgcctt cactctcact
     4201 ttatcaggta cgttattggt taacgtgaga ccgggtagag ccagcttctt tcccgagctg
     4261 gtcaggttcc caccgcttgc ctgtttatcc tgccggagtc cggggcgtag acctgagagt
     4321 caaggtgcag atatgggttt aatgtcgttg aatggtatta agctggtagt gacattccta
     4381 gccagccctg ctaggatttg attgtgacgt ctgatcctgg caggtatctg caagaattag
     4441 agtactcaag ccccgtgatt ctgtcacggg gcttttgtac atctggagca agaaaattca
     4501 tgctcctatc cgaaaatccc taccgctcgc cgcagccgaa cgaccgagcg acgcgagtca
     4561 gtgagcgagg aagcggcatt tctccggcct tgcagaatgc acgttcacgc cgatgtttcc
     4621 agatcgtagc gtacccgttt cagcgcagtc agtaattttt cgtggtcaaa attcaactca
     4681 cccgtttttg tatcaaccag tcccgcgtca atcatcagac cagccgccag aaacagtctg
     4741 tgattgcgtt ccctgcggtt tttctgccct gaaatccgcg cctgaaccgc ctttttctgt
     4801 gcggcatccc gttcctgcaa
//
