LOCUS       NZ_CP084053             2540 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P9, complete sequence.
ACCESSION   NZ_CP084053
VERSION     NZ_CP084053.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084053.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P9"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(<2539..2540,1..1450))
                     /locus_tag="KWJ12_RS27080"
                     /old_locus_tag="KWJ12_27080"
                     /pseudo=""
     CDS             complement(join(<2539..2540,1..1450))
                     /locus_tag="KWJ12_RS27080"
                     /old_locus_tag="KWJ12_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            complement(1454..2014)
                     /locus_tag="KWJ12_RS27085"
                     /old_locus_tag="KWJ12_27085"
     CDS             complement(1454..2014)
                     /locus_tag="KWJ12_RS27085"
                     /old_locus_tag="KWJ12_27085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001161490.1"
                     /translation="MQGHRIGYVRVSSFDQNPERQLEQTQVSKVFTDKASGKDTQRPQL
                     EALLSFVREGDTVVVHSMDRLARNLDDLRRLVQKLTQRGVRIEFLKEGLVFTGEDSPMA
                     NLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKALSDEQAATLRQRATAGEP
                     KAQLAREFNISRETLYQYLRTDD"
                     /name="recombinase family protein"
     gene            complement(2190..2390)
                     /locus_tag="KWJ12_RS27090"
                     /old_locus_tag="KWJ12_27090"
     CDS             complement(2190..2390)
                     /locus_tag="KWJ12_RS27090"
                     /old_locus_tag="KWJ12_27090"
                     /inference="COORDINATES: protein motif:HMM:NF023237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="WP_000993264.1"
                     /translation="MNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQA
                     RASTATETSVKPDACDSPPSG"
                     /name="DUF3330 domain-containing protein"
     gene            complement(2377..2496)
                     /locus_tag="KWJ12_RS27235"
     CDS             complement(2377..2496)
                     /locus_tag="KWJ12_RS27235"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_267461513.1"
                     /translation="MVAAGGLRHGAGFPVRQADAGGGLRRLRQPMEEHHHERQ"
                     /name="hypothetical protein"
ORIGIN
        1 cgcggaactg ccgcgagccc ttgacccaga tgtcgccgga gcgcagggcg ttcttcagct
       61 cggacagggc gcagatttcg tagaatttcc ggtcgaggcc ttccggggtg atcaccagcg
      121 gcttccagcg cggcttgatg aaggccgtgg gtgcatcggc cggcaccttg cgcaggttgt
      181 cggcgttcat ctcacgcagg gtctgcacgg ctgccagcac gccttgcgcg gccggcgcgg
      241 cgcgcagttc cagcacctcc agcaaggccg gcgtgtaacg gcgcagggtg gcgaagttct
      301 cgccgaccag gtgcaggtgg tcgaagcctt ccggccgggc cagcagctcg gcctcgctga
      361 cgctctcggt gaactcgtcc cagggaatca ccgcctcgat ggcggcatag gggtcgctgc
      421 cgctttcctt cgcttccagc agcgcctggc cgatcctgga gtacaggcgc accttgtcgt
      481 tgatcgcctt gccctgcttc tggaactgct gctgatgctt gtgcttcgcg ccgctgaaca
      541 gcttgaccag gatgcggtca tgcagatcga ccagctcatc gatcacggtc gcggtgctct
      601 ccagcaccac ggcggccagg gtcgcgtagc ggcgctgcgg ctcgaactta ccgaggtctt
      661 tgggcgtcat ctgcccaccc tcgcgggcca gcttgagcag gcggttctgg tggatgtgcc
      721 ggcccaggcc ttcgggcaag tccaccaact gaaatgtctt cagccgctcg atgtgttcca
      781 gcatgtgccg agagttgggt ttcagcggtg cctggcgcag ccaggtcaac caggtgatgc
      841 tgctgccggc cttgagcttc aacagctcgt ccagcttggc ccgatgcgag tccgtgagtg
      901 gttcgaccag ggcgcggtag acccgccgat tggctcgcgc aatggcttcc gagcaggccc
      961 ggtcaatcac gctcagcgcc ggcaggatgc gtcgtttctg ccgtaggctc tccagggcct
     1021 gaccggccag cagcaagcct ttgtcggtct gctgggccag ctcggttagc tcgcgcacca
     1081 gggcgcggaa gtcggacagg ccgaacgggg ccagttgcag gtaggtgcgc agttcctggg
     1141 catgctcgcg acgggtcacg tcgcgctcgc cgtactttgc ccagctcgcc ggctcggcct
     1201 ggacttgctt cgccacccac aggatgaccg gctcgggcag ctcgctgtcg gttcccagcg
     1261 cgtaaccggg gtagcgcagc aggcagagct gcacggcgaa gccgaggcgg ttggcgtcgc
     1321 cgcgtcgctg gcggatcagc gacaggtcgg agtcgttgaa ggtgtagtag cggatcaggt
     1381 catcctggct ttccggcagc gcaagcaggg tgtcccgctc cgtggccgag aggatcaagc
     1441 gacgcggcat gtgtcagtcg tccgtgcgga ggtactggta gagggtttcc cggctgatgt
     1501 tgaactcgcg ggcaagctgc gccttgggct cgccggccgt cgctcgctgc cgcagggtag
     1561 cagcctgctc atcggacagg gctttcttgc ggccccggta cgcgccacgc tgcttggcca
     1621 aggcgatgcc ctcacgctgc cgctcgcgga tcagggcgcg ctcgaactca gcgaaggccc
     1681 ccatcaccga cagcatcagg ttggccatcg gcgagtcctc gccagtgaac accaggccct
     1741 ccttcaggaa ctcgatgcgc acgccgcgct gagtcagctt ctgtaccaag cgacgcaggt
     1801 catcgaggtt gcgggccagc cgatccatgc tgtgcaccac cactgtatcg ccttcgcgga
     1861 cgaagctcag cagcgcttcg agctgggggc gctgggtgtc cttgcccgat gccttgtcgg
     1921 tgaacacctt gctcacctgg gtttgttcca gctggcgttc cgggttctgg tcaaagctgc
     1981 tgacccggac gtagccgatg cggtgcccct gcacgatgtc tccttggttg aaggcggctt
     2041 aagtgcactt tctgttccgt tgtgcctcaa agcccatttc tgtcaggctg aaatctataa
     2101 ccttcgcggg catgtgtcaa aaaatgggaa agcagactct attctgacga agcggcgcgg
     2161 ccctgcctga catcaagtta gggtatgcct caacctgacg gcggcgaatc acaagcgtcc
     2221 ggtttgacgc tggtttcggt cgcagtgctg gcccgcgcct ggaagcgctg atagcactcc
     2281 agcccgcaga aatgctccac gtactcggcc ccttccggcg tgaaggcggc atcgagcggg
     2341 atttccttgc agcacacgca gcaactggtg ctcggttcat tggcgttcat ggtggtgttc
     2401 ctccattggt tgacgaagcc gacgaaggcc gccgccggca tcggcctggc gaacaggaaa
     2461 ccctgcaccg tgtcgcaacc cgcctgccgc aaccacgcaa ggcagtcggg tgtttccaca
     2521 ccctcggcta ccacctccat
//
