LOCUS       NZ_CP084052             2828 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P8, complete sequence.
ACCESSION   NZ_CP084052
VERSION     NZ_CP084052.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084052.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P8"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(2802..2828,1..1080))
                     /gene="mobV"
                     /locus_tag="KWJ12_RS27070"
                     /old_locus_tag="KWJ12_27070"
     CDS             complement(join(2802..2828,1..1080))
                     /gene="mobV"
                     /locus_tag="KWJ12_RS27070"
                     /old_locus_tag="KWJ12_27070"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobV family relaxase"
                     /protein_id="WP_218423645.1"
                     /translation="MYSIIRKQSHKKTAIQAVHNHNMRIVIEENVNSKKSNLNEILIGT
                     ENTRSDIMSYIKNNDIVIRNKDTVIFNEFVLTASPEFFFNNKDGSKKTKSEYNDNLNEW
                     VKTQIEFLEKENYGKCVNAVLHLDESTPHIHALILPIRDNKLNNKSFWRGKNSYGRLQD
                     LYNASNSKFGLKRGEEKSKTLVDHTTLKDYRELIKQDLEEEKEFYNELSKDILDIPPRK
                     NFIGLEKKYSAEEVKEIASATFKKLNRQRRRAKFRAKKSDEKADAATSEFYKKKKETSK
                     IEFFYNENREKLEKISVENSTLKTQNLELKQSQSDLDLVRKFLPMELQSLVVRARELAS
                     GGDSGGTKHTAEPTSTADLTTYKRPKYK"
                     /name="mobV"
     gene            2050..2784
                     /locus_tag="KWJ12_RS27075"
                     /old_locus_tag="KWJ12_27075"
     CDS             2050..2784
                     /locus_tag="KWJ12_RS27075"
                     /old_locus_tag="KWJ12_27075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_142428737.1"
                     /translation="MTIQEVKKPRVRTKTENDKLTEYKMNPFVFDKELKIETKTRNLTV
                     RKGTELVNKDNADESESYFSNIVQKKEVDKEEFIKLFTSQIKVYFDLTKTAHKIFLIIL
                     ALYQKEIGKDYVLLTCKKAQNVAKTLDFELSAPIFYRGIKELVEKKIIAKSVDKIVFYI
                     NPAVFFNGDRARFVTEVIKKKEEIEKKQFLEELETTSKNKDFETDDSVENLDLMIARLQ
                     KKKEELERKEKFGQWQDLEDAS"
                     /name="hypothetical protein"
ORIGIN
        1 agttagatct gcagttgatg taggttctgc agtatgcttt gtaccgccag aatcgccgcc
       61 agaggccagt tctctggctc tgacgacaag gctttgcagc tccataggta aaaattttct
      121 caccagatct aggtcacttt gtgattgctt aagctctaag ttttgggttt taagtgtgga
      181 attttccaca gagatttttt ccaatttctc tcggttttca ttataaaaga actcaatttt
      241 tgatgtttct tttttctttt tataaaattc acttgtagct gcatctgctt tttcatcaga
      301 ttttttagct ctaaattttg ctcttcttct ttgtctattt agttttttaa aagtagctga
      361 agcaatttct tttacttctt cagcagagta ttttttttct aagccaataa aattttttct
      421 tggtggaatg tctaatatat ctttcgataa ctcattataa aattcttttt cttcttctaa
      481 atcttgtttg attaattctc tgtaatcttt tagtgttgta tgatcaacta aagttttact
      541 tttttcttca ccacgtttta aaccaaattt actatttgat gcattatata aatcttgtaa
      601 tcttccataa gaatttttac ctcgccagaa agatttattg tttaatttgt tatcacggat
      661 aggtaaaatt aatgcatgaa tatgaggtgt tgattcatca agatgcaaaa ctgcattaac
      721 acattttccg taattttctt tttctaaaaa ctcaatttgg gttttaaccc attcgtttaa
      781 attgtcatta tattcagatt tggttttttt tgaaccatct ttattgttaa agaaaaattc
      841 tggtgatgca gttaaaacga attcgttaaa aattactgtg tctttatttc taatgacaat
      901 atcattattt ttaatataac tcattatgtc agatcttgta ttttccgtac caattaatat
      961 ttcattcaaa tttgattttt tagaatttac attctcctct ataactattc tcatattatg
     1021 attatgtaca gcttgtatag ctgttttttt gtgtgattgt ttcctgatta ttgaatacat
     1081 ttttatttct ccatattaat atacaacccc catttttttg gataaatcca atattatgag
     1141 aatgtcggaa acattctcat gtggcgttaa atgccacctg tgcagctgtg taaggttttg
     1201 acgttgaacc ggtgcaaacc ggttcggggc ttgggtgggc tatgcccccc aacgaacgac
     1261 tgagcgtagc tcggtctagt gagtgataga ccgaaagtaa actcaacgta tatatttaaa
     1321 gttatcactt taatatattc tgtcaaatag ctaccgcctc gcctaacggc tcgttgctga
     1381 atccttttta aagcaaaagt aaacttttac tttaaaaatg attgatcaca ataataataa
     1441 aatacatgaa caaaaaagac gggttgcgat gtgggcaagt tcttgaccac tcgcaggggt
     1501 ggggggaccc ggaacaagag tacttgttct ggtgggggga acgggtttag gggttccccc
     1561 cagtagctgt agaacgctct ctaacgctct atgacgggtt tttgtagtta cttaaaccgt
     1621 gagagtccac gtacagcttg ctgtatgtgg attcgagggg ttggcctgcc cggcgagggc
     1681 ggttttagat tttgatttta ctcttagatc ttaatattta ttaattcttg cagaaaaact
     1741 caaatcctaa aactcctctc taagccttgg tacgtctggt tttactccat tttttctaaa
     1801 aaacaaaaag tacaaagttt tatactctat ccttaaaaaa gtaacgatag agtaataagt
     1861 tttatactct agagtaataa actttatcgt tttgcaccca taaaaacgat aaacaggcta
     1921 aaatttctag aattttattt atcgttttaa gcctcaaaaa cgattttttt ttatcgtttt
     1981 gtattgactt gtttaaaaaa tcggttataa cgctagtatt aagtatctaa atttaaagag
     2041 gaataagtta tgacaataca agaagtaaaa aaaccaagag ttagaacaaa aacagaaaat
     2101 gataaattaa cagaatataa gatgaatcct tttgtttttg acaaagaact aaaaattgaa
     2161 acaaaaacaa gaaatttaac tgttagaaaa ggtactgaat tagtaaataa agataatgct
     2221 gacgaatcag aatcctattt ttctaatatt gttcaaaaga aagaagtaga taaagaagaa
     2281 tttataaaat tatttacatc tcaaataaaa gtatattttg atctaacaaa aacagctcat
     2341 aaaattttcc ttataatttt agctttatat caaaaagaaa tcggtaaaga ttatgtttta
     2401 ttgacatgta agaaagctca gaatgttgct aaaacattag attttgagct ttcagcacca
     2461 attttttatc gcggtataaa agaactggta gagaaaaaaa ttattgctaa atctgtagat
     2521 aaaattgttt tttatataaa tcctgcagtt ttctttaatg gtgatagagc aagatttgta
     2581 actgaagtta tcaagaaaaa agaagagata gagaaaaaac aatttctcga agagttagaa
     2641 actacatcaa aaaataaaga ttttgaaact gatgatagtg ttgaaaattt agacctgatg
     2701 attgcaagat tacaaaagaa aaaagaagaa ttagagcgaa aagaaaaatt cgggcaatgg
     2761 caggatctgg aagatgcatc ataaaagccc tgatgggctt tttatttgta tttaggtctt
     2821 ttataggt
//
