LOCUS       NZ_CP084051             2921 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P7, complete sequence.
ACCESSION   NZ_CP084051
VERSION     NZ_CP084051.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2921)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084051.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P7"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(<2..553)
                     /gene="aac(6')-Ib"
                     /locus_tag="KWJ12_RS27225"
                     /pseudo=""
     ARG             complement(3..553)
                     /name="aac(6')-Ib"
                     /gene="aac(6')-Ib"
                     /gene_name="AAC(6')-Ib family aminoglycoside
                     6'-N-acetyltransferase"
                     /tool="amrfinderplus"
                     /drug_class="AMINOGLYCOSIDE"
                     /identity="100.0"
                     /coverage="100.0"
     gene            1172..2527
                     /gene="ltrA"
                     /locus_tag="KWJ12_RS27065"
                     /old_locus_tag="KWJ12_27065"
     CDS             1172..2527
                     /gene="ltrA"
                     /locus_tag="KWJ12_RS27065"
                     /old_locus_tag="KWJ12_27065"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010368123.1"
                     /GO_function="GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /GO_process="GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="WP_015613264.1"
                     /translation="MNLNSHRSPCCDSDEQRSTVNNTSNEYNQIDHDLMAKVLSNHNIS
                     AAWQHVKRNKGAAGIDNMSIEEFNDFAKLHWLGIKQQLLNGSYQPLPVKRVMIPKPDGG
                     ERMLGIPAVIDRVIQQAIAQVISPYFEPQFSPHSYGYRPHKRASQAVNHVQSCVKQGYK
                     TAVDIDLSKFFDEVDHDMLMNRVGRKIKDKALMRLLGKYLRAGIAERETGLWFESTKGV
                     PQGGPLSPLLSNILLDELDKKLTYKHLKFARYADDIIILVKTKSEGLIIQREITAFITK
                     RLKLKVNESKSRVGPVSGSKFLGFTFRYGQVQIHEQALKKFKANVRELTNRNWGISMTL
                     QIHKLTQYLRGWGHYYLIANAYQLTVDLDHWIRRRIRMCYWRQWRKPRTKVRSLMKLGV
                     SERLAIACGITSKGPCRSSKTKGINIALGNNYLASQGLVSLRDIWINIHYGR"
                     /name="ltrA"
     gene            complement(2608..>2919)
                     /gene="arr"
                     /locus_tag="KWJ12_RS27230"
                     /pseudo=""
     CDS             complement(2608..>2919)
                     /gene="arr"
                     /locus_tag="KWJ12_RS27230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /name="arr"
     ARG             complement(2609..2919)
                     /name="arr-2"
                     /gene="arr-2"
                     /gene_name="rifampin ADP-ribosyltransferase (Arr)"
                     /tool="rgi"
                     /drug_class="rifamycin antibiotic"
                     /identity="100.0"
                     /coverage="68.67"
ORIGIN
        1 aggcatcact gcgtgttcgc tcgaatgcct ggcgtgtttg aaccatgtac acggctggac
       61 catctggggt ggttacggta ccttgcttct caaaccccgc tttctcgtag catcggatcg
      121 ctcgcaagtt gctcggcgac gggtccgttt ggatcttggt gacctcggga tcattgaaca
      181 gcaactcaac cagagctcga accagcttgg ttcccaagcc tttgcccagt tgtgatgcat
      241 tcgccagtga ctggtctatt ccgcgtactc ctggatcggt ttcttcttcc caccatccgt
      301 ccccgcttcc aagagcaacg tacgactggg catacccaat cggctctcca ttcagcattg
      361 caatgtatgg agtgacggac tcttgcgcta aaacgcttgg caagtactgt tcctgtacgt
      421 cagcaagtgt cgggcgtgct tcttctccgc cccaccactc gacgatatga gatcgattta
      481 gccactcata gagcatcgca aggtcatgct cagtcatgag gcgcagtgtt acggaatcgt
      541 tgctgttggt cacgatgctg tcctttgtga tgcctaacgc cagaattaag ccgtgccgcg
      601 aagcggcgtc ggcttgaatg aattgtgtac gcccgacatg ggcattgact tatgaggtga
      661 aagtcctctg taggaagatc accgtttaaa taacatactg ttattaaacg ataactacta
      721 gcgaatggca agggctaaat cgcgagagat agtctgaagg aagccgctag caaatctgtg
      781 agctgatgaa caaaaacatc atatgaggcg taggctggag acgagctagc acaagatagc
      841 gaagtcatgt gatctaacgg ccaccgtaaa tgatgcagtt gtgcagagaa agtcgatgca
      901 tcttattcgg ggagatctga taaacacgca tccgcgctaa cgcttggaga ttaccagtta
      961 gggactggtt gttcaaccct tggcgctttg gttgctgcga cctacagcga gcgaaatagc
     1021 agtaaacaag cgttagtttt agacgcgctt tatcgagtaa tcgctagagt gatttatcag
     1081 aagtcagcag acggcatagt agccaaacgc ccatcgtaat ggaggggaca cggtgaaggc
     1141 ctgaacatct taaccagagg agacactaca aatgaacttg aattcacata gatcaccgtg
     1201 ttgtgatagt gatgaacagc gttcgactgt gaataatacc tcgaatgaat ataaccaaat
     1261 agatcatgac cttatggcaa aggtactaag caaccacaat attagcgcag catggcaaca
     1321 tgttaagcgc aataaagggg ctgcgggtat tgataatatg agtattgagg aatttaatga
     1381 cttcgctaag ttacattggc tgggtattaa acaacaatta cttaacggca gttatcaacc
     1441 cttgccagtc aaacgcgtga tgatacctaa acctgatggc ggtgagcgaa tgcttgggat
     1501 ccctgctgtt atcgacagag tgatccaaca agccattgct caagtgatca gcccttactt
     1561 tgagcctcaa ttttcaccac atagttatgg ttatcgccct cataaaagag ccagtcaagc
     1621 agtgaaccat gttcagtctt gtgttaaaca aggttataaa acagcggttg atatcgactt
     1681 gtctaagttc tttgatgaag tagaccatga catgctgatg aatcgtgttg gccgtaaaat
     1741 caaagacaaa gcactcatgc gcctactcgg taaatactta cgagcgggga tcgcagagcg
     1801 cgaaacaggt ctgtggtttg agtcaaccaa aggagtccct caaggtgggc cattatcccc
     1861 tttgctttcc aacatcttac tcgatgaatt agataagaag ctaacctaca aacaccttaa
     1921 gtttgcacgt tacgctgatg acataatcat tttagtaaaa acgaaaagcg aaggattaat
     1981 aatccagcgg gaaattactg cgttcatcac taagcgatta aagctcaaag tgaacgaatc
     2041 gaaaagtcga gtgggtccag tatcaggctc aaaatttctc gggttcacat tccgttacgg
     2101 tcaagtgcag atccacgaac aggcgctaaa gaaattcaaa gcaaatgtca gggaactcac
     2161 taaccgcaac tgggggatct caatgaccct acaaattcat aaactcacgc agtatttgcg
     2221 cggctgggga cactactacc tgatagccaa cgcttatcag ctaacggtgg atttagatca
     2281 ttggatccga cgtaggataa gaatgtgcta ctggcggcaa tggcgtaaac ctcgcaccaa
     2341 agtacgcagt ttaatgaaat tgggtgttag cgaacgactc gccatagcct gtggtatcac
     2401 cagtaaaggc ccttgtagaa gctcaaaaac aaaaggtatt aacattgcgc taggcaataa
     2461 ctacttagct tcacaaggtt tagtgtcatt gagggatatt tggatcaata ttcattacgg
     2521 gagatgaacc gcccattgcg gacccgcatg atgggtggtg tgggggctgg aggttagaga
     2581 cctccggcta cccgattatg caaaggacta gtcttcaatg acgtgtaaac cacggcgctt
     2641 taagtcctct aacgaatcca acattcccct tattaattca acaggatgcc cctcccagtc
     2701 ttcaacaacg ccaacaattc tcaagggttc gcaggttcta taggactgtg ttggattacc
     2761 gggaaatttt ttgttcgtaa gattcggatc gtcttcgaac ggtcctgttg gctcaactat
     2821 gtatatgtag ccgcgaccct cgaggccaga cagtgacata gcaagttcag ctccccaaac
     2881 tgctggctcc atcaaggctg aaaagtagat gtgcttaaga a
//
