LOCUS       NZ_CP084049             3632 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P5, complete sequence.
ACCESSION   NZ_CP084049
VERSION     NZ_CP084049.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084049.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P5"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(2116..3632,1..>1))
                     /locus_tag="KWJ12_RS27015"
                     /old_locus_tag="KWJ12_27015"
                     /pseudo=""
     CDS             complement(join(2116..3632,1..>1))
                     /locus_tag="KWJ12_RS27015"
                     /old_locus_tag="KWJ12_27015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /name="Tn3-like element TnAs1 family transposase"
     gene            812..1141
                     /locus_tag="KWJ12_RS27020"
                     /old_locus_tag="KWJ12_27020"
     CDS             812..1141
                     /locus_tag="KWJ12_RS27020"
                     /old_locus_tag="KWJ12_27020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_000493286.1"
                     /translation="MFSVVYHPEAREEATALPVKIRVKFDRLIGKLEYDARLLREPDTK
                     PLGDGLFEIRTMGTDIARGIWVYHKGNTIIMLRVFIKKSQKTPAKEIDLAKKRLAEVLN
                     ETGKP"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            1122..1403
                     /locus_tag="KWJ12_RS27025"
                     /old_locus_tag="KWJ12_27025"
     CDS             1122..1403
                     /locus_tag="KWJ12_RS27025"
                     /old_locus_tag="KWJ12_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="WP_000780222.1"
                     /translation="MKLVSHKELHNEWMQDDEYRAAWQEEERKEKLRELLAEWRKHDNL
                     TKAQVAERMGVTPPVISRLENNITKASIDTLTRYAHACGIKKPVIALY"
                     /name="helix-turn-helix transcriptional regulator"
     gene            complement(1550..2026)
                     /locus_tag="KWJ12_RS27030"
                     /old_locus_tag="KWJ12_27030"
     CDS             complement(1550..2026)
                     /locus_tag="KWJ12_RS27030"
                     /old_locus_tag="KWJ12_27030"
                     /inference="COORDINATES: protein motif:HMM:NF023227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="WP_194164765.1"
                     /translation="MAITGFAARAGEKLRQEKQHCRAISVFIRTSPFSPRDAPCASQAT
                     ETLLVATQDSRDIIAAAQRALARIWRDGYRYAKAGIMLADFSGREAQLNLFDECAPRPD
                     SEALMNAVDRINRAGKGRIFFAGQGINVGFAMRREMLSPEYTTDWNALPRAFMR"
                     /name="DUF4113 domain-containing protein"
ORIGIN
        1 cacaccatca tgttctgagt ttacgtcttt ttttatctga cggtcggcat tcagaatctc
       61 aggtgatttt gcccagtcag gtatatcact aactttatat gtaaaagtca ctcgggaaat
      121 tttctgcccc attgcatcag atggttcagt gaaattactg acgtctgtta ctgttgcttt
      181 tccaaagcag aatccaccaa gattctcacc tcgcatattt ttagccgcat cttgcttgta
      241 gtattttttc ccttcttccg tcaggtcata actgttaacg actatatctc gttctttaat
      301 cccccatgtg gcatcaatat gtttacgcga taattctgtt tcagttaaca accctttttc
      361 tgccattgca tgcaatattt ctggcatgtt gttaatgttg aaaccatctg ttttgtaagg
      421 gaaattagct ttgatataac atgccgggta tacagtatcc agatactgct gtgcggcttc
      481 tttgaaatta ctttcggagg gtttctttgg atcgttacaa ccggtcagcg ccaacacgcc
      541 aatacataat aacggtgcca gataattcac tttcatataa ttgtatcctt gtctcaaaat
      601 tatcccttcg ttcagtacat agaaaaaatg ttaaggcact gatgtaaaaa ggaacgtaaa
      661 agttaaaaaa tacaatttta gagccttttg tcaatgctgg gtccgtgtca tagctggtcg
      721 catacccctc cctaaaaatt agcttacagg ttaattttca ccttgcctaa cattagccta
      781 taggttaata ttaatggaaa tgggaggtca catgtttagc gttgtttatc acccggaagc
      841 cagggaagaa gccacagcat tacccgtaaa aatcagggtg aagtttgacc gtctcatcgg
      901 caaactggag tatgatgccc gattactgcg ggagccggac acgaagcccc tgggcgatgg
      961 actttttgag atccgcacga tggggacgga catagccagg ggcatctggg tataccacaa
     1021 aggcaatacc atcatcatgc tccgggtttt catcaagaag tcacagaaaa cgccagcgaa
     1081 agaaatcgat ctcgccaaaa agcggttagc ggaggtactt aatgaaactg gtaagccata
     1141 aagaactgca taacgagtgg atgcaggacg acgaatatcg cgctgcatgg caggaagaag
     1201 aacgcaagga gaagctcaga gagctacttg cggaatggcg taaacatgac aacctgacaa
     1261 aagcgcaggt tgctgaacgc atgggggtta cacctccggt tatttcccgc ctggaaaaca
     1321 acatcaccaa agccagcatc gacactctga cccgctacgc tcacgcctgc ggcatcaaaa
     1381 aacctgtcat tgccctgtac tgatgacatc ctacccggtt ttagttttcg ccgggtaggt
     1441 gttgtctcaa caggaaaacg cggtacagga ggtctgaagc ccaatgggga agaaccatac
     1501 gttaataatg taaccatatg attttacatc tataaaatcc agatatcgct tatctcatga
     1561 aagcacgcgg caaggcgttc cagtctgtcg tgtattccgg actcagcatt tccctgcgca
     1621 tcgcgaaccc cacattgatc ccctggccgg caaaaaaaat gcggccctta ccggcgcggt
     1681 taatccgatc gacggcattc atcagggctt cgctgtcagg acggggagca cattcatcaa
     1741 acagattaag ttgtgcctcg cggccggaaa aatcggccag catgatacca gcctttgcgt
     1801 accggtagcc atcacgccag atgcgcgcaa gcgcacgctg ggcagcggca atgatgtccc
     1861 ggctgtcctg agtggctacc agtagcgtct ccgtcgcctg gctggcacag ggcgcatccc
     1921 ggggtgaaaa cgggctggtt cggataaaga cactgatcgc acgacagtgc tgcttttcct
     1981 gacgcagctt ttctcctgcc cgcgcagcaa agccggtgat ggccatctgc atttcctgaa
     2041 agctggttac acgctcacca aatgacctgg agacgacaat ttggggagcc cgcagaattc
     2101 ggaaaaaatc gtacgctaag gttttccggg catccgtaag ggccgaaact tcccgtcttc
     2161 cagtctgcgg ctctgccgcc agacgtaatc gccggttagg ttgatgtgct cccagcccag
     2221 cggcgacagg aattgcagca gctcgccgtc caccggcttg ccggcctcga ccaacccctg
     2281 ggtggcgcgt tccaggtaca ccgtgttcca cagcacgata gccgccgtca ccaggttgag
     2341 gccgctggcc cggtagcgct gctgctcgaa gctccgatcc ctgatttccc caaggcggtt
     2401 gaagaacacc gccctggcca gcgagttgcg cgcctcacct ttgttcaggc cggcatgcac
     2461 gcggcggcgc agttcaacac tttgcagcca gtccaggatg aacagcgtgc gctcgatccg
     2521 gcccagctcg cgcagggcca cggccagtcc gttctggcgc gggtagctgc cgagcttgcg
     2581 cagcatcagc gaggcggtga cggtgccctg cttgatcgag ctggccaggc gcaggatgtc
     2641 gtcccagtgg gcacgcacgt gcttgatgtt cagggtgccg ccgatcagcg ggcgcaacgt
     2701 cgggtaggct tgcacgccct gcggcacgta cagcttggtt tcgccgaggt cgcggatgcg
     2761 cggcgcgaag cggaagccta gcaggtgcat cagggcaaag acgtgatcgg tgaagccggc
     2821 cgtgtcggtg tagtgctcct cgatccgcag gtcggactcg tggtacagca ggccgtcgag
     2881 cacataggtg gaatcgcgga cgccgacatt caccacgcgg gtgctgaacg gcgcgtactg
     2941 gtcggagata tgggtataga acagccgtcc cggctcgcta ccgtacttcg ggttgacgtg
     3001 cccggtgctc tcgccccggc cacccgcgcg gaagcgctgg ccatcggagg atgaggtcgt
     3061 gccgtcgccc cagtgggcgg caaaggcgtg gcgatactgg tggttgacca gctcggccaa
     3121 ggccgccgaa taggtttcgt cgcggatgtg ccaggcttgc agccaggaca gcttggcgta
     3181 ggtcaggccg gggctcgact cggccatctt ggtcagcccg aggttgatcg catcaccgag
     3241 gattgcggac agcagcaacg tcctgtcttt ggcctcggcc ccgtccttca agtgggtgaa
     3301 gtggcggctg aagcccgtcc agtcgtccac gtccatcagc agttcggtga tcttgatgcg
     3361 cggcagtaac tggctggttt ggtcgatcag cgcctgcgcc cgatccggca ccgccgcatc
     3421 cagcggggtg attttcagcc ctgactcggt gaggatggca tcgggcagct cgttgtcctt
     3481 ggccaggcgg gtgacggtgg ccaactgctc gtccagcagc tgcaaacgct cttccaggta
     3541 ctggtcgctg ttcgggttga tcgccagggg cagggcctgc tcgcgcttga gtgcggcgaa
     3601 cttctcggcc ggcagcaggt agtcgtcgaa gt
//
