LOCUS       NZ_CP084048             3998 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P4, complete sequence.
ACCESSION   NZ_CP084048
VERSION     NZ_CP084048.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 3998)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3998)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084048.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..3998
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P4"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            complement(join(3866..3998,1..1061))
                     /gene="mobQ"
                     /locus_tag="KWJ12_RS26990"
                     /old_locus_tag="KWJ12_26990"
     CDS             complement(join(3866..3998,1..1061))
                     /gene="mobQ"
                     /locus_tag="KWJ12_RS26990"
                     /old_locus_tag="KWJ12_26990"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MobQ family relaxase"
                     /protein_id="WP_076997031.1"
                     /translation="MATFHISFKKLRRSEGKSSVYLSAYQNREKTKDNRTGATWDYSKK
                     EGFFGSAILSPAGTPAELVKDSGTLWNAVEAGEKRKDAELCRYLDIAIPKELDDGQKKQ
                     IVLDYCQENFVDYGMIADIAFHDLDSHNPHAHVMLTLRTVSPNGFGNKQREWNNRENIE
                     AWRVNWEIIANDRLKKYGHKSRIDSRSLKDQKEEAERKAVFAKTPKEKAKWISKTIELD
                     RTPMTRIHRHQWRKGQKLRAMEQKEKALLLKIANATYLQQTKPPQPEETMKIEKKPLAR
                     SIIDNLKALYKKTQHIFKSKPNVNKNKKEEKEDPQNFVSSYVINEFGEHILKTELETKP
                     KTGTGTGTGGNNGGGKMGSGSGMSPMNQPQPKIEEPEPEPMPEFEDQQPKRKKKFGM"
                     /name="mobQ"
     gene            1305..1688
                     /locus_tag="KWJ12_RS26995"
                     /old_locus_tag="KWJ12_26995"
     CDS             1305..1688
                     /locus_tag="KWJ12_RS26995"
                     /old_locus_tag="KWJ12_26995"
                     /inference="COORDINATES: ab initio prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_071579763.1"
                     /translation="MNKLITEAEGAKIESVFKYILEASESILIEHGHVITGQIIKNKKF
                     FADNGHKDFERLEYYLSSAISSLVEDKVTEGIKDINTDENLVFKKINELSDKEKLILAE
                     CIIKHFEYSNVRGMQKAFNIFGG"
                     /name="hypothetical protein"
     gene            1692..1976
                     /locus_tag="KWJ12_RS27000"
                     /old_locus_tag="KWJ12_27000"
     CDS             1692..1976
                     /locus_tag="KWJ12_RS27000"
                     /old_locus_tag="KWJ12_27000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
                     /protein_id="WP_071579762.1"
                     /translation="MSKTTAQQIADLQNKLQALRTKKARQDRREETRQKIILGAEVAKV
                     LGKKPEEINKALILGLLSKINELDEEEKQEFTRLGLNILEEWKNENRKP"
                     /name="conjugal transfer protein TraD"
     gene            complement(2215..2478)
                     /locus_tag="KWJ12_RS27005"
                     /old_locus_tag="KWJ12_27005"
     CDS             complement(2215..2478)
                     /locus_tag="KWJ12_RS27005"
                     /old_locus_tag="KWJ12_27005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_045287009.1"
                     /translation="MIINGKKIKAKEIMEMTGRSERTVRKYFSQSRDDYEKTAMDRRRQ
                     AYELRSQGLKWQQVADKMGCSYHGAVALYRRYVALDMPQNSL"
                     /name="hypothetical protein"
     gene            complement(2475..3329)
                     /locus_tag="KWJ12_RS27010"
                     /old_locus_tag="KWJ12_27010"
     CDS             complement(2475..3329)
                     /locus_tag="KWJ12_RS27010"
                     /old_locus_tag="KWJ12_27010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="WP_071579761.1"
                     /translation="MIIQEEHNIDFFKNQMPNKPYCTNNLDMGLSIRNKAKALEMLYLQ
                     ANQPAIQTCLLFDLDKKNSFYTFEQVGLPIPHFITKTPKTGRCHYGYMLKAGVCKTQQA
                     RLKPLKYAAAVEMAMAKKLKADLGFAGLITKNPLNDHWSPFWSGADLYDLDYLADFVDL
                     EKPQIQKKKSEAYGLGRNVNLFEDLRQYAYRTVLNFKKISTFEKFENELLLKAQGLNTF
                     CNPQNPLQYKEIKATVRSVARWTWKNFDSHTFSQIQSNRAKKPRKTNVKNEMLDILMSK
                     GVL"
                     /name="replication initiation protein"
     Typing          complement(52..1061)
                     /name="MOBQ"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="100.0"
                     /coverage="85.0"
                     /evalue="0.0"
ORIGIN
        1 cctccattat ttccaccggt tccggttcct gtgcctgttt taggttttgt ttctaactct
       61 gtctttaata tatgttcgcc aaattcattt attacataac tactaacgaa attttgagga
      121 tcttcttttt cttctttttt atttttattt acattaggtt ttgatttaaa tatatgctgg
      181 gtctttttgt ataatgcctt taaattatca atgattgacc ttgcaagagg ctttttctct
      241 attttcatag tttcctctgg ctgaggtggt ttggtttgct gtaagtatgt tgcattagct
      301 atttttaata ataatgcttt ttctttttgc tccatagctc ttaatttttg accttttcgc
      361 cattggtgcc tgtgtatcct ggtcattggc gttctgtcca gttcaatagt ttttgaaatc
      421 cacttggctt tctctttcgg agttttagca aaaacagcct ttctttctgc ctcttctttt
      481 tggtctttta atgaacggga gtcaatacga gatttatgac catatttttt taatcggtca
      541 tttgctatta tttcccagtt aactcgccag gcttcaatgt tttctctatt attccattct
      601 ctttgtttgt tcccaaatcc atttgggcta acagttctta aagttagcat tacatgagca
      661 tgagggttat gactatcaag atcatgaaat gctatgtctg caatcattcc ataatccacg
      721 aaattttctt ggcagtaatc gaggacgatc tgcttcttct ggccgtcgtc cagctccttg
      781 gggatggcta tgtccagata acggcataat tcggcatcct ttcgcttctc gccagcctct
      841 acggcattcc agagggtgcc tgagtccttc accagttcgg caggtgtgcc ggcaggggag
      901 aggatagcag agccgaaaaa tccctctttt tttgaataat cccaggttgc cccggttcga
      961 ttatcttttg tcttttctcg gttttgatat gcagataaat aaaccgaaga tttaccttct
     1021 gatcttcgta attttttaaa gctgatatga aatgtcgcca tagtctaccc cttacttttt
     1081 ttgtcttttg aattttggtt tttagccccg cagggcccat acgtcgcaag cgacgtgtaa
     1141 gtgggcatta ctaatattat tttttcggtt gcacctcaaa ataataagta atgaacccca
     1201 ggcttcgccc aatattaaat attatttgtt ttattctaaa atatatttga ctaaagtaaa
     1261 taatggaggt aataaataac aaaataaata attaggaggt tattatgaat aaacttataa
     1321 cagaggcaga aggtgcaaaa atcgaaagtg tttttaaata catattagag gcatctgaaa
     1381 gcatattaat agagcatggg catgttatta cggggcaaat aataaaaaac aaaaagttct
     1441 ttgctgataa cgggcataaa gattttgaaa ggttagagta ttaccttagc tcggcaatta
     1501 gttctcttgt cgaggataag gtaacagaag gcattaaaga cattaacacg gatgagaacc
     1561 ttgtttttaa aaaaataaat gaattgagtg acaaggaaaa attaatttta gcagagtgca
     1621 ttattaaaca ttttgaatat tcaaatgtta gaggaatgca aaaggcattt aatatttttg
     1681 gggggtaatg gatgagtaaa acgacagcac agcagatcgc agatttgcaa aataaactac
     1741 aagcattaag aacaaaaaaa gcaagacagg acagaaggga agaaacaaga caaaaaataa
     1801 tcctgggtgc agaagtggct aaggttttag gtaaaaaacc ggaggaaatt aacaaggctt
     1861 taattcttgg gttgttaagt aaaataaatg aacttgacga agaagaaaaa caagaattca
     1921 cacgattagg attaaatatt ttagaagagt ggaagaatga aaatcgtaaa ccgtgaacaa
     1981 taaaaaacca gccgatcttg gctggttttt tactgtgaag ggttgccagg cttgcctgga
     2041 ggtcttaaaa aggctcagag aggctctcag ggcttatggg tgggtttgtt gattgttggg
     2101 tacctgagtg cctctcagtt cgagatacag ggcccggata cggggaaagg ggaaaaaatc
     2161 cccccagggg tggagggttt ggttaggtta gggggaaaaa ctgcttagaa gtcgttacag
     2221 gctgttttga ggcatatcta aggcaacata acggcgatac agggcaacgg ctccgtgata
     2281 cgaacagccc attttgtcgg ctacttgttg ccatttcagc ccctggcttc gtagttcgta
     2341 agcctgacga cgacgatcca ttgctgtttt ttcgtaatcg tcacgagatt gagaaaaata
     2401 ttttcttaca gttctttcac tacgacctgt catttccatt atttcttttg cttttatttt
     2461 tttaccatta atgatcataa aacccccttg ctcattaata tgtctaacat ttcattttta
     2521 acattggttt ttcgaggttt tttggctctg tttgattgga tttgtgaaaa tgtatggcta
     2581 tcaaaatttt tccaagtcca acgggctaca cttctaactg ttgcttttat ctctttgtat
     2641 tgtaacgggt tttgtggatt gcaaaaagtg tttaagccct gtgcctttaa tagtagttcg
     2701 ttttcgaatt tctcgaaagt ggatattttt ttgaaattta aaaccgttct gtaagcatat
     2761 tgccgtagat cttcaaacaa attaacattg cgacctaaac cataagcctc gctttttttc
     2821 ttttgtattt gaggcttttc taagtccaca aaatcagcta aataatccag gtcataaagg
     2881 tcagcacctg accagaacgg cgaccagtga tcattgagtg ggttttttgt aatcaatcca
     2941 gcaaacccca gatctgcttt tagttttttc gccatagcca tttcaacggc tgcggcatac
     3001 ttcaacggct ttaaccttgc ttgttgtgtc ttacaaaccc cggctttcag catatagccg
     3061 tagtgacaac gacctgtttt tggtgttttt gtaataaagt gaggtatggg taagcccacc
     3121 tgctcaaatg tatagaatga atttttctta tctagatcaa agagtaagca agtttgaatg
     3181 gctggctgat ttgcctgtaa atacagcatt tcaagggctt ttgccttgtt ccttatgctt
     3241 aatcccatat cgaggttatt tgtacaataa ggcttgttag gcatttgatt tttaaaaaaa
     3301 tcgatgttat gttcttcttg tattatcatt ttttcagtcc tttttatctg gccctactct
     3361 ctgttttctg tcgccaaaac aaaatcgcag agggtggggt ttttattttt atagattttt
     3421 caatcctgta ccagttctaa ccgatctcaa aaatttgtca agtgtgcgtc taaccatatc
     3481 agataacagc ccaacgaagt tggggcagtt ctttacatac ggcagcaaag cattactatc
     3541 agatctatgg tgcgaagcat taccgaagat agaaaaacaa taaaaaaaga aaaaagagta
     3601 ttgttcgaag aaaaattggc gattcgattt tttttggtga atatatgaaa aaaaagaaag
     3661 tgcagaaaaa acggaaaaga aataagcata gtaaattttt taaaatgctt atgaagtcag
     3721 ttaatgaagc tgtggcaata cataaagggg aaatagaacc ggcgaggatc acaactctaa
     3781 aaatgtagta tcttatagta tagtattaaa taatattaag ttgtattaag tgatactact
     3841 atataggaat atataccact atatactaca ttccaaattt cttcttcctc ttaggttgct
     3901 gatcttcgaa ttccggcatt ggttccggct caggctcttc aatttttggt tgtggttggt
     3961 tcataggaga cattccagag cctgaaccca tttttccg
//
