LOCUS       NZ_CP084046             8380 bp    DNA     circular CON 10-JAN-2024
DEFINITION  Klebsiella pneumoniae subsp. pneumoniae strain WRC12_S471M plasmid
            pWRC12_P2, complete sequence.
ACCESSION   NZ_CP084046
VERSION     NZ_CP084046.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN20114092
            Assembly: GCF_020215625.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae subsp. pneumoniae
  ORGANISM  Klebsiella pneumoniae subsp. pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 8380)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Carbapenem resistant Klebsiella pneumoniae_WRC12_S471M
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8380)
  AUTHORS   Balaji,V., Snehal,P., Yamuna,B.D. and Monisha,P.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil Nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: UNICYCLER v. V.0.4.8
            Assembly Name                     :: WRC12_S471M
            Genome Representation             :: Full
            Expected Final Version            :: Yes
            Genome Coverage                   :: 126.43x
            Sequencing Technology             :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/10/2024 04:06:51
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,426
            CDSs (total)                      :: 5,304
            Genes (coding)                    :: 5,149
            CDSs (with protein)               :: 5,149
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 84
            ncRNAs                            :: 13
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 66 of 155
            Pseudo Genes (incomplete)         :: 90 of 155
            Pseudo Genes (internal stop)      :: 20 of 155
            Pseudo Genes (multiple problems)  :: 19 of 155
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP084046.1.
            Bacteria and source DNA available from Christian medical college.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..8380
                     /organism="Klebsiella pneumoniae subsp. pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="WRC12_S471M"
                     /isolation_source="urine"
                     /host="Homo sapiens"
                     /sub_species="pneumoniae"
                     /db_xref="taxon:72407"
                     /plasmid="pWRC12_P2"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2014"
                     /collected_by="Christian medical college"
     gene            join(8247..8380,1..>1)
                     /locus_tag="KWJ12_RS26895"
                     /old_locus_tag="KWJ12_26895"
                     /pseudo=""
     CDS             join(8247..8380,1..>1)
                     /locus_tag="KWJ12_RS26895"
                     /old_locus_tag="KWJ12_26895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /name="DUF4158 domain-containing protein"
     gene            74..934
                     /locus_tag="KWJ12_RS26900"
                     /old_locus_tag="KWJ12_26900"
     CDS             74..934
                     /locus_tag="KWJ12_RS26900"
                     /old_locus_tag="KWJ12_26900"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF000285.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extended-spectrum class A beta-lactamase SHV-12"
                     /protein_id="WP_002904004.1"
                     /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKQSESQLSGRVGMIEM
                     DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV
                     SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWET
                     ELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLP
                     AGWFIADKTGASKRGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALI
                     EHWQR"
                     /name="extended-spectrum class A beta-lactamase SHV-12"
     gene            complement(955..1716)
                     /locus_tag="KWJ12_RS26905"
                     /old_locus_tag="KWJ12_26905"
     CDS             complement(955..1716)
                     /locus_tag="KWJ12_RS26905"
                     /old_locus_tag="KWJ12_26905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226821.1"
                     /GO_function="GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="WP_002210513.1"
                     /translation="MIPIERHQRILALVEQRGAVSINELTEILGVSHMTIRRDVSKLEE
                     QGLLVSVSGGVRAVSRLAAEPSHLVKSTLQSEEKQAIGALAASHIAKNSCIYLDAGTTT
                     LALARAILDRNDLQVVTNDFEITQLLIDASQCGVIHTGGTLCRENRSCVGESAARTLRH
                     LAIDTAFISASGWDSRGIFTPDENKVTVKETVSQVSARSILLCDSSKYNQVATFMALPL
                     TRFTTIITDRHLSDAAASHIARHACEVLRAG"
                     /name="DeoR/GlpR family DNA-binding transcription
                     regulator"
     gene            1978..2880
                     /gene="ltnD"
                     /locus_tag="KWJ12_RS26910"
                     /old_locus_tag="KWJ12_26910"
     CDS             1978..2880
                     /gene="ltnD"
                     /locus_tag="KWJ12_RS26910"
                     /old_locus_tag="KWJ12_26910"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226820.1"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="WP_002903955.1"
                     /translation="MAAHTNVCVIGLGSMGMGAARACLQAGLNTWGVDINPDNCRALLA
                     AGAKGAGPSAVPFAAELDAVVLLVVNAAQVRGILFGESGLAAHLKPGTVVMVSSTIASA
                     DAQAIAEALAEYQLLMLDAPVSGGAVKAAAGDMTVMASGSDAAFARLAPVLDAVAGKVY
                     RIGSDIGLGSTVKIIHQLLAGVHIAVAAEAMALAARAGIPLETMYDVVTHAAGNSWMFE
                     NRMQHVLDGDYSPKSAVDIFVKDLGLVNDTARALTFPLPLATTALNMFTSASNAGFGRE
                     DDSAVIKIFNGITLPGHKQ"
                     /name="ltnD"
     gene            2892..4144
                     /gene="otnK"
                     /locus_tag="KWJ12_RS26915"
                     /old_locus_tag="KWJ12_26915"
                     /pseudo=""
     CDS             2892..4144
                     /gene="otnK"
                     /locus_tag="KWJ12_RS26915"
                     /old_locus_tag="KWJ12_26915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226819.1"
                     /GO_function="GO:0005524 - ATP binding [Evidence IEA];
                     GO:0016301 - kinase activity [Evidence IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /name="otnK"
     gene            4137..>4430
                     /locus_tag="KWJ12_RS26920"
                     /old_locus_tag="KWJ12_26920"
                     /pseudo=""
     CDS             4137..>4430
                     /locus_tag="KWJ12_RS26920"
                     /old_locus_tag="KWJ12_26920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /name="class II aldolase/adducin family protein"
     gene            4573..>6114
                     /locus_tag="KWJ12_RS26925"
                     /old_locus_tag="KWJ12_26925"
                     /pseudo=""
     CDS             4573..>6114
                     /locus_tag="KWJ12_RS26925"
                     /old_locus_tag="KWJ12_26925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032490160.1"
                     /GO_function="GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006313 - DNA transposition [Evidence IEA]"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo=""
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element IS3000 family transposase"
                     /name="Tn3-like element IS3000 family transposase"
     gene            complement(6157..7077)
                     /locus_tag="KWJ12_RS26930"
                     /old_locus_tag="KWJ12_26930"
     CDS             complement(6157..7077)
                     /locus_tag="KWJ12_RS26930"
                     /old_locus_tag="KWJ12_26930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150544.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_022631165.1"
                     /translation="MKIGYARVSTKDQTVDLQLDALKQAGCERIYQEVASGAKTARPEL
                     DRLLVDIRAGDTIVIWKLDRLGRSLKHLVELVGKLAERKIGLLSLNDPVDTTNAQGRLV
                     FNLFASLAEFERELIQERTLAGLSAARARGRVGGRPKGLSAPAESTAMAAETLYREGRL
                     SVSAIGKKLHISKGTLYRYLRSRGVEIGKQKKNQLTDTLQPTAINGSPITKTATVSLLF
                     SVENNSKFVRGKKRAQANIEQYSLALYDNKQLAPGKYELRIPYENEHELNETMHQLLSD
                     MNREADLCNCNVDANAWEEGTERRW"
                     /name="recombinase family protein"
     gene            complement(7190..7765)
                     /locus_tag="KWJ12_RS26935"
                     /old_locus_tag="KWJ12_26935"
     CDS             complement(7190..7765)
                     /locus_tag="KWJ12_RS26935"
                     /old_locus_tag="KWJ12_26935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003159185.1"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA];
                     GO:0000150 - DNA strand exchange activity [Evidence IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="WP_001173919.1"
                     /translation="MALIGYARVSTAEQDTALQTDALRNAGCERVFEDTASGAKADRPG
                     LADALAYLRDGDVLVVWRLDRLGRSLPHLIETVGKLEARGVGFRSLTENIDTTTPGGRL
                     IFHVFGALGQFERDLIRERTKAGLTAAAARGRKGGRKPVVTADKLQRARELVANGLNVR
                     EAAARLKVGKTALYAALQAASSGSAADS"
                     /name="recombinase family protein"
     gene            complement(7892..8140)
                     /locus_tag="KWJ12_RS26940"
                     /old_locus_tag="KWJ12_26940"
     CDS             complement(7892..8140)
                     /locus_tag="KWJ12_RS26940"
                     /old_locus_tag="KWJ12_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012585400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family toxin"
                     /protein_id="WP_012585400.1"
                     /translation="MQSSIERTVSGVRCTNDDLKPVLVALATDQPLDVRYRDHDLSGDW
                     AGYRECHIKPDLLLIYRKSDADTLRLARLGSHSELFG"
                     /name="type II toxin-antitoxin system YafQ family toxin"
     ARG             75..931
                     /name="blaSHV-12"
                     /gene="blaSHV-12"
                     /gene_name="extended-spectrum class A beta-lactamase
                     SHV-12"
                     /tool="amrfinderplus"
                     /drug_class="BETA-LACTAM"
                     /identity="100.0"
                     /coverage="100.0"
ORIGIN
        1 aacgccgggt tattcttatt tgtcgcttct ttactcgcct ttatcggcct tcactcaagg
       61 atgtattgtg gttatgcgtt atattcgcct gtgtattatc tccctgttag ccaccctgcc
      121 gctggcggta cacgccagcc cgcagccgct tgagcaaatt aaacaaagcg aaagccagct
      181 gtcgggccgc gtaggcatga tagaaatgga tctggccagc ggccgcacgc tgaccgcctg
      241 gcgcgccgat gaacgctttc ccatgatgag cacctttaaa gtagtgctct gcggcgcagt
      301 gctggcgcgg gtggatgccg gtgacgaaca gctggagcga aagatccact atcgccagca
      361 ggatctggtg gactactcgc cggtcagcga aaaacacctt gccgacggca tgacggtcgg
      421 cgaactctgc gccgccgcca ttaccatgag cgataacagc gccgccaatc tgctgctggc
      481 caccgtcggc ggccccgcag gattgactgc ctttttgcgc cagatcggcg acaacgtcac
      541 ccgccttgac cgctgggaaa cggaactgaa tgaggcgctt cccggcgacg cccgcgacac
      601 cactaccccg gccagcatgg ccgcgaccct gcgcaagctg ctgaccagcc agcgtctgag
      661 cgcccgttcg caacggcagc tgctgcagtg gatggtggac gatcgggtcg ccggaccgtt
      721 gatccgctcc gtgctgccgg cgggctggtt tatcgccgat aagaccggag ctagcaagcg
      781 gggtgcgcgc gggattgtcg ccctgcttgg cccgaataac aaagcagagc gcattgtggt
      841 gatttatctg cgggatacgc cggcgagcat ggccgagcga aatcagcaaa tcgccgggat
      901 cggcgcggcg ctgatcgagc actggcaacg ctaagccggc ggtggccgcg cgcgttatcc
      961 ggcccgcagc acctcgcagg cgtgccgggc gatatgactg gcggcggcat cggaaagatg
     1021 ccggtcggta atgatggtgg tgaaccgggt caaaggtaac gccataaacg tggccacctg
     1081 attgtatttc gaactgtcgc acagcaggat gcttctcgcg ctgacctggc tgacggtctc
     1141 cttgacggta accttgttct catcaggggt gaatatcccg cgactgtccc agccgctggc
     1201 ggagataaag gccgtatcga tagccaggtg gcgtaacgta cgcgccgccg attcgcccac
     1261 gcaggagcgg ttctcccggc acagagtgcc gccggtgtgg atcacgccgc actggctggc
     1321 atcgatcagc agctgggtta tctcaaaatc attggtgacc acctggagat cgttccggtc
     1381 gaggatcgcc cgcgccagcg ccagggtggt agtcccggca tccagataga tgcaactgtt
     1441 tttagcgata tgactcgccg ccagcgcgcc gatcgcctgt ttctcctcac tctgcagcgt
     1501 gcttttcacc agatgactgg gttccgcggc cagccggctg acggcgcgta cgccgcccga
     1561 gacgctgacc agcagcccct gctcctccag tttactgacg tcccgacgga tggtcatatg
     1621 ggacacgccg aggatctccg tcagctcgtt aatgcttacc gccccacgct gctccaccag
     1681 ggctaaaata cgctgatgtc gttctattgg aatcaccgct ctccccttac cattttttca
     1741 caccaggcgt caccacccag gctacggccc tggcgacacc cggtgttgtt atcgcgcttt
     1801 gccgctgttt tttacgctat tttagggcaa gaatcgccgc tgtgcagcct ctttccgcct
     1861 gtgaattttt tatattcatg tgggttattc gtgataactc tcacataaat tcacatgata
     1921 acgctttatt ctatcattaa atcacattaa ttaactaatg ttcacaagga gaccagcatg
     1981 gctgcacaca ctaacgtctg cgtgattgga ctgggttcaa tgggcatggg cgccgcccgc
     2041 gcctgcctgc aggcgggcct gaacacctgg ggcgttgaca tcaatcccga caactgtcgc
     2101 gcactgctgg cggcgggcgc caaaggcgcg ggccccagcg cggtgccgtt cgccgcggaa
     2161 ctggatgcag ttgtgctgct ggtggtcaat gccgcccagg tgcgggggat cctgttcggc
     2221 gagagcggcc tcgccgccca tctgaagccg ggcaccgtcg tgatggtgtc gtccaccatc
     2281 gcctccgccg atgctcaggc cattgccgag gcgctggcgg agtaccagct attgatgctc
     2341 gacgcgccgg tatcgggcgg cgccgtgaaa gcggccgccg gcgacatgac ggtgatggcc
     2401 tccgggagcg atgccgcctt tgcccgactc gcgccggtgc tggacgccgt ggccggcaaa
     2461 gtctaccgca tcgggagcga cattggtctt ggctcgacgg taaaaattat ccatcagctg
     2521 ctggccgggg tgcacatcgc cgttgccgcc gaagcgatgg cgcttgccgc ccgcgccggg
     2581 atcccactgg agacgatgta tgacgtggtc acccacgcgg cgggtaattc ctggatgttt
     2641 gagaatcgca tgcagcacgt cctggatggc gattactcgc caaaatccgc tgtcgatatt
     2701 tttgtcaaag atctcgggct ggtgaatgac actgcccggg cgctgacctt cccgctgccg
     2761 ctcgctacca ccgcgctgaa tatgttcacc tccgccagta atgccggatt cggtcgggaa
     2821 gatgacagcg cggtgatcaa gattttcaac ggcatcaccc tgccgggcca taaacagtga
     2881 ggagagacaa catgcagctt ggtgtcattg ccgatgactt caccggcgcc acggatattg
     2941 ccagcttcct cgtgcgcaac ggcatgccga cggtgcaact gaatggcgtg ccgacccgcg
     3001 atattccgct gaccagcgag gcggtggtca tcagcctgaa aacccgctcc tgcccggcgg
     3061 aaatggccgt cagccagtcg ctggcggccc tgcgctggct gcaggcccag ggctgtcagc
     3121 agttttattt caagtactgt tccactttcg acagcaccgc gcagggcaac attggcccgg
     3181 tgctggatgc cctgctggcc gagctgggtg agacgcggac ggtgatttcc ccggcgctgc
     3241 cggttaacgg ccgcacggtc tatcagggat atctgttcgt cggcgagcaa ctgctgaatg
     3301 agtccgggat gcgccaccat ccggtgacgc cgatggagga tgcgcacctg ggccgcttaa
     3361 ttgagcgcca agggcgcgga aaagccgcgc tgattgcctg gccgattgtc gcccggtcgc
     3421 cgccgcgctg gcggcagtca acgatccggc ggtgcgctat gtggtgctcg acgccctcag
     3481 cgaacaggat ctgctcaccc agggcgtggc gctgcgggag atgaagctgg tctccggcgg
     3541 ttccggcctc gccatcggcc tcgcccgcga ctgggcgcag cgccatggcg ccagggggga
     3601 aagcgctcag gccggcatgc cgctggccgg tccggcggtg gtgctctcgg gctcctgctc
     3661 ggtgatgacc aacagccagg tggcggccta tcgtcaacag gcccccgccc gcgccgtcga
     3721 cttaagcgcc tgctttaccg atctggagag ctacgtcagg acgctgactg actgggtgga
     3781 cgcgcagcgc gatgcgccgc tggcgccgat gatctatgcc accaccgagc cgcaaacgct
     3841 gcagcggatc caggcgcagt atggcgacaa ggccagcagc gaacgggtgg aacagctgtt
     3901 tgccgctctt gccgccgccc tgaaggcgaa cggatttacc cgctttattg tggccggagg
     3961 ggaaacgtcg agcattgtgg cgcagaccct gggggttgag gcgttccata ttgggccgac
     4021 catctcccct ggcgtgccct gggtgcgtga cacccgccag ccgctctccc tggcgctgaa
     4081 gtcaggtaac ttcggcgata tccagttctt tgcccgtgcc cagcaggagt ttcgtcatga
     4141 ctgagcaaca actgcgagag gaaatggtgc agattggcgc ctcgttgttt agccgcggct
     4201 atgccaccgg ctccgctggc aatctgtcgc tgctgctgcc ggacggcaac ctgctggcga
     4261 cgccgaccgg cgcctgcctc ggcgaactgc aggctcagcg gttgtcggtg gtgacgctgc
     4321 aaggggaatg gatctccggc gacaaaccgt cgaaagaggt cacttttcac cgggcggtct
     4381 atttgcacaa cccggcctgc aaggcgatcg tccacttgca cagccactat ggggttctag
     4441 ggattttccg tccaaaaacg acaaagtgct ctggaggccc cgccgtctgt ggcctccaga
     4501 ggggtattac ttttttcgag atccctattt gggctctgtt tgagtaagat ttagtctttt
     4561 tgggctaaat ccatggcatt cgaagaacgt gtccaaatct tgtccgaggc agaacaagat
     4621 gagttatatg gaccccccgc tttcacctcc gccgaccaac gcttcttttt ctcgctgaac
     4681 gataaggaac tggcgatcgc taaaagcctc cgtcatcggg gccagcgtta catgctggtg
     4741 gtactgctgg gctatttcaa agccaagccg gtggtgctga atcccggctt tcatcagatc
     4801 aagcaggacc tcaaatacgt ttatcaaacc gttctaccag gcccaggctg tagacccttt
     4861 aatctcacgc caaaagagaa cgaacggatt taccaacgtg ttttccagct ctgcaactac
     4921 cagcgctgga atgtcaaaga ccatggagcg gcgttgagag attacttatc ccagcaagcc
     4981 agagcctgga cagcgcctag gcacctcttc gatgcggcca atgaatattg ttcggggcag
     5041 aagatcgcaa tccctgccta cagcacgctg caaaagatca tcagtcaggt ggtgggagac
     5101 gaacaggaac acatggccgc gcaccttgag cgtgcaatgt cacgcggtct taagcaagca
     5161 ctggcggagc tcgtgaacgg cacaggtcca ctgccgttcc gacagttgcg acaatcggct
     5221 cgtaacttca ccggaaccga gttggagaaa gaactgattg tctatcgcca tatccagcat
     5281 tggatgccgg aagtggatct gctgttgagc acgctatcac tgtcgcagaa aaatctgcaa
     5341 cacctggcag agaaagtcga ctactacggt gccaaactga aacgtcaaac cgtaggcagc
     5401 caatggctgt atttattgtg ctatcttcaa acgcgatggc agcaagcgct ggaacgtatt
     5461 gccgatggct ttgtgcatca tgtcagacag accaaacaga aggcaaagga ttatgcgcag
     5521 gaagcggtgt ttaaggattg gcaaaaagca gctaaaaatg tcagcaaggc agctgaagta
     5581 ctgcacctgt tcattgatga cagcattgat ctgcaactac cgtttgcaac agtaagacag
     5641 caagcactga gcctgctgac caaaagggat ctggaatctg tatgtctctt cctgaacgag
     5701 cagcgacgat cggtcgatga agccatgtgg cagtactgcg acgagaaaga aagtctgcgg
     5761 aaaggtttgc tgcgagagtt gttcctatgt ctgcgcttcg aaggctgcga cggcacccag
     5821 cacttagcgg ccgccttggc gaaaacacaa aacgaactca acggccagga cgctcagttg
     5881 caaactgccg acaccagact cctttccaaa aaatcacgtg aattcctgct ggatggtgaa
     5941 gggaatatcc tgatcgatcg ttatgagtgg ttcctctatc aacagattcc tgatcgcctg
     6001 aatggccagc tgacgctgcc tgatatcact aaataccggg cactcgacgc cgacctgatc
     6061 gacggggaac attggcgaaa aaacaaatat acgctgcttc aacagagcca ttttaggggt
     6121 ttggggagca atggaaccaa aaaccaacgt aagagtctac catcgccgtt cagtcccttc
     6181 ttcccatgca tttgcatcaa cattgcaatt acataaatca gcttctcggt tcatatctga
     6241 tagaagttga tgcatcgttt cattcaattc atgttcattt tcatagggaa tgcgcaactc
     6301 atattttcct ggcgctaact gtttattgtc atagagagcc aagctgtatt gttcgatgtt
     6361 tgcctgggcg cgcttcttac ctctgacaaa tttgctattg ttctcaacgc tgaacagcaa
     6421 gcttactgtg gctgttttgg tgatcggaga tccgtttatt gctgtgggtt gtaacgtatc
     6481 cgttaactgg ttctttttct gcttaccaat ttcaacacct cgtgaccgta aataacgata
     6541 gagcgtgcct ttggatatat gtagcttttt accaatcgcg ctgacactga gtcgcccttc
     6601 gcgatataac gtttcagccg ccattgcggt actttccgcc ggtgcagata aacctttggg
     6661 ccttccgcct acccgacctc ttgctcgtgc ggcggataac cccgcgagtg tgcgttcttg
     6721 aattaattca cgctcaaact ctgccaagga tgcaaacaga ttaaagacca aacggccttg
     6781 ggcgtttgta gtatcaacag gatcattcag actgagcagg ccaatttttc gttcggccaa
     6841 tttgcccacg agctcaacca ggtgtttaag cgaacggcca agccgatcca gcttccagat
     6901 cacgatcgta tcgccagcac gaatatccac taataaacga tccaattccg gccttgccgt
     6961 ttttgctccg ctggcaacct cttgatagat gcgttcacat cctgcttgtt tgagtgcatc
     7021 aagttgtaga tctacggttt ggtctttggt gctcactcgt gcgtagccaa ttttcatcaa
     7081 taaagttcca tttactcaac tgtattaaga atagttaaac tttgatttta agaaccagta
     7141 ttttatacct tatcgcactg tcgttttcag aaggcgactg cacgaaatat caggagtcgg
     7201 ctgcactgcc agaactggcc gcctgcaagg cagcgtagag agcggttttt cctaccttga
     7261 ggcgtgcggc ggcctcgcgg acattcaagc cgttggcgac aagctcccgc gctcgttgca
     7321 acttgtcggc agtgacaacc ggcttgcgtc cgcctttgcg cccgcgtgcg gcggcagcgg
     7381 tcaacccggc cttggtgcgc tcgcggatca ggtcgcgctc gaactggccc agcgcaccga
     7441 acacatggaa gatgagccgt ccgcccggcg tggtggtgtc gatgttttca gtcaacgagc
     7501 ggaagccgac gccccgtgct tccagcttgc cgaccgtctc aatcaggtgc ggcagggagc
     7561 ggccaagccg atccagccgc cagacgacca gcacatcgcc atcgcgcaga taagccagcg
     7621 catcggccaa gccgggccgg tctgccttgg ccccggatgc cgtgtcctcg aaaacccgtt
     7681 cgcagcctgc attgcgcagc gcatccgtct gcaaggcggt gtcctgttcc gccgtcgata
     7741 cccgcgcata gccgatgagt gccatttccg cccatttgtc cgttataccg tccgcctatc
     7801 ttattgtccg tcatcccgtt gcgcaagatg ttttcggaca ggcgtccggt ttggccgcct
     7861 aatgatcgtt tggcggacaa ggcgactggc atcagccgaa cagctcgcta tgggagccaa
     7921 gccgcgccag tcgcagggtg tcggcgtcgg acttgcggta gatcagcagc aggtcgggct
     7981 tgatgtggca ttcgcggtag cccgcccaat cgccggaaag atcgtggtcg cggtatcgca
     8041 catccagggg ctgatcggtc gccagcgcga ccagaaccgg cttgaggtca tcattcgtgc
     8101 agcggactcc tgatactgtt cgttcaatag acgactgcac aacagccttc aaaccgcgtt
     8161 gtctggtgca gtcgtcttct gaaaatgaca ccggttgcct tggcgggctt gtcacaaacc
     8221 tacgttttca agaagaagga aaccgcatgc cccgccgttc gatcctctcc gccgccgagc
     8281 gcgaaagcct gctggcgttg ccggacacca aggatgagtt gatccgtcac tacacgttca
     8341 gcgaaagcga cctctccatc atccggcagc ggcgcggccc
//
