LOCUS       NZ_CP083934             9730 bp    DNA     circular CON 08-JAN-2024
DEFINITION  Klebsiella pneumoniae strain BA4946 plasmid pColRNAI, complete
            sequence.
ACCESSION   NZ_CP083934
VERSION     NZ_CP083934.1
DBLINK      BioProject: PRJNA224116
            BioSample: SAMN21529401
            Assembly: GCF_026427375.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella pneumoniae
  ORGANISM  Klebsiella pneumoniae
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Teekaraman,M. and Shanmugam,S.
  TITLE     Klebsiella pneumoniae BA4946
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Veeraraghavan,B., Shankar,C., Teekaraman,M. and Shanmugam,S.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-SEP-2021) Clinical Microbiology, Christian Medical
            College, Ida Scudder Road, Vellore, Tamil nadu 632004, India
COMMENT     ##Genome-Assembly-Data-START##
            Assembly Date                     :: 2021
            Assembly Method                   :: Unicycler v. June 2021
            Genome Representation             :: Full
            Expected Final Version            :: No
            Genome Coverage                   :: 60.0x
            Sequencing Technology :: Illumina HiSeq; Oxford Nanopore MinION
            ##Genome-Assembly-Data-END##
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI RefSeq
            Annotation Date                   :: 01/08/2024 05:37:06
            Annotation Pipeline :: NCBI Prokaryotic Genome Annotation Pipeline
            (PGAP)
            Annotation Method :: Best-placed reference protein set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 5,638
            CDSs (total)                      :: 5,516
            Genes (coding)                    :: 5,355
            CDSs (with protein)               :: 5,355
            Genes (RNA)                       :: 122
            rRNAs                             :: 9, 8, 8 (5S, 16S, 23S)
            complete rRNAs                    :: 9, 8, 8 (5S, 16S, 23S)
            tRNAs                             :: 85
            ncRNAs                            :: 12
            Pseudo Genes (total)              :: 161
            CDSs (without protein)            :: 161
            Pseudo Genes (ambiguous residues) :: 0 of 161
            Pseudo Genes (frameshifted)       :: 67 of 161
            Pseudo Genes (incomplete)         :: 92 of 161
            Pseudo Genes (internal stop)      :: 28 of 161
            Pseudo Genes (multiple problems)  :: 23 of 161
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
            REFSEQ INFORMATION: The reference sequence is identical to
            CP083934.1.
            Bacteria and source DNA available from Department of Clinical
            Microbiology, Christian Medical College, Vellore, India.
            The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Klebsiella pneumoniae"
                     /mol_type="genomic DNA"
                     /strain="BA4946"
                     /isolation_source="blood"
                     /host="Homo sapiens"
                     /db_xref="taxon:573"
                     /plasmid="pColRNAI"
                     /geo_loc_name="India"
                     /lat_lon="12.9165 N 79.1325 E"
                     /collection_date="2020"
                     /collected_by="Department of Clinical Microbiology,
                     Christian Medical College, Vellore"
     gene            join(9082..9730,1..437)
                     /locus_tag="LCH45_RS28030"
                     /old_locus_tag="LCH45_28065"
     CDS             join(9082..9730,1..437)
                     /locus_tag="LCH45_RS28030"
                     /old_locus_tag="LCH45_28065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="WP_063938519.1"
                     /translation="MAITIKSAVNVRSRVLIYSLLLFTGSAYAADIAESTFLTRDIGNG
                     LYEMSFDGKNNSLWAASSSSFDRDKGHGIVYQLEPSTLKTKSAVKIARLGFATALDEEH
                     ERLYIGNTLDGSVTVIDTLTGKELSVIPLTEGQDEAHFRPTREMVIDKKNQRLYVSSAA
                     DKGALWVIDMAVGKKIKTIDGLGHAVTGIALNKNGDNIYLVNGDGEIINLDASSYEIKK
                     RFVVEPEKKHFFLNVSLDEKNGRAFITDPDIPGVLVVDVNSGKILHRIDVINSLAILFN
                     PLRNEIYITHRNAKQVSIVDSNSYYVKTTIKTRLMPNSLSLSHDGENLYVSVKQEKKEM
                     STMKDYILKIELSKIAMEPEE"
                     /name="YncE family protein"
     gene            434..2623
                     /locus_tag="LCH45_RS28035"
                     /old_locus_tag="LCH45_28070"
     CDS             434..2623
                     /locus_tag="LCH45_RS28035"
                     /old_locus_tag="LCH45_28070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01783.1"
                     /GO_component="GO:0019867 - outer membrane [Evidence IEA]"
                     /GO_function="GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA]"
                     /GO_process="GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="WP_063938521.1"
                     /translation="MNKYLGLSIVYMMYTSSVMADDSGAVSKDKNISPEETLIVHGTAV
                     SESTTEGTDSYTTQSANTASKMNLSFRDTPQSTTVITRRRMDDVAAKNIIDVVKTAPGL
                     YVSTAEGSGRPNFMSRGFNANVMYEGFSLPWNSYIPSSQTSSAIFDRVEIVRGATGLVQ
                     GAGDPSAAINMIHKRPTRQYQASTNIGAGRWNDYRGGVDVSGPLNDSATLRGRAVVDGE
                     NSETFRDSEKRRNNVIYGIMEADLTDNTLLTIGGYQQYEYNNHWWYDLPISGTGHHLNL
                     PRSTFTGNDWEFERSKSKTAFATLEQKFSNSWKLKFSTLQNWRNADLLGTATYRHKDTR
                     QSMYQQVWGSLREYNSKSYDLSLNGDYALFGRVHQFVAGTTQQKYTDKDTGYTYSPIYI
                     ENVNIFDHGSHSSKKPMKMSGDASYDVTEQKSAYAATHFLLSDNWKILLGGRLDWYEYT
                     SKGTSINTGYKVTRNITKYAGSTYNLDDHHTIYGSYTDIFNPQSSTDLNGKVIKPIVGK
                     NYETGIKGEYFNGLLNASLAVFQVDQENRARLLPDQTGCVTNGNPQCYEASGKVRTKGI
                     DFELQGMVTPLWEIGVGYTYADTRYIKDGDRSNEGKRFNTAVSPQNLFKISSMYTFDGK
                     LTNWRAGVNTYWQSKIYANITDTRDGNPVINKQKAYSTTDIIIGYTPMPQLDLQLNITN
                     VFDKVYYTSIGESIGYGTETYGEPRNVKISATYRF"
                     /name="TonB-dependent siderophore receptor"
     gene            4209..6170
                     /locus_tag="LCH45_RS28040"
                     /old_locus_tag="LCH45_28075"
     CDS             4209..6170
                     /locus_tag="LCH45_RS28040"
                     /old_locus_tag="LCH45_28075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004178196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="WP_063938523.1"
                     /translation="MFNTDSLAWQWHIPELARQIYPWVSWLYREYYGLAGAFLVIGLFF
                     GLAELWAGRMRFVGLSHKERREYADRYKRDNNRTAPFSVLASLDSPGRMVRARRHVWNY
                     AYWLTGAAGLALLGGLVVAVFIESWGETVLRPDLYANADYYLLQPANLFGIVWCAGGLV
                     AGILLARLLAFLLLDGHFAAADEAVNAKLNQESRRSSQRTGEMTDVRHLHFGEPVPVNA
                     LADFSTEQARKQQAVFLGKDEQGQPVLVPRDTWRKTNIQILGLPGSGKSVMGTNALIRC
                     VRDFGDAVVYFDPKGDAWAPHVFRAHCPDFTLLDLRPGKPAQLNLFRDLDQYALKNLLV
                     AGFNLSETGDVADHYRISEQKAAKLIAEQFPQGANIQQVLAAAYALPEALKKDVKGLIT
                     KLENVADLSVLQTDSGIDVAGIINGGGCLYVIGSMDDEAVIRVQKMLFARCAQIIIARD
                     EFRRWPHASIMLDEIKYLLSKYVLNALGTLRSRDCNLLLAHQSLGDFGQCGQDLPAEFV
                     KTTVLDNTPIRWFYRAASQESAQWAAGQTGEIRVDVERRRASREAGNVEHISGDSFIQK
                     EARPLFDVNTLQHLPDGFAVMTGLGVARMAFSSPLRVERREIPLQRFPVLAKTDPLAEY
                     QPEAGRQRPGDDDFAGLY"
                     /name="TraM recognition domain-containing protein"
     gene            6170..6901
                     /gene="mobC"
                     /locus_tag="LCH45_RS28045"
                     /old_locus_tag="LCH45_28080"
     CDS             6170..6901
                     /gene="mobC"
                     /locus_tag="LCH45_RS28045"
                     /old_locus_tag="LCH45_28080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family replication-relaxation protein"
                     /protein_id="WP_071653262.1"
                     /translation="MALERYNVSHAKRQARNAEKTRLTLRWLREELCSTAELVARRLGI
                     AAVQPVYRFLDSLVAKGLLVRAKYPVDGRQVSVWGLTPHGVAFSFDEDEPLTDVIPFQP
                     SRVSAAQLPHRLAVQSLRLAMEARGASGWRYLHRMALKGMKVPDALAELDGRTVAFEVE
                     RTVKSRRRYQEVVSGYLFNRKANGIDEIWYICPDRATQVRVQRAILSVDEIVNPQTGEA
                     RKTAELDRERLFACFKFMTTE"
                     /name="mobC"
     gene            6908..7438
                     /locus_tag="LCH45_RS28050"
                     /old_locus_tag="LCH45_28085"
     CDS             6908..7438
                     /locus_tag="LCH45_RS28050"
                     /old_locus_tag="LCH45_28085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008321413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938511.1"
                     /translation="MALYMVFIEDAEDRDGERCIMPTSEHLEDMVVLAWQTGAYVFCFV
                     DRAQHLAMMKSIGMYDERLNSDEKATYFFTGKNPQWERDKPAGMKLCDYLYRKVERFAI
                     ENGVQVVDAGKCSPEQISAMIEVVSERHGESAVNEKLKAELMREYNSRYFNVPVPELPE
                     DKPARRWWPFGKK"
                     /name="hypothetical protein"
     gene            complement(7466..7645)
                     /locus_tag="LCH45_RS28055"
                     /old_locus_tag="LCH45_28090"
     CDS             complement(7466..7645)
                     /locus_tag="LCH45_RS28055"
                     /old_locus_tag="LCH45_28090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="WP_021515244.1"
                     /translation="MTKQEKTALNMAKFIKDQSLLLLEKLNELDMDTPADMCERLHEDA
                     ETLWQVMRDKLGKE"
                     /name="Rop family plasmid primer RNA-binding protein"
     gene            7714..8091
                     /locus_tag="LCH45_RS28060"
                     /old_locus_tag="LCH45_28095"
     CDS             7714..8091
                     /locus_tag="LCH45_RS28060"
                     /old_locus_tag="LCH45_28095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938513.1"
                     /translation="MAWVTVRQATELTGKARSSLYRDMAKGRVSYRTEADGGRVVDTSE
                     LIRVYGELRQIETHERDGLRLSDETEKMASDALIAEIKALREEVAGLRQEMQAMRLLEH
                     KPETAETPAGTSRRWWQWGKR"
                     /name="hypothetical protein"
     gene            complement(8153..8452)
                     /locus_tag="LCH45_RS28065"
                     /old_locus_tag="LCH45_28100"
     CDS             complement(8153..8452)
                     /locus_tag="LCH45_RS28065"
                     /old_locus_tag="LCH45_28100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009365129.1"
                     /GO_function="GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA]; GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /GO_process="GO:0030541 - plasmid partitioning [Evidence
                     IEA]; GO:0051290 - protein heterotetramerization [Evidence
                     IEA]"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="WP_063938515.1"
                     /translation="MPYEVEWKQGAIEDVTALFDYIAENSSLWDAQNVTDRVLAAADKL
                     ADFPRLYEVDSRYGEDVRRISLMGQHVLYDVDDQTRKVRVLAVVGQRQNPHTVR"
                     /name="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(8442..8705)
                     /locus_tag="LCH45_RS28070"
                     /old_locus_tag="LCH45_28105"
     CDS             complement(8442..8705)
                     /locus_tag="LCH45_RS28070"
                     /old_locus_tag="LCH45_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="WP_063938517.1"
                     /translation="MSNSTHYENANFLRELAERLPRILPEGGPDKAALLQRLANEELAQ
                     AEYEDQVRAKVTAARADTRPGMTTEQLRQRLHGRYQELRDAV"
                     /name="hypothetical protein"
     Typing          complement(2829..3409)
                     /name="ColRNAI_rep_cluster_1857"
                     /typing="replicon"
                     /tool="MOB-suite"
                     /identity="98.625"
                     /coverage="100.0"
                     /evalue="0.0"
     Typing          6171..6898
                     /name="MOBC"
                     /typing="relaxase"
                     /tool="MOB-suite"
                     /identity="95.885"
                     /coverage="100.0"
                     /evalue="3.84e-144"
ORIGIN
        1 gctatgaaat aaaaaaacgg tttgttgtag agcctgagaa aaaacacttt ttccttaatg
       61 taagtctgga tgaaaaaaac ggacgtgcat ttattaccga cccggatatt cccggggtgc
      121 tggttgttga tgtaaactcg ggaaaaatac tacacagaat tgatgttata aattcactgg
      181 cgattttatt taatccttta cggaacgaaa tctatatcac gcaccgtaat gcaaaacagg
      241 tcagcattgt ggacagtaat tcttattacg taaaaacaac gataaaaacc cgcctgatgc
      301 caaacagtct gtctttatca catgacggtg aaaatctgta cgttagtgta aaacaagaaa
      361 aaaaagagat gagtactatg aaggattaca tactcaaaat cgaattatct aaaattgcta
      421 tggagccaga agaatgaata aatatttagg gctgagcata gtatatatga tgtatacatc
      481 atctgttatg gctgatgatt ctggcgcagt tagtaaagat aaaaatattt caccagaaga
      541 aacattaatt gttcatggga ccgctgtttc tgaaagcaca acagaaggaa cagattcata
      601 cacaacccag tcagccaata cagcatcaaa gatgaatctg tcattccggg atacacctca
      661 gtcaactaca gtcattacgc gacgaagaat ggatgatgta gcagcaaaaa atattattga
      721 tgtggttaaa accgctcctg gattatatgt cagtacagca gaaggttccg gaagaccgaa
      781 ttttatgtcc agaggattta atgctaatgt catgtatgaa ggatttagcc tgccgtggaa
      841 ttcatacatt ccaagttcac aaactagttc agctattttt gaccgggtgg agatagtccg
      901 cggggcaaca ggtcttgttc agggtgccgg agacccttca gccgcgataa atatgataca
      961 caagcgtccg acccggcaat atcaggccag cactaacatc ggtgccggac ggtggaatga
     1021 ctaccggggt ggggttgatg tatcaggacc actgaatgac agcgcgacct tacgcggaag
     1081 ggcagttgtg gatggcgaaa acagtgagac cttcagggat agtgagaaac gcaggaataa
     1141 cgtcatttac ggaattatgg aggcagatct gactgataat accctactta cgattggggg
     1201 atatcagcag tatgaatata acaatcattg gtggtatgac ttacctattt ctggtaccgg
     1261 acaccacctt aatcttcctc gctcgacgtt tacaggcaat gactgggagt ttgaaagaag
     1321 taaaagtaaa actgcatttg caactcttga gcagaagttc agtaatagct ggaagctgaa
     1381 attttcaacg ctgcaaaact ggagaaatgc tgacctgtta gggacggcaa cataccggca
     1441 taaagatacc cgacaaagta tgtatcagca agtatgggga tcactgaggg aatataattc
     1501 caaaagctat gacctgtcgc ttaatgggga ctatgcttta tttggtcgtg ttcatcaatt
     1561 cgtggctgga acaactcagc aaaaatacac cgataaagat acaggttata catattcccc
     1621 aatatatatt gagaatgtta atatatttga tcatggttca cattcttcga aaaaacccat
     1681 gaagatgtca ggtgatgcat cctacgacgt cactgaacaa aaaagcgcct acgcagcaac
     1741 tcattttcta cttagtgata actggaaaat attgctgggt ggccgtcttg actggtatga
     1801 atatacaagt aaaggtacat ccattaatac aggttataaa gttaccagaa atatcaccaa
     1861 atatgcaggt tcaacataca atcttgacga tcatcatacc atttatggga gctataccga
     1921 tatattcaat ccacagtcaa gcaccgacct caatggtaaa gtgatcaagc ctattgttgg
     1981 taagaattat gagacaggta ttaagggtga gtattttaac ggcttgctta atgcctctct
     2041 ggcggtattt caggtagacc aggaaaatcg agccaggctg cttcccgatc agacagggtg
     2101 tgttactaat ggcaatcctc aatgctatga ggctagcggt aaagtacgca caaaagggat
     2161 agatttcgaa cttcagggca tggttacgcc actctgggaa attggggtgg gatatacata
     2221 tgcagatacg cgatatataa aagatggaga tcgtagtaat gaaggcaagc gattcaatac
     2281 tgccgtgtcg ccacaaaatt tatttaagat ttcgagcatg tacacctttg acggaaaatt
     2341 aacaaactgg cgagcaggag taaacacgta ctggcaaagt aaaatatatg ctaacataac
     2401 agacacaagg gatggcaatc cggttattaa taaacaaaag gcttattcaa caactgacat
     2461 tattattggc tatactccaa tgccacaact ggatttacag ctcaatataa ctaatgtctt
     2521 tgataaggtt tattatacat ccataggtga atcgattgga tatggcacgg aaacatatgg
     2581 agaaccaaga aatgttaaaa ttagtgcaac ctatagattc taaccatgcg gattgggtct
     2641 tctcctcgga tttgccccta taaatccgtc ttttgtggtt ccggcgtaca accccctgtt
     2701 tgccacttca gttgatggtt aatcaaatca tgtagaggat cttcttgaga tcctccatgg
     2761 ttcgtcgtaa tcttttgccc tgtaaacgaa aaaaccacct ggggaggtgg tttgatcgaa
     2821 ggttaagtca gttggggaac tgcttaaccg tggtaactgg ctttcgcaga gcacagcaac
     2881 caaatctgtc cttccagtgt agccggactt tggcgcacac ttcaagagca accgcgtgtt
     2941 tagctaaaca aatcctctgc gaactcccag ttaccaatgg ctgctgccag tggcgttttt
     3001 gcgtgtcttt ccgggttgga ctcaagtgaa cagttaccgg ataaggcgca gcagtcgggc
     3061 tgaacgggga gttcttgctt acagcccagc ttggagcgaa cgacctacac cgagccgaga
     3121 taccagtgtg tgagctatga gaaagcgcca cacttcccgt aagggagaaa ggcggaacag
     3181 gtatccggta aacggcaggg tcggaacagg agagcgcaag agggagcgac ccgccggaaa
     3241 cggtggggat ctttaagtcc tgtcgggttt cgcccgtact gtcagattca tggttgagcc
     3301 tcacggctcc cacagatgca ccgaaaaagc gtctgtttat gtgaattctg gcaggagggc
     3361 gaagcctatg gaaaaacgcc accggcgcgg ccctgccgtt ttgcctcaca tgttagtccc
     3421 ctgcttatcc acggaatctg tgggtaactt tgcgagagtt agcagcgccc gccgcagtct
     3481 cacggccgga gcgtagcgac tgagtgaggg aggaggcgca atatcccccc tcccgcagac
     3541 ggccaccgga cgcgcggatg tgacatattg caccgtgtta ccgcctttcc cttccctgcc
     3601 gataaatcat tcagataggt gaagtctgtc ggttttctgc tgaatatttt acgttaacct
     3661 atctctgttg cttttttctc cttgcggaga aatgcaggtc atccccgctg gggcgtggct
     3721 gacttcagga ggaaatcccc tgcttacgct ggctggacgc caccgacgca cggtctttcc
     3781 ccctctttct ggctcagctc ggctggacgc cggccacgcc agaaattcac ccggcccccg
     3841 tcaggctcac gcctgaccac ccacaaaaac ccacggtgct gaacgcacct gacgaagcag
     3901 caaagctgct cctgattttg cgggtggtca gccgctcgct atggcgaact ctgacggggg
     3961 ccggaacacc ggaaaagacc gcactggggt gccgcctttt cctctcacac gctggggcgt
     4021 gctcgttgtt cctccggggc tgcgcctttt ccgcttgggg cggaaagagg ctggcctccg
     4081 tggtgaaagc tgaatgcaat caccacggac gtgaccgccg ccgccatgcc tcggggcatg
     4141 tccgcggctt gtgcagcagg ggctgcacac cacgccggta aacccgtcgt ctgaaggaga
     4201 gagattgcat gtttaatacg gattcgcttg cctggcagtg gcatatcccg gagctggccc
     4261 ggcagattta cccgtgggtc agctggctgt acagggagta ttacggtctg gccggggctt
     4321 tcctggttat cggcctgttt ttcggtctgg ctgaactctg ggccggacgg atgcgttttg
     4381 tggggctgag tcataaggag cgacgggagt atgccgatcg ctataagcgc gataataacc
     4441 ggactgcgcc attttcggtg ctggcttcgc tggattcgcc cgggcggatg gtcagggcac
     4501 ggcggcatgt ctggaactac gcgtactggc tgaccggcgc tgccggtctg gcccttctgg
     4561 gggggctggt tgtcgccgtg tttatcgaga gctggggaga aaccgtcctg cgtccggacc
     4621 tctacgcgaa cgcagattat tacctgctgc agccggctaa cctgttcggg attgtctggt
     4681 gtgccggagg gcttgtcgcc ggcatccttc tggcgcggct gctggcattt ctgctgcttg
     4741 acggtcattt tgcggccgct gacgaggcgg tgaatgcgaa actgaaccag gagagccggc
     4801 gcagcagcca gcggaccgga gaaatgaccg acgtccgcca cctgcatttc ggggagccgg
     4861 taccggttaa cgcgctggcc gatttttcca cagagcaggc ccggaaacag caggcggtgt
     4921 ttctgggtaa ggatgagcag ggccagccgg tgctggtccc gcgtgatacc tggcgcaaga
     4981 ccaatattca gatcctcggc ctgcccggga gcggcaagag cgtgatgggc accaacgcgc
     5041 tcattcgctg cgtacgcgat ttcggggacg cggtggtgta cttcgacccc aaaggggacg
     5101 cgtgggcgcc gcacgtcttc cgggcgcact gcccggactt caccctgctg gatctgcggc
     5161 cggggaaacc ggcgcagctg aatctgtttc gcgatctcga ccagtacgcc ctgaagaatc
     5221 tgctggttgc cgggtttaac ctgagtgaga ccggcgacgt ggcggaccac taccgcatca
     5281 gcgagcagaa agcggcgaag ctgattgcgg agcagttccc gcagggggca aacattcagc
     5341 aggtgctggc ggcggcatac gcgctgccgg aggccctgaa aaaggacgtg aaggggctga
     5401 tcaccaagct ggagaacgtg gccgacctga gcgtcctgca gacggacagc ggcatcgacg
     5461 tggcggggat aatcaacggc ggcggctgcc tgtacgtcat cggctcgatg gacgatgagg
     5521 cggtgatccg ggtgcagaag atgctgtttg cccgctgcgc gcaaatcatc attgcgcggg
     5581 atgagttccg gaggtggccg cacgcgagca tcatgctcga tgagattaaa tacctgctgt
     5641 cgaagtacgt gctgaacgcg ctggggacgc tgcgcagtcg ggactgcaac ctgctgctcg
     5701 cgcaccagtc gctgggcgac ttcgggcagt gcggccagga tctgcccgct gagtttgtga
     5761 aaaccacggt gctggataac acgccgatcc gctggttcta ccgggcggcc agccaggagt
     5821 ccgcgcagtg ggcggcgggc cagaccgggg aaatccgggt ggacgtggag cgccgccggg
     5881 ccagccggga ggcggggaac gtggagcata tcagcgggga cagcttcatt cagaaggagg
     5941 cgcggccgct gtttgacgtg aacaccctgc agcacctgcc tgacgggttt gcggtgatga
     6001 ccgggctggg cgtggcccgg atggcgttca gcagcccgct gcgggttgaa cgccgggaaa
     6061 tcccgctgca gcgcttcccg gtgctggcga aaacggaccc gctggcggag taccagccgg
     6121 aggccgggcg gcagcggccg ggtgatgatg attttgcggg gctgtactga tggcactgga
     6181 gcgatacaac gtcagccacg cgaagcggca ggcgcggaac gcggagaaaa cccggctgac
     6241 cctgcgctgg ctgcgggaag agctgtgttc cacggcggag ctggttgcgc gacggctggg
     6301 gattgcggcg gtgcagccgg tttaccgctt cctggacagc ctggtggcga aagggctgct
     6361 ggtccgggcg aaatacccgg ttgacggccg tcaggtcagc gtgtggggac tgacgccgca
     6421 cggggtggcg ttctcgttcg acgaggacga accgctgacg gacgttattc cgtttcagcc
     6481 gtcaagggtg tcggcggcgc agctgccgca ccggctggcc gtgcagtccc tgcgtctcgc
     6541 catggaggcc cggggggcct ccggctggcg gtacctgcac cgtatggccc tgaaggggat
     6601 gaaggtgccg gacgcgctgg cggagctgga cggcagaacg gtggcgtttg aggtggagcg
     6661 caccgtgaag agccgcaggc gctatcagga ggtggtgtcc gggtatctgt tcaatcgcaa
     6721 agcgaacggc atcgatgaaa tctggtacat ctgcccggac cgggcgacgc aggtcagggt
     6781 gcagcgggcg atcctgtcgg tggatgaaat cgtgaatcca cagaccggcg aggcccgcaa
     6841 aacggcggag ctggaccggg agcggctgtt tgcgtgcttt aaatttatga caacggagtg
     6901 agggattatg gcgctgtaca tggtatttat tgaagacgcg gaggaccggg acggcgagcg
     6961 ctgcatcatg ccgaccagcg agcatcttga agatatggtg gtgctcgcct ggcagaccgg
     7021 ggcgtatgtt ttctgttttg tcgaccgggc ccagcatctg gcgatgatga aatccatcgg
     7081 gatgtatgac gagcggctca acagcgacga aaaagccacg tactttttca ccggtaaaaa
     7141 tccgcaatgg gaacgggaca aacccgcagg aatgaagctg tgtgattacc tctatcgcaa
     7201 ggtggagcgg ttcgccatcg agaacggtgt gcaggtggtt gatgccggga agtgctcacc
     7261 ggagcagatt agcgcgatga ttgaggtggt cagcgagcgg cacggggaaa gcgcggtgaa
     7321 cgagaagctg aaagcggagc tgatgcggga atataacagc cgctatttta acgtgccggt
     7381 gcctgaactg ccggaggaca aacccgcccg ccggtggtgg ccgttcggca aaaaataaac
     7441 ggcagcaccg gccatgccgg tgctgttatt ccttcccgag cttatcccgc atcacctgcc
     7501 agagtgtttc cgcatcctcg tgcagccgct cgcacatgtc ggccggggta tccatatcca
     7561 gctcgttcag tttctcgagt aacagcagcg actggtcctt aataaattta gccatattga
     7621 gggcagtttt ttcctgctta gtcatgtttt ctgtccttcc gggtgtggtg tgcttcctgt
     7681 cgggtatact gtctcaaaac cggaggtgag acaatggcgt gggtgacggt gagacaggcg
     7741 acggaactga cggggaaagc ccggagttcc ctgtataggg atatggcgaa aggccgcgta
     7801 tcataccgta ctgaagctga tggtggtcgg gtggtggata ccagcgaact tatccgggtg
     7861 tacggtgaat taagacagat tgagactcac gagcgggacg ggttgagact gtcggatgag
     7921 actgaaaaaa tggcctccga cgcgttgatc gcggagataa aagcactcag ggaggaggtg
     7981 gccggactgc ggcaggaaat gcaggccatg agactgctgg aacacaaacc agagacagca
     8041 gagacaccgg ccggaacatc gcgccgatgg tggcagtggg gaaaacgctg acgcgctgtg
     8101 cttgtccaac cggcgacggc cacgcaatga attgcgcccc catcaccggt tatcagcgaa
     8161 cggtatgggg attctggcgc tggccaacca cggccagaac ccggactttc ctggtctggt
     8221 catcaacatc atagaggaca tgctgtccca tcagactgat ccggcgaacg tcctcgccat
     8281 agcgtgagtc cacttcatat aggcgcggga agtcggccag tttgtccgct gcagcgagaa
     8341 ctcgatcggt aacgttctgc gcatcccaca gggatgaatt ttccgcaatg tagtcaaaca
     8401 atgctgtcac atcctcgatg gctccctgct tccattccac ctcatacggc atcgcgtagt
     8461 tcctgatagc ggccatgcag tctctggcgg agttgttccg tggtcatgcc cgggcgcgtg
     8521 tcagcgcggg cggcggtcac tttagcgcga acctgatctt catattctgc ctgggccagt
     8581 tcttcgttag ccaggcgctg caacagtgca gctttgtccg ggccaccttc aggcagtatg
     8641 cggggcagtc tctcggccag ttcccgcagg aaattggcgt tttcatagtg tgtactgttg
     8701 ctcattgtgg agcctcccgg gctgatacgt cgatatgaag gagtttagca gggggcggtc
     8761 tgaaagaata tgctcgtgtc agtcaggctg atgaagacga aagctggtaa aaaagggctt
     8821 acgacaccca ttttacgtca aactgcacga catgcagttg cgtgcatagc catgcagcgg
     8881 gttaaaattt atcaggcgcg tttccggcgg ttttccgggt ggtttgttgc cggttttatc
     8941 ccgtagccgc cggaaatgcc ctcagaccgt ctgagcggtg cgcgtaatgc gacgttatgg
     9001 taaatcatgt gattaatggt agtgataatc attatcactt ttgctatatt cttgtttctt
     9061 ttattgtaag gaaatgtaag tatggcaatc acgattaaat ccgccgttaa tgtccgtagc
     9121 agagtattaa tatactcttt actactgttt actggttcag cgtatgcggc tgatatagct
     9181 gaatcaacat tcctgactcg tgatatcggc aatggtctgt atgaaatgtc ttttgatggc
     9241 aaaaataata gtctctgggc agcatcatcg tcttcattcg acagagataa gggccacgga
     9301 attgtttatc agttagaacc atccactctg aaaacaaaat ctgccgttaa aatcgcacgt
     9361 cttgggtttg ccacagcact ggatgaggaa catgagcgac tttatatcgg caatactctg
     9421 gacggctcag tgacggtcat tgatactcta accggaaaag aactttcagt aattccgtta
     9481 actgagggac aggatgaggc tcatttcaga ccgaccagag aaatggtcat tgacaaaaaa
     9541 aatcagcgtc tttatgtctc cagcgccgca gataaagggg ctctgtgggt tattgacatg
     9601 gctgtcggga agaaaattaa aaccattgat ggactggggc acgctgttac tggaattgcg
     9661 ttaaataaaa atggggataa tatttatctg gttaacgggg atggtgagat tattaacctg
     9721 gatgccagta
//
